Name: Escherichia coli 824-DH
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 4916

Type strain:

Species: Escherichia coli

Strain Designation: 824-DH

Culture col. no.: DSM 22311, CIP 105836

Strain history: <- C. Bizet, CIP <- G. Prats, Hospital de Sant Pau, Barcelona, Spain; 824-DH

NCBI tax ID(s): 562 (species)

SI-ID 68434

CIP 105836, DSM 22311

Special Collections

DSMZ
CIP

Other strains from Escherichia coli

Section

Escherichia coli 824-DH is a human pathogen that has multiple antibiotic resistances and was isolated from human blood.

antibiotic resistance
human pathogen
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Escherichia*
	Species	**Escherichia coli**
	Full Scientific Name (LPSN)	Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

Synonym

"Bacillus coli"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16272]

Culture medium

LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)

[Ref.: #16272]

Culture medium growth

[Ref.: #16272]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16272]

Culture medium composition

expand / minimize

[Ref.: #41756]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #41756]

Culture medium growth

[Ref.: #41756]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16272]			growth	30 °C
[Ref.: #41756]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Reference

[Ref.: #16272]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
resistant0
resistant0
resistant0
resistant0
resistant0
10
14-16
36-38
34
34-36
28
24
20
22
resistant0
12
resistant0
34
34-36
14-16
28
24-26
resistant0
14-16
34
18
26-28
34
resistant0
34-36
34
resistant0
resistant0
resistant0
resistant0
8

Only first 15 entries are displayed. Click here to see all.

[Ref.: #16272]

Name of produced compound

SHV-1 beta lactamase

[Ref.: #16272]

Observation

ESBL

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #16272]	17583	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16272]	Sample type/isolated from	human blood
[Ref.: #16272]	Geographic location (country and/or sea, region)	Barcelona
[Ref.: #16272]	Country	Spain
[Ref.: #16272]	Country ISO 3 Code	ESP
[Ref.: #16272]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Fluids	#Blood

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16272]	Pathogenicity (human)	yes
[Ref.: #16272]	Pathogenicity (animal)	yes
[Ref.: #16272]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Availability in culture collections External links

[Ref.: #16272]

Culture collection no.

DSM 22311, CIP 105836

[Ref.: #74414]

SI-ID 68434

References

#16272

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22311

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41756 ; Curators of the CIP;

#68368

Automatically annotated from API 20E .

#74414

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID68434.1 )

* These data were automatically processed and therefore are not curated

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