Name: Escherichia coli B
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 4419

Type strain:

Species: Escherichia coli

Strain Designation: B, B (Luria)

Culture col. no.: DSM 613, ATCC 11303, CCUG 2468, JCM 20137, CGMCC 1.8486, IAM 1268, NCIMB 11595, CIP 103914, CCM 7372, NRC 745

Strain history: CIP <- 1993, ATCC <- S.E. Luria: strain B

NCBI tax ID(s): 562 (species)

SI-ID 9967

LMG 17555, ATCC 11303, CIP 103914, LMG 17664, NCIMB 11595, NCIB 11595, CCUG 2468, IAM1268, CECT 4201, CCRC 10674, CCRC 13055, BCRC 10674, BCRC 13055, BCRC 14893, CCRC 14893, CCT 7443, NRRL B-14943, CNCTC 6241, JCM 20137, CCM 7372, DSM 613

Special Collections

Escherichia phage T4 (Escherichia virus T4) Escherichia phage T3 (Escherichia virus T7) Escherichia phage T6 (Escherichia virus T4) Escherichia phage T7 (Escherichia virus T7) Escherichia phage T1 (Escherichia virus T1) Escherichia phage T2 (Escherichia virus T4) Escherichia phage T5 (Escherichia virus T5) Escherichia phage vB_EcoP-PTXU04 (Escherichia virus PTXU04) Escherichia phage vB_EcoS-PT-GWK02

Other strains from Escherichia coli

Section

Escherichia coli B is a facultative anaerobe, Gram-negative, rod-shaped bacterium that has multiple antibiotic resistances.

antibiotic resistance
Gram-negative
rod-shaped
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Escherichia*
	Species	**Escherichia coli**
	Full Scientific Name (LPSN)	Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

Synonym

"Bacillus coli"

Information on morphological and physiological properties Morphology

[Ref.: #118681]	Gram stain	negative

[Ref.: #118681]	Cell shape	rod-shaped

[Ref.: #118681]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #321]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #321]

Culture medium growth

[Ref.: #321]

Culture medium link

Medium recipe at MediaDive

[Ref.: #321]

Culture medium composition

expand / minimize

[Ref.: #41709]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #41709]

Culture medium growth

[Ref.: #41709]

Culture medium composition

expand / minimize

[Ref.: #118681]

Culture medium

CIP Medium 72

[Ref.: #118681]

Culture medium growth

[Ref.: #118681]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #118681]

Culture medium

CIP Medium 3

[Ref.: #118681]

Culture medium growth

[Ref.: #118681]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #321]	growth	37 °C
[Ref.: #41709]	growth	30 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #118681]	growth	5-41 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118681]

Oxygen tolerance

facultative anaerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Reference

[Ref.: #321]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
12
resistant0
28-30
36
28
26-28
30-32
44
48
36
30
36
26-28
28-30
8
10
resistant0
42-44
42
16
36
40
resistant0
16
38
24
26
44
resistant0
42
40
resistant0
8
resistant0
10
30-32

Only first 15 entries are displayed. Click here to see all.

[Ref.: #321]	Name of produced compound	L asparaginase
[Ref.: #321]	Name of produced compound	aspartate aminotransferase
[Ref.: #321]	Name of produced compound	aspartate transaminase
[Ref.: #20216]	Name of produced compound	L-asparaginase

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #118681]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #118681]	esculin ChEBI	+	hydrolysis
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #118681]	glucose ChEBI	+	fermentation
[Ref.: #118681]	glucose ChEBI	+	degradation
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #118681]	lactose ChEBI	+	fermentation
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #118681]	malonate ChEBI	-	assimilation
[Ref.: #118681]	mannitol ChEBI	+	fermentation
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #118681]	nitrate ChEBI	+	reduction
[Ref.: #118681]	nitrite ChEBI	+	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #118681]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #118681]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite	Production
[Ref.: #118681]	indole ChEBI	yes
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #118681]	acetoin ChEBI	-
[Ref.: #118681]	glucose ChEBI		+
[Ref.: #68368]	acetoin ChEBI	-			* from API 20E
[Ref.: #68368]	indole ChEBI			+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118681]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #118681]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #118681]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118681]	gelatinase	-
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118681]	lysine decarboxylase	+	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118681]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #118681]	oxidase	-
[Ref.: #118681]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #118681]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #118681]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #321]	17720	+	-	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #321]	17719	+	-	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #321]	16946	+	-	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #321]	16945	+	-	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #321]	16944	+	-	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #321]	16943	+	-	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #321]	16942	+	-	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118681]	508	not determinedn.d.	+	+	+	+	+	-	+	-	-	+	+	+	+	+	+	-	-	+	-	+	+	+	-	+	-	-	-	-	-	+	-	+	-	+	-	-	+	-	+	-	-	-	-	+	+	-	-	+	+	+	-	-	-	-	+	+	-	+	-	+	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	+	-	-	-	+	+	-	+	+	-	-	+	-	-	+	-	+	+	+	+	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118681]	7737	-	+	+	+	-	+	-	-	+	-	+	+	+	+	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence LC595312 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	5613
Soil Samples	2111
Animal Samples	88071
Plant Samples	1835
Total Samples	97630

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #321]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118681]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Escherichia coli JCM:20137 gene for 16S rRNA, partial sequence	LC595312	1464	GenBank ENA	562 tax ID

Availability in culture collections External links

[Ref.: #321]

Culture collection no.

DSM 613, ATCC 11303, CCUG 2468, JCM 20137, CGMCC 1.8486, IAM 1268, NCIMB 11595, CIP 103914, CCM 7372, NRC 745

[Ref.: #73909]

SI-ID 9967


[Ref.: #124042]	Escherichia phage T4 (Escherichia virus T4)
[Ref.: #124042]	Escherichia phage T3 (Escherichia virus T7)
[Ref.: #124042]	Escherichia phage T6 (Escherichia virus T4)
[Ref.: #124042]	Escherichia phage T7 (Escherichia virus T7)
[Ref.: #124042]	Escherichia phage T1 (Escherichia virus T1)
[Ref.: #124042]	Escherichia phage T2 (Escherichia virus T4)
[Ref.: #124042]	Escherichia phage T5 (Escherichia virus T5)
[Ref.: #124042]	Escherichia phage vB_EcoP-PTXU04 (Escherichia virus PTXU04)
[Ref.: #124042]	Escherichia phage vB_EcoS-PT-GWK02

References

#321	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 613

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#41709 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#73909

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID9967.1 )

#118681 Collection of Institut Pasteur ; Curators of the CIP; CIP 103914

#124042

Dr. Johannes Wittmann, Dr. Clara Rolland, Dr. Lorenz Reimer, Joaquim Sardà: PhageDive .

* These data were automatically processed and therefore are not curated

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