Strain identifier

BacDive ID: 4419

Type strain: No

Species: Escherichia coli

Strain Designation: B, B (Luria)

Strain history: CIP <- 1993, ATCC <- S.E. Luria: strain B

NCBI tax ID(s): 562 (species)

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General

@ref: 321

BacDive-ID: 4419

DSM-Number: 613

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped, antibiotic resistance

description: Escherichia coli B is a facultative anaerobe, mesophilic, Gram-negative bacterium that has multiple antibiotic resistances.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

@refhistory
321<- ATCC <- S.E. Luria, B
67770IAM 1268 <-- ATCC 11303 <-- S. E. Luria strain B.
118681CIP <- 1993, ATCC <- S.E. Luria: strain B

doi: 10.13145/bacdive4419.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 321

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: B, B (Luria)

type strain: no

Morphology

cell morphology

  • @ref: 118681
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 118681

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
321NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
41709MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118681CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
118681CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
321positivegrowth37mesophilic
41709positivegrowth30mesophilic
67770positivegrowth37mesophilic
118681positivegrowth5-41

Physiology and metabolism

oxygen tolerance

  • @ref: 118681
  • oxygen tolerance: facultative anaerobe

antibiogram

  • @ref: 321
  • medium: Mueller-Hinton Agar
  • Penicillin G: 12
  • oxacillin: 0
  • ampicillin: 28-30
  • ticarcillin: 36
  • mezlocillin: 28
  • cefalotin: 26-28
  • cefazolin: 30-32
  • cefotaxime: 44
  • aztreonam: 48
  • imipenem: 36
  • tetracycline: 30
  • chloramphenicol: 36
  • gentamycin: 26-28
  • amikacin: 28-30
  • vancomycin: 8
  • erythromycin: 10
  • lincomycin: 0
  • ofloxacin: 42-44
  • norfloxacin: 42
  • colistin: 16
  • pipemidic acid: 36
  • nitrofurantoin: 40
  • bacitracin: 0
  • polymyxin b: 16
  • kanamycin: 38
  • neomycin: 24
  • doxycycline: 26
  • ceftriaxone: 44
  • clindamycin: 0
  • fosfomycin: 42
  • moxifloxacin: 40
  • linezolid: 0
  • nystatin: 8
  • quinupristin/dalfopristin: 0
  • teicoplanin: 10
  • piperacillin/tazobactam: 30-32

compound production

@refcompound
321L asparaginase
321aspartate aminotransferase
321aspartate transaminase
20216L-asparaginase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis
11868129864mannitol+fermentation
11868116947citrate-carbon source
1186814853esculin+hydrolysis
11868117234glucose+fermentation
11868117716lactose+fermentation
11868117632nitrate+reduction
11868116301nitrite+reduction
11868115792malonate-assimilation
118681132112sodium thiosulfate-builds gas from
11868117234glucose+degradation

antibiotic resistance

  • @ref: 118681
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
11868135581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole+
11868115688acetoin-
11868117234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
118681oxidase-
118681beta-galactosidase+3.2.1.23
118681alcohol dehydrogenase+1.1.1.1
118681gelatinase-
118681catalase+1.11.1.6
118681lysine decarboxylase+4.1.1.18
118681ornithine decarboxylase-4.1.1.17
118681phenylalanine ammonia-lyase-4.3.1.24
118681tryptophan deaminase-
118681urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118681-+++-+--+-+++++++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
321+-+-----+--++-++-+-+-
321+-+-----+--++-++-+-+-
321+-+-----+--++-++-+-+
321+-+-----+--++-++-+-+
321+-+-----+--++-++-+-+-
321+-+-----+--++-++-+-+-
321+-+-----+--++-++-+-+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118681+++++-+--++++++--+-+++-+-----+-+-+--+-+----++--+++----++-+-++-------++-----+---++-++--+--+-++++-+--

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_8.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_7;99_8&stattab=map
  • Last taxonomy: Enterobacteriaceae
  • 16S sequence: LC595312
  • Sequence Identity:
  • Total samples: 97630
  • soil counts: 2111
  • aquatic counts: 5613
  • animal counts: 88071
  • plant counts: 1835

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
3211Risk group (German classification)
1186811Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Escherichia coli JCM:20137 gene for 16S rRNA, partial sequence
  • accession: LC595312
  • length: 1464
  • database: ena
  • NCBI tax ID: 562

External links

@ref: 321

culture collection no.: DSM 613, ATCC 11303, CCUG 2468, JCM 20137, CGMCC 1.8486, IAM 1268, NCIMB 11595, CIP 103914, CCM 7372, NRC 745

straininfo link

  • @ref: 73909
  • straininfo: 9967

Reference

@idauthorscataloguedoi/urltitle
321Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 613)https://www.dsmz.de/collection/catalogue/details/culture/DSM-613
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
41709Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15764
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73909Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9967.1StrainInfo: A central database for resolving microbial strain identifiers
118681Curators of the CIPCollection of Institut Pasteur (CIP 103914)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103914