Name: Escherichia coli Seattle 1946
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 4427

Type strain:

Species: Escherichia coli

Strain Designation: Seattle 1946

Culture col. no.: DSM 1103, ATCC 25922, NCIB 12210, WDCM 00013, CCUG 17620, CCUG 7736, CCUG 21456, JCM 5491, CCM 3954, CIP 76.24, IFO 15034, KCTC 1682, LMG 8223, NBRC 15034, NCIMB 12110

Strain history: Y. Kosako <-- R. Sakazaki <-- ATCC 25922 <-- FDA strain Seattle 1946 <-- F. Schoenknecht.

NCBI tax ID(s): 1322345 (strain), 1236527 (strain), 562 (species)

SI-ID 2805

LMG 8223, ATCC 25922, NCIMB 12210, NCIB 12210, CCUG 17620, CCUG 7736, CCUG 21456, JCM 5491, NCIMB 12110, CCM 3954, IFO 15034, CIP 76.24, CECT 434, CCRC 14902, KCTC 1682, NBRC 15034, NCTC 12241, CNCTC 5276, NCCB 89159, LMD 89.159, BCRC 11509, CCRC 11509, BCRC 14902, BCRC 17320, CCRC 17320, CCT 0548, CCT 1457, ICMP 10151, NBIMCC 3702, NCIM 2931, CDBB 1020, ACM 1901, ACM 4789, CGMCC 1.2340, CGMCC 1.2385, VTT E-97835, DSM 1103

Special Collections

Other strains from Escherichia coli

Section

When using BacDive for research please cite our paper

Escherichia coli Seattle 1946 is a mesophilic human pathogen that has multiple antibiotic resistances and was isolated from clinical isolate.

antibiotic resistance
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Escherichia*
	Species	**Escherichia coli**
	Full Scientific Name (LPSN)	Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

Synonym

"Bacillus coli"

Information on morphological and physiological properties Morphology

[Ref.: #125438]	Gram stain	negative Confidence in %99.75

Information on culture and growth conditions Culture and growth conditions

[Ref.: #552]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #552]

Culture medium growth

[Ref.: #552]

Culture medium link

Medium recipe at MediaDive

[Ref.: #552]

Culture medium composition

expand / minimize

[Ref.: #41789]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #41789]

Culture medium growth

[Ref.: #41789]

Culture medium composition

expand / minimize

[Ref.: #552]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #552]

Culture medium growth

[Ref.: #552]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #552]	growth	37 °C
[Ref.: #41789]	growth	30 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Test 5

Test 6

Test 7

Reference

[Ref.: #552]
Mueller-Hinton Agar
37
1
aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
20
28
24
18
26
34
34
30
28
28-30
22-24
24-26
resistant0
10
resistant0
32-34
34
14
28
24
resistant0
14-16
22
20-22
22
34
resistant0
32
32
6
resistant0
resistant0
resistant0
24

[Ref.: #552]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
18-20
30
24-26
18
26-28
36
34-36
34
28-30
30
24
24
8
10
resistant0
36
38
14
28-30
24
resistant0
16
22
20-22
22
34
resistant0
34
34-36
6-8
resistant0
resistant0
resistant0
26

[Ref.: #552]
Mueller-Hinton Agar
33
1
aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
18
30
24
20
28
38
38
34
30
24-26
22
22
resistant0
10
8
40
40
14-16
32
26
resistant0
16
22
18
24-26
36
resistant0
32-34
38-40
resistant0
resistant0
resistant0
resistant0
26

[Ref.: #552]
Mueller-Hinton Agar
30
1
aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
18-20
30
26
20
30
38
38
36
30
30
24
24
resistant0
14
resistant0
40-42
42
14
30-32
24-26
resistant0
16
24
20
24
38
resistant0
32
40
6
resistant0
resistant0
resistant0
26-28

[Ref.: #552]
Mueller-Hinton Agar
37


yes
Inhibition zone diameter in mm
resistant0
resistant0
18
30
28
22
32-34
40
40
38
30-32
28
24-26
24-26
10
12-14
resistant0
38
38
16-18
30
26
resistant0
16
24-26
20
26
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #552]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
18
28
24
18
26
34
34
34
26
24
20
20
resistant0
6
resistant0
32
32
14
28
24
resistant0
16
20
18
20
36
resistant0
32-34
30
6
resistant0
6
resistant0
26

[Ref.: #552]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
18
28
24
18
26
34
34
34
26
24
20
20
resistant0
6
resistant0
32
32
14
28
24
resistant0
16
20
18
20
36
resistant0
32-34
30
6
resistant0
6
resistant0
26

Only first 15 entries are displayed. Click here to see all.

[Ref.: #552]

Name of produced compound

extended spectrum ß-lactamase ESBL

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #46634]

Fatty acid	Percentage	ECL
C16:1 ω7c	24.5	15.819
C16:0	18.9	16
C18:1 ω7c /12t/9t	12.9	17.824
C14:0 3OH/C16:1 ISO I	10.2	15.485
C14:0	8.3	14
C15:0	8.1	15
C17:0 CYCLO	5.3	16.888
C12:0	4.1	12
C13:0 3OH/C15:1 i I/H	2.3	14.469
C17:0	1.8	17
C13:0	1.7	13
Unidentified	1.1	16.301
C17:1 ω8c	0.9	16.792

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #552]	19614	+	+	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18639	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18640	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18641	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18642	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18645	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18646	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18647	-	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18649	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	18650	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	19404	+	-	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #552]	19965	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #552]	Sample type/isolated from	clinical isolate

[Ref.: #46634]	Sample type/isolated from	Human
* marker position based on {}

Isolation sources categories

#Infection	#Medical environment	#Clinic
#Infection	#Patient	-
#Infection	#Patient	#Specimen

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KC429776 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	5613
Soil Samples	2111
Animal Samples	88071
Plant Samples	1835
Total Samples	97630

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #552]	Pathogenicity (human)	yes
[Ref.: #552]	Pathogenicity (animal)	yes
[Ref.: #552]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Escherichia coli gene for 16S rRNA, partial sequence, strain: JCM 5491	AB594752	1464	GenBank ENA	562 tax ID
[Ref.: #552]	Escherichia coli partial 16S rRNA gene, strain DSM 1103	HE978270	1501	GenBank ENA	562 tax ID
[Ref.: #20218]	Escherichia coli strain ATCC 25922 16S ribosomal RNA gene, partial sequence	KC429776	1355	GenBank ENA	562 tax ID	*
[Ref.: #124043]	Escherichia coli ATCC:25922 gene for 16S rRNA, partial sequence.	LC730904	166	GenBank ENA	562 tax ID	*
[Ref.: #124043]	Escherichia coli gene for 16S rRNA, partial sequence, strain: NBRC 15034.	AB680754	1467	GenBank ENA	562 tax ID	*
[Ref.: #20218]	Escherichia coli strain ATCC 25922 16S ribosomal RNA gene, partial sequence	DQ683069	1101	GenBank ENA	562 tax ID	*
[Ref.: #20218]	Escherichia coli strain ATCC 25922 16S ribosomal RNA gene, partial sequence	DQ360844	1532	GenBank ENA	562 tax ID	*
[Ref.: #20218]	Escherichia coli partial 16S rRNA gene, strain ATCC 25922	FM207087	392	GenBank ENA	562 tax ID	*
[Ref.: #20218]	E.coli (ATCC 25922) gene for 16S rRNA	X80724	1452	GenBank ENA	562 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Escherichia coli ATCC 25922	GCA_004151095	contig	GenBank ENA	1322345 tax ID
[Ref.: #67770]	Escherichia coli ATCC 25922	GCA_007844355	scaffold	GenBank ENA	562 tax ID
[Ref.: #66792]	Escherichia coli ATCC 25922	GCA_000743255	complete	GenBank ENA	1322345 tax ID	*
[Ref.: #66792]	Escherichia coli ATCC 25922	GCA_028596225	complete	GenBank ENA	562 tax ID	*
[Ref.: #66792]	Escherichia coli ATCC 25922	GCA_015163465	contig	GenBank ENA	562 tax ID	*
[Ref.: #66792]	Escherichia coli ATCC 25922-1	GCA_007844345	scaffold	GenBank ENA	562 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Escherichia coli ATCC 25922 ATCC 25922 NCBI: GCA_000743255.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	99.75	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	95.623	no
[Ref.: #125438]	aerobic	aerobicⓘ	no	68.005	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	88.948	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	98.5	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	75.93	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Escherichia coli ATCC 25922 NCBI: GCA_017357505
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	88.8
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	81
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	60.8
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	81.4

Availability in culture collections External links

[Ref.: #552]

Culture collection no.

DSM 1103, ATCC 25922, NCIB 12210, WDCM 00013, CCUG 17620, CCUG 7736, CCUG 21456, JCM 5491, CCM 3954, CIP 76.24, IFO 15034, KCTC 1682, LMG 8223, NBRC 15034, NCIMB 12110

[Ref.: #73917]

SI-ID 2805

References

#552	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1103

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41789 ; Curators of the CIP;
#46634 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 17620

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#73917

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID2805.1 )

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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Search for species Escherichia coli in external resources:
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Escherichia coli ATCC 25922 ATCC 25922

Escherichia coli ATCC 25922

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