Escherichia coli K12 DH5alpha is a bacterium of the family Enterobacteriaceae.
genome sequence Bacteria
SI-ID 19187
| @ref 20215 |
Last LPSN update
2026-02-09 11:18:46 |
| Domain Bacteria |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Enterobacterales |
| Family Enterobacteriaceae |
| Genus Escherichia |
| Species Escherichia coli |
| Full scientific name Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980) |
| Synonyms (1) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 2879 | LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) | Medium recipe at MediaDive  | Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | |
|---|---|---|---|---|
| 2879 | positive | growth | 37 |
| @ref | Oxygen tolerance | Confidence | |
|---|---|---|---|
| 125439 | aerobe | 92.1 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68368 | 27613 ChEBI | amygdalin | - | fermentation | from API 20E |
| 68368 | 29016 ChEBI | arginine | - | hydrolysis | from API 20E |
| 68368 | 16947 ChEBI | citrate | - | assimilation | from API 20E |
| 68368 | 17634 ChEBI | D-glucose | + | fermentation | from API 20E |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 68368 | 30849 ChEBI | L-arabinose | + | fermentation | from API 20E |
| 68368 | 62345 ChEBI | L-rhamnose | + | fermentation | from API 20E |
| 68368 | 17268 ChEBI | myo-inositol | - | fermentation | from API 20E |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 68368 | 30911 ChEBI | sorbitol | + | fermentation | from API 20E |
| 68368 | 17992 ChEBI | sucrose | - | fermentation | from API 20E |
| 68368 | 27897 ChEBI | tryptophan | + | energy source | from API 20E |
| 68368 | 16199 ChEBI | urea | - | hydrolysis | from API 20E |
Phages| @ref | Name | Strain number | |
|---|---|---|---|
| 124042 | Escherichia phage vB_EcoP-PMBT57 | DSM 6897 | |
| 124042 | Escherichia phage vB_EcoM-Bhz63 | DSM 6897 |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM289947v1 assembly for Escherichia coli DH5alpha | complete | GCA_002899475   | 562.28198  | 8011498891  | 562 | 99.47 | |
| 66792 | ASM284822v1 assembly for Escherichia coli DH5alpha | complete | GCA_002848225 | 562.114653 | 8011494152 | 562 | 96.82 | |
| 66792 | ASM964849v1 assembly for Escherichia coli DH5alpha | complete | GCA_009648495 | 562.52708 | 562 | 95.01 | | |
| 66792 | ASM635222v1 assembly for Escherichia coli DH5alpha | contig | GCA_006352225 | 562.50415 | 562 | |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Organoarsonate- and Dimethylarsinate-Functionalized Hexamolybdates(V): A Multifaceted Study on Synthesis, Structural Dynamics, and Antibacterial Properties. | Siby V, Pal A, Sarkar A, Bassil BS, Immoor A, Ziemah J, Holscher J, Kiss L, Silvestru C, Ullrich MS, Kuhnert N, Kortz U. | Inorg Chem | 10.1021/acs.inorgchem.5c02852 | 2025 | | |
| Application of a replicative targetable vector system for difficult-to-manipulate streptomycetes. | Gomez-Escribano JP, Zimmermann A, Xia SN, Doppner M, Moschny J, Hughes CC, Mast Y. | Appl Microbiol Biotechnol | 10.1007/s00253-025-13477-3 | 2025 | | |
| Pathogenicity | Aetokthonotoxin, the Causative Agent of Vacuolar Myelinopathy, Uncouples Oxidative Phosphorylation due to Protonophore Activity. | Rebhahn VIC, Saoud M, Winterhalter M, Schanbacher F, Jobst M, Ruiz R, Sonntag A, Kollatz J, Sprengel R, Donovan SF, Del Favero G, Rennert R, Niedermeyer THJ. | Chem Res Toxicol | 10.1021/acs.chemrestox.5c00147 | 2025 | |
| Deciphering the Crucial Roles of the Quorum-Sensing Transcription Factor SdiA in NADPH Metabolism and (S)-Equol Production in Escherichia coli Nissle 1917. | Wang Z, Dai Y, Azi F, Dong M, Xia X. | Antioxidants (Basel) | 10.3390/antiox13030259 | 2024 | | |
| Photothermally Controlled Drug Release of Poly(d,l-lactide) Nanofibers Loaded with Indocyanine Green and Curcumin for Efficient Antimicrobial Photodynamic Therapy. | Gutberlet B, Preis E, Roschenko V, Bakowsky U. | Pharmaceutics | 10.3390/pharmaceutics15020327 | 2023 | | |
| Microfluidic One-Pot Digital Droplet FISH Using LNA/DNA Molecular Beacons for Bacteria Detection and Absolute Quantification. | Kao YT, Calabrese S, Borst N, Lehnert M, Lai YK, Schlenker F, Juelg P, Zengerle R, Garstecki P, von Stetten F. | Biosensors (Basel) | 10.3390/bios12040237 | 2022 | | |
| Natural Occurrence of Escherichia coli-Infecting Bacteriophages in Clinical Samples. | Pacifico C, Hilbert M, Sofka D, Dinhopl N, Pap IJ, Aspock C, Carrico JA, Hilbert F. | Front Microbiol | 10.3389/fmicb.2019.02484 | 2019 | | |
| Parietin Cyclodextrin-Inclusion Complex as an Effective Formulation for Bacterial Photoinactivation. | Ayoub AM, Gutberlet B, Preis E, Abdelsalam AM, Abu Dayyih A, Abdelkader A, Balash A, Schafer J, Bakowsky U. | Pharmaceutics | 10.3390/pharmaceutics14020357 | 2022 | | |
| Conformational exchange divergence along the evolutionary pathway of eosinophil-associated ribonucleases. | Bernard DN, Narayanan C, Hempel T, Bafna K, Bhojane PP, Letourneau M, Howell EE, Agarwal PK, Doucet N. | Structure | 10.1016/j.str.2022.12.011 | 2023 | | |
| Reliability of species detection in 16S microbiome analysis: Comparison of five widely used pipelines and recommendations for a more standardized approach. | Hiergeist A, Ruelle J, Emler S, Gessner A. | PLoS One | 10.1371/journal.pone.0280870 | 2023 | | |
| In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli. | Korf IHE, Kittler S, Bierbrodt A, Mengden R, Rohde C, Rohde M, Kroj A, Lehnherr T, Fruth A, Flieger A, Lehnherr H, Wittmann J. | Viruses | 10.3390/v12121470 | 2020 | | |
| Characterization of Bacteria and Inducible Phages in an Intensive Care Unit. | Pacifico C, Hilbert M, Sofka D, Dinhopl N, Pap IJ, Aspock C, Hilbert F. | J Clin Med | 10.3390/jcm8091433 | 2019 | | |
| Enzymology | Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling. | Jian C, Luukkonen P, Yki-Jarvinen H, Salonen A, Korpela K. | PLoS One | 10.1371/journal.pone.0227285 | 2020 | |
| Prebiotic Potential of a New Sweetener Based on Galactooligosaccharides and Modified Mogrosides. | Munoz-Labrador A, Lebron-Aguilar R, Quintanilla-Lopez JE, Galindo-Iranzo P, Azcarate SM, Kolida S, Kachrimanidou V, Garcia-Canas V, Methven L, Rastall RA, Moreno FJ, Hernandez-Hernandez O. | J Agric Food Chem | 10.1021/acs.jafc.2c01363 | 2022 | | |
| Pathogenicity | Evaluation and Optimization of Protein Extraction From E. coli by Electroporation. | Haberl Meglic S, Janez N, Peterka M, Flisar K, Kotnik T, Miklavcic D. | Front Bioeng Biotechnol | 10.3389/fbioe.2020.543187 | 2020 | |
| Probing and Manipulating the Lateral Pressure Profile in Lipid Bilayers Using Membrane-Active Peptides-A Solid-State 19F NMR Study. | Grage SL, Afonin S, Ieronimo M, Berditsch M, Wadhwani P, Ulrich AS. | Int J Mol Sci | 10.3390/ijms23094544 | 2022 | | |
| Biotechnology | Boosting Escherichia coli's heterologous production rate of ectoines by exploiting the non-halophilic gene cluster from Acidiphilium cryptum. | Bethlehem L, Moritz KD. | Extremophiles | 10.1007/s00792-020-01188-8 | 2020 | |
| Promoters from the itaconate cluster of Ustilago maydis are induced by nitrogen depletion. | Zambanini T, Hartmann SK, Schmitz LM, Buttner L, Hosseinpour Tehrani H, Geiser E, Beudels M, Venc D, Wandrey G, Buchs J, Schwarzlander M, Blank LM, Wierckx N. | Fungal Biol Biotechnol | 10.1186/s40694-017-0040-3 | 2017 | | |
| Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay. | Brunauer A, Verboket RD, Kainz DM, von Stetten F, Fruh SM. | Biosensors (Basel) | 10.3390/bios11030074 | 2021 | | |
| Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation. | Kolek J, Sedlar K, Provaznik I, Patakova P. | Biotechnol Biofuels | 10.1186/s13068-016-0436-y | 2016 | | |
| Chip calorimetry for the monitoring of whole cell biotransformation. | Maskow T, Lerchner J, Peitzsch M, Harms H, Wolf G. | J Biotechnol | 10.1016/j.jbiotec.2005.10.008 | 2006 | | |
| Metabolism | Identification of an endo-1,4-beta-xylanase of Ustilago maydis. | Geiser E, Wierckx N, Zimmermann M, Blank LM. | BMC Biotechnol | 10.1186/1472-6750-13-59 | 2013 | |
| Metabolism | Novel insights into biosynthesis and uptake of rhamnolipids and their precursors. | Wittgens A, Kovacic F, Muller MM, Gerlitzki M, Santiago-Schubel B, Hofmann D, Tiso T, Blank LM, Henkel M, Hausmann R, Syldatk C, Wilhelm S, Rosenau F. | Appl Microbiol Biotechnol | 10.1007/s00253-016-8041-3 | 2017 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive4639.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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