Name: Escherichia coli BfR/NRL-AR-E476
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 4923

Type strain:

Species: Escherichia coli

Strain Designation: BfR/NRL-AR-E476, NRL-EcRL284/06, NRL-Salm06E00094

Culture col. no.: DSM 22665

Strain history: <- B. Guerra Román, Natl. Reference Lab. Antimicrobial Resistance (NRL-AR), Federal Inst. Risk Assessment (BfR), Berlin, Germany; BfR/NRL-AR-E476 <- Natl. Salmonella Reference Lab. (NRL-Salm), Federal Inst. Risk Assessment (BfR), Berlin, Germany; NRL-Salm06E00094 <- L. Beutin, Natl. Reference Lab. Escherichia coli (NRL-EC), Federal Inst. Risk Assessment (BfR), Berlin, Germany; NRL-EcRL284/06

NCBI tax ID(s): 562 (species)

Special Collections

DSMZ

Other strains from Escherichia coli

Section

Escherichia coli BfR/NRL-AR-E476 is a human pathogen that has multiple antibiotic resistances and was isolated from milk .

antibiotic resistance
human pathogen
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Escherichia*
	Species	**Escherichia coli**
	Full Scientific Name (LPSN)	Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

Synonym

"Bacillus coli"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16455]

Culture medium

LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)

[Ref.: #16455]

Culture medium growth

[Ref.: #16455]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16455]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16455]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Reference

[Ref.: #16455]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
resistant0
resistant0
resistant0
resistant0
8
resistant0
resistant0
20
24
36
resistant0
30
22
22
resistant0
10
resistant0
34
40
14
28
24
resistant0
14-16
32-34
20
10
18
resistant0
36
32
resistant0
resistant0
resistant0
resistant0
26

Only first 15 entries are displayed. Click here to see all.

[Ref.: #16455]

Name of produced compound

CTX-M1 beta lactamase

[Ref.: #16455]

Observation

ESBL

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #16455]	17594	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	+	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #16455]	17592	+	-	+	+	-	-	-	-	+	-	-	+	+	-	+	+	+	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16455]	Sample type/isolated from	milk (cow with mastitis)
[Ref.: #16455]	Geographic location (country and/or sea, region)	Saxony
[Ref.: #16455]	Country	Germany
[Ref.: #16455]	Country ISO 3 Code	DEU
[Ref.: #16455]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16455]	Pathogenicity (human)	yes
[Ref.: #16455]	Pathogenicity (animal)	yes
[Ref.: #16455]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Availability in culture collections External links

[Ref.: #16455]

Culture collection no.

DSM 22665

References

#16455

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22665

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#68368

Automatically annotated from API 20E .

* These data were automatically processed and therefore are not curated

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