Escherichia coli K12 HMS174 is a mesophilic prokaryote of the family Enterobacteriaceae.
mesophilic genome sequence| @ref 20215 |
|
|
| Domain Bacteria |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Enterobacterales |
| Family Enterobacteriaceae |
| Genus Escherichia |
| Species Escherichia coli |
| Full scientific name Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980) |
| Synonyms (1) |
| @ref | Gram stain | Confidence | |
|---|---|---|---|
| 125438 | negative | 99.75 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 2388 | LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) | Medium recipe at MediaDive | Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 2388 | positive | growth | 37 | mesophilic |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68368 | 27613 ChEBI | amygdalin | - | fermentation | from API 20E |
| 68368 | 29016 ChEBI | arginine | - | hydrolysis | from API 20E |
| 68368 | 16947 ChEBI | citrate | - | assimilation | from API 20E |
| 68368 | 17634 ChEBI | D-glucose | + | fermentation | from API 20E |
| 68368 | 16899 ChEBI | D-mannitol | + | fermentation | from API 20E |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 68368 | 30849 ChEBI | L-arabinose | + | fermentation | from API 20E |
| 68368 | 62345 ChEBI | L-rhamnose | + | fermentation | from API 20E |
| 68368 | 25094 ChEBI | lysine | + | degradation | from API 20E |
| 68368 | 28053 ChEBI | melibiose | + | fermentation | from API 20E |
| 68368 | 17268 ChEBI | myo-inositol | - | fermentation | from API 20E |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 68368 | 30911 ChEBI | sorbitol | + | fermentation | from API 20E |
| 68368 | 17992 ChEBI | sucrose | - | fermentation | from API 20E |
| 68368 | 27897 ChEBI | tryptophan | + | energy source | from API 20E |
| 68368 | 16199 ChEBI | urea | - | hydrolysis | from API 20E |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68368 | arginine dihydrolase | - | 3.5.3.6 | from API 20E |
| 68368 | beta-galactosidase | + | 3.2.1.23 | from API 20E |
| 68368 | gelatinase | - | from API 20E | |
| 68368 | lysine decarboxylase | + | 4.1.1.18 | from API 20E |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 68368 | urease | - | 3.5.1.5 | from API 20E |
| @ref | Description | Assembly level | INSDC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|
| 124043 | EcHMS174Chr assembly for Escherichia coli K-12 substr. HMS174 | complete | 562 | 99.12 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | no | 99.75 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 95.80 | no |
| 125438 | spore-forming | spore-formingⓘ | no | 87.95 | no |
| 125438 | aerobic | aerobicⓘ | no | 59.86 | no |
| 125438 | thermophilic | thermophileⓘ | no | 98.50 | yes |
| 125438 | flagellated | motile2+ⓘ | yes | 75.36 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Enzymology | Antimicrobial peptide plectasin recombinantly produced in Escherichia coli disintegrates cell walls of gram-positive bacteria, as proven by transmission electron and atomic force microscopy. | Muller M, Mayrhofer S, Sudjarwo WAA, Gibisch M, Tauer C, Berger E, Brocard C, Toca-Herrera JL, Striedner G, Hahn R, Cserjan-Puschmann M. | J Bacteriol | 10.1128/jb.00456-24 | 2025 | |
| Finished Genome Sequence of Escherichia coli K-12 Strain HMS174 (ATCC 47011). | Mairhofer J, Krempl PM, Thallinger GG, Striedner G. | Genome Announc | 10.1128/genomea.00975-14 | 2014 | ||
| Metabolism | Effect of pH on intracellular accumulation of trace concentrations of Hg(II) in Escherichia coli under anaerobic conditions, as measured using a mer-lux bioreporter. | Golding GR, Sparling R, Kelly CA. | Appl Environ Microbiol | 10.1128/aem.00717-07 | 2008 | |
| Metabolism | Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain. | Slater S, Gallaher T, Dennis D. | Appl Environ Microbiol | 10.1128/aem.58.4.1089-1094.1992 | 1992 | |
| Mocr: a novel fusion tag for enhancing solubility that is compatible with structural biology applications. | DelProposto J, Majmudar CY, Smith JL, Brown WC. | Protein Expr Purif | 10.1016/j.pep.2008.08.011 | 2009 |
| #2388 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5932 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #68368 | Automatically annotated from API 20E . |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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