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Name and taxonomic classification
Domain name (taxonomic rank)Domain
Phylum name (taxonomic rank)Phylum
Class name (taxonomic rank).Class
name of order. taxonomic rank.Order
Family name (taxonomic rank)Family
Genus name (taxonomic rank)Genus
Second part of species nameSpecies epithet
Third part of species name, where applicableSubspecies epithet
Species name, which includes genus, species and subspecies epithet (taxonomic rank)Species name (e.g. Escherichia coli )
Species name, author(s) and year of the valid publication of the species name. Full Scientific Name
Name of the strain as given in publications or public databasesStrain Designation
variations of a strain, e.g. serotype, biovar or isotypeVariant
Is this strain a type strain.Type strain
Species name, author(s) and year of the valid publication of the species name. Full Scientific Name (PNU)
Morphology and physiology
Gram staining Gram stain
Length of the cell.Cell length
Width of the cellCell width
Description of the cell shape.Cell shape
alpha-, beta or gamma-hemolysisHemolysis
Length of the colony on distinct medium and temperature after a certain time of cultivation.Colony size
Incubation period in context with the description of colony morphologyIncubation period (in days)
Ability of spore formation.Ability of spore formation
Ability of forming multicellular complexes (aggregations). Multicellular complex forming ability
Name of produced compound (e.g. name of a secondary metabolite).Name of produced compound
Kind of salt related to halophily information usually NaCl.Salt
Tested salt concentration (usually in %)Salt conc.
e.g. %(w/v), %salt concentration unit
Kind of relation for a tested salt concentrationTested relation
Ability of salt tolerance.Testresult
To save space, a special system has been developed to characterize the different murein types. This key enables the user to link the abbreviations to the system proposed for the characterization and representation of peptidoglycan types proposed by Schleifer and Kandler 1972. An extension by an apostrophe e.g. A3 alpha' denotes that the L-Ala residue found normally in position 1 of the peptide subunit is replaced by Gly.Murein short key
Characterization of the different murein types.Murein types
Known attribute or ability of the strain e.g. cellulose decompositon, yeast lysisObservation
Able/ not able of e.g. decomposition/lysisAbility
information depends on applied culture mediumOxygen tolerance
e.g. a substrate, an electron acceptor, a degraded metaboliteMetabolite (utilization)
Ability of metabolite utilization.Utilization activity
Type of applied utilization test.Kind of utilization tested
e.g. a substrate, an electron acceptor, a degraded metaboliteMetabolite (antibiotic)
Concentration in g/LAntibiotic sensitivity conc.
Concentration in g/LAntibiotic intermediate conc.
General relation of an organism to an antibiotic substanceAntibiotic resistance
Concentration in g/LAntibiotic resistance conc.
e.g. a substrate, an electron acceptor, a degraded metaboliteMetabolite (production)
Yes if it is a metabolite productionProduction
Test for acetoin productionVoges-Proskauer-test (Acetoin)
Test for mixed acid fermentation on glucoseMethylred-test (Glucose)
Test for ability to convert tryptophan into indoleIndole test (Indole)
Test for the ability of using citrate as sole carbon source.Citrate test (Citric acid)
enzyme e.g. catalase, urease, alkaline phosphataseEnzyme
Tested activityEnzyme activity
Corresponding EC numberEC number
APIs
Culture and growth conditions
Name of the culture mediumCulture medium
if 1 growth, 0 no growthCulture medium growth
link to medium recipeCulture medium link
Components of the culture medium and recipeCulture medium composition
temperature [°C]Temperature
e.g. maximum, optimum or growth in generalKind of temperature
positive/negative test resultTestresult
psychrophilic (<25°C), mesophilic (25-39°C), thermophilic (40-79°C), hyperthermophilic (>80°C)Temperature range
tested pH valuespH
e.g. maximum, optimum or growth in generalKind of pH
positive/negative test resultTestresult
Isolation, sampling and environmental information
property of the sample the bacterium was isolated from.Sample type/isolated from
The host species where a strain was isolated fromHost species
The geographical origin of the sample as defined by the country or sea name followed by specific region name.Geographic location (country and/or sea, region)
country name: sample material/strain originCountry
continent name: sample material/strain originContinent
Application and interaction
Ability of causing pathological conditions within the referring organismPathogenicity (human)
Ability of causing pathological conditions within the referring organismPathogenicity (animal)
Ability of causing pathological conditions within the referring organismPathogenicity (plant)
Biosafety level defined for the strainBiosafety level
Molecular biology
Known accession numbers of sequence databases can be entered.Sequence accession number
Given title of this accession number in the INSDC sequence database.Sequence accession description
NCBI tax ID Associated which is associated to the refering INSDC accession number.Associated NCBI tax ID
Sequence typeSequence type
Guanine-Cytosine content in mol % GC-content
Strain availability
Accession number, i.e. the unique number given by a public culture collection or bioresource center to the strain, where applicable more than one per strain (e.g. DSM 2324, ATCC 6438, NCIB 2224)Culture collection no.
Strain passport(s) associated to the strain at StrainInfo.net and its number(s) given by a public culture collection or bioresource center, where applicable more than one per strainAssociated Passport(s) in StrainInfo
Antibiotic susceptibility testing
6 µg (disc)Penicillin G		Inhibition zone diameter in mm
5 µg (disc)Oxacillin		Inhibition zone diameter in mm
10 µg (disc)Ampicillin		Inhibition zone diameter in mm
75 µg (disc)Ticarcillin		Inhibition zone diameter in mm
30 µg (disc)Mezlocillin		Inhibition zone diameter in mm
30 µg (disc)Cefalotin		Inhibition zone diameter in mm
30 µg (disc)Cefazolin		Inhibition zone diameter in mm
30 µg (disc)Cefotaxime		Inhibition zone diameter in mm
30 µg (disc)Aztreonam		Inhibition zone diameter in mm
10 µg (disc)Imipenem		Inhibition zone diameter in mm
30 µg (disc)Tetracycline		Inhibition zone diameter in mm
30 µg (disc)Chloramphenicol		Inhibition zone diameter in mm
10 µg (disc)Gentamycin		Inhibition zone diameter in mm
30 µg (disc)Amikacin		Inhibition zone diameter in mm
30 µg (disc)Vancomycin		Inhibition zone diameter in mm
15 µg (disc)Erythromycin		Inhibition zone diameter in mm
15 µg (disc)Lincomycin		Inhibition zone diameter in mm
5 µg (disc)Ofloxacin		Inhibition zone diameter in mm
10 µg (disc)Norfloxacin		Inhibition zone diameter in mm
10 µg (disc)Colistin		Inhibition zone diameter in mm
20 µg (disc)Pipemidic acid		Inhibition zone diameter in mm
100 µg (disc)Nitrofurantoin		Inhibition zone diameter in mm
10 UnitBacitracin		Inhibition zone diameter in mm
300 UnitPolymyxin B		Inhibition zone diameter in mm
30 µg (disc)Kanamycin		Inhibition zone diameter in mm
30 µg (disc)Neomycin		Inhibition zone diameter in mm
30 µg (disc)Doxycycline		Inhibition zone diameter in mm
30 µg (disc)Ceftriaxone		Inhibition zone diameter in mm
10 µg (disc)Clindamycin		Inhibition zone diameter in mm
50 µg (disc)Fosfomycin		Inhibition zone diameter in mm
5 µg (disc)Moxifloxacin		Inhibition zone diameter in mm
10 µg (disc)Linezolid		Inhibition zone diameter in mm
100 UnitNystatin		Inhibition zone diameter in mm
15 µg (disc)Quinupristin/Dalfopristin		Inhibition zone diameter in mm
30 µg (disc)Teicoplanin		Inhibition zone diameter in mm
30/10 µg (disc)Piperacillin/Tazobactam		Inhibition zone diameter in mm
Fatty acid profile
e.g. C12:0Fatty acid
Estimated chain length if availableECL
percentagePercentage
e.g. whole cell analysisType of FA analysis
Incubation mediumIncubation medium
cultivation on agar or in liquid mediumAgar/liquid
Incubation temperature (°C)Incubation temperature (°C)
Incubation time in days on the respective mediumIncubation time (d)
Incubation pH on the respective mediumIncubation pH
Incubation oxygen conditionIncubation oxygen condition
Additional treatments/conditionsTreatment/condition
e.g. Sherlock X.XSoftware version
e.g. TSBA 6Library/peak naming table
e.g. MIS MIDISystem
e.g. Agilent XXXXAInstrument
e.g. Sasser 1990Method/protocol
e.g. 0.1,0.5,1Cutoff value
Reference
Title of referenceTitle
List of author(s)Authors
Journal Name of ReferenceJournal (Abbreviation)
Year of publication of the referenceYear
Reference identified via a Digital Object Identifier (DOI)DOI (Digital Object Identifier)
ID_reference

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