Acetivibrio thermocellus (DSM 1237, ATCC 27405, JCM 9322)

Name: Acetivibrio thermocellus (DSM 1237, ATCC 27405, JCM 9322)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2729

Type strain

Culture col. no. DSM 1237 ATCC 27405 JCM 9322 NCIMB 10682 CECT 593 9 more

NCBI tax ID(s) 203119 1515

Special Collections DSMZ JCM Literature strains JMRC

History DSMZ <- NCIB <- G. Hobbs, Torry Research Station, Aberdeen, U.K. <- R. H. McBee, Montana State Univ., Bozeman, USA JCM <-- E. Uematsu <-- ATCC 27405 <-- L. DS. Smith VPI 7372 <-- J. Alexander <-- R. McBee.

Links

Acetivibrio thermocellus DSM 1237 is an anaerobe, spore-forming bacterium that was isolated from Sewage digestor sludge.

spore-forming anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 10383

LMG 10435,ATCC 27405,NCIMB 10682,NCIB 10682,JCM 9322,IAM13660,CECT 593,CCRC 14411,VTT E-81137,KCTC 1672,JCM 12338,BCRC 14411,NRRL B-4536,NBRC 103400,DSM 1237

@ref 20215

Last LPSN update 2026-05-29 08:25:32

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Oscillospiraceae

Genus Acetivibrio

Species Acetivibrio thermocellus

Full scientific name Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019

Synonyms (3)

Clostridium thermocellum
"Ruminiclostridium thermocellum"
Hungateiclostridium thermocellum

BacDive ID	Other strains from **Acetivibrio thermocellus** (6)	Type strain
2728	A. thermocellus LQ8, DSM 1313
2730	A. thermocellus LQR1, DSM 2360, JCM 9323, ATCC 35609
2731	A. thermocellus JW 20, DSM 4150, ATCC 31549
22965	A. thermocellus YS, DSM 25991
22966	A. thermocellus AD2, DSM 25992
174402	A. thermocellus DSM 29190, BC 1

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
545	ACETIVIBRIO MEDIUM (DSMZ Medium 122)		Medium recipe at MediaDive	Name: ACETIVIBRIO MEDIUM (DSMZ Medium 122) Composition: Cellulose 10.0 g/l (optional) Na2-ß-glycerophosphate x 5 H2O 6.0 g/l K2HPO4 5.5 g/l Cellobiose 5.0 g/l Yeast extract 4.5 g/l MgCl2 x 6 H2O 2.6 g/l KH2PO4 1.43 g/l (NH4)2SO4 1.3 g/l L-Glutathione 0.25 g/l CaCl2 x 2 H2O 0.13 g/l FeSO4 x 7 H2O 0.0011 g/l Sodium resazurin 0.0005 g/l Distilled water
545	RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM (DSMZ Medium 520)		Medium recipe at MediaDive	Name: RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM (DSMZ Medium 520) Composition: Cellulose 9.97009 g/l (optional) Cellobiose 5.98205 g/l K2HPO4 x 3 H2O 2.89133 g/l Yeast extract 1.99402 g/l Na2CO3 1.49551 g/l KH2PO4 1.49551 g/l (NH4)2SO4 1.29611 g/l L-Cysteine HCl x H2O 0.498504 g/l MgCl2 x 6 H2O 0.199402 g/l CaCl2 x 2 H2O 0.0747757 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l FeSO4 x 7 H2O 0.00124626 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
545	positive	growth	55
22909	positive	growth	60
67770	positive	growth	55

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
545	anaerobe
125438	anaerobe	95.236
125439	anaerobe	98.406

Spore formation

22909

Spore formationyes

Compound production

@ref	Compound
545	restriction endonuclease CthI
545	restriction endonuclease CthII
545	restriction endonucleases CthI, CthII

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
22909	62968 ChEBI	cellulose	+	builds acid from
22909	28757 ChEBI	fructose	-	builds acid from
22909	17234 ChEBI	glucose	-	builds acid from
22909	29864 ChEBI	mannitol	-	builds acid from
22909	28017 ChEBI	starch	-	builds acid from
22909	17992 ChEBI	sucrose	-	builds acid from

Enzymes

@ref	Value	Ec
67770	cellulase	3.2.1.4

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cellulose degradation	100	5 of 5
66794	glycogen metabolism	100	5 of 5
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	L-lactaldehyde degradation	100	3 of 3
66794	biotin biosynthesis	100	4 of 4
66794	suberin monomers biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	folate polyglutamylation	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	starch degradation	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	isoleucine metabolism	87.5	7 of 8
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	vitamin B1 metabolism	84.62	11 of 13
66794	pyrimidine metabolism	84.44	38 of 45
66794	threonine metabolism	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycine betaine biosynthesis	80	4 of 5
66794	heme metabolism	78.57	11 of 14
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	valine metabolism	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	sulfopterin metabolism	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	photosynthesis	71.43	10 of 14
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glutamate and glutamine metabolism	67.86	19 of 28
66794	octane oxidation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	purine metabolism	65.96	62 of 94
66794	glycolysis	64.71	11 of 17
66794	d-xylose degradation	63.64	7 of 11
66794	oxidative phosphorylation	62.64	57 of 91
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	methionine metabolism	61.54	16 of 26
66794	hydrogen production	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	alanine metabolism	58.62	17 of 29
66794	histidine metabolism	58.62	17 of 29
66794	arginine metabolism	58.33	14 of 24
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	glutathione metabolism	57.14	8 of 14
66794	propanol degradation	57.14	4 of 7
66794	degradation of sugar alcohols	56.25	9 of 16
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	urea cycle	53.85	7 of 13
66794	lipid metabolism	51.61	16 of 31
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	ethanol fermentation	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	adipate degradation	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	tryptophan metabolism	50	19 of 38
66794	glycine metabolism	50	5 of 10
66794	propionate fermentation	50	5 of 10
66794	ketogluconate metabolism	50	4 of 8
66794	butanoate fermentation	50	2 of 4
66794	cysteine metabolism	50	9 of 18
66794	leucine metabolism	46.15	6 of 13
66794	sulfate reduction	46.15	6 of 13
66794	proline metabolism	45.45	5 of 11
66794	lysine metabolism	45.24	19 of 42
66794	nitrate assimilation	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	citric acid cycle	42.86	6 of 14
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	vitamin B12 metabolism	41.18	14 of 34
66794	Entner Doudoroff pathway	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	metabolism of disaccharids	36.36	4 of 11
66794	vitamin B6 metabolism	36.36	4 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	36.36	4 of 11
66794	degradation of pentoses	35.71	10 of 28
66794	tyrosine metabolism	35.71	5 of 14
66794	polyamine pathway	34.78	8 of 23
66794	lipid A biosynthesis	33.33	3 of 9
66794	IAA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	molybdenum cofactor biosynthesis	33.33	3 of 9
66794	methane metabolism	33.33	1 of 3
66794	ascorbate metabolism	31.82	7 of 22
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	coenzyme M biosynthesis	30	3 of 10
66794	arachidonic acid metabolism	27.78	5 of 18
66794	pentose phosphate pathway	27.27	3 of 11
66794	toluene degradation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	phenol degradation	25	5 of 20
66794	catecholamine biosynthesis	25	1 of 4
66794	carnitine metabolism	25	2 of 8

Isolation, sampling and environmental information

Isolation

67770

Sample typeSewage digestor sludge

Taxonmaps

69479

Global distribution of 16S sequence L09173 (>99% sequence identity) for Acetivibrio thermocellus subclade from Microbeatlas

Aquatic Samples	1005
Soil Samples	2753
Animal Samples	2787
Plant Samples	816
Total Samples	7361

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
545	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1586v1 assembly for Acetivibrio thermocellus ATCC 27405	complete	GCA_000015865	203119.11	640069309	203119	97.58

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Clostridium thermocellum (DSM 1237) 16S ribosomal RNA (16S rRNA) gene	L09173	1509		1515
20218	Clostridium thermocellum gene for 16S ribosomal RNA, partial sequence, strain: JCM 9322	AB558166	1489		1515

GC content

@ref	GC-content (mol%)	Method
22909	38
67770	38	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1586v1 assembly for Acetivibrio thermocellus ATCC 27405
BV-BRC: 203119.11

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.41	no
125439	gram_stain	BacteriaNetⓘ	variable	66.46	no
125439	motility	BacteriaNetⓘ	yes	73.52	no
125439	spore_formation	BacteriaNetⓘ	yes	73.37	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acetivibrio thermocellus ATCC 27405 ATCC 27405
NCBI: GCA_000015865.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	59.25	no
125438	anaerobic	anaerobicⓘ	yes	95.24	yes
125438	aerobic	aerobicⓘ	no	98.02	yes
125438	spore-forming	spore-formingⓘ	yes	84.17	yes
125438	thermophilic	thermophileⓘ	yes	71.17	yes
125438	flagellated	motile2+ⓘ	yes	78.71	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases by Bacillus licheniformis and Bacillus velezensis.	Arsov A, Petrov K, Petrova P.	Molecules	10.3390/molecules26185625	2021
Enzymology	Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles.	Daley SR, Gallanosa PM, Sparling R.	Biotechnol Biofuels Bioprod	10.1186/s13068-023-02362-8	2023
	Construction of an efficient thermostable cellulolytic chimera of beta-glucosidase and beta-1,4-endoglucanase from Acetivibrio thermocellus ATCC 27405 and its application in lignocellulose saccharification.	Kanwar A, Dhillon A, Goyal A.	Int J Biol Macromol	10.1016/j.ijbiomac.2025.146785	2025
	Construction of an efficient thermostable cellulolytic chimera of beta-glucosidase and beta-1,4-endoglucanase from Acetivibrio thermocellus ATCC 27405 and its application in lignocellulose saccharification	Kanwar A, Dhillon A, Goyal A.	International Journal of Biological Macromolecules.		2025
	Structure elucidation of a multi-modular recombinant endoglucanase, AtGH9C-CBM3A-CBM3B from Acetivibrio thermocellus ATCC 27405 and its substrate binding analysis.	Mandal A, Ahmed J, Singh S, Goyal A.	Int J Biol Macromol	10.1016/j.ijbiomac.2024.133212	2024
	Role of carbohydrate binding modules, CBM3A and CBM3B in stability and catalysis by a beta-1,4 endoglucanase, AtGH9C-CBM3A-CBM3B from Acetivibrio thermocellus ATCC 27405.	Mandal A, Thakur A, Goyal A.	Int J Biol Macromol	10.1016/j.ijbiomac.2023.125164	2023
	Cellulolytic and hemicellulolytic capacity of Acetivibrio clariflavus.	Suchova K, Puchart V.	Appl Microbiol Biotechnol	10.1007/s00253-025-13471-9	2025
Enzymology	Novel Function of CtXyn5A from Acetivibrio thermocellus: Dual Arabinoxylanase and Feruloyl Esterase Activity in the Same Active Site.	Schmitz E, Leontakianakou S, Adlercreutz P, Nordberg Karlsson E, Linares-Pasten JA.	Chembiochem	10.1002/cbic.202200667	2023
	Non-Catalytic Domains of Glycoside Hydrolase Family 5 from Paenibacillus curdlanolyticus are Important for Promoting Multifunctional Enzyme Activities and Degradation of Agricultural Residues.	Fatmawati NV, Singkhala A, Ketbot P, Baramee S, Waeonukul R, Tachaapaikoon C, Uke A, Kosugi A, Ratanakhanokchai K, Pason P.	J Microbiol Biotechnol	10.4014/jmb.2501.01046	2025
	Identification and characterization of a novel, low-temperature-active GH8 endo-beta-1,4-glucanase exhibiting broad pH stability from Antarctic Glacieibacterium sp. PAMC 29367.	Kim DY, Lee YM, Lee JS, Kim H, Chung CW.	Front Microbiol	10.3389/fmicb.2025.1682092	2025
	Effective semi-fed-batch saccharification with high lignocellulose loading using co-culture of Clostridium thermocellum and Thermobrachium celere strain A9.	Nhim S, Baramee S, Tachaapaikoon C, Pason P, Ratanakhanokchai K, Uke A, Ceballos RM, Kosugi A, Waeonukul R.	Front Microbiol	10.3389/fmicb.2024.1519060	2024
Genetics	Genomic analysis and chitinase characterization of Vibrio harveyi WXL538: insight into its adaptation to the marine environment.	Ran L, Wang X, He X, Guo R, Wu Y, Zhang P, Zhang XH.	Front Microbiol	10.3389/fmicb.2023.1121720	2023
Biotechnology	Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases.	Li A, Benkoulouche M, Ladeveze S, Durand J, Cioci G, Laville E, Potocki-Veronese G.	Int J Mol Sci	10.3390/ijms23063043	2022
Enzymology	Structure and function of microbial alpha-l-fucosidases: a mini review.	Wu H, Owen CD, Juge N.	Essays Biochem	10.1042/ebc20220158	2023
Genetics	A genomic analysis reveals the diversity of cellulosome displaying bacteria.	Minor CM, Takayesu A, Ha SM, Salwinski L, Sawaya MR, Pellegrini M, Clubb RT.	Front Microbiol	10.3389/fmicb.2024.1473396	2024
Phylogeny	Introduction of Cellulolytic Bacterium Bacillus velezensis Z2.6 and Its Cellulase Production Optimization.	Cai Z, Wang Y, You Y, Yang N, Lu S, Xue J, Xing X, Sha S, Zhao L.	Microorganisms	10.3390/microorganisms12050979	2024
Enzymology	Ethanol tolerance in engineered strains of Clostridium thermocellum.	Olson DG, Maloney MI, Lanahan AA, Cervenka ND, Xia Y, Pech-Canul A, Hon S, Tian L, Ziegler SJ, Bomble YJ, Lynd LR.	Biotechnol Biofuels Bioprod	10.1186/s13068-023-02379-z	2023
	The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.	Mahoney BJ, Takayesu A, Zhou A, Cascio D, Clubb RT.	Proteins	10.1002/prot.26326	2022
Metabolism	Laboratory Evolution and Reverse Engineering of Clostridium thermocellum for Growth on Glucose and Fructose.	Yayo J, Kuil T, Olson DG, Lynd LR, Holwerda EK, van Maris AJA.	Appl Environ Microbiol	10.1128/aem.03017-20	2021
	Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum.	Yan F, Dong S, Liu YJ, Yao X, Chen C, Xiao Y, Bayer EA, Shoham Y, You C, Cui Q, Feng Y.	mBio	10.1128/mbio.01476-22	2022
	Biological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9.	Nhim S, Waeonukul R, Uke A, Baramee S, Ratanakhanokchai K, Tachaapaikoon C, Pason P, Liu YJ, Kosugi A.	Appl Microbiol Biotechnol	10.1007/s00253-022-11818-0	2022
	Genome-Wide Transcription Factor DNA Binding Sites and Gene Regulatory Networks in Clostridium thermocellum.	Hebdon SD, Gerritsen AT, Chen YP, Marcano JG, Chou KJ.	Front Microbiol	10.3389/fmicb.2021.695517	2021
	Utilization of Monosaccharides by Hungateiclostridium thermocellum ATCC 27405 through Adaptive Evolution.	Ha-Tran DM, Nguyen TTM, Lo SC, Huang CC	Microorganisms	10.3390/microorganisms9071445	2021
	Computational and SAXS-based structure insights of pectin acetyl esterase (CtPae12B) of family 12 carbohydrate esterase from Clostridium thermocellum ATCC 27405.	Ahmed J, Kumar K, Sharma K, Fontes CMGA, Goyal A	J Biomol Struct Dyn	10.1080/07391102.2021.1911858	2021
Enzymology	Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system.	Ha-Tran DM, Lai RY, Nguyen TTM, Huang E, Lo SC, Huang CC	PLoS One	10.1371/journal.pone.0247135	2021
Enzymology	Computational modeling and small-angle X-ray scattering based structure analysis and identifying ligand cleavage mechanism by processive endocellulase of family 9 glycoside hydrolase (HtGH9) from Hungateiclostridium thermocellum ATCC 27405.	Kumar K, Singh S, Sharma K, Goyal A	J Mol Graph Model	10.1016/j.jmgm.2020.107808	2020
Enzymology	Role of carbohydrate binding module (CBM3c) of GH9 beta-1,4 endoglucanase (Cel9W) from Hungateiclostridium thermocellum ATCC 27405 in catalysis.	Kumar K, Singal S, Goyal A	Carbohydr Res	10.1016/j.carres.2019.107782	2019
Metabolism	Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction-modification systems.	Riley LA, Ji L, Schmitz RJ, Westpheling J, Guss AM	J Ind Microbiol Biotechnol	10.1007/s10295-019-02218-x	2019
Enzymology	Construction of Cellulose Binding Domain Fusion FMN-Dependent NADH-Azoreductase and Glucose 1-Dehydrogenase for the Development of Flow Injection Analysis with Fusion Enzymes Immobilized on Cellulose.	Yano S, Hori Y, Kijima T, Konno H, Suyotha W, Takagi K, Wakayama M	J Appl Glycosci (1999)	10.5458/jag.jag.JAG-2018_0011	2019
Phylogeny	Comparative Biochemical Analysis of Cellulosomes Isolated from Clostridium clariflavum DSM 19732 and Clostridium thermocellum ATCC 27405 Grown on Plant Biomass.	Shinoda S, Kurosaki M, Kokuzawa T, Hirano K, Takano H, Ueda K, Haruki M, Hirano N	Appl Biochem Biotechnol	10.1007/s12010-018-2864-6	2018
	Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis.	Chang JJ, Anandharaj M, Ho CY, Tsuge K, Tsai TY, Ke HM, Lin YJ, Ha Tran MD, Li WH, Huang CC	Biotechnol Biofuels	10.1186/s13068-018-1151-7	2018
Biotechnology	Hyper-production of butyric acid from delignified rice straw by a novel consolidated bioprocess.	Chi X, Li J, Wang X, Zhang Y, Antwi P	Bioresour Technol	10.1016/j.biortech.2018.01.042	2018
Enzymology	SAXS and homology modelling based structure characterization of pectin methylesterase a family 8 carbohydrate esterase from Clostridium thermocellum ATCC 27405.	Rajulapati V, Sharma K, Dhillon A, Goyal A	Arch Biochem Biophys	10.1016/j.abb.2018.01.015	2018
Metabolism	Structural Insights into Thioether Bond Formation in the Biosynthesis of Sactipeptides.	Grove TL, Himes PM, Hwang S, Yumerefendi H, Bonanno JB, Kuhlman B, Almo SC, Bowers AA	J Am Chem Soc	10.1021/jacs.7b01283	2017
	Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure.	Dumitrache A, Akinosho H, Rodriguez M Jr, Meng X, Yoo CG, Natzke J, Engle NL, Sykes RW, Tschaplinski TJ, Muchero W, Ragauskas AJ, Davison BH, Brown SD	Biotechnol Biofuels	10.1186/s13068-016-0445-x	2016
Metabolism	Enhanced biohydrogen production from sugarcane bagasse by Clostridium thermocellum supplemented with CaCO3.	Tian QQ, Liang L, Zhu MJ	Bioresour Technol	10.1016/j.biortech.2015.08.111	2015
Metabolism	Butanol production from alkali-pretreated rice straw by co-culture of Clostridium thermocellum and Clostridium saccharoperbutylacetonicum.	Kiyoshi K, Furukawa M, Seyama T, Kadokura T, Nakazato A, Nakayama S	Bioresour Technol	10.1016/j.biortech.2015.03.061	2015
Metabolism	Optimization of influential nutrients during direct cellulose fermentation into hydrogen by Clostridium thermocellum.	Islam R, Sparling R, Cicek N, Levin DB	Int J Mol Sci	10.3390/ijms16023116	2015
Metabolism	Reassessment of the transhydrogenase/malate shunt pathway in Clostridium thermocellum ATCC 27405 through kinetic characterization of malic enzyme and malate dehydrogenase.	Taillefer M, Rydzak T, Levin DB, Oresnik IJ, Sparling R	Appl Environ Microbiol	10.1128/AEM.03360-14	2015
	Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellum strain.	Yee KL, Rodriguez M Jr, Thompson OA, Fu C, Wang ZY, Davison BH, Mielenz JR	Biotechnol Biofuels	10.1186/1754-6834-7-75	2014
Enzymology	Crystallization and preliminary X-ray crystallographic analysis of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405.	Goyal A, Ahmed S, Fontes CM, Najmudin S	Acta Crystallogr F Struct Biol Commun	10.1107/S2053230X14006402	2014
Metabolism	Role of transcription and enzyme activities in redistribution of carbon and electron flux in response to N(2) and H(2) sparging of open-batch cultures of Clostridium thermocellum ATCC 27405.	Carere CR, Rydzak T, Cicek N, Levin DB, Sparling R	Appl Microbiol Biotechnol	10.1007/s00253-013-5500-y	2014
Metabolism	Cellulose fermentation by Clostridium thermocellum and a mixed consortium in an automated repetitive batch reactor.	Reed PT, Izquierdo JA, Lynd LR	Bioresour Technol	10.1016/j.biortech.2013.12.051	2013
Transcriptome	Global transcriptome analysis of Clostridium thermocellum ATCC 27405 during growth on dilute acid pretreated Populus and switchgrass.	Wilson CM, Rodriguez M Jr, Johnson CM, Martin SL, Chu TM, Wolfinger RD, Hauser LJ, Land ML, Klingeman DM, Syed MH, Ragauskas AJ, Tschaplinski TJ, Mielenz JR, Brown SD	Biotechnol Biofuels	10.1186/1754-6834-6-179	2013
Metabolism	Thermostable recombinant beta-(1-->4)-mannanase from C. thermocellum: biochemical characterization and manno-oligosaccharides production.	Ghosh A, Luis AS, Bras JL, Fontes CM, Goyal A	J Agric Food Chem	10.1021/jf403111g	2013
Enzymology	A novel alpha-L-arabinofuranosidase of family 43 glycoside hydrolase (Ct43Araf) from Clostridium thermocellum.	Ahmed S, Luis AS, Bras JL, Ghosh A, Gautam S, Gupta MN, Fontes CM, Goyal A	PLoS One	10.1371/journal.pone.0073575	2013
Metabolism	Nitrogen and sulfur requirements for Clostridium thermocellum and Caldicellulosiruptor bescii on cellulosic substrates in minimal nutrient media.	Kridelbaugh DM, Nelson J, Engle NL, Tschaplinski TJ, Graham DE	Bioresour Technol	10.1016/j.biortech.2012.12.006	2012
Phylogeny	Isolation and characterization of two thermophilic cellulolytic strains of Clostridium thermocellum from a compost sample.	Lv W, Yu Z	J Appl Microbiol	10.1111/jam.12112	2013
Metabolism	Proteomic analysis of Clostridium thermocellum ATCC 27405 reveals the upregulation of an alternative transhydrogenase-malate pathway and nitrogen assimilation in cells grown on cellulose.	Burton E, Martin VJ	Can J Microbiol	10.1139/cjm-2012-0412	2012
Enzymology	Novel xylanase from a holstein cattle rumen metagenomic library and its application in xylooligosaccharide and ferulic Acid production from wheat straw.	Cheng F, Sheng J, Dong R, Men Y, Gan L, Shen L	J Agric Food Chem	10.1021/jf302337w	2012
Metabolism	Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms.	Mearls EB, Izquierdo JA, Lynd LR	BMC Microbiol	10.1186/1471-2180-12-180	2012
Transcriptome	Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress.	Yang S, Giannone RJ, Dice L, Yang ZK, Engle NL, Tschaplinski TJ, Hettich RL, Brown SD	BMC Genomics	10.1186/1471-2164-13-336	2012
Metabolism	Detection and characterization of a thermophilic biotin biosynthetic enzyme, 7-keto-8-aminopelargonic acid synthase, from various thermophiles.	Kubota T, Izumi Y	Biosci Biotechnol Biochem	10.1271/bbb.110807	2012
Metabolism	A defined growth medium with very low background carbon for culturing Clostridium thermocellum.	Holwerda EK, Hirst KD, Lynd LR	J Ind Microbiol Biotechnol	10.1007/s10295-012-1091-3	2012
Enzymology	Cloning of thermostable cellulase genes of Clostridium thermocellum and their secretive expression in Bacillus subtilis.	Liu JM, Xin XJ, Li CX, Xu JH, Bao J	Appl Biochem Biotechnol	10.1007/s12010-011-9456-z	2011
Pathogenicity	Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405).	Ellis LD, Holwerda EK, Hogsett D, Rogers S, Shao X, Tschaplinski T, Thorne P, Lynd LR	Bioresour Technol	10.1016/j.biortech.2011.09.128	2011
Metabolism	Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum.	Shao X, Raman B, Zhu M, Mielenz JR, Brown SD, Guss AM, Lynd LR	Appl Microbiol Biotechnol	10.1007/s00253-011-3492-z	2011
Metabolism	End-product induced metabolic shifts in Clostridium thermocellum ATCC 27405.	Rydzak T, Levin DB, Cicek N, Sparling R	Appl Microbiol Biotechnol	10.1007/s00253-011-3511-0	2011
Metabolism	Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation.	Raman B, McKeown CK, Rodriguez M Jr, Brown SD, Mielenz JR	BMC Microbiol	10.1186/1471-2180-11-134	2011
Enzymology	Isolation and characterization of a new cellulosome-producing Clostridium thermocellum strain.	Tachaapaikoon C, Kosugi A, Pason P, Waeonukul R, Ratanakhanokchai K, Kyu KL, Arai T, Murata Y, Mori Y	Biodegradation	10.1007/s10532-011-9486-9	2011
Enzymology	CAZymes Analysis Toolkit (CAT): web service for searching and analyzing carbohydrate-active enzymes in a newly sequenced organism using CAZy database.	Park BH, Karpinets TV, Syed MH, Leuze MR, Uberbacher EC	Glycobiology	10.1093/glycob/cwq106	2010
Metabolism	Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum.	Nakai H, Hachem MA, Petersen BO, Westphal Y, Mannerstedt K, Baumann MJ, Dilokpimol A, Schols HA, Duus JO, Svensson B	Biochimie	10.1016/j.biochi.2010.07.013	2010
Genetics	Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production.	Roberts SB, Gowen CM, Brooks JP, Fong SS	BMC Syst Biol	10.1186/1752-0509-4-31	2010
Metabolism	Effect of key factors on hydrogen production from cellulose in a co-culture of Clostridium thermocellum and Clostridium thermopalmarium.	Geng A, He Y, Qian C, Yan X, Zhou Z	Bioresour Technol	10.1016/j.biortech.2010.01.042	2010
Biotechnology	Characterization of the impact of acetate and lactate on ethanolic fermentation by Thermoanaerobacter ethanolicus.	He Q, Lokken PM, Chen S, Zhou J	Bioresour Technol	10.1016/j.biortech.2009.06.084	2009
Metabolism	Growth phase-dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405.	Rydzak T, Levin DB, Cicek N, Sparling R	J Biotechnol	10.1016/j.jbiotec.2009.01.022	2009
Metabolism	Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis.	Raman B, Pan C, Hurst GB, Rodriguez M Jr, McKeown CK, Lankford PK, Samatova NF, Mielenz JR	PLoS One	10.1371/journal.pone.0005271	2009
Metabolism	Influence of initial cellulose concentration on the carbon flow distribution during batch fermentation by Clostridium thermocellum ATCC 27405.	Islam R, Cicek N, Sparling R, Levin D	Appl Microbiol Biotechnol	10.1007/s00253-008-1763-0	2008
Metabolism	Continuous hydrogen production during fermentation of alpha-cellulose by the thermophillic bacterium Clostridium thermocellum.	Magnusson L, Cicek N, Sparling R, Levin D	Biotechnol Bioeng	10.1002/bit.22092	2009
Metabolism	Pyruvate catabolism and hydrogen synthesis pathway genes of Clostridium thermocellum ATCC 27405.	Carere CR, Kalia V, Sparling R, Cicek N, Levin DB	Indian J Microbiol	10.1007/s12088-008-0036-z	2008
Genetics	Construction and evaluation of a Clostridium thermocellum ATCC 27405 whole-genome oligonucleotide microarray.	Brown SD, Raman B, McKeown CK, Kale SP, He Z, Mielenz JR	Appl Biochem Biotechnol	10.1007/s12010-007-9087-6	2007
Metabolism	Mutations in the scaffoldin gene, cipA, of Clostridium thermocellum with impaired cellulosome formation and cellulose hydrolysis: insertions of a new transposable element, IS1447, and implications for cellulase synergism on crystalline cellulose.	Zverlov VV, Klupp M, Krauss J, Schwarz WH	J Bacteriol	10.1128/JB.00097-08	2008
Enzymology	Cloning and characterization of a heat-stable CMP-N-acylneuraminic acid synthetase from Clostridium thermocellum.	Mizanur RM, Pohl NL	Appl Microbiol Biotechnol	10.1007/s00253-007-1053-2	2007
Metabolism	The functional repertoire of prokaryote cellulosomes includes the serpin superfamily of serine proteinase inhibitors.	Kang S, Barak Y, Lamed R, Bayer EA, Morrison M	Mol Microbiol	10.1111/j.1365-2958.2006.05182.x	2006
Metabolism	Expression of 17 genes in Clostridium thermocellum ATCC 27405 during fermentation of cellulose or cellobiose in continuous culture.	Stevenson DM, Weimer PJ	Appl Environ Microbiol	10.1128/AEM.71.8.4672-4678.2005	2005
Metabolism	Wood adhesives prepared from lucerne fiber fermentation residues of Ruminococcus albus and Clostridium thermocellum.	Weimer PJ, Koegel RG, Lorenz LF, Frihart CR, Kenealy WR	Appl Microbiol Biotechnol	10.1007/s00253-004-1767-3	2004
Metabolism	Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum.	Zhang YH, Lynd LR	Appl Environ Microbiol	10.1128/AEM.70.3.1563-1569.2004	2004
Metabolism	Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-beta-1,3-glucanase bound to the outer cell surface.	Fuchs KP, Zverlov VV, Velikodvorskaya GA, Lottspeich F, Schwarz WH	Microbiology (Reading)	10.1099/mic.0.26153-0	2003
Enzymology	Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an enzyme-linked immunosorbent assay-based method with application to Clostridium thermocellum batch cultures.	Zhang Y, Lynd LR	Anal Chem	10.1021/ac020271n	2003
Enzymology	Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria.	Ozkan M, Desai SG, Zhang Y, Stevenson DM, Beane J, White EA, Guerinot ML, Lynd LR	J Ind Microbiol Biotechnol	10.1038/sj.jim.7000082	2001
Enzymology	Restriction endonuclease activity in Clostridium thermocellum and Clostridium thermosaccharolyticum.	Klapatch TR, Demain AL, Lynd LR	Appl Microbiol Biotechnol	10.1007/s002530050659	1996
Enzymology	Cloning and expression of the Clostridium thermocellum celS gene in Escherichia coli.	Wang WK, Kruus K, Wu JH	Appl Microbiol Biotechnol	10.1007/BF00902740	1994
Enzymology	Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase Ss (CelS), a major cellulosome component.	Wang WK, Kruus K, Wu JH	J Bacteriol	10.1128/jb.175.5.1293-1302.1993	1993
Metabolism	Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS.	Poole DM, Morag E, Lamed R, Bayer EA, Hazlewood GP, Gilbert HJ	FEMS Microbiol Lett	10.1016/0378-1097(92)90022-g	1992
Biotechnology	Effect of Yeast Extract and Vitamin B(12) on Ethanol Production from Cellulose by Clostridium thermocellum I-1-B.	Sato K, Goto S, Yonemura S, Sekine K, Okuma E, Takagi Y, Hon-Nami K, Saiki T	Appl Environ Microbiol	10.1128/aem.58.2.734-736.1992	1992
	Primary structure of O-linked carbohydrate chains in the cellulosome of different Clostridium thermocellum strains.	Gerwig GJ, Kamerling JP, Vliegenthart JF, Morag E, Lamed R, Bayer EA	Eur J Biochem	10.1111/j.1432-1033.1991.tb15793.x	1991
	Bioconversion of cellulose into ethanol by Clostridium thermocellum--product inhibition.	Kundu S, Ghose TK, Mukhopadhyay SN	Biotechnol Bioeng	10.1002/bit.260250418	1983
Metabolism	Ethanol-induced changes in the membrane lipid composition of Clostridium thermocellum.	Herrero AA, Gomez RF, Roberts MF	Biochim Biophys Acta	10.1016/0005-2736(82)90487-4	1982
Pathogenicity	Development of ethanol tolerance in Clostridium thermocellum: effect of growth temperature.	Herrero AA, Gomez RF	Appl Environ Microbiol	10.1128/aem.40.3.571-577.1980	1980
Genetics	Draft genome sequence data of Clostridium thermocellum PAL5 possessing high cellulose-degradation ability.	Nakazono-Nagaoka E, Fujikawa T, Shikata A, Tachaapaikoon C, Waeonukul R, Pason P, Ratanakhanokchai K, Kosugi A	Data Brief	10.1016/j.dib.2019.104274	2019
Metabolism	LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313.	Wilson CM, Klingeman DM, Schlachter C, Syed MH, Wu CW, Guss AM, Brown SD	Appl Environ Microbiol	10.1128/AEM.02751-16	2017
Genetics	Complete genome sequence of the cellulolytic thermophile Clostridium thermocellum DSM1313.	Feinberg L, Foden J, Barrett T, Davenport KW, Bruce D, Detter C, Tapia R, Han C, Lapidus A, Lucas S, Cheng JF, Pitluck S, Woyke T, Ivanova N, Mikhailova N, Land M, Hauser L, Argyros DA, Goodwin L, Hogsett D, Caiazza N	J Bacteriol	10.1128/JB.00322-11	2011
Stress	Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes.	Tyurin MV, Sullivan CR, Lynd LR	Appl Environ Microbiol	10.1128/AEM.71.12.8069-8076.2005	2005
Pathogenicity	Electrotransformation of Clostridium thermocellum.	Tyurin MV, Desai SG, Lynd LR	Appl Environ Microbiol	10.1128/AEM.70.2.883-890.2004	2004
Phylogeny	Clostridium clariflavum sp. nov. and Clostridium caenicola sp. nov., moderately thermophilic, cellulose-/cellobiose-digesting bacteria isolated from methanogenic sludge.	Shiratori H, Sasaya K, Ohiwa H, Ikeno H, Ayame S, Kataoka N, Miya A, Beppu T, Ueda K	Int J Syst Evol Microbiol	10.1099/ijs.0.003483-0	2009

References

#545	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1237
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#22909	Shuangya Chen, Lili Niu, Yongxiang Zhang: Saccharofermentans acetigenes gen. nov., sp. nov., an anaerobic bacterium isolated from sludge treating brewery wastewater. IJSEM 60: 2735 - 2738 2010 ( DOI 10.1099/ijs.0.017590-0 , PubMed 20061495 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acetivibrio thermocellus (DSM 1237, ATCC 27405, JCM 9322)

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