Acetivibrio thermocellus (DSM 4150, ATCC 31549, JW 20)

Name: Acetivibrio thermocellus (DSM 4150, ATCC 31549, JW 20)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2731

Type strain

Strain Designation JW 20

Culture col. no. DSM 4150 ATCC 31549 JW 20

NCBI tax ID(s) 1515

Special Collections DSMZ

History <- A. Nolte, Universität Göttingen, Institut für Mikrobiologie, Germany; JW 20 <- J. Wiegel

Links

Acetivibrio thermocellus JW 20 is an anaerobe, thermophilic prokaryote that produces alcohol and was isolated from cotton.

alcohol production anaerobe thermophilic genome sequence

Name and taxonomic classification

SI-ID 40118

ATCC 31549,KCTC 1673

@ref 20215

Last LPSN update 2025-12-16 09:47:22

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Oscillospiraceae

Genus Acetivibrio

Species Acetivibrio thermocellus

Full scientific name Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019

Synonyms (3)

Clostridium thermocellum
"Ruminiclostridium thermocellum"
Hungateiclostridium thermocellum

BacDive ID	Other strains from **Acetivibrio thermocellus** (6)	Type strain
2729	A. thermocellus DSM 1237, ATCC 27405, JCM 9322, NCIMB 10682, ... (type strain)
2728	A. thermocellus LQ8, DSM 1313
2730	A. thermocellus LQR1, DSM 2360, JCM 9323, ATCC 35609
22965	A. thermocellus YS, DSM 25991
22966	A. thermocellus AD2, DSM 25992
174402	A. thermocellus DSM 29190, BC 1

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1603	ACETIVIBRIO MEDIUM (DSMZ Medium 122)		Medium recipe at MediaDive	Name: ACETIVIBRIO MEDIUM (DSMZ Medium 122) Composition: Cellulose 10.0 g/l (optional) Na2-ß-glycerophosphate x 5 H2O 6.0 g/l K2HPO4 5.5 g/l Cellobiose 5.0 g/l Yeast extract 4.5 g/l MgCl2 x 6 H2O 2.6 g/l KH2PO4 1.43 g/l (NH4)2SO4 1.3 g/l L-Glutathione 0.25 g/l CaCl2 x 2 H2O 0.13 g/l FeSO4 x 7 H2O 0.0011 g/l Sodium resazurin 0.0005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1603	positive	growth	60	thermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
1603	anaerobe
125438	anaerobe	92.235
125439	anaerobe	99.3

Compound production

1603

Compoundethanol

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Shrub (Scrub)
#Engineered	#Industrial	#Engineered product
#Host Body Product	#Plant	#Cotton (other fibres)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1603	cotton	Louisiana	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1603	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID
66792	ASM17301v1 assembly for Acetivibrio thermocellus JW20	contig	GCA_000173015	492476.4	642791610	492476

GC content

1603

GC-content (mol%)40.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Clostridium thermocellum JW20
PATRIC: 492476.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	69.50	no
125439	motility	BacteriaNetⓘ	yes	69.40	no
125439	gram_stain	BacteriaNetⓘ	variable	85.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Clostridium thermocellum JW20
PATRIC: 492476.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	54.63	no
125438	anaerobic	anaerobicⓘ	yes	92.24	no
125438	spore-forming	spore-formingⓘ	yes	76.07	no
125438	aerobic	aerobicⓘ	no	98.12	yes
125438	thermophilic	thermophileⓘ	yes	57.88	no
125438	flagellated	motile2+ⓘ	yes	68.97	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose-Degrading Anaerobic Bacteria.	Ishida K, Krabbe J, Meisinger PR, Shabuer G, Schieferdecker S, Cyrulies M, Tank C, Barnes E, Paetz C, Hertweck C.	Angew Chem Int Ed Engl	10.1002/anie.202503697	2025
Phylogeny	Clostridium thermocellum JW20 (ATCC 31549) is a coculture with Thermoanaerobacter ethanolicus.	Erbeznik M, Jones CR, Dawson KA, Strobel HJ	Appl Environ Microbiol	10.1128/aem.63.7.2949-2951.1997	1997
	Characterization of Clostridium thermocellum JW20.	Freier D, Mothershed CP, Wiegel J	Appl Environ Microbiol	10.1128/aem.54.1.204-211.1988	1988
Enzymology	Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria.	Ozkan M, Desai SG, Zhang Y, Stevenson DM, Beane J, White EA, Guerinot ML, Lynd LR	J Ind Microbiol Biotechnol	10.1038/sj.jim.7000082	2001

References

#1603	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4150
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Acetivibrio thermocellus (DSM 4150, ATCC 31549, JW 20)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000173015

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media