Strain identifier

BacDive ID: 2731

Type strain: No

Species: Acetivibrio thermocellus

Strain Designation: JW 20

Strain history: <- A. Nolte, Universität Göttingen, Institut für Mikrobiologie, Germany; JW 20 <- J. Wiegel

NCBI tax ID(s): 1515 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1603

BacDive-ID: 2731

DSM-Number: 4150

keywords: genome sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive, alcohol production

description: Acetivibrio thermocellus JW 20 is an anaerobe, spore-forming, thermophilic bacterium that produces alcohol and was isolated from cotton.

NCBI tax id

  • NCBI tax id: 1515
  • Matching level: species

strain history

  • @ref: 1603
  • history: <- A. Nolte, Universität Göttingen, Institut für Mikrobiologie, Germany; JW 20 <- J. Wiegel

doi: 10.13145/bacdive2731.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Oscillospiraceae
  • genus: Acetivibrio
  • species: Acetivibrio thermocellus
  • full scientific name: Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019
  • synonyms

    @refsynonym
    20215Ruminiclostridium thermocellum
    20215Clostridium thermocellum
    20215Hungateiclostridium thermocellum

@ref: 1603

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Oscillospiraceae

genus: Acetivibrio

species: Acetivibrio thermocellus

full scientific name: Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019

strain designation: JW 20

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 99.745

Culture and growth conditions

culture medium

  • @ref: 1603
  • name: ACETIVIBRIO MEDIUM (DSMZ Medium 122)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/122
  • composition: Name: ACETIVIBRIO MEDIUM (DSMZ Medium 122) Composition: Cellulose 10.0 g/l (optional) Na2-ß-glycerophosphate x 5 H2O 6.0 g/l K2HPO4 5.5 g/l Cellobiose 5.0 g/l Yeast extract 4.5 g/l MgCl2 x 6 H2O 2.6 g/l KH2PO4 1.43 g/l (NH4)2SO4 1.3 g/l L-Glutathione 0.25 g/l CaCl2 x 2 H2O 0.13 g/l FeSO4 x 7 H2O 0.0011 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
1603positivegrowth60thermophilic
69480thermophilic96.668

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
1603anaerobe
69480anaerobe99.999

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 98.769

compound production

  • @ref: 1603
  • compound: ethanol

Isolation, sampling and environmental information

isolation

  • @ref: 1603
  • sample type: cotton
  • geographic location: Louisiana
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Shrub (Scrub)
#Engineered#Industrial#Engineered product
#Host Body Product#Plant#Cotton (other fibres)

Safety information

risk assessment

  • @ref: 1603
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Clostridium thermocellum JW20
  • accession: 492476.4
  • assembly level: wgs
  • database: patric
  • NCBI tax ID: 492476

GC content

  • @ref: 1603
  • GC-content: 40.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes76no
gram-positiveno52.849no
anaerobicyes99.568no
halophileno95.085no
spore-formingyes90.095no
glucose-utilyes83.143no
thermophileyes99.058no
motileno55.798no
aerobicno98.703yes
flagellatedno77.728no
glucose-fermentno51.578no

External links

@ref: 1603

culture collection no.: DSM 4150, ATCC 31549

straininfo link

@refstraininfo
7225940118
7226047799

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9212442Clostridium thermocellum JW20 (ATCC 31549) is a coculture with Thermoanaerobacter ethanolicus.Erbeznik M, Jones CR, Dawson KA, Strobel HJAppl Environ Microbiol10.1128/aem.63.7.2949-2951.19971997Bacteria, Anaerobic/*genetics/*isolation & purification, Clostridium/*genetics/*isolation & purification, Coculture Techniques, Gram-Positive Asporogenous Rods, Irregular/*genetics/*isolation & purification, Molecular Sequence Data, Oligonucleotide Probes/genetics, Polymerase Chain Reaction/*methods, RNA, Ribosomal, 16S/*genetics, Sensitivity and Specificity, Soil MicrobiologyEnzymology
Enzymology11781801Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria.Ozkan M, Desai SG, Zhang Y, Stevenson DM, Beane J, White EA, Guerinot ML, Lynd LRJ Ind Microbiol Biotechnol10.1038/sj.jim.70000822001Bacteria, Anaerobic/genetics/*isolation & purification/metabolism, Cellulose/*metabolism, Hot Temperature, Hydrogenase/genetics, Microbial Sensitivity Tests, Molecular Sequence Data, Phosphate Acetyltransferase/genetics, Polymerase Chain Reaction, RNA, Ribosomal, 16S/geneticsPhylogeny
16347527Characterization of Clostridium thermocellum JW20.Freier D, Mothershed CP, Wiegel JAppl Environ Microbiol10.1128/aem.54.1.204-211.19881988

Reference

@idauthorscataloguedoi/urltitle
1603Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4150)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4150
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
72259Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40118.1StrainInfo: A central database for resolving microbial strain identifiers
72260Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47799.1StrainInfo: A central database for resolving microbial strain identifiers