Acetivibrio thermocellus (DSM 1313, LQ8)

Name: Acetivibrio thermocellus (DSM 1313, LQ8)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2728

Type strain

Strain Designation LQ8

Culture col. no. DSM 1313 LQ8

NCBI tax ID(s) 637887 1515

Special Collections DSMZ Literature strains

History DSMZ <- J. G. Zeikus, Universityof Wisconsin, Departments of Biochemistry and Agricultural Bacteriology, Madison, USA; LQ8 <- L. Y. Quinn;

Links

Acetivibrio thermocellus LQ8 is an anaerobe bacterium that produces alcohol.

alcohol production anaerobe genome sequence Bacteria

Name and taxonomic classification

SI-ID 1975

LMG 6656,DSM 1313

@ref 20215

Last LPSN update 2026-05-29 08:25:32

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Oscillospiraceae

Genus Acetivibrio

Species Acetivibrio thermocellus

Full scientific name Acetivibrio thermocellus (Viljoen et al. 1926) Tindall 2019

Synonyms (3)

Clostridium thermocellum
"Ruminiclostridium thermocellum"
Hungateiclostridium thermocellum

BacDive ID	Other strains from **Acetivibrio thermocellus** (6)	Type strain
2729	A. thermocellus DSM 1237, ATCC 27405, JCM 9322, NCIMB 10682, ... (type strain)
2730	A. thermocellus LQR1, DSM 2360, JCM 9323, ATCC 35609
2731	A. thermocellus JW 20, DSM 4150, ATCC 31549
22965	A. thermocellus YS, DSM 25991
22966	A. thermocellus AD2, DSM 25992
174402	A. thermocellus DSM 29190, BC 1

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
511	ACETIVIBRIO MEDIUM (DSMZ Medium 122)		Medium recipe at MediaDive	Name: ACETIVIBRIO MEDIUM (DSMZ Medium 122) Composition: Cellulose 10.0 g/l (optional) Na2-ß-glycerophosphate x 5 H2O 6.0 g/l K2HPO4 5.5 g/l Cellobiose 5.0 g/l Yeast extract 4.5 g/l MgCl2 x 6 H2O 2.6 g/l KH2PO4 1.43 g/l (NH4)2SO4 1.3 g/l L-Glutathione 0.25 g/l CaCl2 x 2 H2O 0.13 g/l FeSO4 x 7 H2O 0.0011 g/l Sodium resazurin 0.0005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
511	positive	growth	60

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
511	anaerobe
125438	anaerobe	95.236
125439	facultative anaerobe	97.346

Compound production

511

Compoundethanol

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43410	17057 ChEBI	cellobiose	+	degradation
43410	62968 ChEBI	cellulose	+	degradation
43410	17234 ChEBI	glucose	+	growth

Isolation, sampling and environmental information

Isolation

@ref	Geographic location	Country	Country ISO 3 Code	Continent
511	Iowa	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
511	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Acetivibrio thermocellus DSM 1313	complete			2825454334	1515	99.38
66792	ASM18492v1 assembly for Acetivibrio thermocellus DSM 1313	complete	GCA_000184925	637887.3	650377924	637887	98.06
66792	Hungateiclostridium thermocellum strain DSM 1313	contig		1515.11		1515	79.33

GC content

511

GC-content (mol%)39.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM18492v1 assembly for Acetivibrio thermocellus DSM 1313
BV-BRC: 637887.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	97.35	no
125439	gram_stain	BacteriaNetⓘ	variable	61.78	no
125439	motility	BacteriaNetⓘ	yes	76.44	no
125439	spore_formation	BacteriaNetⓘ	yes	79.72	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acetivibrio thermocellus DSM 1313 DSM 1313
NCBI: GCA_000184925.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	57.41	no
125438	anaerobic	anaerobicⓘ	yes	95.24	no
125438	aerobic	aerobicⓘ	no	97.94	yes
125438	spore-forming	spore-formingⓘ	yes	83.94	no
125438	thermophilic	thermophileⓘ	yes	68.06	no
125438	flagellated	motile2+ⓘ	yes	77.71	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose-Degrading Anaerobic Bacteria.	Ishida K, Krabbe J, Meisinger PR, Shabuer G, Schieferdecker S, Cyrulies M, Tank C, Barnes E, Paetz C, Hertweck C.	Angew Chem Int Ed Engl	10.1002/anie.202503697	2025
Transcriptome	Metabolic division of labor between Acetivibrio thermocellus DSM 1313 and Thermoanaerobacterium thermosaccharolyticum MJ1 enhanced hydrogen production from lignocellulose.	Mai J, Hu BB, Zhu MJ.	Bioresour Technol	10.1016/j.biortech.2023.129871	2023
	Enhanced bioelectricity generation in thermophilic microbial fuel cell with lignocellulose as an electron donor by resazurin-mediated electron transfer.	Yan X, Zhu MJ.	Bioresour Technol	10.1016/j.biortech.2023.129764	2023
	Fermentative conversion of unpretreated plant biomass: A thermophilic threshold for indigenous microbial growth.	Bing RG, Carey MJ, Laemthong T, Willard DJ, Crosby JR, Sulis DB, Wang JP, Adams MWW, Kelly RM.	Bioresour Technol	10.1016/j.biortech.2022.128275	2023
	Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers.	Sharma BD, Hon S, Thusoo E, Stevenson DM, Amador-Noguez D, Guss AM, Lynd LR, Olson DG.	Biotechnol Biofuels Bioprod	10.1186/s13068-024-02591-5	2024
	The Roles of Nicotinamide Adenine Dinucleotide Phosphate Reoxidation and Ammonium Assimilation in the Secretion of Amino Acids as Byproducts of Clostridium thermocellum.	Yayo J, Rydzak T, Kuil T, Karlsson A, Harding DJ, Guss AM, van Maris AJA.	Appl Environ Microbiol	10.1128/aem.01753-22	2023
Metabolism	Laboratory Evolution and Reverse Engineering of Clostridium thermocellum for Growth on Glucose and Fructose.	Yayo J, Kuil T, Olson DG, Lynd LR, Holwerda EK, van Maris AJA.	Appl Environ Microbiol	10.1128/aem.03017-20	2021
	Antibacterial Synthetic Nanocelluloses Synergizing with a Metal-Chelating Agent.	Serizawa T, Yamaguchi S, Sugiura K, Marten R, Yamamoto A, Hata Y, Sawada T, Tanaka H, Tanaka M.	ACS Appl Bio Mater	10.1021/acsabm.3c00846	2024
	Surface-mediated self-assembly of click-reactive cello-oligosaccharides for fabricating functional nonwoven fabrics.	Mizuuchi Y, Hata Y, Sawada T, Serizawa T.	Sci Technol Adv Mater	10.1080/14686996.2024.2311052	2024
Enzymology	Ethanol tolerance in engineered strains of Clostridium thermocellum.	Olson DG, Maloney MI, Lanahan AA, Cervenka ND, Xia Y, Pech-Canul A, Hon S, Tian L, Ziegler SJ, Bomble YJ, Lynd LR.	Biotechnol Biofuels Bioprod	10.1186/s13068-023-02379-z	2023
	Genome-Wide Transcription Factor DNA Binding Sites and Gene Regulatory Networks in Clostridium thermocellum.	Hebdon SD, Gerritsen AT, Chen YP, Marcano JG, Chou KJ.	Front Microbiol	10.3389/fmicb.2021.695517	2021
	Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms.	Rodriguez-Torres LM, Huerta-Miranda GA, Martinez-Garcia AL, Mazon-Montijo DA, Hernandez-Eligio A, Miranda-Hernandez M, Juarez K.	Environ Sci Pollut Res Int	10.1007/s11356-024-33612-3	2025
Genetics	A genomic analysis reveals the diversity of cellulosome displaying bacteria.	Minor CM, Takayesu A, Ha SM, Salwinski L, Sawaya MR, Pellegrini M, Clubb RT.	Front Microbiol	10.3389/fmicb.2024.1473396	2024
	Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation.	Ichikawa S, Tsuge Y, Karita S.	Microorganisms	10.3390/microorganisms9030593	2021
Genetics	Utilization of Monosaccharides by Hungateiclostridium thermocellum ATCC 27405 through Adaptive Evolution.	Ha-Tran DM, Nguyen TTM, Lo SC, Huang CC.	Microorganisms	10.3390/microorganisms9071445	2021
	The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.	Mahoney BJ, Takayesu A, Zhou A, Cascio D, Clubb RT.	Proteins	10.1002/prot.26326	2022
Enzymology	Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles.	Daley SR, Gallanosa PM, Sparling R.	Biotechnol Biofuels Bioprod	10.1186/s13068-023-02362-8	2023
	Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum.	Yan F, Dong S, Liu YJ, Yao X, Chen C, Xiao Y, Bayer EA, Shoham Y, You C, Cui Q, Feng Y.	mBio	10.1128/mbio.01476-22	2022
	Characterization of Two alpha-l-Arabinofuranosidases from Acetivibrio mesophilus and Their Synergistic Effect in Degradation of Arabinose-Containing Substrates.	Liu Y, Vanderhaeghen S, Feiler W, Angelov A, Baudrexl M, Zverlov V, Liebl W.	Microorganisms	10.3390/microorganisms9071467	2021
Biotechnology	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.	Biswal AK, Hengge NN, Black IM, Atmodjo MA, Mohanty SS, Ryno D, Himmel ME, Azadi P, Bomble YJ, Mohnen D	Biotechnol Biofuels Bioprod	10.1186/s13068-022-02119-9	2022
Biotechnology	Identification and characterization of proteins of unknown function (PUFs) in Clostridium thermocellum DSM 1313 strains as potential genetic engineering targets.	Poudel S, Cope AL, O'Dell KB, Guss AM, Seo H, Trinh CT, Hettich RL	Biotechnol Biofuels	10.1186/s13068-021-01964-4	2021
	NMR chemical shift assignments of a module of unknown function in the cellulosomal secondary scaffoldin ScaF from Clostridium thermocellum.	Li J, Chen C, Liu YJ, Cui Q, Bayer EA, Feng Y	Biomol NMR Assign	10.1007/s12104-021-10025-8	2021
Metabolism	Construction of lactic acid overproducing Clostridium thermocellum through enhancement of lactate dehydrogenase expression.	Mazzoli R, Olson DG, Lynd LR	Enzyme Microb Technol	10.1016/j.enzmictec.2020.109645	2020
	CRISPR interference (CRISPRi) as transcriptional repression tool for Hungateiclostridium thermocellum DSM 1313.	Ganguly J, Martin-Pascual M, van Kranenburg R	Microb Biotechnol	10.1111/1751-7915.13516	2019
Genetics	Draft genome sequence data of Clostridium thermocellum PAL5 possessing high cellulose-degradation ability.	Nakazono-Nagaoka E, Fujikawa T, Shikata A, Tachaapaikoon C, Waeonukul R, Pason P, Ratanakhanokchai K, Kosugi A	Data Brief	10.1016/j.dib.2019.104274	2019
	Resonance assignments of a cellulosomal double-dockerin from Clostridium thermocellum.	Chen C, Yang H, Xuan J, Cui Q, Feng Y	Biomol NMR Assign	10.1007/s12104-018-9859-7	2018
	Clostridium thermocellum LL1210 pH homeostasis mechanisms informed by transcriptomics and metabolomics.	Whitham JM, Moon JW, Rodriguez M Jr, Engle NL, Klingeman DM, Rydzak T, Abel MM, Tschaplinski TJ, Guss AM, Brown SD	Biotechnol Biofuels	10.1186/s13068-018-1095-y	2018
Metabolism	Engineering cellulolytic bacterium Clostridium thermocellum to co-ferment cellulose- and hemicellulose-derived sugars simultaneously.	Xiong W, Reyes LH, Michener WE, Maness PC, Chou KJ	Biotechnol Bioeng	10.1002/bit.26590	2018
	Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum.	Liu YJ, Liu S, Dong S, Li R, Feng Y, Cui Q	Biotechnol Biofuels	10.1186/s13068-017-1009-4	2018
	How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?	Yoav S, Barak Y, Shamshoum M, Borovok I, Lamed R, Dassa B, Hadar Y, Morag E, Bayer EA	Biotechnol Biofuels	10.1186/s13068-017-0909-7	2017
Metabolism	Overflow metabolism and growth cessation in Clostridium thermocellum DSM1313 during high cellulose loading fermentations.	Thompson RA, Trinh CT	Biotechnol Bioeng	10.1002/bit.26374	2017
	Enhanced saccharification of lignocellulosic agricultural biomass and increased bioethanol titre using acclimated Clostridium thermocellum DSM1313.	Nisha M, Saranyah K, Shankar M, Saleena LM	3 Biotech	10.1007/s13205-017-0606-z	2017
Biotechnology	Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring.	Singh N, Mathur AS, Tuli DK, Gupta RP, Barrow CJ, Puri M	Biotechnol Biofuels	10.1186/s13068-017-0756-6	2017
Metabolism	LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313.	Wilson CM, Klingeman DM, Schlachter C, Syed MH, Wu CW, Guss AM, Brown SD	Appl Environ Microbiol	10.1128/AEM.02751-16	2017
Phenotype	Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome.	Thompson RA, Dahal S, Garcia S, Nookaew I, Trinh CT	Biotechnol Biofuels	10.1186/s13068-016-0607-x	2016
Metabolism	Genetic engineering of Clostridium thermocellum DSM1313 for enhanced ethanol production.	Kannuchamy S, Mukund N, Saleena LM	BMC Biotechnol	10.1186/s12896-016-0260-2	2016
	Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation.	Sander K, Wilson CM, Rodriguez M Jr, Klingeman DM, Rydzak T, Davison BH, Brown SD	Biotechnol Biofuels	10.1186/s13068-015-0394-9	2015
Enzymology	Expression and characterization of a new thermostable esterase from Clostridium thermocellum.	Zhang T, Chen H, Ni Z, Tian R, Jia J, Chen Z, Yang S	Appl Biochem Biotechnol	10.1007/s12010-015-1824-7	2015
Metabolism	Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways.	van der Veen D, Lo J, Brown SD, Johnson CM, Tschaplinski TJ, Martin M, Engle NL, van den Berg RA, Argyros AD, Caiazza NC, Guss AM, Lynd LR	J Ind Microbiol Biotechnol	10.1007/s10295-013-1275-5	2013
	Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum.	Guss AM, Olson DG, Caiazza NC, Lynd LR	Biotechnol Biofuels	10.1186/1754-6834-5-30	2012
Genetics	Complete genome sequence of the cellulolytic thermophile Clostridium thermocellum DSM1313.	Feinberg L, Foden J, Barrett T, Davenport KW, Bruce D, Detter C, Tapia R, Han C, Lapidus A, Lucas S, Cheng JF, Pitluck S, Woyke T, Ivanova N, Mikhailova N, Land M, Hauser L, Argyros DA, Goodwin L, Hogsett D, Caiazza N	J Bacteriol	10.1128/JB.00322-11	2011
Stress	Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes.	Tyurin MV, Sullivan CR, Lynd LR	Appl Environ Microbiol	10.1128/AEM.71.12.8069-8076.2005	2005
Pathogenicity	Electrotransformation of Clostridium thermocellum.	Tyurin MV, Desai SG, Lynd LR	Appl Environ Microbiol	10.1128/AEM.70.2.883-890.2004	2004

References

#511	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1313
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43410	Shahar Yoav, Yoav Barak, Melina Shamshoum, Ilya Borovok, Raphael Lamed, Bareket Dassa, Yitzhak Hadar, Ely Morag and Edward A. Baye: How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?. Biotechnol Biofuels 10: 2017 ( DOI 10.1186/s13068-017-0909-7 , PubMed 28932263 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acetivibrio thermocellus (DSM 1313, LQ8)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Metabolite utilization

Isolation, sampling and environmental information

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000184925

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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