Acetivibrio cellulolyticus (DSM 1870, ATCC 33288, NRC 2248)

Name: Acetivibrio cellulolyticus (DSM 1870, ATCC 33288, NRC 2248)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17674

Type strain

Strain Designation CD2

Culture col. no. DSM 1870 ATCC 33288 NRC 2248 CD2 HKI 0653 2 more

NCBI tax ID(s) 509191 35830

Special Collections DSMZ Literature strains JMRC

History DSMZ <- G. B. Patel, National Research Council, Ottawa, Canada; CD2 <- G. B. Patel; {1978}

Links

Acetivibrio cellulolyticus CD2 is an anaerobe bacterium that was isolated from sewage sludge.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 10241

LMG 8466,ATCC 33288,DSM 1870

@ref 20215

Last LPSN update 2026-05-29 08:29:04

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Oscillospiraceae

Genus Acetivibrio

Species Acetivibrio cellulolyticus

Full scientific name Acetivibrio cellulolyticus Patel et al. 1980

Synonyms (2)

Acetivibrio cellulosolvens
Hungateiclostridium cellulolyticum

Morphology

Colony morphology

916

Incubation period8-14 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
916	ACETIVIBRIO CELLULOLYTICUS MEDIUM (DSMZ Medium 165)		Medium recipe at MediaDive	Name: ACETIVIBRIO CELLULOLYTICUS MEDIUM (DSMZ Medium 165) Composition: Cellulose 2.997 g/l (optional) Cellobiose 2.997 g/l Na2CO3 1.4985 g/l (NH4)2SO4 0.44955 g/l K2HPO4 0.292208 g/l L-Cysteine HCl x H2O 0.24975 g/l Na2S x 9 H2O 0.24975 g/l KH2PO4 0.17982 g/l MgSO4 x 7 H2O 0.02997 g/l Nitrilotriacetic acid 0.014985 g/l NaCl 0.00999001 g/l MnSO4 x H2O 0.00499501 g/l CoSO4 x 7 H2O 0.0017982 g/l ZnSO4 x 7 H2O 0.0017982 g/l FeSO4 x 7 H2O 0.000999001 g/l CaCl2 x 2 H2O 0.000999001 g/l Sodium resazurin 0.0004995 g/l NiCl2 x 6 H2O 0.0002997 g/l AlK(SO4)2 x 12 H2O 0.0001998 g/l Na2MoO4 x 2 H2O 9.99001e-05 g/l H3BO3 9.99001e-05 g/l CuSO4 x 5 H2O 9.99001e-05 g/l Pyridoxine hydrochloride 9.99001e-05 g/l p-Aminobenzoic acid 4.995e-05 g/l (DL)-alpha-Lipoic acid 4.995e-05 g/l Thiamine HCl 4.995e-05 g/l Riboflavin 4.995e-05 g/l Nicotinic acid 4.995e-05 g/l Calcium D-(+)-pantothenate 4.995e-05 g/l Biotin 1.998e-05 g/l Folic acid 1.998e-05 g/l Na2WO4 x 2 H2O 3.996e-06 g/l Na2SeO3 x 5 H2O 2.997e-06 g/l Vitamin B12 9.99001e-07 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
916	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
916	anaerobe
125438	anaerobe	93.049
125439	facultative anaerobe	95.841

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68367	17057 ChEBI	cellobiose	+	builds acid from	from API 20A
43408	62968 ChEBI	cellulose	+	degradation
43408	62968 ChEBI	cellulose	+	hydrolysis
68367	17634 ChEBI	D-glucose	-	builds acid from	from API 20A
68367	16899 ChEBI	D-mannitol	-	builds acid from	from API 20A
68367	16024 ChEBI	D-mannose	-	builds acid from	from API 20A
68367	65327 ChEBI	D-xylose	-	builds acid from	from API 20A
68367	4853 ChEBI	esculin	+	hydrolysis	from API 20A
68367	5291 ChEBI	gelatin	+	hydrolysis	from API 20A
68367	17754 ChEBI	glycerol	-	builds acid from	from API 20A
68367	30849 ChEBI	L-arabinose	-	builds acid from	from API 20A
68367	62345 ChEBI	L-rhamnose	-	builds acid from	from API 20A
68367	17716 ChEBI	lactose	-	builds acid from	from API 20A
68367	17306 ChEBI	maltose	-	builds acid from	from API 20A
68367	6731 ChEBI	melezitose	-	builds acid from	from API 20A
68367	16634 ChEBI	raffinose	-	builds acid from	from API 20A
68367	17814 ChEBI	salicin	-	builds acid from	from API 20A
68367	30911 ChEBI	sorbitol	-	builds acid from	from API 20A
68367	17992 ChEBI	sucrose	-	builds acid from	from API 20A
68367	27082 ChEBI	trehalose	-	builds acid from	from API 20A
68367	27897 ChEBI	tryptophan	-	energy source	from API 20A
68367	16199 ChEBI	urea	-	hydrolysis	from API 20A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68367	35581 ChEBI	indole		from API 20A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68367	35581 ChEBI	indole	-	from API 20A

Enzymes

@ref	Value	Activity	Ec
68367	beta-glucosidase	+	3.2.1.21	from API 20A
68367	catalase	-	1.11.1.6	from API 20A
43408	cellulase	+	3.2.1.4
68367	gelatinase	+		from API 20A
68367	urease	-	3.5.1.5	from API 20A

API 20A

@ref	IND	URE	GLU	MAN	LAC	SAC	MAL	SAL	XYL	ARA	GEL	ESC	GLY	CEL	MNE	MLZ	RAF	SOR	RHA	TRE	CAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
916	-	-	-	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Sewage sludge

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
916	sewage sludge	Ontario, Ottawa	Canada	CAN	North America

Taxonmaps

69479

Global distribution of 16S sequence NR_025917 (>99% sequence identity) for Acetivibrio cellulolyticus from Microbeatlas

Aquatic Samples	545
Soil Samples	600
Animal Samples	10240
Plant Samples	146
Total Samples	11531

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
916	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM17959v2 assembly for Acetivibrio cellulolyticus CD2	scaffold	GCA_000179595	509191.4	2510461053	509191	53.85

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Acetivibrio cellulolyticus (ATCC 33288) 16S ribosomal RNA (16S rRNA)	L35516	1464		35830
916	Acetivibrio cellulolyticus strain CD2 16S ribosomal RNA, partial sequence	NR_025917	1464		35830

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM17959v2 assembly for Acetivibrio cellulolyticus CD2
BV-BRC: 509191.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	95.84	no
125439	motility	BacteriaNetⓘ	yes	80.44	no
125439	gram_stain	BacteriaNetⓘ	variable	67.32	no
125439	spore_formation	BacteriaNetⓘ	yes	88.22	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acetivibrio cellulolyticus CD2 CD2
NCBI: GCA_000179595.2

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	56.06	no
125438	anaerobic	anaerobicⓘ	yes	93.05	no
125438	aerobic	aerobicⓘ	no	95.40	yes
125438	spore-forming	spore-formingⓘ	yes	82.37	no
125438	thermophilic	thermophileⓘ	no	70.06	no
125438	flagellated	motile2+ⓘ	yes	77.77	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose-Degrading Anaerobic Bacteria.	Ishida K, Krabbe J, Meisinger PR, Shabuer G, Schieferdecker S, Cyrulies M, Tank C, Barnes E, Paetz C, Hertweck C.	Angew Chem Int Ed Engl	10.1002/anie.202503697	2025
Metabolism	Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system.	Dassa B, Borovok I, Lamed R, Henrissat B, Coutinho P, Hemme CL, Huang Y, Zhou J, Bayer EA.	BMC Genomics	10.1186/1471-2164-13-210	2012
Phylogeny	Molecular biological detection and characterization of Clostridium populations in municipal landfill sites.	Van Dyke MI, McCarthy AJ.	Appl Environ Microbiol	10.1128/aem.68.4.2049-2053.2002	2002
	Structure of CBM3b of the major cellulosomal scaffoldin subunit ScaA from Acetivibrio cellulolyticus.	Yaniv O, Halfon Y, Shimon LJ, Bayer EA, Lamed R, Frolow F.	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/s174430911104807x	2012
	Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin-dockerin interactions.	Hamberg Y, Ruimy-Israeli V, Dassa B, Barak Y, Lamed R, Cameron K, Fontes CM, Bayer EA, Fried DB.	PeerJ	10.7717/peerj.636	2014
Enzymology	The Electrosome: A Surface-Displayed Enzymatic Cascade in a Biofuel Cell's Anode and a High-Density Surface-Displayed Biocathodic Enzyme.	Szczupak A, Aizik D, Morais S, Vazana Y, Barak Y, Bayer EA, Alfonta L.	Nanomaterials (Basel)	10.3390/nano7070153	2017
Metabolism	Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum.	Ortiz de Ora L, Munoz-Gutierrez I, Bayer EA, Shoham Y, Lamed R, Borovok I.	Appl Environ Microbiol	10.1128/aem.03088-16	2017
Metabolism	A novel Acetivibrio cellulolyticus anchoring scaffoldin that bears divergent cohesins.	Xu Q, Barak Y, Kenig R, Shoham Y, Bayer EA, Lamed R.	J Bacteriol	10.1128/jb.186.17.5782-5789.2004	2004
Metabolism	The cellulosome system of Acetivibrio cellulolyticus includes a novel type of adaptor protein and a cell surface anchoring protein.	Xu Q, Gao W, Ding SY, Kenig R, Shoham Y, Bayer EA, Lamed R.	J Bacteriol	10.1128/jb.185.15.4548-4557.2003	2003
	A novel cellulosomal scaffoldin from Acetivibrio cellulolyticus that contains a family 9 glycosyl hydrolase.	Ding SY, Bayer EA, Steiner D, Shoham Y, Lamed R.	J Bacteriol	10.1128/jb.181.21.6720-6729.1999	1999
	Studies on Cellulose Hydrolysis by Acetivibrio cellulolyticus.	Mackenzie CR, Bilous D, Patel GB.	Appl Environ Microbiol	10.1128/aem.50.2.243-248.1985	1985
	Factors Involved in Hydrolysis of Microcrystalline Cellulose by Acetivibrio cellulolyticus.	Mackenzie CR, Patel GB, Bilous D.	Appl Environ Microbiol	10.1128/aem.53.2.304-308.1987	1987
	Conversion of Cellulose to Methane and Carbon Dioxide by Triculture of Acetivibrio cellulolyticus, Desulfovibrio sp., and Methanosarcina barkeri.	Laube VM, Martin SM.	Appl Environ Microbiol	10.1128/aem.42.3.413-420.1981	1981
Metabolism	Specialized cell surface structures in cellulolytic bacteria.	Lamed R, Naimark J, Morgenstern E, Bayer EA.	J Bacteriol	10.1128/jb.169.8.3792-3800.1987	1987
Metabolism	Contribution of a xylan-binding module to the degradation of a complex cellulosic substrate by designer cellulosomes.	Morais S, Barak Y, Caspi J, Hadar Y, Lamed R, Shoham Y, Wilson DB, Bayer EA.	Appl Environ Microbiol	10.1128/aem.00266-10	2010

References

#916	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1870
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#43408	Bareket Dassa, Ilya Borovok, Raphael Lamed, Bernard Henrissat, Pedro Coutinho, Christopher L Hemme, Yue Huang, Jizhong Zhou and Edward A Bayer: Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system. BMC Genomics 2012 ( DOI 10.1186/1471-2164-13-210 , PubMed 22646801 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68367	Automatically annotated from API 20A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acetivibrio cellulolyticus (DSM 1870, ATCC 33288, NRC 2248)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000179595

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

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