Strain identifier

BacDive ID: 17674

Type strain: Yes

Species: Acetivibrio cellulolyticus

Strain Designation: CD2

Strain history: <- G. B. Patel, National Research Council, Ottawa, Canada; CD2 <- G. B. Patel {1978}

NCBI tax ID(s): 509191 (strain), 35830 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 916

BacDive-ID: 17674

DSM-Number: 1870

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Acetivibrio cellulolyticus CD2 is an anaerobe, mesophilic bacterium that was isolated from sewage sludge.

NCBI tax id

NCBI tax idMatching level
35830species
509191strain

strain history

  • @ref: 916
  • history: <- G. B. Patel, National Research Council, Ottawa, Canada; CD2 <- G. B. Patel {1978}

doi: 10.13145/bacdive17674.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Oscillospiraceae
  • genus: Acetivibrio
  • species: Acetivibrio cellulolyticus
  • full scientific name: Acetivibrio cellulolyticus Patel et al. 1980

  • synonyms

    @refsynonym
    20215Acetivibrio cellulosolvens
    20215Hungateiclostridium cellulolyticum

@ref: 916

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Oscillospiraceae

genus: Acetivibrio

species: Acetivibrio cellulolyticus

full scientific name: Acetivibrio cellulolyticus Patel et al. 1980

strain designation: CD2

type strain: yes

Morphology

colony morphology

  • @ref: 916
  • incubation period: 8-14 days

Culture and growth conditions

culture medium

  • @ref: 916
  • name: ACETIVIBRIO CELLULOLYTICUS MEDIUM (DSMZ Medium 165)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/165
  • composition: Name: ACETIVIBRIO CELLULOLYTICUS MEDIUM (DSMZ Medium 165) Composition: Cellulose 2.997 g/l (optional) Cellobiose 2.997 g/l Na2CO3 1.4985 g/l (NH4)2SO4 0.44955 g/l K2HPO4 0.292208 g/l L-Cysteine HCl x H2O 0.24975 g/l Na2S x 9 H2O 0.24975 g/l KH2PO4 0.17982 g/l MgSO4 x 7 H2O 0.02997 g/l Nitrilotriacetic acid 0.014985 g/l NaCl 0.00999001 g/l MnSO4 x H2O 0.00499501 g/l CoSO4 x 7 H2O 0.0017982 g/l ZnSO4 x 7 H2O 0.0017982 g/l FeSO4 x 7 H2O 0.000999001 g/l CaCl2 x 2 H2O 0.000999001 g/l Sodium resazurin 0.0004995 g/l NiCl2 x 6 H2O 0.0002997 g/l AlK(SO4)2 x 12 H2O 0.0001998 g/l Na2MoO4 x 2 H2O 9.99001e-05 g/l H3BO3 9.99001e-05 g/l CuSO4 x 5 H2O 9.99001e-05 g/l Pyridoxine hydrochloride 9.99001e-05 g/l p-Aminobenzoic acid 4.995e-05 g/l (DL)-alpha-Lipoic acid 4.995e-05 g/l Thiamine HCl 4.995e-05 g/l Riboflavin 4.995e-05 g/l Nicotinic acid 4.995e-05 g/l Calcium D-(+)-pantothenate 4.995e-05 g/l Biotin 1.998e-05 g/l Folic acid 1.998e-05 g/l Na2WO4 x 2 H2O 3.996e-06 g/l Na2SeO3 x 5 H2O 2.997e-06 g/l Vitamin B12 9.99001e-07 g/l H2SO4 Distilled water

culture temp

  • @ref: 916
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 916
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4340862968cellulose+degradation
4340862968cellulose+hydrolysis
6836727082trehalose-builds acid from
6836762345L-rhamnose-builds acid from
6836730911sorbitol-builds acid from
6836716634raffinose-builds acid from
683676731melezitose-builds acid from
6836716024D-mannose-builds acid from
6836717057cellobiose+builds acid from
6836717754glycerol-builds acid from
683674853esculin+hydrolysis
683675291gelatin+hydrolysis
6836730849L-arabinose-builds acid from
6836765327D-xylose-builds acid from
6836717814salicin-builds acid from
6836717306maltose-builds acid from
6836717992sucrose-builds acid from
6836717716lactose-builds acid from
6836716899D-mannitol-builds acid from
6836717634D-glucose-builds acid from
6836716199urea-hydrolysis
6836727897tryptophan-energy source

metabolite production

  • @ref: 68367
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68367
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
43408cellulase+3.2.1.4
68367catalase-1.11.1.6
68367beta-glucosidase+3.2.1.21
68367gelatinase+
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRECAT
916----------++-+-------

Isolation, sampling and environmental information

isolation

  • @ref: 916
  • sample type: sewage sludge
  • geographic location: Ontario, Ottawa
  • country: Canada
  • origin.country: CAN
  • continent: North America

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Sewage sludge

taxonmaps

  • @ref: 69479
  • File name: preview.99_3417.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_196;96_1788;97_2140;98_2621;99_3417&stattab=map
  • Last taxonomy: Acetivibrio cellulolyticus
  • 16S sequence: NR_025917
  • Sequence Identity:
  • Total samples: 11531
  • soil counts: 600
  • aquatic counts: 545
  • animal counts: 10240
  • plant counts: 146

Safety information

risk assessment

  • @ref: 916
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acetivibrio cellulolyticus (ATCC 33288) 16S ribosomal RNA (16S rRNA)L355161464ena35830
916Hungateiclostridium cellulolyticum strain CD2 16S ribosomal RNA, partial sequenceNR_0259171464nuccore35830

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acetivibrio cellulolyticus CD2GCA_000179595scaffoldncbi509191
66792Acetivibrio cellulolyticus CD2509191.4wgspatric509191
66792Acetivibrio cellulolyticus CD22510461053draftimg509191

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes69.511no
flagellatedno63.807no
gram-positiveno65.417no
anaerobicyes99.697yes
aerobicno98.545yes
halophileno96.968no
spore-formingyes94.968no
thermophileno85.744yes
glucose-utilyes83.705no
glucose-fermentno50.971no

External links

@ref: 916

culture collection no.: DSM 1870, ATCC 33288, NRC 2248

straininfo link

  • @ref: 86665
  • straininfo: 10241

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
916Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1870)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1870
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
43408Bareket Dassa, Ilya Borovok, Raphael Lamed, Bernard Henrissat, Pedro Coutinho, Christopher L Hemme, Yue Huang, Jizhong Zhou and Edward A Bayer10.1186/1471-2164-13-210Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system22646801
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68367Automatically annotated from API 20A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86665Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10241.1StrainInfo: A central database for resolving microbial strain identifiers