Name: Acetobacter ghanensis 430A
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 22

Type strain:

Species: Acetobacter ghanensis

Strain Designation: 430A, R-29337

Culture col. no.: DSM 18895, LMG 23848

Strain history: <- L. De Vuyst, Vrije Univ. Brussels, Belgium; 430A <- N. Camu

NCBI tax ID(s): 1307907 (strain), 431306 (species)

Section

Acetobacter ghanensis 430A is a mesophilic, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from traditional heap fermentation of Ghanaian cocoa beans.

colony-forming
Gram-negative
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Acetobacter*
	Species	**Acetobacter ghanensis**
	Full Scientific Name (PNU)	Acetobacter ghanensis Cleenwerck et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #23237]	Gram stain	negative
[Ref.: #23237]	Cell length	1.5-2.5 µm
[Ref.: #23237]	Cell width	0.8 µm
[Ref.: #23237]	Cell shape	rod-shaped

[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #23237]	Colony size	0.8 mm
[Ref.: #23237]	Colony color	beige
[Ref.: #23237]	Colony shape	circular
[Ref.: #23237]	Incubation period	4 days
[Ref.: #23237]	Cultivation medium used	MYP agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7761]

Culture medium

MANNITOL-YEAST EXTRACT-PEPTONE (DSMZ Medium 1087)

[Ref.: #7761]

Culture medium growth

[Ref.: #7761]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #7761]

Culture medium composition

expand / minimize

[Ref.: #23237]

Culture medium

MYP agar

[Ref.: #23237]

Culture medium growth

[Ref.: #23237]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #7761]			growth	30 °C

[Ref.: #7761]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

[Ref.: #23237]	Name of tolerated compound	Ethanol
[Ref.: #23237]	Tolerance percentage	10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23237]	ethanol ChEBI	+	oxidation
[Ref.: #23237]	glycerol ChEBI	+	carbon source
[Ref.: #23237]	maltose ChEBI	-	carbon source
[Ref.: #23237]	methanol ChEBI	-	carbon source

	Metabolite production	Metabolite
[Ref.: #23237]		2-dehydro-D-gluconate ChEBI
[Ref.: #23237]		5-dehydro-D-gluconate ChEBI
[Ref.: #23237]		D-gluconate ChEBI

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7761]	Sample type/isolated from	traditional heap fermentation of Ghanaian cocoa beans
[Ref.: #7761]	Country	Ghana
[Ref.: #7761]	Country ISO 3 Code	GHA
[Ref.: #7761]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Food
#Host Body-Site	#Plant	#Fruit (Seed)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB665083 (>99% sequence identity) for Acetobacter from Microbeatlas

Aquatic Samples	428
Soil Samples	352
Animal Samples	2157
Plant Samples	309
Total Samples	3246

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7761]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7761]	Acetobacter ghanensis strain 430A 16S ribosomal RNA gene, partial sequence	EF030713	1442	ENA	431306 tax ID
[Ref.: #20218]	Acetobacter ghanensis gene for 16S rRNA, partial sequence, strain: LMG 23848	AB665083	1412	ENA	431306 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Acetobacter ghanensis LMG 23848	GCA_011516865	contig	GenBank	431306 tax ID	*
[Ref.: #66792]	Acetobacter ghanensis strain LMG 23848	431306.8	wgs	PATRIC	431306 tax ID	*
[Ref.: #66792]	Acetobacter ghanensis LMG 23848	2675903422	complete	JGI IMG/M	431306 tax ID	*

[Ref.: #7761]	GC-content	57.3 mol%
[Ref.: #23237]	GC-content	57.30 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Acetobacter ghanensis strain LMG 23848 PATRIC: 431306.5
[Ref.: #69480]	gram-positive	no	99.999	yes
[Ref.: #69480]	thermophile	no	99.631	no
[Ref.: #69480]	anaerobic	no	96.742	no
[Ref.: #69480]	spore-forming	no	99.998	yes
[Ref.: #69480]	motile	yes	72.247	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Acetobacter ghanensis strain LMG 23848 PATRIC: 431306.5
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #7761]

Culture collection no.

DSM 18895, LMG 23848

[Ref.: #20218]

Associated Passport(s) in StrainInfo

681623, 720493

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Assessment of the contribution of cocoa-derived strains of Acetobacter ghanensis and Acetobacter senegalensis to the cocoa bean fermentation process through a genomic approach.	Illeghems K, Pelicaen R, De Vuyst L, Weckx S	Food Microbiol	10.1016/j.fm.2016.03.013	2016	*
Metabolism	Oxidation of metabolites highlights the microbial interactions and role of Acetobacter pasteurianus during cocoa bean fermentation.	Moens F, Lefeber T, De Vuyst L	Appl Environ Microbiol	10.1128/AEM.03344-13	2014	*
Phylogeny	Acetobacter ghanensis sp. nov., a novel acetic acid bacterium isolated from traditional heap fermentations of Ghanaian cocoa beans.	Cleenwerck I, Camu N, Engelbeen K, De Winter T, Vandemeulebroecke K, De Vos P, De Vuyst L	Int J Syst Evol Microbiol	10.1099/ijs.0.64840-0	2007	*
Genetics	Application of comparative genomics of Acetobacter species facilitates genome-scale metabolic reconstruction of the Acetobacter ghanensis LMG 23848(T) and Acetobacter senegalensis 108B cocoa strains.	Pelicaen R, Weckx S, Gonze D, De Vuyst L	Front Microbiol	10.3389/fmicb.2022.1060160	2022	*

References

#7761

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18895

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23237

Ilse Cleenwerck, Nicholas Camu, Katrien Engelbeen, Tom De Winter, Katrien Vandemeulebroecke, Paul De Vos, Luc De Vuyst: Acetobacter ghanensis sp. nov., a novel acetic acid bacterium isolated from traditional heap fermentations of Ghanaian cocoa beans. IJSEM 57: 1647 - 1652 2007 ( DOI 10.1099/ijs.0.64840-0 , PubMed 17625210 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Acetobacter ghanensis strain LMG 23848

Acetobacter ghanensis strain LMG 23848

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