Strain identifier

BacDive ID: 22

Type strain: Yes

Species: Acetobacter ghanensis

Strain Designation: 430A, R-29337

Strain history: <- L. De Vuyst, Vrije Univ. Brussels, Belgium; 430A <- N. Camu

NCBI tax ID(s): 1307907 (strain), 431306 (species)

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General

@ref: 7761

BacDive-ID: 22

DSM-Number: 18895

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Acetobacter ghanensis 430A is a mesophilic, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from traditional heap fermentation of Ghanaian cocoa beans.

NCBI tax id

NCBI tax idMatching level
1307907strain
431306species

strain history

  • @ref: 7761
  • history: <- L. De Vuyst, Vrije Univ. Brussels, Belgium; 430A <- N. Camu

doi: 10.13145/bacdive22.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acetobacter
  • species: Acetobacter ghanensis
  • full scientific name: Acetobacter ghanensis Cleenwerck et al. 2007

@ref: 7761

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acetobacter

species: Acetobacter ghanensis

full scientific name: Acetobacter ghanensis Cleenwerck et al. 2007

strain designation: 430A, R-29337

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapeconfidence
23237negative1.5-2.5 µm0.8 µmrod-shaped
69480negative99.999

colony morphology

  • @ref: 23237
  • colony size: 0.8 mm
  • colony color: beige
  • colony shape: circular
  • incubation period: 4 days
  • medium used: MYP agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7761MANNITOL-YEAST EXTRACT-PEPTONE (DSMZ Medium 1087)yeshttps://mediadive.dsmz.de/medium/1087Name: MANNITOL-YEAST EXTRACT-PEPTONE (MYP) (DSMZ Medium 1087) Composition: D-Mannitol 25.0 g/l Agar 15.0 g/l Yeast extract 5.0 g/l Peptone 3.0 g/l Distilled water
23237MYP agaryes2.5 % (w/v) D-mannitol, 0.5 % (w/v) yeast extract and 0.3 % (w/v) bacteriological peptone (Oxoid)

culture temp

  • @ref: 7761
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

tolerance

  • @ref: 23237
  • compound: Ethanol
  • percentage: 10

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2323717306maltose-carbon source
2323717790methanol-carbon source
2323717754glycerol+carbon source
2323716236ethanol+oxidation

metabolite production

@refChebi-IDmetaboliteproduction
23237174265-dehydro-D-gluconateno
23237168082-dehydro-D-gluconateno
2323718391D-gluconateyes

Isolation, sampling and environmental information

isolation

  • @ref: 7761
  • sample type: traditional heap fermentation of Ghanaian cocoa beans
  • country: Ghana
  • origin.country: GHA
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Food
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1246.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_152;97_163;98_985;99_1246&stattab=map
  • Last taxonomy: Acetobacter
  • 16S sequence: AB665083
  • Sequence Identity:
  • Total samples: 3246
  • soil counts: 352
  • aquatic counts: 428
  • animal counts: 2157
  • plant counts: 309

Safety information

risk assessment

  • @ref: 7761
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acetobacter ghanensis gene for 16S rRNA, partial sequence, strain: LMG 23848AB6650831412ena431306
7761Acetobacter ghanensis strain 430A 16S ribosomal RNA gene, partial sequenceEF0307131442ena431306

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acetobacter ghanensis LMG 23848GCA_011516865contigncbi431306
66792Acetobacter ghanensis strain LMG 23848431306.8wgspatric431306
66792Acetobacter ghanensis LMG 238482675903422completeimg431306

GC content

@refGC-contentmethod
776157.3
2323757.30high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

  • trait: spore-forming
  • prediction: no
  • confidence: 100
  • training_data: no

External links

@ref: 7761

culture collection no.: DSM 18895, LMG 23848

straininfo link

  • @ref: 69706
  • straininfo: 292185

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17625210Acetobacter ghanensis sp. nov., a novel acetic acid bacterium isolated from traditional heap fermentations of Ghanaian cocoa beans.Cleenwerck I, Camu N, Engelbeen K, De Winter T, Vandemeulebroecke K, De Vos P, De Vuyst LInt J Syst Evol Microbiol10.1099/ijs.0.64840-02007Acetic Acid/*metabolism, Acetobacter/chemistry/*classification/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cacao/*microbiology, Catalase/analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Ethanol/metabolism, Fermentation, Food Microbiology, Genes, rRNA, Glucose/metabolism, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Oxidoreductases/analysis, Phylogeny, Polymerase Chain Reaction/methods, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic AcidMetabolism
Metabolism24413595Oxidation of metabolites highlights the microbial interactions and role of Acetobacter pasteurianus during cocoa bean fermentation.Moens F, Lefeber T, De Vuyst LAppl Environ Microbiol10.1128/AEM.03344-132014Acetobacter/growth & development/metabolism/*physiology, Cacao/*metabolism, Coculture Techniques, Culture Media/chemistry, Ethanol/metabolism, Fermentation, *Food Microbiology, Lactic Acid/metabolism, Lactobacillus fermentum/growth & development/metabolism, Mannitol/metabolism, *Microbial Interactions, Oxidation-Reduction, Saccharomyces cerevisiae/growth & development/metabolismBiotechnology
Metabolism27217361Assessment of the contribution of cocoa-derived strains of Acetobacter ghanensis and Acetobacter senegalensis to the cocoa bean fermentation process through a genomic approach.Illeghems K, Pelicaen R, De Vuyst L, Weckx SFood Microbiol10.1016/j.fm.2016.03.0132016Acetic Acid/metabolism, Acetobacter/enzymology/*genetics/physiology, Bacterial Proteins/genetics/metabolism, Cacao/metabolism/*microbiology, Fermentation, *Food Microbiology, Genome, Bacterial/*genetics, *Genomics, Lactic Acid/metabolism, NADP Transhydrogenases/genetics/metabolism, Phylogeny, Seeds/enzymology/metabolism/microbiology/physiology, Sequence Analysis, DNAGenetics
Genetics36504784Application of comparative genomics of Acetobacter species facilitates genome-scale metabolic reconstruction of the Acetobacter ghanensis LMG 23848(T) and Acetobacter senegalensis 108B cocoa strains.Pelicaen R, Weckx S, Gonze D, De Vuyst LFront Microbiol10.3389/fmicb.2022.10601602022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7761Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18895)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18895
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23237Ilse Cleenwerck, Nicholas Camu, Katrien Engelbeen, Tom De Winter, Katrien Vandemeulebroecke, Paul De Vos, Luc De Vuyst10.1099/ijs.0.64840-0Acetobacter ghanensis sp. nov., a novel acetic acid bacterium isolated from traditional heap fermentations of Ghanaian cocoa beansIJSEM 57: 1647-1652 200717625210
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69706Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID292185.1StrainInfo: A central database for resolving microbial strain identifiers