Acaricomes phytoseiuli (DSM 14247, CCUG 49701, NBRC 107788)

Name: Acaricomes phytoseiuli (DSM 14247, CCUG 49701, NBRC 107788)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7517

Type strain

Strain Designation CSC

Culture col. no. DSM 14247 CCUG 49701 NBRC 107788 CIP 109141 CSC 1 more

NCBI tax ID(s) 1120917 291968

Special Collections DSMZ CCUG JCM CIP Wink compendium Literature strains

Links

Acaricomes phytoseiuli CSC is an aerobe, mesophilic, Gram-positive animal pathogen that was isolated from predatory mite Phytoseiulus persimilis.

Gram-positive coccus-shaped aerobe mesophilic animal pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 132653

NBRC 107788,DSM 14247,CCUG 49701,JCM 14300,CIP 109141

@ref 20215

Last LPSN update 2025-12-16 09:48:36

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Acaricomes

Species Acaricomes phytoseiuli

Full scientific name Acaricomes phytoseiuli Pukall et al. 2006

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31628	positive	1-1.5 µm	0.5-0.8 µm	coccus-shaped
121819	positive			rod-shaped
125438					91.5
125438	positive				93.403
125439	positive				95.9
125439					93.8

Colony morphology

@ref	Colony color	Incubation period	Medium used
18380	Lemon yellow (1012)	10-14 days	ISP 2
18380	Lemon yellow (1012)	10-14 days	ISP 3
18380	Lemon yellow (1012)	10-14 days	ISP 4
18380	Lemon yellow (1012)	10-14 days	ISP 5
18380	Lemon yellow (1012)	10-14 days	ISP 6
18380	Lemon yellow (1012)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18380		ISP 2
18380		ISP 3
18380		ISP 4
18380		ISP 5
18380		ISP 6
18380		ISP 7

Pigmentation

31628

Productionyes

Multimedia

@ref:	66793
multimedia content:	EM_DSM_14247_1.jpg
multimedia.multimedia content:	EM_DSM_14247_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_14247_2.jpg
multimedia.multimedia content:	EM_DSM_14247_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_14247_3.jpg
multimedia.multimedia content:	EM_DSM_14247_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_14247_4.jpg
multimedia.multimedia content:	EM_DSM_14247_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_14247_5.jpg
multimedia.multimedia content:	EM_DSM_14247_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5270	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18380	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18380	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18380	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18380	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18380	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18380	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40126	MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
121819	CIP Medium 72	Medium recipe at CIP
121819	CIP Medium 10	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5270	positive	growth	25	mesophilic
18380	positive	optimum	25	mesophilic
31628	positive	growth	15-30	mesophilic
31628	positive	optimum	25	mesophilic
40126	positive	growth	25	mesophilic
58666	positive	growth	30	mesophilic
67770	positive	growth	25	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
31628	positive	optimum	06-08
31628	positive	growth	6-9.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
31628	aerobe
58666	aerobe
121819	obligate aerobe

Spore formation

@ref	Spore formation	Confidence
31628
125439		90.6

Observation

@ref	Observation
31628	aggregates in chains
67770	quinones: MK-10(H₂) (MMK-10(H₂)), MK-11(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
31628	23652 ChEBI	dextrin	+	carbon source
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
31628	28757 ChEBI	fructose	+	carbon source
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
31628	17234 ChEBI	glucose	+	carbon source
31628	29987 ChEBI	glutamate	+	carbon source
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
31628	17306 ChEBI	maltose	+	carbon source
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
31628	37684 ChEBI	mannose	+	carbon source
121819	17632 ChEBI	nitrate	-	reduction
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
121819	16301 ChEBI	nitrite	-	reduction
31628	17992 ChEBI	sucrose	+	carbon source
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121819	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
31628	catalase	+	1.11.1.6
121819	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121819	oxidase	-
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
121819	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18380	-	-	-	+	-	-	+	-	-	+	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18380	not determinedn.d.	+	+	+	+	+	-	-	+	-	+	+	-	-	-	+	+	-	-	-
121819	-	+	+	+	-	+	-	-	+	-	-	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host	#Arthropoda

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent	Geographic location
5270	predatory mite Phytoseiulus persimilis	Phytoseiulus persimilis	Netherlands	NLD	Europe
58666	From the predatory mite Phytoseiulus persimilis
67770	Predatory mite Phytoseiulus persimilis	Phytoseiulus persimilis
121819	Predatory mite, Phytoseiulus persimilis		Germany	DEU	Europe	Braunschweig

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
5270		1	Risk group (German classification) According to TRBA 466
121819		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM37624v1 assembly for Acaricomes phytoseiuli DSM 14247	contig	GCA_000376245	1120917.3	2517572146	1120917	66

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5270	Acaricomes phytoseiuli partial 16S rRNA gene, type strain DSM 14247T	AJ812213	1478		291968

GC content

@ref	GC-content (mol%)	Method
5270	57.7
67770	62.3	genome sequence analysis
67770	57.2-58.2	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Acaricomes phytoseiuli DSM 14247
NCBI: GCA_000376245

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	90.60	no
125439	motility	BacteriaNetⓘ	no	93.80	no
125439	gram_stain	BacteriaNetⓘ	positive	95.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	79.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acaricomes phytoseiuli DSM 14247
PATRIC: 1120917.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.40	yes
125438	anaerobic	anaerobicⓘ	no	97.76	yes
125438	spore-forming	spore-formingⓘ	no	82.61	yes
125438	aerobic	aerobicⓘ	yes	87.94	yes
125438	thermophilic	thermophileⓘ	no	96.50	yes
125438	flagellated	motile2+ⓘ	no	91.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	dBBQs: dataBase of Bacterial Quality scores.	Wanchai V, Patumcharoenpol P, Nookaew I, Ussery D.	BMC Bioinformatics	10.1186/s12859-017-1900-9	2017
	Novel bacterial pathogen Acaricomes phytoseiuli causes severe disease symptoms and histopathological changes in the predatory mite Phytoseiulus persimilis (Acari, Phytoseiidae).	Schutte C, Gols R, Kleespies RG, Poitevin O, Dicke M	J Invertebr Pathol	10.1016/j.jip.2008.03.006	2008
	Detection, Detrimental Effects, and Transmission Pathways of the Pathogenic Bacterium Acaricomes phytoseiuli in Commercial Predatory Mites.	Xie Z, Hoffmann AA, Zhang B, Xu X	Microbiol Spectr	10.1128/spectrum.02654-22	2022
Phylogeny	Acaricomes phytoseiuli gen. nov., sp. nov., isolated from the predatory mite Phytoseiulus persimilis.	Pukall R, Schumann P, Schutte C, Gols R, Dicke M	Int J Syst Evol Microbiol	10.1099/ijs.0.63930-0	2006

References

#5270	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14247
#18380	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#31628	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27913 (see below)
#40126	; Curators of the CIP;
#58666	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 49701
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#121819	Collection of Institut Pasteur ; Curators of the CIP; CIP 109141
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acaricomes phytoseiuli (DSM 14247, CCUG 49701, NBRC 107788)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000376245

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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