Escherichia coli K12 RV308 is a mesophilic prokaryote of the family Enterobacteriaceae.
mesophilic genome sequence| @ref 20215 |
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Last LPSN update
2026-02-09 11:18:50 |
| Domain Bacteria |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Enterobacterales |
| Family Enterobacteriaceae |
| Genus Escherichia |
| Species Escherichia coli |
| Full scientific name Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980) |
| Synonyms (1) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 5740 | LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) | Medium recipe at MediaDive  | Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 5740 | positive | growth | 37 | mesophilic |
| @ref | Oxygen tolerance | Confidence | |
|---|---|---|---|
| 125439 | aerobe | 90.4 |
| @ref | Spore formation | Confidence | |
|---|---|---|---|
| 125439 | 90.1 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68368 | 27613 ChEBI | amygdalin | - | fermentation | from API 20E |
| 68368 | 29016 ChEBI | arginine | - | hydrolysis | from API 20E |
| 68368 | 16947 ChEBI | citrate | - | assimilation | from API 20E |
| 68368 | 17634 ChEBI | D-glucose | + | fermentation | from API 20E |
| 68368 | 16899 ChEBI | D-mannitol | + | fermentation | from API 20E |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 68368 | 30849 ChEBI | L-arabinose | + | fermentation | from API 20E |
| 68368 | 62345 ChEBI | L-rhamnose | + | fermentation | from API 20E |
| 68368 | 25094 ChEBI | lysine | + | degradation | from API 20E |
| 68368 | 17268 ChEBI | myo-inositol | - | fermentation | from API 20E |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 68368 | 30911 ChEBI | sorbitol | + | fermentation | from API 20E |
| 68368 | 17992 ChEBI | sucrose | - | fermentation | from API 20E |
| 68368 | 27897 ChEBI | tryptophan | + | energy source | from API 20E |
| 68368 | 16199 ChEBI | urea | - | hydrolysis | from API 20E |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68368 | arginine dihydrolase | - | 3.5.3.6 | from API 20E |
| 68368 | cytochrome oxidase | - | 1.9.3.1 | from API 20E |
| 68368 | gelatinase | - | from API 20E | |
| 68368 | lysine decarboxylase | + | 4.1.1.18 | from API 20E |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 68368 | urease | - | 3.5.1.5 | from API 20E |
| @ref | ONPG | ADH (Arg) | LDC (Lys) | ODC | CIT | H2S productionH2S | URE | TDA (Trp) | IND | Acetoin production (Voges Proskauer test)VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX | Nitrite productionNO2 | Reduction to N2N2 | MotilityMOB | Growth on MacConkey mediumMAC | OF-O | OF-F | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 5740 | + | - | + | - | - | - | - | - | + | - | - | + | + | - | + | + | - | + | - | + | - | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | |
| 5740 | - | - | + | - | - | - | - | - | + | - | - | + | + | - | + | + | - | - | - | + | - | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. |
| @ref | Description | Assembly level | INSDC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|
| 66792 | EcRV308Chr assembly for Escherichia coli K-12 substr. RV308 | complete | GCA_000952955   | 2636415583  | 562 | 99.28 | |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Finished Genome Sequence of the Laboratory Strain Escherichia coli K-12 RV308 (ATCC 31608). | Krempl PM, Mairhofer J, Striedner G, Thallinger GG. | Genome Announc | 10.1128/genomea.00971-14 | 2014 | | |
| Production and Characterization of Novel Fabs Generated from Different Phage Display Libraries as Probes for Immunoassays for Gluten Detection in Food. | Garcia-Calvo E, Garcia-Garcia A, Rodriguez S, Takkinen K, Martin R, Garcia T. | Foods | 10.3390/foods12173274 | 2023 | | |
| Metabolism | Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli. | Gawin A, Ertesvag H, Hansen SAH, Malmo J, Brautaset T. | BMC Biotechnol | 10.1186/s12896-020-00615-0 | 2020 | |
| Dual UTR-A novel 5' untranslated region design for synthetic biology applications. | Balzer Le S, Onsager I, Lorentzen JA, Lale R. | Synth Biol (Oxf) | 10.1093/synbio/ysaa006 | 2020 | | |
| High-throughput microbioreactor provides a capable tool for early stage bioprocess development. | Fink M, Cserjan-Puschmann M, Reinisch D, Striedner G. | Sci Rep | 10.1038/s41598-021-81633-6 | 2021 | | |
| Enzymology | Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family. | Gawin A, Tietze L, Aarstad OA, Aachmann FL, Brautaset T, Ertesvag H. | Sci Rep | 10.1038/s41598-020-68789-3 | 2020 | |
| Metabolism | Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette. | Zwick F, Lale R, Valla S. | Microb Cell Fact | 10.1186/1475-2859-11-133 | 2012 | |
| Secretion of recombinant proteins from E. coli. | Kleiner-Grote GRM, Risse JM, Friehs K. | Eng Life Sci | 10.1002/elsc.201700200 | 2018 | | |
| Utilization of Multi-Immunization and Multiple Selection Strategies for Isolation of Hapten-Specific Antibodies from Recombinant Antibody Phage Display Libraries. | Tullila A, Nevanen TK. | Int J Mol Sci | 10.3390/ijms18061169 | 2017 | | |
| Metabolism | Evaluation of three industrial Escherichia coli strains in fed-batch cultivations during high-level SOD protein production. | Marisch K, Bayer K, Cserjan-Puschmann M, Luchner M, Striedner G. | Microb Cell Fact | 10.1186/1475-2859-12-58 | 2013 | |
| Reproducible biofilm cultivation of chemostat-grown Escherichia coli and investigation of bacterial adhesion on biomaterials using a non-constant-depth film fermenter. | Ludecke C, Jandt KD, Siegismund D, Kujau MJ, Zang E, Rettenmayr M, Bossert J, Roth M. | PLoS One | 10.1371/journal.pone.0084837 | 2014 | | |
| Metabolism | A comparative analysis of industrial Escherichia coli K-12 and B strains in high-glucose batch cultivations on process-, transcriptome- and proteome level. | Marisch K, Bayer K, Scharl T, Mairhofer J, Krempl PM, Hummel K, Razzazi-Fazeli E, Striedner G. | PLoS One | 10.1371/journal.pone.0070516 | 2013 | |
| Metabolism | Design and optimization of short DNA sequences that can be used as 5' fusion partners for high-level expression of heterologous genes in Escherichia coli. | Kucharova V, Skancke J, Brautaset T, Valla S. | Appl Environ Microbiol | 10.1128/aem.01676-13 | 2013 | |
| Metabolism | Genetic circuit performance under conditions relevant for industrial bioreactors. | Moser F, Broers NJ, Hartmans S, Tamsir A, Kerkman R, Roubos JA, Bovenberg R, Voigt CA. | ACS Synth Biol | 10.1021/sb3000832 | 2012 | |
| Identification of a New Phosphatase Enzyme Potentially Involved in the Sugar Phosphate Stress Response in Pseudomonas fluorescens. | Maleki S, Hrudikova R, Zotchev SB, Ertesvag H. | Appl Environ Microbiol | 10.1128/aem.02361-16 | 2017 | | |
| Metabolism | Broad-host-range plasmid pJB658 can be used for industrial-level production of a secreted host-toxic single-chain antibody fragment in Escherichia coli. | Sletta H, Nedal A, Aune TE, Hellebust H, Hakvag S, Aune R, Ellingsen TE, Valla S, Brautaset T. | Appl Environ Microbiol | 10.1128/aem.70.12.7033-7039.2004 | 2004 | |
| Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine beta-lactoglobulin allergen. | Niemi M, Janis J, Jylha S, Kallio JM, Hakulinen N, Laukkanen ML, Takkinen K, Rouvinen J. | Acta Crystallogr Sect F Struct Biol Cryst Commun | 10.1107/s174430910706160x | 2008 | | |
| Metabolism | Combinatorial mutagenesis and selection of improved signal sequences and their application for high-level production of translocated heterologous proteins in Escherichia coli. | Heggeset TM, Kucharova V, Naerdal I, Valla S, Sletta H, Ellingsen TE, Brautaset T. | Appl Environ Microbiol | 10.1128/aem.02407-12 | 2013 | |
| Oil degradation potential of microbial communities in water and sediment of Baltic Sea coastal area. | Miettinen H, Bomberg M, Nyyssonen M, Reunamo A, Jorgensen KS, Vikman M. | PLoS One | 10.1371/journal.pone.0218834 | 2019 | | |
| Cell water dynamics on multiple time scales. | Persson E, Halle B. | Proc Natl Acad Sci U S A | 10.1073/pnas.0709585105 | 2008 | | |
| Random mutagenesis of the PM promoter as a powerful strategy for improvement of recombinant-gene expression. | Bakke I, Berg L, Aune TE, Brautaset T, Sletta H, Tondervik A, Valla S. | Appl Environ Microbiol | 10.1128/aem.02315-08 | 2009 | | |
| Metabolism | The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli. | Sletta H, Tondervik A, Hakvag S, Aune TE, Nedal A, Aune R, Evensen G, Valla S, Ellingsen TE, Brautaset T. | Appl Environ Microbiol | 10.1128/aem.01804-06 | 2007 | |
| Metabolism | Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli. | Siurkus J, Neubauer P. | Microb Cell Fact | 10.1186/1475-2859-10-31 | 2011 | |
| Nitrate and ammonia as nitrogen sources for deep subsurface microorganisms. | Kutvonen H, Rajala P, Carpen L, Bomberg M. | Front Microbiol | 10.3389/fmicb.2015.01079 | 2015 | | |
| Microbiome composition and geochemical characteristics of deep subsurface high-pressure environment, Pyhäsalmi mine Finland. | Miettinen H, Kietavainen R, Sohlberg E, Numminen M, Ahonen L, Itavaara M. | Front Microbiol | 10.3389/fmicb.2015.01203 | 2015 | | |
| Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks. | Panula-Perala J, Siurkus J, Vasala A, Wilmanowski R, Casteleijn MG, Neubauer P. | Microb Cell Fact | 10.1186/1475-2859-7-31 | 2008 | | |
| Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures. | Siurkus J, Panula-Perala J, Horn U, Kraft M, Rimseliene R, Neubauer P. | Microb Cell Fact | 10.1186/1475-2859-9-35 | 2010 | | |
| Metabolism | Increased production of low molecular weight recombinant proteins in Escherichia coli. | Belagaje RM, Reams SG, Ly SC, Prouty WF. | Protein Sci | 10.1002/pro.5560060916 | 1997 | |
| CD4(+) T-cell-mediated antiviral protection of the upper respiratory tract in BALB/c mice following parenteral immunization with a recombinant respiratory syncytial virus G protein fragment. | Plotnicky-Gilquin H, Robert A, Chevalet L, Haeuw JF, Beck A, Bonnefoy JY, Brandt C, Siegrist CA, Nguyen TN, Power UF. | J Virol | 10.1128/jvi.74.8.3455-3463.2000 | 2000 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive4897.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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