Name: Escherichia coli K12 HB101
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 4434

Type strain:

Species: Escherichia coli

Strain Designation: K12 HB101, HB101

Culture col. no.: DSM 1607, CIP 102400, ATCC 33694, NCIMB 11865, CRBIP 19.4

Strain history: CIP <- 1986, Boyer, Paris, France: strain HB101

NCBI tax ID(s): 562 (species)

SI-ID 40628

ATCC 33694, CIP 102400, DSM 1607, NCIB 11865, NCIMB 11865, KCTC 1467, NCIM 2924, CECT 670, NCTC 50237

Special Collections

Other strains from Escherichia coli

Section

Escherichia coli K12 HB101 is a facultative aerobe, Gram-negative, rod-shaped bacterium that has multiple antibiotic resistances and was isolated from freshwater .

antibiotic resistance
Gram-negative
rod-shaped
facultative aerobe
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Escherichia*
	Species	**Escherichia coli**
	Full Scientific Name (LPSN)	Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

Synonym

"Bacillus coli"

Information on morphological and physiological properties Morphology

[Ref.: #31279]	Gram stain	negative
[Ref.: #118698]	Gram stain	negative

[Ref.: #31279]	Cell length	2.85 µm

[Ref.: #31279]	Cell width	0.65 µm

[Ref.: #31279]	Cell shape	rod-shaped
[Ref.: #118698]	Cell shape	rod-shaped

[Ref.: #31279]	Motility	no
[Ref.: #118698]	Motility	yes

[Ref.: #31279]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #829]

Culture medium

ENB MEDIUM (DSMZ Medium 237)

[Ref.: #829]

Culture medium growth

[Ref.: #829]

Culture medium link

Medium recipe at MediaDive

[Ref.: #829]

Culture medium composition

expand / minimize

[Ref.: #829]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #829]

Culture medium growth

[Ref.: #829]

Culture medium link

Medium recipe at MediaDive

[Ref.: #829]

Culture medium composition

expand / minimize

[Ref.: #37023]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37023]

Culture medium growth

[Ref.: #37023]

Culture medium composition

expand / minimize

[Ref.: #118698]

Culture medium

CIP Medium 72

[Ref.: #118698]

Culture medium growth

[Ref.: #118698]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #829]		growth	37 °C
[Ref.: #31279]		growth	04-37 °C
[Ref.: #31279]		optimum	30 °C
[Ref.: #37023]		growth	30 °C
[Ref.: #118698]		growth	10-41 °C
[Ref.: #118698]	no	growth	5 °C

	pH	Kind of pH		pH
[Ref.: #31279]			optimum	8.5
[Ref.: #31279]			growth	06-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31279]	Oxygen tolerance	facultative aerobe
[Ref.: #118698]	Oxygen tolerance	facultative anaerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Reference

[Ref.: #829]
Mueller-Hinton Agar
37
1
aerob
yes
Inhibition zone diameter in mm
10
resistant0
28
42
38-40
30
40
>50
>50
46
34-36
30
32
32
14-16
14-16
resistant0
>50
>50
20
38-40
>50
12
20-22
30
26-28
30
>50
20
44
>50
8
10
8
10
40

[Ref.: #829]
M 1



yes
Inhibition zone diameter in mm
8
resistant0
34
36
34
30
36-38
50
50
48-50
38
36-38
14
20-22
10-12
14
resistant0
44
44-46
10-12
36-38
44-46
10
12
34-36
12-14
32-34
48-50
resistant0
42-44
42-44
resistant0
resistant0
8
resistant0
36

[Ref.: #829]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
6
resistant0
28
38
34
22
34
50
46
42
34
36-38
26
28
10
12
resistant0
46
44
18
34-36
44
8
20
26
22
30
44
10
48-50
46
8
resistant0
8
resistant0
34-36

[Ref.: #829]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
6
resistant0
28
38
34
22
34
50
46
42
34
36-38
26
28
10
12
resistant0
46
44
18
34-36
44
8
20
26
22
30
44
10
48-50
46
8
resistant0
8
resistant0
34-36

Only first 15 entries are displayed. Click here to see all.

[Ref.: #31279]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31279]		NaCl		growth	0.5-10	%
[Ref.: #31279]		NaCl		optimum	5.25	%

[Ref.: #31279]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31279]	acetate ChEBI	+	carbon source
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #31279]	citrate ChEBI	+	carbon source
[Ref.: #118698]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #118698]	esculin ChEBI	+	hydrolysis
[Ref.: #31279]	fructose ChEBI	+	carbon source
[Ref.: #31279]	gelatin ChEBI	+	carbon source
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #31279]	glucose ChEBI	+	carbon source
[Ref.: #118698]	glucose ChEBI	+	fermentation
[Ref.: #31279]	glutamate ChEBI	+	carbon source
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #31279]	lactate ChEBI	+	carbon source
[Ref.: #118698]	lactose ChEBI	-	fermentation
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #31279]	malate ChEBI	+	carbon source
[Ref.: #118698]	malonate ChEBI	-	assimilation
[Ref.: #118698]	mannitol ChEBI	-	fermentation
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #118698]	nitrate ChEBI	+	reduction
[Ref.: #118698]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #31279]	pyruvate ChEBI	+	carbon source
[Ref.: #118698]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #31279]	succinate ChEBI	+	carbon source
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #118698]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite	Production
[Ref.: #118698]	indole ChEBI	yes
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #118698]	acetoin ChEBI	-
[Ref.: #118698]	glucose ChEBI		+
[Ref.: #68368]	acetoin ChEBI	-			* from API 20E
[Ref.: #68368]	indole ChEBI			+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118698]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #118698]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31279]	catalase	+	1.11.1.6
[Ref.: #118698]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31279]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #31279]	gelatinase	+
[Ref.: #118698]	gelatinase	-
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118698]	lysine decarboxylase	+	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118698]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #118698]	oxidase	-
[Ref.: #118698]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118698]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #118698]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #829]	16993	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	16992	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	16991	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	16990	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	16989	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118698]	220	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118698]	7057	-	+	-	-	-	+	-	-	-	-	+	+	+	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #31279]	Sample type/isolated from	freshwater (river, lake, pond)
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Pond (small)
#Environmental	#Aquatic	#River (Creek)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #829]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118698]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

[Ref.: #31279]

GC-content

66.2 mol%

Availability in culture collections External links

[Ref.: #829]

Culture collection no.

DSM 1607, CIP 102400, ATCC 33694, NCIMB 11865, CRBIP 19.4

[Ref.: #73924]

SI-ID 40628

References

#829	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1607

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31279

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27599 (see below)

#37023 ; Curators of the CIP;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#73924

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40628.1 )

#118698 Collection of Institut Pasteur ; Curators of the CIP; CIP 102400

#27599

IJSEM 1597 2005 ( DOI 10.1099/ijs.0.63195-0 , PubMed 16014488 )

* These data were automatically processed and therefore are not curated

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