Escherichia coli HB101 | DSM 1607, K12 HB101

Strain identifier

BacDive ID: 4434

Type strain:

Species: Escherichia coli

Strain Designation: HB101

Culture col. no.: DSM 1607, K12 HB101

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Escherichia coli HB101 is a facultative aerobe, mesophilic, gram-negative bacterium that has multiple antibiotic resistances and was isolated from freshwater .

antibiotic resistance
gram-negative
rod-shaped
facultative aerobe
mesophilic
Bacteria

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	10-09-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Proteobacteria
	Class	Gammaproteobacteria
	Order	Enterobacterales
	Family	Enterobacteriaceae
	Genus	Escherichia
	Species	Escherichia coli
	Full Scientific Name (PNU)	Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

Synonym

Bacillus coli

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #31279]	Gram stain	negative
[Ref.: #31279]	Cell length	2.85 µm
[Ref.: #31279]	Cell width	0.65 µm
[Ref.: #31279]	Cell shape	rod-shaped
[Ref.: #31279]	Motility	no

	Enzyme	Enzyme activity	EC number
[Ref.: #31279]	catalase	+	1.11.1.6
[Ref.: #31279]	cytochrome oxidase	+	1.9.3.1
[Ref.: #31279]	gelatinase	+

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31279]		NaCl		growth	0.5-10	%
[Ref.: #31279]		NaCl		optimum	5.25	%

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #829]	6746	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	6745	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	6744	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	6743	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	6742	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Reference

[Ref.: #829]
Mueller-Hinton Agar
37
1
aerob
yes
Inhibition zone diameter in mm
10
resistant0
28
42
38-40
30
40
>50
>50
46
34-36
30
32
32
14-16
14-16
resistant0
>50
>50
20
38-40
>50
12
20-22
30
26-28
30
>50
20
44
>50
8
10
8
10
40

[Ref.: #829]
M 1



yes
Inhibition zone diameter in mm
8
resistant0
34
36
34
30
36-38
50
50
48-50
38
36-38
14
20-22
10-12
14
resistant0
44
44-46
10-12
36-38
44-46
10
12
34-36
12-14
32-34
48-50
resistant0
42-44
42-44
resistant0
resistant0
8
resistant0
36

[Ref.: #829]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
6
resistant0
28
38
34
22
34
50
46
42
34
36-38
26
28
10
12
resistant0
46
44
18
34-36
44
8
20
26
22
30
44
10
48-50
46
8
resistant0
8
resistant0
34-36

Only first 15 entries are displayed. Click here to see all.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31279]	acetate ChEBI	+	carbon source
[Ref.: #31279]	citrate ChEBI	+	carbon source
[Ref.: #31279]	fructose ChEBI	+	carbon source
[Ref.: #31279]	gelatin ChEBI	+	carbon source
[Ref.: #31279]	glucose ChEBI	+	carbon source
[Ref.: #31279]	glutamate ChEBI	+	carbon source
[Ref.: #31279]	lactate ChEBI	+	carbon source
[Ref.: #31279]	malate ChEBI	+	carbon source
[Ref.: #31279]	pyruvate ChEBI	+	carbon source
[Ref.: #31279]	succinate ChEBI	+	carbon source

[Ref.: #31279]

Observation

aggregates in clumps

[Ref.: #31279]

Oxygen tolerance

facultative aerobe

[Ref.: #31279]

Ability of spore formation

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #829]

Culture medium

ENB MEDIUM (DSMZ Medium 237), 37°C

[Ref.: #829]

Culture medium growth

[Ref.: #829]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #829]

Culture medium

Nutrient agar (NA)

[Ref.: #829]

Culture medium growth

[Ref.: #829]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #37023]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37023]

Culture medium growth

[Ref.: #37023]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #829]	growth	37 ̊C
[Ref.: #31279]	growth	04-37 ̊C
[Ref.: #31279]	optimum	30 ̊C
[Ref.: #37023]	growth	30 ̊C

[Ref.: #829]	Temperature range	mesophilic
[Ref.: #31279]	Temperature range	mesophilic
[Ref.: #37023]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31279]			optimum	8.5
[Ref.: #31279]			growth	06-11

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #31279]

Sample type/isolated from

freshwater (river, lake, pond)

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Pond (small)
#Environmental	#Aquatic	#River (Creek)

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #829]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #31279]

GC-content

66.2 mol%

Sequence information:

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #31279]	GenBank/EMBL/DDBJ	Alpha proteobacterium ML6 16S rRNA gene, type strain ML6T	AJ315682 GenBank	16S	1435	164332 tax ID

Availability in culture collectionsStrain availability

[Ref.: #829]

Culture collection no.

DSM 1607, K12 HB101

[Ref.: #829]	Strain history	<- Schering AG
[Ref.: #37023]	Strain history	1986, Boyer, Paris, France: strain HB101

[Ref.: #20218]

Associated Passport(s) in StrainInfo

150799, 157435

References

#829	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1607

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31279

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 ) - originally annotated from #27599 (see below)

#37023 ; Curators of the CIP;

#27599

IJSEM 1597 2005 ( DOI 10.1099/ijs.0.63195-0 )

* These References are textmined

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