Name: Escherichia coli K12 HB101
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4434

Type strain:

Species: Escherichia coli

Strain Designation: K12 HB101, HB101

Culture col. no.: DSM 1607

Strain history: 1986, Boyer, Paris, France: strain HB101

NCBI tax ID(s): 562 (species)

Other strains from Escherichia coli

Section

Escherichia coli K12 HB101 is a facultative aerobe, mesophilic, Gram-negative bacterium that has multiple antibiotic resistances and was isolated from freshwater .

antibiotic resistance
Gram-negative
rod-shaped
facultative aerobe
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	25-08-2022 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Pseudomonadota
	Class	Gammaproteobacteria
	Order	Enterobacterales
	Family	Enterobacteriaceae
	Genus	Escherichia
	Species	Escherichia coli
	Full Scientific Name (PNU)	Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

Synonym

Bacillus coli

Information on morphological and physiological properties Morphology

[Ref.: #31279]	Gram stain	negative
[Ref.: #31279]	Cell length	2.85 µm
[Ref.: #31279]	Cell width	0.65 µm
[Ref.: #31279]	Cell shape	rod-shaped
[Ref.: #31279]	Motility	no

[Ref.: #31279]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #829]

Culture medium

ENB MEDIUM (DSMZ Medium 237)

[Ref.: #829]

Culture medium growth

[Ref.: #829]

Culture medium link

Medium recipe at MediaDive

[Ref.: #829]

Culture medium composition

expand / minimize

[Ref.: #829]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #829]

Culture medium growth

[Ref.: #829]

Culture medium link

Medium recipe at MediaDive

[Ref.: #829]

Culture medium composition

expand / minimize

[Ref.: #37023]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37023]

Culture medium growth

[Ref.: #37023]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #829]	growth	37 °C
[Ref.: #31279]	growth	04-37 °C
[Ref.: #31279]	optimum	30 °C
[Ref.: #37023]	growth	30 °C

[Ref.: #829]	Temperature range	mesophilic
[Ref.: #31279]	Temperature range	mesophilic
[Ref.: #37023]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31279]			optimum	8.5
[Ref.: #31279]			growth	06-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31279]

Oxygen tolerance

facultative aerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Reference

[Ref.: #829]
Mueller-Hinton Agar
37
1
aerob
yes
Inhibition zone diameter in mm
10
resistant0
28
42
38-40
30
40
>50
>50
46
34-36
30
32
32
14-16
14-16
resistant0
>50
>50
20
38-40
>50
12
20-22
30
26-28
30
>50
20
44
>50
8
10
8
10
40

[Ref.: #829]
M 1



yes
Inhibition zone diameter in mm
8
resistant0
34
36
34
30
36-38
50
50
48-50
38
36-38
14
20-22
10-12
14
resistant0
44
44-46
10-12
36-38
44-46
10
12
34-36
12-14
32-34
48-50
resistant0
42-44
42-44
resistant0
resistant0
8
resistant0
36

[Ref.: #829]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
6
resistant0
28
38
34
22
34
50
46
42
34
36-38
26
28
10
12
resistant0
46
44
18
34-36
44
8
20
26
22
30
44
10
48-50
46
8
resistant0
8
resistant0
34-36

[Ref.: #829]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
6
resistant0
28
38
34
22
34
50
46
42
34
36-38
26
28
10
12
resistant0
46
44
18
34-36
44
8
20
26
22
30
44
10
48-50
46
8
resistant0
8
resistant0
34-36

Only first 15 entries are displayed. Click here to see all.

[Ref.: #31279]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31279]		NaCl		growth	0.5-10	%
[Ref.: #31279]		NaCl		optimum	5.25	%

[Ref.: #31279]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31279]	acetate ChEBI	+	carbon source
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #31279]	citrate ChEBI	+	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #31279]	fructose ChEBI	+	carbon source
[Ref.: #31279]	gelatin ChEBI	+	carbon source
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #31279]	glucose ChEBI	+	carbon source
[Ref.: #31279]	glutamate ChEBI	+	carbon source
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #31279]	lactate ChEBI	+	carbon source
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #31279]	malate ChEBI	+	carbon source
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #31279]	pyruvate ChEBI	+	carbon source
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #31279]	succinate ChEBI	+	carbon source
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68368]	indole ChEBI	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #31279]	catalase	+	1.11.1.6
[Ref.: #31279]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #31279]	gelatinase	+
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #829]	11159	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	11158	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	11157	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	11156	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	11155	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #31279]

Sample type/isolated from

freshwater (river, lake, pond)

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Pond (small)
#Environmental	#Aquatic	#River (Creek)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #829]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

[Ref.: #31279]

GC-content

66.2 mol%

Availability in culture collections External links

[Ref.: #829]

Culture collection no.

DSM 1607

[Ref.: #20218]

Associated Passport(s) in StrainInfo

150799, 157435

References

#829	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1607

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31279

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27599 (see below)

#37023 ; Curators of the CIP;

#68368

Automatically annotated from API 20E .

#27599

IJSEM 1597 2005 ( DOI 10.1099/ijs.0.63195-0 , PubMed 16014488 )

* These data were automatically processed and therefore are not curated

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