Name: Escherichia coli K12 HB101
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4434

Type strain:

Species: Escherichia coli

Strain Designation: K12 HB101, HB101

Culture col. no.: DSM 1607

Strain history: 1986, Boyer, Paris, France: strain HB101

NCBI tax ID(s): 562 (species)

Other strains from Escherichia coli

Section

Escherichia coli K12 HB101 is a facultative aerobe, mesophilic, Gram-negative bacterium that has multiple antibiotic resistances and was isolated from freshwater .

antibiotic resistance
Gram-negative
rod-shaped
facultative aerobe
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Escherichia*
	Species	**Escherichia coli**
	Full Scientific Name (PNU)	Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

Synonym

"Bacillus coli"

Information on morphological and physiological properties Morphology

[Ref.: #31279]	Gram stain	negative
[Ref.: #31279]	Cell length	2.85 µm
[Ref.: #31279]	Cell width	0.65 µm
[Ref.: #31279]	Cell shape	rod-shaped
[Ref.: #31279]	Motility	no

[Ref.: #31279]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #829]

Culture medium

ENB MEDIUM (DSMZ Medium 237)

[Ref.: #829]

Culture medium growth

[Ref.: #829]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #829]

Culture medium composition

expand / minimize

[Ref.: #829]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #829]

Culture medium growth

[Ref.: #829]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #829]

Culture medium composition

expand / minimize

[Ref.: #37023]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37023]

Culture medium growth

[Ref.: #37023]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #829]	growth	37 °C
[Ref.: #31279]	growth	04-37 °C
[Ref.: #31279]	optimum	30 °C
[Ref.: #37023]	growth	30 °C

[Ref.: #829]	Temperature range	mesophilic
[Ref.: #31279]	Temperature range	mesophilic
[Ref.: #37023]	Temperature range	mesophilic

	pH	Kind of pH		pH	pH range
[Ref.: #31279]			optimum	8.5
[Ref.: #31279]			growth	06-11	alkaliphile

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31279]

Oxygen tolerance

facultative aerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Reference

[Ref.: #829]
Mueller-Hinton Agar
37
1
aerob
yes
Inhibition zone diameter in mm
10
resistant0
28
42
38-40
30
40
>50
>50
46
34-36
30
32
32
14-16
14-16
resistant0
>50
>50
20
38-40
>50
12
20-22
30
26-28
30
>50
20
44
>50
8
10
8
10
40

[Ref.: #829]
M 1



yes
Inhibition zone diameter in mm
8
resistant0
34
36
34
30
36-38
50
50
48-50
38
36-38
14
20-22
10-12
14
resistant0
44
44-46
10-12
36-38
44-46
10
12
34-36
12-14
32-34
48-50
resistant0
42-44
42-44
resistant0
resistant0
8
resistant0
36

[Ref.: #829]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
6
resistant0
28
38
34
22
34
50
46
42
34
36-38
26
28
10
12
resistant0
46
44
18
34-36
44
8
20
26
22
30
44
10
48-50
46
8
resistant0
8
resistant0
34-36

[Ref.: #829]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
6
resistant0
28
38
34
22
34
50
46
42
34
36-38
26
28
10
12
resistant0
46
44
18
34-36
44
8
20
26
22
30
44
10
48-50
46
8
resistant0
8
resistant0
34-36

Only first 15 entries are displayed. Click here to see all.

[Ref.: #31279]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31279]		NaCl		growth	0.5-10	%
[Ref.: #31279]		NaCl		optimum	5.25	%

[Ref.: #31279]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31279]	acetate ChEBI	+	carbon source
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #31279]	citrate ChEBI	+	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #31279]	fructose ChEBI	+	carbon source
[Ref.: #31279]	gelatin ChEBI	+	carbon source
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #31279]	glucose ChEBI	+	carbon source
[Ref.: #31279]	glutamate ChEBI	+	carbon source
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #31279]	lactate ChEBI	+	carbon source
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #31279]	malate ChEBI	+	carbon source
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #31279]	pyruvate ChEBI	+	carbon source
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #31279]	succinate ChEBI	+	carbon source
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68368]	indole ChEBI	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #31279]	catalase	+	1.11.1.6
[Ref.: #31279]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #31279]	gelatinase	+
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #829]	14399	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	14398	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	14397	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	14396	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #829]	14395	+	-	+	-	-	-	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #31279]	Sample type/isolated from	freshwater (river, lake, pond)
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Pond (small)
#Environmental	#Aquatic	#River (Creek)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #829]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

[Ref.: #31279]

GC-content

66.2 mol%

Availability in culture collections External links

[Ref.: #829]

Culture collection no.

DSM 1607

[Ref.: #20218]

Associated Passport(s) in StrainInfo

150799, 157435

References

#829	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1607

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31279

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27599 (see below)

#37023 ; Curators of the CIP;

#68368

Automatically annotated from API 20E .

#27599

IJSEM 1597 2005 ( DOI 10.1099/ijs.0.63195-0 , PubMed 16014488 )

* These data were automatically processed and therefore are not curated

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