Escherichia coli K12 HB101 is a facultative aerobe, mesophilic, Gram-negative prokaryote that has multiple antibiotic resistances and was isolated from freshwater .
antibiotic resistance Gram-negative rod-shaped facultative aerobe mesophilic
SI-ID 40628
| @ref 20215 |
|
Last LPSN update
2025-12-16 09:47:49 |
| Domain Pseudomonadati |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Enterobacterales |
| Family Enterobacteriaceae |
| Genus Escherichia |
| Species Escherichia coli |
| Full scientific name Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980) |
| Synonyms (1) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 829 | ENB MEDIUM (DSMZ Medium 237) | Medium recipe at MediaDive  | Name: ENB MEDIUM (DSMZ Medium 237) Composition: Agar 15.0 g/l Nutrient broth 8.0 g/l NaCl 5.0 g/l Peptone 5.0 g/l K2HPO4 3.5 g/l KH2PO4 1.5 g/l Glucose 1.0 g/l Distilled water | ||
| 829 | NUTRIENT AGAR (DSMZ Medium 1) | Medium recipe at MediaDive | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water | ||
| 37023 | MEDIUM 72- for trypto casein soja agar | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |||
| 118698 | CIP Medium 72 | Medium recipe at CIP |
| Test 1 | Test 2 | Test 3 | Test 4 | |
|---|---|---|---|---|
| @ref | 829 | 829 | 829 | 829 |
| Medium | Mueller-Hinton Agar | M 1 | Mueller-Hinton Agar | Mueller-Hinton Agar |
| Manual annotation | 1 | 1 | 1 | 1 |
| Inhibition zone diameter in mm | Inhibition zone diameter in mm | Inhibition zone diameter in mm | Inhibition zone diameter in mm | |
| Amikacin 30µg (disc) | 32 | 20-22 | 28 | 28 |
| Ampicillin 10µg (disc) | 28 | 34 | 28 | 28 |
| Aztreonam 30µg (disc) | >50 | 50 | 46 | 46 |
| Bacitracin 10Unit | 12 | 10 | 8 | 8 |
| Cefalotin 30µg (disc) | 30 | 30 | 22 | 22 |
| Cefazolin 30µg (disc) | 40 | 36-38 | 34 | 34 |
| Cefotaxime 30µg (disc) | >50 | 50 | 50 | 50 |
| Ceftriaxone 30µg (disc) | >50 | 48-50 | 44 | 44 |
| Chloramphenicol 30µg (disc) | 30 | 36-38 | 36-38 | 36-38 |
| Clindamycin 10µg (disc) | 20 | 0 | 10 | 10 |
| Colistin 10µg (disc) | 20 | 10-12 | 18 | 18 |
| Doxycycline 30µg (disc) | 30 | 32-34 | 30 | 30 |
| Erythromycin 15µg (disc) | 14-16 | 14 | 12 | 12 |
| Fosfomycin 50µg (disc) | 44 | 42-44 | 48-50 | 48-50 |
| Gentamycin 10µg (disc) | ||||
| Imipenem 10µg (disc) | 46 | 48-50 | 42 | 42 |
| Kanamycin 30µg (disc) | 30 | 34-36 | 26 | 26 |
| Lincomycin 15µg (disc) | 0 | 0 | 0 | 0 |
| Linezolid 10µg (disc) | 8 | 0 | 8 | 8 |
| Mezlocillin 30µg (disc) | 38-40 | 34 | 34 | 34 |
| Moxifloxacin 5µg (disc) | >50 | 42-44 | 46 | 46 |
| Neomycin 30µg (disc) | 26-28 | 12-14 | 22 | 22 |
| Nitrofurantoin 100µg (disc) | >50 | 44-46 | 44 | 44 |
| Norfloxacin 10µg (disc) | >50 | 44-46 | 44 | 44 |
| Nystatin 100Unit | 10 | 0 | 0 | 0 |
| Ofloxacin 5µg (disc) | >50 | 44 | 46 | 46 |
| Oxacillin 5µg (disc) | 0 | 0 | 0 | 0 |
| Penicillin G 6µg (disc) | 10 | 8 | 6 | 6 |
| Pipemidic acid 20µg (disc) | 38-40 | 36-38 | 34-36 | 34-36 |
| Piperacillin/Tazobactam 40µg (disc) | 40 | 36 | 34-36 | 34-36 |
| Polymyxin B 300Unit | 20-22 | 12 | 20 | 20 |
| Quinupristin/Dalfopristin 15µg (disc) | 8 | 8 | 8 | 8 |
| Teicoplanin 30µg (disc) | 10 | 0 | 0 | 0 |
| Tetracycline 30µg (disc) | 34-36 | 38 | 34 | 34 |
| Ticarcillin 75µg (disc) | 42 | 36 | 38 | 38 |
| Vancomycin 30µg (disc) | 14-16 | 10-12 | 10 | 10 |
| 31279 | Spore formationno |
| 31279 | Observationaggregates in clumps |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 31279 | 30089 ChEBI | acetate | + | carbon source | |
| 68368 | 27613 ChEBI | amygdalin | - | fermentation | from API 20E |
| 68368 | 29016 ChEBI | arginine | - | hydrolysis | from API 20E |
| 31279 | 16947 ChEBI | citrate | + | carbon source | |
| 118698 | 16947 ChEBI | citrate | - | carbon source | |
| 68368 | 16947 ChEBI | citrate | - | assimilation | from API 20E |
| 68368 | 17634 ChEBI | D-glucose | + | fermentation | from API 20E |
| 68368 | 16899 ChEBI | D-mannitol | - | fermentation | from API 20E |
| 118698 | 4853 ChEBI | esculin | + | hydrolysis | |
| 31279 | 28757 ChEBI | fructose | + | carbon source | |
| 31279 | 5291 ChEBI | gelatin | + | carbon source | |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 31279 | 17234 ChEBI | glucose | + | carbon source | |
| 118698 | 17234 ChEBI | glucose | + | fermentation | |
| 31279 | 29987 ChEBI | glutamate | + | carbon source | |
| 68368 | 30849 ChEBI | L-arabinose | - | fermentation | from API 20E |
| 68368 | 62345 ChEBI | L-rhamnose | + | fermentation | from API 20E |
| 31279 | 24996 ChEBI | lactate | + | carbon source | |
| 118698 | 17716 ChEBI | lactose | - | fermentation | |
| 68368 | 25094 ChEBI | lysine | + | degradation | from API 20E |
| 31279 | 25115 ChEBI | malate | + | carbon source | |
| 118698 | 15792 ChEBI | malonate | - | assimilation | |
| 118698 | 29864 ChEBI | mannitol | - | fermentation | |
| 68368 | 28053 ChEBI | melibiose | - | fermentation | from API 20E |
| 68368 | 17268 ChEBI | myo-inositol | - | fermentation | from API 20E |
| 118698 | 17632 ChEBI | nitrate | + | reduction | |
| 118698 | 16301 ChEBI | nitrite | - | reduction | |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 31279 | 15361 ChEBI | pyruvate | + | carbon source | |
| 118698 | 132112 ChEBI | sodium thiosulfate | - | builds gas from | |
| 68368 | 30911 ChEBI | sorbitol | + | fermentation | from API 20E |
| 31279 | 30031 ChEBI | succinate | + | carbon source | |
| 68368 | 17992 ChEBI | sucrose | - | fermentation | from API 20E |
| 68368 | 27897 ChEBI | tryptophan | + | energy source | from API 20E |
| 68368 | 16199 ChEBI | urea | - | hydrolysis | from API 20E |
| @ref | ChEBI | Metabolite | Is resistant | Resistance conc. | Is sensitive | |
|---|---|---|---|---|---|---|
| 118698 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) | |||||
| 829 | 6472 | Lincomycin | 15 µg (disc) | from Antibiotic test | ||
| 829 | 7809 | Oxacillin | 5 µg (disc) | from Antibiotic test |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 118698 | alcohol dehydrogenase | + | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | + | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68368 | arginine dihydrolase | - | 3.5.3.6 | from API 20E |
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 118698 | beta-galactosidase | + | 3.2.1.23 | |
| 68368 | beta-galactosidase | + | 3.2.1.23 | from API 20E |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 31279 | catalase | + | 1.11.1.6 | |
| 118698 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 31279 | cytochrome oxidase | + | 1.9.3.1 | |
| 68368 | cytochrome oxidase | - | 1.9.3.1 | from API 20E |
| 68382 | esterase (C 4) | - | from API zym | |
| 68382 | esterase lipase (C 8) | - | from API zym | |
| 31279 | gelatinase | + | ||
| 118698 | gelatinase | - | ||
| 68368 | gelatinase | - | from API 20E | |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | - | from API zym | |
| 118698 | lysine decarboxylase | + | 4.1.1.18 | |
| 68368 | lysine decarboxylase | + | 4.1.1.18 | from API 20E |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 118698 | ornithine decarboxylase | - | 4.1.1.17 | |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 118698 | oxidase | - | ||
| 118698 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 118698 | tryptophan deaminase | - | ||
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 118698 | urease | - | 3.5.1.5 | |
| 68368 | urease | - | 3.5.1.5 | from API 20E |
| 68382 | valine arylamidase | - | from API zym |
| Topic | Title | Authors | Journal | DOI | Year | |
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| Human microbiome derived synthetic antimicrobial peptides with activity against Gram-negative, Gram-positive, and antibiotic resistant bacteria. | Mousa WK, Shaikh AY, Ghemrawi R, Aldulaimi M, Al Ali A, Sammani N, Khair M, Helal MI, Al-Marzooq F, Oueis E. | RSC Med Chem | 10.1039/d4md00383g | 2024 | | |
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| Polyvalent Bacterial Lysate with Potential Use to Treatment and Control of Recurrent Urinary Tract Infections. | Acevedo-Monroy SE, Rocha-Ramirez LM, Martinez Gomez D, Basurto-Alcantara FJ, Medina-Contreras O, Hernandez-Chinas U, Quinones-Pena MA, Garcia-Sosa DI, Ramirez-Lezama J, Rodriguez-Garcia JA, Gonzalez-Villalobos E, Castro-Luna R, Martinez-Cristobal L, Eslava-Campos CA. | Int J Mol Sci | 10.3390/ijms25116157 | 2024 | | |
| Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides. | van Zyl EM, Coburn JM. | Int J Mol Sci | 10.3390/ijms25031462 | 2024 | | |
| Indole-3-acetic acid (IAA) protects Azospirillum brasilense from indole-induced stress. | Ganusova EE, Banerjee I, Seats T, Alexandre G. | Appl Environ Microbiol | 10.1128/aem.02384-24 | 2025 | | |
| Pathogenicity | Probiotics Modulate Host Immune Response and Interact with the Gut Microbiota: Shaping Their Composition and Mediating Antibiotic Resistance. | Mousa WK, Mousa S, Ghemrawi R, Obaid D, Sarfraz M, Chehadeh F, Husband S. | Int J Mol Sci | 10.3390/ijms241813783 | 2023 | |
| Unveiling the Hybrid Genome Structure of Escherichia coli RR1 (HB101 RecA+). | Jeong H, Sim YM, Kim HJ, Lee SJ. | Front Microbiol | 10.3389/fmicb.2017.00585 | 2017 | | |
| In silico Analyses of Core Proteins and Putative Effector and Immunity Proteins for T6SS in Enterohemorrhagic E. coli. | Vazquez-Lopez J, Navarro-Garcia F. | Front Cell Infect Microbiol | 10.3389/fcimb.2020.00195 | 2020 | | |
| Metabolism | Identification of Odor Blend Used by Caenorhabditis elegans for Pathogen Recognition. | Worthy SE, Rojas GL, Taylor CJ, Glater EE. | Chem Senses | 10.1093/chemse/bjy001 | 2018 | |
| Bacteria and Bacterial Components as Natural Bio-Nanocarriers for Drug and Gene Delivery Systems in Cancer Therapy. | Zong R, Ruan H, Liu C, Fan S, Li J. | Pharmaceutics | 10.3390/pharmaceutics15102490 | 2023 | | |
| Outer Membrane Vesicles and Soluble Factors Released by Probiotic Escherichia coli Nissle 1917 and Commensal ECOR63 Enhance Barrier Function by Regulating Expression of Tight Junction Proteins in Intestinal Epithelial Cells. | Alvarez CS, Badia J, Bosch M, Gimenez R, Baldoma L. | Front Microbiol | 10.3389/fmicb.2016.01981 | 2016 | | |
| Metabolism | Oral consumption of cranberry juice cocktail inhibits molecular-scale adhesion of clinical uropathogenic Escherichia coli. | Tao Y, Pinzon-Arango PA, Howell AB, Camesano TA. | J Med Food | 10.1089/jmf.2010.0154 | 2011 | |
| Metabolism | The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917. | Toloza L, Gimenez R, Fabrega MJ, Alvarez CS, Aguilera L, Canas MA, Martin-Venegas R, Badia J, Baldoma L. | BMC Microbiol | 10.1186/s12866-015-0591-5 | 2015 | |
| Identification of attractive odorants released by preferred bacterial food found in the natural habitats of C. elegans. | Worthy SE, Haynes L, Chambers M, Bethune D, Kan E, Chung K, Ota R, Taylor CJ, Glater EE. | PLoS One | 10.1371/journal.pone.0201158 | 2018 | | |
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How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive4434.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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