Escherichia coli K12 W3100 is a bacterium of the family Enterobacteriaceae.
Bacteria| @ref 20215 |
Last LPSN update
2026-02-09 11:18:45 |
| Domain Bacteria |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Enterobacterales |
| Family Enterobacteriaceae |
| Genus Escherichia |
| Species Escherichia coli |
| Full scientific name Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980) |
| Synonyms (1) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 2585 | NUTRIENT AGAR (DSMZ Medium 1) | Medium recipe at MediaDive  | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | |
|---|---|---|---|---|
| 2585 | positive | growth | 37 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68368 | 27613 ChEBI | amygdalin | - | fermentation | from API 20E |
| 68368 | 29016 ChEBI | arginine | - | hydrolysis | from API 20E |
| 68368 | 16947 ChEBI | citrate | - | assimilation | from API 20E |
| 68368 | 17634 ChEBI | D-glucose | + | fermentation | from API 20E |
| 68368 | 16899 ChEBI | D-mannitol | + | fermentation | from API 20E |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 68368 | 30849 ChEBI | L-arabinose | + | fermentation | from API 20E |
| 68368 | 62345 ChEBI | L-rhamnose | + | fermentation | from API 20E |
| 68368 | 25094 ChEBI | lysine | + | degradation | from API 20E |
| 68368 | 28053 ChEBI | melibiose | + | fermentation | from API 20E |
| 68368 | 17268 ChEBI | myo-inositol | - | fermentation | from API 20E |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 68368 | 30911 ChEBI | sorbitol | + | fermentation | from API 20E |
| 68368 | 17992 ChEBI | sucrose | - | fermentation | from API 20E |
| 68368 | 27897 ChEBI | tryptophan | + | energy source | from API 20E |
| 68368 | 16199 ChEBI | urea | - | hydrolysis | from API 20E |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68368 | arginine dihydrolase | - | 3.5.3.6 | from API 20E |
| 68368 | beta-galactosidase | + | 3.2.1.23 | from API 20E |
| 68368 | gelatinase | - | from API 20E | |
| 68368 | lysine decarboxylase | + | 4.1.1.18 | from API 20E |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 68368 | urease | - | 3.5.1.5 | from API 20E |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| In vitro antimicrobial activity of polyhexamethylene guanidine hydrochloride and polyhexamethylene biguanide against microorganisms causing bovine mastitis. | Ribeiro GL, Ambrosio SR, Parreira RLT, Pereira LF, Ambrosio MALV, Sabino IP, Dias FGG, Veneziani RCS. | Braz J Microbiol | 10.1007/s42770-025-01732-8 | 2025 | | |
| Probiotic lactobacilli mediate their immunoregulatory functions in intestinal cells via modulation of H3 histone acetylation. | Kumari A, Bhawal S, Kapila S, Kapila R. | J Appl Microbiol | 10.1093/jambio/lxac045 | 2023 | | |
| Potential Probiotic Lactobacillus rhamnosus (MTCC-5897) Inhibits Escherichia coli Impaired Intestinal Barrier Function by Modulating the Host Tight Junction Gene Response. | Bhat MI, Sowmya K, Kapila S, Kapila R. | Probiotics Antimicrob Proteins | 10.1007/s12602-019-09608-8 | 2020 | | |
| Antimicrobial Effect of Honey Phenolic Compounds against E. coli-An In Vitro Study. | Kassym L, Kussainova A, Semenova Y, McLoone P. | Pharmaceuticals (Basel) | 10.3390/ph17050560 | 2024 | | |
| Lactobacillus fermentum (MTCC-5898) supplementation renders prophylactic action against Escherichia coli impaired intestinal barrier function through tight junction modulation | Bhat MI, Kapila S, Kapila R. | Lebensm Wiss Technol | 10.1016/j.lwt.2020.109118 | 2020 | | |
| Probiotic lactobacilli mediated changes in global epigenetic signatures of human intestinal epithelial cells during Escherichia coli challenge | Bhat MI, Kumari A, Kapila S, Kapila R. | Ann Microbiol | 2019 | | ||
| Phylogeny | Epibiotic Bacteria Isolated from the Non-Indigenous Species Codium fragile ssp. fragile: Identification, Characterization, and Biotechnological Potential. | Cherif W, Ktari L, Hassen B, Ismail A, El Bour M. | Microorganisms | 10.3390/microorganisms12091803 | 2024 | |
| Pimarane Diterpenoids from Aerial Parts of Lycopus lucidus and Their Antimicrobial Activity. | Pandey J, Hwang BY, Lee HK, Poudel A. | Evid Based Complement Alternat Med | 10.1155/2022/5178880 | 2022 | | |
| Evaluation of Antioxidant, Xanthine Oxidase-Inhibitory, and Antibacterial Activity of Syzygium cumini Linn. Seed Extracts. | Pandey J, Jaishwal N, Jayswal M, Gupta DC, Dhakal B, Budean D, Lamichhane G, Devkota HP. | Plants (Basel) | 10.3390/plants14030316 | 2025 | | |
| Rapid Detection and Identification of Vancomycin-Sensitive Bacteria Using an Electrochemical Apta-Sensor. | Novakovic Z, Khalife M, Costache V, Camacho MJ, Cardoso S, Martins V, Gadjanski I, Radovic M, Vidic J. | ACS Omega | 10.1021/acsomega.3c08219 | 2024 | | |
| Metabolism | Myxobacteria-mediated synthesis of silver nanoparticles and their impregnation in wrapping paper used for enhancing shelf life of apples. | Bhople S, Gaikwad S, Deshmukh S, Bonde S, Gade A, Sen S, Brezinska A, Dahm H, Rai M. | IET Nanobiotechnol | 10.1049/iet-nbt.2015.0111 | 2016 | |
| Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed Extracts. | Bagale R, Acharya S, Gupta A, Chaudhary P, Chaudhary GP, Pandey J, Pandey J. | J Trop Med | 10.1155/2022/3898939 | 2022 | | |
| Study of Antibacterial Activity of Root Bark, Leaves, and Pericarp Extracts of Diploknema butyracea and Evaluation of Prospective Antioxidant Activity. | Chhetry AK, Dhakal S, Chaudhary L, Karki K, Khadka RB, Chaudhary GP, Bastola T, Poudel A, Aryal P, Pandey J. | J Trop Med | 10.1155/2022/6814901 | 2022 | | |
| Curcumin nanoparticles: physico-chemical fabrication and its in vitro efficacy against human pathogens. | Pandit RS, Gaikwad SC, Agarkar GA, Gade AK, Rai M. | 3 Biotech | 10.1007/s13205-015-0302-9 | 2015 | | |
| Green Adeptness in the Synthesis and Stabilization of Copper Nanoparticles: Catalytic, Antibacterial, Cytotoxicity, and Antioxidant Activities. | Din MI, Arshad F, Hussain Z, Mukhtar M. | Nanoscale Res Lett | 10.1186/s11671-017-2399-8 | 2017 | | |
| Storage-induced chemical changes in active components of honey de-regulate its antibacterial activity | Brudzynski K, Kim L. | Food Chem | 10.1016/j.foodchem.2010.11.151 | 2011 | | |
| Pathogenicity | In vitro antimicrobial activity of plant-derived diterpenes against bovine mastitis bacteria. | Fonseca AP, Estrela FT, Moraes TS, Carneiro LJ, Bastos JK, dos Santos RA, Ambrosio SR, Martins CH, Veneziani RC. | Molecules | 10.3390/molecules18077865 | 2013 | |
| Genetics | Use of Transposon Directed Insertion-Site Sequencing to Probe the Antibacterial Mechanism of a Model Honey on E. coli K-12. | Masoura M, Milner MT, Overton TW, Gkatzionis K, Lund PA. | Front Microbiol | 10.3389/fmicb.2021.803307 | 2021 | |
| MRJP1-containing glycoproteins isolated from honey, a novel antibacterial drug candidate with broad spectrum activity against multi-drug resistant clinical isolates. | Brudzynski K, Sjaarda C, Lannigan R. | Front Microbiol | 10.3389/fmicb.2015.00711 | 2015 | | |
| Ultrasound-Assisted Extraction and Biological Activities of Extracts of Brassica oleracea var. capitata. | Dal Pra V, Dolwitsch CB, Lima FO, Amaro de Carvalho C, Viana C, do Nascimento PC, Barcellos da Rosa M. | Food Technol Biotechnol | 10.17113/ftb.53.01.15.3533 | 2015 | | |
| Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice. | Sharma R, Kapila R, Dass G, Kapila S. | Age (Dordr) | 10.1007/s11357-014-9686-4 | 2014 | | |
| Effect of hydrogen peroxide on antibacterial activities of Canadian honeys. | Brudzynski K. | Can J Microbiol | 10.1139/w06-086 | 2006 | | |
| Efficacy and safety of Melaleuca alternifolia (tea tree) oil for human health-A systematic review of randomized controlled trials. | Kairey L, Agnew T, Bowles EJ, Barkla BJ, Wardle J, Lauche R. | Front Pharmacol | 10.3389/fphar.2023.1116077 | 2023 | | |
| Pathogenicity | Antibacterial compounds of Canadian honeys target bacterial cell wall inducing phenotype changes, growth inhibition and cell lysis that resemble action of beta-lactam antibiotics. | Brudzynski K, Sjaarda C. | PLoS One | 10.1371/journal.pone.0106967 | 2014 | |
| Pathogenicity | Honey glycoproteins containing antimicrobial peptides, Jelleins of the Major Royal Jelly Protein 1, are responsible for the cell wall lytic and bactericidal activities of honey. | Brudzynski K, Sjaarda C. | PLoS One | 10.1371/journal.pone.0120238 | 2015 | |
| Metabolism | Molybdate treatment and sulfate starvation decrease ATP and DNA levels in Ferroplasma acidarmanu. | Baumler DJ, Hung KF, Jeong KC, Kaspar CW. | Archaea | 10.1155/2008/762967 | 2008 | |
| Pathogenicity | Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines. | Abrao F, de Araujo Costa LD, Alves JM, Senedese JM, de Castro PT, Ambrosio SR, Veneziani RC, Bastos JK, Tavares DC, Martins CH. | BMC Complement Altern Med | 10.1186/s12906-015-0961-4 | 2015 | |
| Honey: a potential therapeutic agent for managing diabetic wounds. | Alam F, Islam MA, Gan SH, Khalil MI. | Evid Based Complement Alternat Med | 10.1155/2014/169130 | 2014 | | |
| Self-Setting Calcium Phosphate Cements with Tunable Antibiotic Release Rates for Advanced Antimicrobial Applications. | Ghosh S, Wu V, Pernal S, Uskokovic V. | ACS Appl Mater Interfaces | 10.1021/acsami.6b01160 | 2016 | | |
| Metabolism | Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production. | Brudzynski K, Miotto D, Kim L, Sjaarda C, Maldonado-Alvarez L, Fuks H. | Sci Rep | 10.1038/s41598-017-08072-0 | 2017 | |
| Mechanism of Honey Bacteriostatic Action Against MRSA and VRE Involves Hydroxyl Radicals Generated from Honey's Hydrogen Peroxide. | Brudzynski K, Lannigan R. | Front Microbiol | 10.3389/fmicb.2012.00036 | 2012 | | |
| Pathogenicity | A Review on Flavonoid Apigenin: Dietary Intake, ADME, Antimicrobial Effects, and Interactions with Human Gut Microbiota. | Wang M, Firrman J, Liu L, Yam K. | Biomed Res Int | 10.1155/2019/7010467 | 2019 | |
| Powerful bacterial killing by buckwheat honeys is concentration-dependent, involves complete DNA degradation and requires hydrogen peroxide. | Brudzynski K, Abubaker K, Wang T. | Front Microbiol | 10.3389/fmicb.2012.00242 | 2012 | | |
| SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles. | Fandl JP, Cabelli R, Oliver D, Tai PC. | Proc Natl Acad Sci U S A | 10.1073/pnas.85.23.8953 | 1988 | | |
| Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. | Brudzynski K, Abubaker K, St-Martin L, Castle A. | Front Microbiol | 10.3389/fmicb.2011.00213 | 2011 | | |
| Stress | Evaluation of the heat inactivation of Escherichia coli and Lactobacillus plantarum by differential scanning calorimetry. | Lee J, Kaletunc G. | Appl Environ Microbiol | 10.1128/aem.68.11.5379-5386.2002 | 2002 | |
| Comparative ORESTES-sampling of transcriptomes of immune-challenged Biomphalaria glabrata snails. | Hanelt B, Lun CM, Adema CM. | J Invertebr Pathol | 10.1016/j.jip.2008.06.002 | 2008 | | |
| Metabolism | Single transporter for sulfate, selenate, and selenite in Escherichia coli K-12. | Lindblow-Kull C, Kull FJ, Shrift A. | J Bacteriol | 10.1128/jb.163.3.1267-1269.1985 | 1985 | |
| Metabolism | Acetate metabolism by Escherichia coli in high-cell-density fermentation. | Kleman GL, Strohl WR. | Appl Environ Microbiol | 10.1128/aem.60.11.3952-3958.1994 | 1994 | |
| Accumulation and elimination of Escherichia coli and Salmonella typhimurium by hard clams in an in vitro system. | Timoney JF, Abston A. | Appl Environ Microbiol | 10.1128/aem.47.5.986-988.1984 | 1984 | | |
| Metabolism | Requirement of heat-labile cytoplasmic protein factors for posttranslational translocation of OmpA protein precursors into Escherichia coli membrane vesicles. | Weng QP, Chen LL, Tai PC. | J Bacteriol | 10.1128/jb.170.1.126-131.1988 | 1988 | |
| Metabolism | Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli. | Alterthum F, Ingram LO. | Appl Environ Microbiol | 10.1128/aem.55.8.1943-1948.1989 | 1989 | |
| Characterization of SmSu plasmids by restriction endonuclease cleavage and compatibility testing. | Grinter NJ, Barth PT. | J Bacteriol | 10.1128/jb.128.1.394-400.1976 | 1976 | | |
| Metabolism | Identification of the forms of vitamin B 6 present in the culture media of "vitamin B 6 control" mutants. | Dempsey WB, Arcement LJ. | J Bacteriol | 10.1128/jb.107.2.580-582.1971 | 1971 | |
| Pathogenicity | R-factors of Escherichia coli from dressed beef and humans. | Babcock GF, Berryhill DL, Marsh DH. | Appl Microbiol | 10.1128/am.25.1.21-23.1973 | 1973 | |
| Metabolism | 3-hydroxypyruvate substitutes for pyridoxine in serC mutants of Escherichia coli K-12. | Shimizu S, Dempsey WB. | J Bacteriol | 10.1128/jb.134.3.944-949.1978 | 1978 | |
| Multiple chromosomes of Azotobacter vinelandii. | Nagpal P, Jafri S, Reddy MA, Das HK. | J Bacteriol | 10.1128/jb.171.6.3133-3138.1989 | 1989 | | |
| Enzymology | Antimicrobial Polymers in the Nano-World. | Alvarez-Paino M, Munoz-Bonilla A, Fernandez-Garcia M. | Nanomaterials (Basel) | 10.3390/nano7020048 | 2017 | |
| Metabolism | Oxidation of protoporphyrinogen in the obligate anaerobe Desulfovibrio gigas. | Klemm DJ, Barton LL. | J Bacteriol | 10.1128/jb.164.1.316-320.1985 | 1985 | |
| Honey for wound healing, ulcers, and burns; data supporting its use in clinical practice. | Al-Waili N, Salom K, Al-Ghamdi AA. | ScientificWorldJournal | 10.1100/tsw.2011.78 | 2011 | | |
| Enzymology | Vivo clearance of enteric bacteria from the hemolymph of the hard clam and the American oyster. | Hartland BJ, Timoney JF. | Appl Environ Microbiol | 10.1128/aem.37.3.517-520.1979 | 1979 | |
| Effect of entry exclusion on mating aggregates and transconjugants. | Eckerson HW, Reynard AM. | J Bacteriol | 10.1128/jb.129.1.131-137.1977 | 1977 | | |
| Metabolism | Uptake of 3H-Tetracycline by resistant and sensitive Escherichia coli. | Reynard AM, Nellis LF, Beck ME. | Appl Microbiol | 10.1128/am.21.1.71-75.1971 | 1971 | |
| Pathogenicity | Transferable drug resistance in Pseudomonas aeruginosa. | Bryan LE, Van Den Elzen HM, Tseng JT. | Antimicrob Agents Chemother | 10.1128/aac.1.1.22 | 1972 | |
| The serC-aro A operon of Escherichia coli. A mixed function operon encoding enzymes from two different amino acid biosynthetic pathways. | Duncan K, Coggins JR. | Biochem J | 10.1042/bj2340049 | 1986 | | |
| Metabolism | Transport systems for alanine, serine, and glycine in Escherichia coli K-12. | Robbins JC, Oxender DL. | J Bacteriol | 10.1128/jb.116.1.12-18.1973 | 1973 | |
| Metabolism | Rapid enumeration of Fecal Coliforms in water by a colorimetric beta-galactosidase assay. | Warren LS, Benoit RE, Jessee JA. | Appl Environ Microbiol | 10.1128/aem.35.1.136-141.1978 | 1978 | |
| Metabolism | Transport of L-4-azaleucine in Escherichia coli. | Harrison LI, Christensen HN, Handlogten ME, Oxender DL, Quay SC. | J Bacteriol | 10.1128/jb.122.3.957-965.1975 | 1975 | |
| Pathogenicity | Mode of action illudin S. | Walser J, Heinstein PF. | Antimicrob Agents Chemother | 10.1128/aac.3.3.357 | 1973 | |
| Metabolism | Role of transport systems in amino acid metabolism: leucine toxicity and the branched-chain amino acid transport systems. | Quay SC, Dick TE, Oxender DL. | J Bacteriol | 10.1128/jb.129.3.1257-1265.1977 | 1977 | |
| Pathogenicity | Characteristics of R931 and other Pseudomonas aeruginosa R factors. | Bryan LE, Semaka SD, Van den Elzen HM, Kinnear JE, Whitehouse RL. | Antimicrob Agents Chemother | 10.1128/aac.3.5.625 | 1973 | |
| Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides. | Barth PT, Grinter NJ. | J Bacteriol | 10.1128/jb.120.2.618-630.1974 | 1974 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive4620.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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