Strain identifier

BacDive ID: 4639

Type strain: No

Species: Escherichia coli

Strain Designation: K12 DH5alpha, DH5alpha

Strain history: <- CCRC <- Gibco BRL Life Technologies

NCBI tax ID(s): 562 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2879

BacDive-ID: 4639

DSM-Number: 6897

keywords: genome sequence, Bacteria, mesophilic

description: Escherichia coli K12 DH5alpha is a mesophilic bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 2879
  • history: <- CCRC <- Gibco BRL Life Technologies

doi: 10.13145/bacdive4639.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 2879

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: K12 DH5alpha, DH5alpha

type strain: no

Culture and growth conditions

culture medium

  • @ref: 2879
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/381
  • composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 2879
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836817997dinitrogenyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368arginine dihydrolase-3.5.3.6

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAN2OX
2879--------+--++-++-+-++
2879+-+-----+--+--++---++
2879--------+--++-++-+-++-
2879--------+--++-++-+-++-
2879--------+--++-++-+-++-

Safety information

risk assessment

  • @ref: 2879
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Escherichia coli DH5alphaGCA_002899475completencbi562
66792Escherichia coli DH5alphaGCA_002848225completencbi562
66792Escherichia coli DH5alphaGCA_009648495completencbi562
66792Escherichia coli DH5alphaGCA_006352225contigncbi562

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno58.085no
gram-positiveno98.257no
anaerobicno94.593no
aerobicyes86.238no
halophileno90.91no
spore-formingno93.982no
glucose-utilyes94.233no
thermophileno98.877yes
motileyes88.951no
glucose-fermentyes93.081yes

External links

@ref: 2879

culture collection no.: DSM 6897, CCRC 51731

straininfo link

  • @ref: 74134
  • straininfo: 19187

Reference

@idauthorscataloguedoi/urltitle
2879Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6897)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6897
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
74134Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID19187.1StrainInfo: A central database for resolving microbial strain identifiers