Staphylococcus aureus (DSM 29134, EDCC-5443, EDCC 5443)
Staphylococcus aureus EDCC-5443 is a human pathogen that has multiple antibiotic resistances and was isolated from human patient with implant problems, nose.
antibiotic resistance human pathogen BacteriaName and taxonomic classification
| @ref 20215 |
Last LPSN update
2026-05-29 08:30:39 |
| Domain Bacteria |
| Phylum Bacillota |
| Class Bacilli |
| Order Caryophanales |
| Family Staphylococcaceae |
| Genus Staphylococcus |
| Species Staphylococcus aureus |
| Full scientific name Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980) |
| Synonyms (2) |
Culture and growth conditions
Culture medium
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 20836 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | Medium recipe at MediaDive  | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water | ||
| 20836 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | Medium recipe at MediaDive | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
Culture temp
| @ref | Growth | Type | Temperature (°C) | |
|---|---|---|---|---|
| 20836 | positive | growth | 37 |
Physiology and metabolism
Antibiotic susceptibility testing
| Test 1 | Test 2 | |
|---|---|---|
| @ref | 20836 | 20836 |
| Medium | Mueller-Hinton Agar | Mueller-Hinton Agar |
| Manual annotation | 1 | 1 |
| Inhibition zone diameter in mm | Inhibition zone diameter in mm | |
| Amikacin 30µg (disc) | 26 | 22 |
| Ampicillin 10µg (disc) | 14-16 | 14 |
| Aztreonam 30µg (disc) | 0 | 0 |
| Bacitracin 10Unit | 18-20 | 20 |
| Cefalotin 30µg (disc) | 24 | 22 |
| Cefazolin 30µg (disc) | 18 | 16-18 |
| Cefotaxime 30µg (disc) | 14-16 | 12-14 |
| Ceftriaxone 30µg (disc) | 12-14 | 12-14 |
| Chloramphenicol 30µg (disc) | 26 | 26-28 |
| Clindamycin 10µg (disc) | 0 | 0 |
| Colistin 10µg (disc) | 0 | 0 |
| Doxycycline 30µg (disc) | 30-32 | 34 |
| Erythromycin 15µg (disc) | 0 | 0 |
| Fosfomycin 50µg (disc) | 30-32 | 32-34 |
| Gentamicin 10µg (disc) | 24 | 24 |
| Imipenem 10µg (disc) | 36 | 34 |
| Kanamycin 30µg (disc) | 26 | 24 |
| Lincomycin 15µg (disc) | 0 | 0 |
| Linezolid 10µg (disc) | 32 | 32 |
| Mezlocillin 30µg (disc) | 16 | 14-16 |
| Moxifloxacin 5µg (disc) | 16 | 12-14 |
| Neomycin 30µg (disc) | 24 | 22 |
| Nitrofurantoin 100µg (disc) | 20 | 22 |
| Norfloxacin 10µg (disc) | 0 | 0 |
| Nystatin 100Unit | 0 | 0 |
| Ofloxacin 5µg (disc) | 0 | 0 |
| Oxacillin 5µg (disc) | 10 | 8 |
| Penicillin G 6µg (disc) | 12 | 12 |
| Pipemidic acid 20µg (disc) | 0 | 0 |
| Piperacillin/Tazobactam 40µg (disc) | 20 | 20 |
| Polymyxin B 300Unit | 10-12 | 6-8 |
| Quinupristin/Dalfopristin 15µg (disc) | 26 | 28 |
| Teicoplanin 30µg (disc) | 18 | 18 |
| Tetracycline 30µg (disc) | 32 | 34 |
| Ticarcillin 75µg (disc) | 20 | 22 |
| Vancomycin 30µg (disc) | 18-20 | 20 |
Metabolite utilization
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68375 | 22599 ChEBI | arabinose | - | fermentation | from API ID32STA |
| 68375 | 15824 ChEBI | D-fructose | + | fermentation | from API ID32STA |
| 68375 | 17634 ChEBI | D-glucose | + | fermentation | from API ID32STA |
| 68375 | 16899 ChEBI | D-mannitol | + | fermentation | from API ID32STA |
| 68375 | 16024 ChEBI | D-mannose | + | fermentation | from API ID32STA |
| 68375 | 16988 ChEBI | D-ribose | - | fermentation | from API ID32STA |
| 68375 | 17716 ChEBI | lactose | + | fermentation | from API ID32STA |
| 68375 | 17306 ChEBI | maltose | + | fermentation | from API ID32STA |
| 68375 | 59640 ChEBI | N-acetylglucosamine | + | fermentation | from API ID32STA |
| 68375 | 17632 ChEBI | nitrate | + | reduction | from API ID32STA |
| 68375 | 18257 ChEBI | ornithine | - | degradation | from API ID32STA |
| 68375 | 16634 ChEBI | raffinose | - | fermentation | from API ID32STA |
| 68375 | 17992 ChEBI | sucrose | + | fermentation | from API ID32STA |
| 68375 | 27082 ChEBI | trehalose | + | fermentation | from API ID32STA |
| 68375 | 32528 ChEBI | turanose | + | fermentation | from API ID32STA |
| 68375 | 16199 ChEBI | urea | - | hydrolysis | from API ID32STA |
Antibiotic resistance
| @ref | ChEBI | Group ID | Metabolite | Is resistant | Resistance conc. | |
|---|---|---|---|---|---|---|
| 20836 | 161680 | Aztreonam | 30 µg (disc) | from Antibiotic test | ||
| 20836 | 3745 | Clindamycin | 10 µg (disc) | from Antibiotic test | ||
| 20836 | 37943 | Colistin | 10 µg (disc) | from Antibiotic test | ||
| 20836 | 48923 | Erythromycin | 15 µg (disc) | from Antibiotic test | ||
| 20836 | 6472 | Lincomycin | 15 µg (disc) | from Antibiotic test | ||
| 20836 | 100246 | Norfloxacin | 10 µg (disc) | from Antibiotic test | ||
| 68375 | 28368 | 0 | novobiocin | 1.8 µg | from API ID32STA | |
| 20836 | 7660 | Nystatin | 100 Unit | from Antibiotic test | ||
| 20836 | 7731 | Ofloxacin | 5 µg (disc) | from Antibiotic test | ||
| 20836 | 75250 | Pipemidic acid | 20 µg (disc) | from Antibiotic test |
Metabolite production
| @ref | Chebi-ID | Metabolite | Production | |
|---|---|---|---|---|
| 68375 | 15688 ChEBI | acetoin | from API ID32STA |
Metabolite tests
| @ref | Chebi-ID | Metabolite | Voges-proskauer-test | |
|---|---|---|---|---|
| 68375 | 15688 ChEBI | acetoin | + | from API ID32STA |
Enzymes
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68375 | alkaline phosphatase | + | 3.1.3.1 | from API ID32STA |
| 68375 | beta-glucuronidase | - | 3.2.1.31 | from API ID32STA |
| 20836 | catalase | + | 1.11.1.6 | |
| 20836 | cytochrome-c oxidase | - | 1.9.3.1 | |
| 68375 | L-arginine arylamidase | - | from API ID32STA | |
| 68375 | ornithine decarboxylase | - | 4.1.1.17 | from API ID32STA |
| 68375 | urease | - | 3.5.1.5 | from API ID32STA |
API ID32STA
| @ref | URE | ADH (Arg) | ODC | ESC | GLU | FRU | MNE | MAL | LAC | TRE | MAN | RAF | RIB | CEL | Reduction of nitrateNIT | Acetoin production (Voges Proskauer test)VP | beta GAL | L-arginine arylamidaseArgA | PAL | Pyrrolidonyl arylamidasePyrA | NOVO | SAC | NAG | TUR | ARA | beta GUR | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 20836 | - | - | - | + | + | + | + | + | + | + | + | - | - | - | + | + | - | - | + | - | + | + | + | + | - | - | |
| 20836 | - | + | - | +/- | + | + | + | + | + | + | + | - | - | +/- | + | + | + | - | + | +/- | + | + | + | + | - | - |
Literature
Literature 
| Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|
| Comparison between Some Phenotypic and Genotypic Methods for Assessment of Antimicrobial Resistance Trend of Bovine Mastitis Staphylococcus aureus Isolates from Bulgaria. | Rusenova N, Vasilev N, Rusenov A, Milanova A, Sirakov I. | Vet Sci | 10.3390/vetsci9080401 | 2022 | |
| High Adherence of Oral Streptococcus to Polylactic Acid Might Explain Implant Infections Associated with PLA Mesh Implantation. | Sarfraz S, Tamminen AM, Leikola J, Salmi S, Kaakinen M, Sorsa T, Suojanen J, Reunanen J. | Int J Mol Sci | 10.3390/ijms24119504 | 2023 | |
| Comparison of Titanium and PEEK Medical Plastic Implant Materials for Their Bacterial Biofilm Formation Properties. | Sarfraz S, Mantynen PH, Laurila M, Rossi S, Leikola J, Kaakinen M, Suojanen J, Reunanen J. | Polymers (Basel) | 10.3390/polym14183862 | 2022 | |
| Effect of Surface Tooling Techniques of Medical Titanium Implants on Bacterial Biofilm Formation In Vitro. | Sarfraz S, Mantynen PH, Laurila M, Suojanen J, Saarnio J, Rossi S, Horelli J, Kaakinen M, Leikola J, Reunanen J. | Materials (Basel) | 10.3390/ma15093228 | 2022 | |
How to cite
If you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive24592.20260601.11
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain dataIsabel Schober, Julia Koblitz, Joaquim Sardà Carbasse, Christian Ebeling, Marvin Leon Schmidt, Adam Podstawka, Rohit Gupta, Vinodh Ilangovan, Javad Chamanara, Jörg Overmann, Lorenz Christian Reimer
Nucleic Acids Research; database issue 2025.
License