Name: Pseudomonas putida CIP 59.25
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 135611

Type strain:

Species: Pseudomonas putida

Culture col. no.: CIP 59.25, ATCC 12842, NCIMB 8865, JCM 13060, CECT 385

Strain history: CIP <- 1959, NCIB, Pseudomonas fluorescens <- J. Peel, Sheffield Univ., UK

NCBI tax ID(s): 303 (species)

SI-ID 35675

ATCC 12842, NCIB 8865, NCIMB 8865, CIP 59.25, CECT 385, NCIM 2872

Other strains from Pseudomonas putida

P. putida 90, A 3.12, DSM 291, ATCC 12633, ... (type strain)
P. putida 1593, DSM 295
P. putida C-1, DSM 437, ATCC 23974, DSM 50253
P. putida 77, DSM 1693, ATCC 8209, CCM 380, ...
P. putida 12/5, DSM 1868
P. putida PaW 340, DSM 2112
P. putida DSM 3226
P. putida mt-2 PaW1, pWWO, DSM 3931, ATCC ...
P. putida MT20, pWW20, DSM 3932, NCIB 12186
P. putida MT15, pWW15, DSM 3936, NCIB 12184
P. putida PaW611, pWW53-4, DSM 3937
P. putida PaW701, pWW60-1, DSM 3938, NCIB ...
P. putida KF439, pKF439, DSM 4011
P. putida PaW736, pWW60-22, DSM 4302, NCIB ...
P. putida MT53, pWW53, DSM 4303, NCIB 12187
P. putida CAM, PpG1, DSM 4475
P. putida NAH, PpG7, DSM 4476
P. putida NIC, PpG25, DSM 4477
P. putida PpG2100, SAL, DSM 4478
P. putida PaW 130, DSM 5169
P. putida PaW 95, DSM 5170
P. putida CLB250, DSM 5232
P. putida DSM 84, IMG 1513
P. putida mt-2 KT2440, DSM 6125, ATCC 47054, ...
P. putida S-832, DSM 6883
P. putida S-313, DSM 6884
P. putida BG1, DSM 6413
P. putida DSM 6521, ATCC 11172, NCIB 8251, ...
P. putida pUTK9, DSM 6701, RB 1351
P. putida US2, DSM 6755
P. putida F1, PpF1, DSM 6899, ATCC 700007, ...
P. putida PS, DSM 7189
P. putida Strain G, DSM 7286, BKM BN-1123, ...
P. putida Strain A, DSM 7314, NCIMB 11286, ...
P. putida MM1, DSM 9278
P. putida RW20-3b, DSM 11738
P. putida GS1, DSM 12264
P. putida 2-2L, DSM 12585
P. putida PNP1, DSM 12448
P. putida U, DSM 12735, ATCC 17514, NCIMB ...
P. putida DSM 13624, IMI 358533
P. putida TM1, DSM 16845
P. putida 50-3-3, IBPM B-20, DSM 46608, CCEB ...
P. putida Berlin 33/2, DSM 50026, CIP 103281
P. putida cl-B, DSM 50198, ATCC 17453, ICPB ...
P. putida 53-2, DSM 50208, ATCC 17485, ICPB ...
P. putida DSM 50257, ATCC 6876, DSM 60118, ...
P. putida MT-I1, DSM 21680
P. putida WB, DSM 23849
P. putida ST033293(HKI),
P. putida 160, DSM 50194, ATCC 17529, ICPB ...
P. putida MS of E.E. Snell, DSM 50204, ATCC ...
P. putida S16, DSM 28022
P. putida B6-2, DSM 28064
P. putida SQ1, DSM 100120
P. putida Z2, DSM 16527
P. putida TEC1, DSM 26234
P. putida TEC1 407.1-delta 2, DSM 26250
P. putida TEC1 407.3-delta2, DSM 26251
P. putida TEC1 407-delta1, DSM 26252
P. putida S13.1.2, DSM 102034
P. putida TEC1 91-delta 1, DSM 26253
P. putida AmMs 106, CIP 104063, ATCC 49128
P. putida CIP 55.5, ATCC 795, IAM 1505, NCDO ...
P. putida A3/9, CIP 59.26, NCIMB 8248
P. putida CIP 108916, LMG 1246, ATCC 17430, ...
P. putida CIP 52.195
P. putida CIP 52.196
P. putida CIP 52.193
P. putida CIP 52.197
P. putida CIP 52.198
P. putida CIP 52.194
P. putida CIP 52.190
P. putida 25300, 642-85, CIP 101794
P. putida 340-86, CIP 102341
P. putida 776.85, CIP 101936
P. putida MD 7.3, CIP 59.19, NCIMB 8729
P. putida CIP 52.189
P. putida CCUG 264
P. putida CCUG 553, LMG 2189
P. putida CCUG 2471
P. putida CCUG 2479
P. putida CCUG 2525, NRRL B-2022
P. putida CCUG 15619
P. putida CCUG 18875
P. putida CCUG 27052
P. putida CCUG 28920
P. putida CCUG 29643
P. putida CCUG 30612
P. putida CCUG 31110
P. putida CCUG 32007
P. putida CCUG 33113
P. putida CCUG 33582
P. putida CCUG 33721
P. putida CCUG 34242
P. putida CCUG 35570
P. putida CCUG 37253
P. putida CCUG 42126
P. putida CCUG 49576
P. putida CCUG 49716
P. putida CCUG 50315
P. putida CCUG 50317
P. putida CCUG 54971
P. putida CCUG 57833
P. putida CCUG 60837
P. putida CCUG 61918
P. putida JCM 13062, ATCC 11250, CCM 3656, ...
P. putida JCM 14351, ATCC 598, NRRL B-251
P. putida JCM 18798
P. putida JCM 20004, IAM 1002
P. putida JCM 20005, IAM 1003
P. putida JCM 20028, IAM 1049
P. putida JCM 20029, IAM 1050
P. putida JCM 20048, IAM 1094
P. putida JCM 20066, IAM 1128
P. putida JCM 20089, IAM 1177
P. putida JCM 20102, IAM 1200
P. putida JCM 20111, IAM 1218
P. putida JCM 20112, ATCC 17464, IAM 1219
P. putida JCM 20120, IAM 1235
P. putida JCM 20129, IAM 1250
P. putida JCM 20188, ATCC 4359, IAM 1506
P. putida JCM 20228, IAM 1629
P. putida JCM 9045
P. putida JCM 9800
P. putida JCM 9802

Section

Pseudomonas putida CIP 59.25 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from Soil.

Gram-negative
motile
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (LPSN)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on morphological and physiological properties Morphology

[Ref.: #34785]	Gram stain	negative

[Ref.: #34785]	Cell shape	rod-shaped

[Ref.: #34785]	Motility	yes

[Ref.: #34785]	Pigment production	no
[Ref.: #34785]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #34785]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #34785]

Culture medium growth

[Ref.: #34785]

Culture medium composition

expand / minimize

[Ref.: #34785]

Culture medium

CIP Medium 72

[Ref.: #34785]

Culture medium growth

[Ref.: #34785]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #34785]

Culture medium

CIP Medium 3

[Ref.: #34785]

Culture medium growth

[Ref.: #34785]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #34785]		growth	30 °C
[Ref.: #34785]		growth	5-37 °C
[Ref.: #34785]	no	growth	41 °C
[Ref.: #67770]		growth	30 °C

[Ref.: #34785]	Temperature range	mesophilic
[Ref.: #34785]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #34785]

Oxygen tolerance

obligate aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #34785]		NaCl		growth	0-8	%
[Ref.: #34785]		NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #34785]	citrate ChEBI	+	carbon source
[Ref.: #34785]	esculin ChEBI	-	hydrolysis
[Ref.: #34785]	nitrate ChEBI	-	reduction
[Ref.: #34785]	nitrate ChEBI	-	respiration
[Ref.: #34785]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #34785]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #34785]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #34785]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #34785]	amylase	-
[Ref.: #34785]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #34785]	caseinase	-	3.4.21.50
[Ref.: #34785]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #34785]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #34785]	gelatinase	-
[Ref.: #34785]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #34785]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #34785]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #34785]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #34785]	oxidase	+
[Ref.: #34785]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #34785]	tryptophan deaminase	-
[Ref.: #34785]	tween esterase	+
[Ref.: #34785]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #34785]	3148	not determinedn.d.	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	-	-	+	+	+	+	+	-	-	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #34785]	12956	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Soil

[Ref.: #34785]	Sample type/isolated from	Environment, Soil
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence LC507957 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	543
Soil Samples	368
Animal Samples	799
Plant Samples	423
Total Samples	2133

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #34785]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudomonas fluorescens JCM 13060 gene for 16S rRNA, partial sequence	LC507957	1459	ENA	294 tax ID

Availability in culture collections External links

[Ref.: #34785]

Culture collection no.

CIP 59.25, ATCC 12842, NCIMB 8865, JCM 13060, CECT 385

[Ref.: #92935]

SI-ID 35675

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#34785 Collection of Institut Pasteur ; Curators of the CIP; CIP 59.25
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#92935

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID35675.1 )

* These data were automatically processed and therefore are not curated

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