Name: Pseudomonas putida U
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12914

Type strain:

Species: Pseudomonas putida

Strain Designation: U

Culture col. no.: DSM 12735, ATCC 17514, NCIMB 10015, CCUG 2869, DSM 548

Strain history: <- NCIMB <- E. M. Chamberlain; U

NCBI tax ID(s): 303 (species)

Other strains from Pseudomonas putida

Section

Pseudomonas putida U is a mesophilic human pathogen that was isolated from mud of a stream.

human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (PNU)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4796]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #4796]

Culture medium growth

[Ref.: #4796]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4796]

Culture medium composition

expand / minimize

[Ref.: #4796]

Culture medium

MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)

[Ref.: #4796]

Culture medium growth

[Ref.: #4796]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4796]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #4796]			growth	28 °C

[Ref.: #4796]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44597]

Fatty acid	Percentage	ECL
C16:0	25.8	16
C16:1 ω7c	25.3	15.819
C18:1 ω7c /12t/9t	12.4	17.824
C17:0 CYCLO	12.1	16.888
C12:0 2OH	9.2	13.178
C10:0 3OH	5.7	11.423
C12:0 3OH	4.3	13.455
C12:0	1.7	12
C14:0	1	14
C18:0	0.8	18
C15:0	0.7	15
C18:2 ω6,9c/C18:0 ANTE	0.6	17.724
Unidentified	0.5	18.564

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #4796]	20253	-	-	-	+	-	-	-	-	+	-	-	+	-	-	+	+	-	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4796]	Sample type/isolated from	mud of a stream
[Ref.: #4796]	Geographic location (country and/or sea, region)	Illinois, Urbana
[Ref.: #4796]	Country	USA
[Ref.: #4796]	Country ISO 3 Code	USA
[Ref.: #4796]	Continent	North America

[Ref.: #44597]	Sample type/isolated from	Mud of a stream
[Ref.: #44597]	Geographic location (country and/or sea, region)	Illinois,Urbana
[Ref.: #44597]	Country	USA
[Ref.: #44597]	Country ISO 3 Code	USA
[Ref.: #44597]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Aquatic	#River (Creek)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF094741 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	543
Soil Samples	368
Animal Samples	799
Plant Samples	423
Total Samples	2133

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4796]	Pathogenicity (human)	yes
[Ref.: #4796]	Pathogenicity (animal)	yes
[Ref.: #4796]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Pseudomonas putida strain ATCC 17514 16S ribosomal RNA gene, partial sequence	AF094741	1477	ENA	303 tax ID	*

Availability in culture collections External links

[Ref.: #4796]

Culture collection no.

DSM 12735, ATCC 17514, NCIMB 10015, CCUG 2869, DSM 548

[Ref.: #20218]

Associated Passport(s) in StrainInfo

163327, 99220, 163328

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Biodegradation of mixed wastes in continuously operated cyclic reactors.	Wang KW, Tsangaris DM, Baltzis BC, Lewandowski GA	Appl Biochem Biotechnol	10.1007/BF02941760	1996	*
Metabolism	Phenol biodegradation by Pseudomonas putida DSM 548 in a batch reactor.	Monteiro1 AA, Boaventura RA, Rodrigues AE	Biochem Eng J	10.1016/s1369-703x(00)00072-3	2000	*
Metabolism	Three-dimensional distribution of GFP-labeled Pseudomonas putida during biofilm formation on solid PAHs assessed by confocal laser scanning microscopy.	Rodrigues AC, Wuertz S, Brito AG, Melo LF	Water Sci Technol		2003	*
Enzymology	Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects.	Rodrigues AC, Wuertz S, Brito AG, Melo LF	Biotechnol Bioeng	10.1002/bit.20377	2005	*
Enzymology	Kinetics of phenol biodegradation in the presence of glucose.	Wang KW, Baltzis BC, Lewandowski GA	Biotechnol Bioeng	10.1002/(SICI)1097-0290(19960705)51:1<87::AID-BIT10>3.0.CO;2-1	1996	*
Metabolism	A standardized pre-treatment method of biofilm flocs for fluorescence microscopic characterization.	Nosyk O, ter Haseborg E, Metzger U, Frimmel FH	J Microbiol Methods	10.1016/j.mimet.2008.07.024	2008	*
	Competition between two microbial populations in a sequencing fed-batch reactor: theory, experimental verification, and implications for waste treatment applications.	Dikshitulu S, Baltzis BC, Lewandowski GA, Pavlou S	Biotechnol Bioeng	10.1002/bit.260420513	1993	*
	Efficacy of olive leaf extract (Olea europaea L. cv Gentile di Larino) in marinated anchovies (Engraulis encrasicolus, L.) process.	Testa B, Lombardi SJ, Macciola E, Succi M, Tremonte P, Iorizzo M	Heliyon	10.1016/j.heliyon.2019.e01727	2019	*

References

#4796

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12735

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#44597 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 2869

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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