Name: Pseudomonas putida mt-2 PaW1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12880

Type strain:

Species: Pseudomonas putida

Strain Designation: mt-2 PaW1, pWWO

Culture col. no.: DSM 3931, ATCC 33015, NCIB 12182, CCUG 2480, ATCC 23973, JCM 6156, BCRC 10460

Strain history: ATCC 33015 <-- P. A. Williams mt-2 <-- G. D. Hegeman.

NCBI tax ID(s): 303 (species)

SI-ID 92520

LMG 16335, ATCC 23973, CCRC 13843, CCUG 2480, NCIB 10432, NCIMB 10432, KCTC 1643, BCRC 13843

Other strains from Pseudomonas putida

Section

Pseudomonas putida mt-2 PaW1 is a mesophilic bacterium that was isolated from Soil using benzoate as a major carbon source.

mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (LPSN)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on culture and growth conditions Culture and growth conditions

	Temperatures	Kind of temperature		Temperature
[Ref.: #44546]			growth	26 °C
[Ref.: #67770]			growth	30 °C

[Ref.: #44546]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #1518]	24331	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+
[Ref.: #1518]	24330	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+
[Ref.: #1518]	24329	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+
[Ref.: #44546]	7455	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #44546]	3780	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44546]	Sample type/isolated from	Soil using benzoate as a major carbon source
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB598738 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	543
Soil Samples	368
Animal Samples	799
Plant Samples	423
Total Samples	2133

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1518]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudomonas putida gene for 16S rRNA, partial sequence, strain: JCM 6156	AB598738	1459	ENA	303 tax ID
[Ref.: #20218]	Pseudomonas putida (strain JCM 6156 (ATCC 33015)) gene for 16S ribosomal RNA, partial sequence	D37924	1329	ENA	303 tax ID	*

Availability in culture collections External links

[Ref.: #1518]

Culture collection no.

DSM 3931, ATCC 33015, NCIB 12182, CCUG 2480, ATCC 23973, JCM 6156, BCRC 10460

[Ref.: #82092]	SI-ID 92520	*
[Ref.: #82093]	SI-ID 112627	*

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	High-yield and plasmid-free biocatalytic production of 5-methylpyrazine-2-carboxylic acid by combinatorial genetic elements engineering and genome engineering of Escherichia coli.	Gu L, Yuan H, Lv X, Li G, Cong R, Li J, Du G, Liu L	Enzyme Microb Technol	10.1016/j.enzmictec.2019.109488	2019	*
Enzymology	Iron(III) coordination properties of a pyoverdin siderophore produced by Pseudomonas putida ATCC 33015.	Boukhalfa H, Reilly SD, Michalczyk R, Iyer S, Neu MP	Inorg Chem	10.1021/ic060196p	2006	*
Metabolism	Analysis of a simple biodegradation process for the removal of volatile organic chemicals from wastewater based on a gas stripping principle.	Dahlan MH, Xing XH, Yoshikawa Y, Matsumoto K	J Biosci Bioeng	10.1016/s1389-1723(99)80103-0	1999	*
Metabolism	A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil.	Luz AP, Pellizari VH, Whyte LG, Greer CW	Can J Microbiol	10.1139/w04-008	2004	*
Phylogeny	Detection of catabolic genes in indigenous microbial consortia isolated from a diesel-contaminated soil.	Milcic-Terzic J, Lopez-Vidal Y, Vrvic MM, Saval S	Bioresour Technol	10.1016/s0960-8524(00)00156-5	2001	*
Metabolism	Adaptation of Pseudomonas putida mt-2 to growth on aromatic amines.	McClure NC, Venables WA	J Gen Microbiol	10.1099/00221287-132-8-2209	1986	*
Genetics	Genomotyping of Pseudomonas putida strains using P. putida KT2440-based high-density DNA microarrays: implications for transcriptomics studies.	Ballerstedt H, Volkers RJ, Mars AE, Hallsworth JE, dos Santos VA, Puchalka J, van Duuren J, Eggink G, Timmis KN, de Bont JA, Wery J	Appl Microbiol Biotechnol	10.1007/s00253-007-0914-z	2007	*
	Development of a membrane-based vapor-phase bioreactor.	Rouhana N, Handagama N, Bienkowski PR	Appl Biochem Biotechnol	10.1007/BF02920477	1997	*
	Fate of Pseudomonas putida after release into lake water mesocosms: Different survival mechanisms in response to environmental conditions.	Brettar I, Ramos-Gonzalez MI, Ramos JL, Hofle MG	Microb Ecol	10.1007/BF00165812	1994	*
	Radiometric determination of uranium accumulated in bacterial cells.	Chwistek M, Chmielowski J, Tomza I	Talanta	10.1016/0039-9140(88)80070-5	1988	*
	Binding of Germanium to Pseudomonas putida Cells.	Klapcinska B, Chmielowski J	Appl Environ Microbiol	10.1128/aem.51.5.1144-1147.1986	1986	*
	Bioaccumulation of Germanium by Pseudomonas putida in the Presence of Two Selected Substrates.	Chmielowski J, Klapcinska B	Appl Environ Microbiol	10.1128/aem.51.5.1099-1103.1986	1986	*

References

#1518

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3931

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#44546 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 2480
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82092

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92520.1 )

#82093

* These data were automatically processed and therefore are not curated

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