Name: Pseudomonas putida cl-B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12921

Type strain:

Species: Pseudomonas putida

Strain Designation: cl-B

Culture col. no.: DSM 50198, ATCC 17453, ICPB 2563-77, JCM 6157, KCTC 1646

Strain history: ATCC 17453 <-- R. Y. Stanier 77 <-- I. C. Gunsalus C 1-B.

NCBI tax ID(s): 303 (species)

Other strains from Pseudomonas putida

Section

Pseudomonas putida cl-B is a mesophilic human pathogen that was isolated from soil.

human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (PNU)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12675]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #12675]

Culture medium growth

[Ref.: #12675]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #12675]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #12675]			growth	26 °C
[Ref.: #67770]			growth	26 °C

[Ref.: #12675]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #12675]	20325	-	-	-	+	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12675]	Sample type/isolated from	soil
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF094746 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	543
Soil Samples	368
Animal Samples	799
Plant Samples	423
Total Samples	2133

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12675]	Pathogenicity (human)	yes
[Ref.: #12675]	Pathogenicity (animal)	yes
[Ref.: #12675]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Pseudomonas putida strain ATCC 17453 16S ribosomal RNA gene, partial sequence	AF094746	1475	ENA	303 tax ID	*
[Ref.: #20218]	Pseudomonas putida strain ATCC 17453 16S ribosomal RNA gene, partial sequence	JN630891	791	ENA	303 tax ID	*

Availability in culture collections External links

[Ref.: #12675]

Culture collection no.

DSM 50198, ATCC 17453, ICPB 2563-77, JCM 6157, KCTC 1646

[Ref.: #20218]

Associated Passport(s) in StrainInfo

170734, 99189, 170735

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Microbial oxidation of adamantanone by Pseudomonas putida carrying the camphor catabolic plasmid.	Selifonov SA	Biochem Biophys Res Commun	10.1016/s0006-291x(05)81566-9	1992	*
Enzymology	Nucleotide sequence of the Pseudomonas putida cytochrome P-450cam gene and its expression in Escherichia coli.	Unger BP, Gunsalus IC, Sligar SG	J Biol Chem	S0021-9258(17)36068-4	1986	*
Metabolism	Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453.	Taylor DG, Trudgill PW	J Bacteriol	10.1128/jb.165.2.489-497.1986	1986	*
Metabolism	Evidence for autoregulation of camR, which encodes a repressor for the cytochrome P-450cam hydroxylase operon on the Pseudomonas putida CAM plasmid.	Aramaki H, Sagara Y, Hosoi M, Horiuchi T	J Bacteriol	10.1128/jb.175.24.7828-7833.1993	1993	*
Enzymology	Complete nucleotide sequence of the 5-exo-hydroxycamphor dehydrogenase gene on the CAM plasmid of Pseudomonas putida (ATCC 17453).	Aramaki H, Koga H, Sagara Y, Hosoi M, Horiuchi T	Biochim Biophys Acta	10.1016/0167-4781(93)90098-x	1993	*
Metabolism	Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453.	Jones KH, Smith RT, Trudgill PW	J Gen Microbiol	10.1099/00221287-139-4-797	1993	*
Enzymology	Characterization of cytochrome P450 monooxygenase CYP154H1 from the thermophilic soil bacterium Thermobifida fusca.	Schallmey A, den Besten G, Teune IG, Kembaren RF, Janssen DB	Appl Microbiol Biotechnol	10.1007/s00253-010-2965-9	2010	*
Enzymology	Cloning, Baeyer-Villiger biooxidations, and structures of the camphor pathway 2-oxo-Delta(3)-4,5,5-trimethylcyclopentenylacetyl-coenzyme A monooxygenase of Pseudomonas putida ATCC 17453.	Leisch H, Shi R, Grosse S, Morley K, Bergeron H, Cygler M, Iwaki H, Hasegawa Y, Lau PC	Appl Environ Microbiol	10.1128/AEM.07694-11	2012	*
Enzymology	Camphor pathway redux: functional recombinant expression of 2,5- and 3,6-diketocamphane monooxygenases of Pseudomonas putida ATCC 17453 with their cognate flavin reductase catalyzing Baeyer-Villiger reactions.	Iwaki H, Grosse S, Bergeron H, Leisch H, Morley K, Hasegawa Y, Lau PC	Appl Environ Microbiol	10.1128/AEM.03958-12	2013	*
Metabolism	Biodegradation of 1-allyloxy-4-propoxybenzene by selected strains of Pseudomonas putida.	Ebrahimi P, Plettner E	Biodegradation	10.1007/s10532-013-9638-1	2013	*
Enzymology	Multiple native flavin reductases in camphor-metabolizing Pseudomonas putida NCIMB 10007: functional interaction with two-component diketocamphane monooxygenase isoenzymes.	Willetts A, Kelly DR	Microbiology (Reading)	10.1099/mic.0.079913-0	2014	*
Metabolism	The production of omega-hydroxy palmitic acid using fatty acid metabolism and cofactor optimization in Escherichia coli.	Sung C, Jung E, Choi KY, Bae JH, Kim M, Kim J, Kim EJ, Kim PI, Kim BG	Appl Microbiol Biotechnol	10.1007/s00253-015-6630-1	2015	*
Metabolism	Chemotaxis by Pseudomonas putida (ATCC 17453) towards camphor involves cytochrome P450cam (CYP101A1).	Balaraman P, Plettner E	Biochim Biophys Acta Gen Subj	10.1016/j.bbagen.2018.10.018	2018	*
Enzymology	Conferring the Metabolic Self-Sufficiency of the CAM Plasmid of Pseudomonas putida ATCC 17453: The Key Role of Putidaredoxin Reductase.	Willetts A	Microorganisms	10.3390/microorganisms7100395	2019	*
	Characterised Flavin-Dependent Two-Component Monooxygenases from the CAM Plasmid of Pseudomonas putida ATCC 17453 (NCIMB 10007): ketolactonases by Another Name.	Willetts A	Microorganisms	10.3390/microorganisms7010001	2018	*
	The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453-An Episodic History and Still Mysterious after 60 Years.	Willetts A	Microorganisms	10.3390/microorganisms9122593	2021	*

References

#12675

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 50198

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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