Name: Pseudomonas putida B6-2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 24385

Type strain:

Species: Pseudomonas putida

Strain Designation: B6-2

Culture col. no.: DSM 28064

Strain history: <- P. Xu, Shanghai Jiao Tong Univ., China; B6-2

NCBI tax ID(s): 303 (species)

SI-ID 407704

DSM 28064

Special Collections

DSMZ

Other strains from Pseudomonas putida

Section

Pseudomonas putida B6-2 is a Gram-negative human pathogen that was isolated from biphenyl contaminated soil.

Gram-negative
human pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (LPSN)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99

Multimedia content

[Ref.: #20320]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20320]

Culture medium

MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)

[Ref.: #20320]

Culture medium growth

[Ref.: #20320]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20320]

Culture medium composition

expand / minimize

[Ref.: #20320]

Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

[Ref.: #20320]

Culture medium growth

[Ref.: #20320]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20320]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #20320]			growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Oxygen tolerance

aerobe Confidence in %93.033

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20320]	Sample type/isolated from	biphenyl contaminated soil
[Ref.: #20320]	Geographic location (country and/or sea, region)	Shandong Province, Jinan
[Ref.: #20320]	Country	China
[Ref.: #20320]	Country ISO 3 Code	CHN
[Ref.: #20320]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Contamination	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF672104 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	543
Soil Samples	368
Animal Samples	799
Plant Samples	423
Total Samples	2133

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20320]	Pathogenicity (human)	yes
[Ref.: #20320]	Pathogenicity (animal)	yes
[Ref.: #20320]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20320]	Pseudomonas sp. B6-2 16S ribosomal RNA gene, partial sequence	EF672104	1402	GenBank ENA	453577 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas putida B6-2	GCA_000226035	complete	GenBank ENA	1081940 tax ID	*
[Ref.: #66792]	Pseudomonas putida B6-2	1081940.5	wgs	PATRIC	1081940 tax ID	*
[Ref.: #66792]	Pseudomonas putida B6-2	2548876715	complete	JGI IMG/M	1081940 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas putida B6-2 B6-2 NCBI: GCA_000226035.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	99.062	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	93.033	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	85.94	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	98.986	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	89.019	no

Availability in culture collections External links

[Ref.: #20320]

Culture collection no.

DSM 28064

[Ref.: #88295]

SI-ID 407704

Literature:

	Title	Authors	Journal	DOI	Year
Metabolism	Unique regulator SrpR mediates crosstalk between efflux pumps TtgABC and SrpABC in Pseudomonas putida B6-2 (DSM 28064).	Yao X, Tao F, Tang H, Hu H, Wang W, Xu P	Mol Microbiol	10.1111/mmi.14605	2020	*
Metabolism	Multiple Roles for Two Efflux Pumps in the Polycyclic Aromatic Hydrocarbon-Degrading Pseudomonas putida Strain B6-2 (DSM 28064).	Yao X, Tao F, Zhang K, Tang H, Xu P	Appl Environ Microbiol	10.1128/AEM.01882-17	2017	*
Genetics	Genome sequence of Pseudomonas putida strain B6-2, a superdegrader of polycyclic aromatic hydrocarbons and dioxin-like compounds.	Tang H, Yu H, Li Q, Wang X, Gai Z, Yin G, Su F, Tao F, Ma C, Xu P	J Bacteriol	10.1128/JB.06201-11	2011	*
Metabolism	New metabolites in dibenzofuran cometabolic degradation by a biphenyl-cultivated Pseudomonas putida strain B6-2.	Li Q, Wang X, Yin G, Gai Z, Tang H, Ma C, Deng Z, Xu P	Environ Sci Technol	10.1021/es901991d	2009	*
	Genetic mapping of highly versatile and solvent-tolerant Pseudomonas putida B6-2 (ATCC BAA-2545) as a 'superstar' for mineralization of PAHs and dioxin-like compounds.	Wang W, Li Q, Zhang L, Cui J, Yu H, Wang X, Ouyang X, Tao F, Xu P, Tang H	Environ Microbiol	10.1111/1462-2920.15613	2021	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20320

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28064

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#88295

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407704.1 )

* These data were automatically processed and therefore are not curated

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Pseudomonas putida B6-2 B6-2

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