Name: Pseudomonas putida 77
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12874

Type strain:

Species: Pseudomonas putida

Strain Designation: 77

Culture col. no.: DSM 1693, ATCC 8209, CCM 380, IFO 3738, NBRC 3738, NCIB 8296, CCUG 555, JCM 20511, BUCSAV 290, CCEB 380, CCM 1977, CIP 59.17, IAM 12355, LMG 2232, NCIMB 8296, NRRL B-1595, CIP 63.50

Strain history: CIP <- 1959, NCIB <- N.R. Smith: strain 77, Pseudomonas ovalis

NCBI tax ID(s): 303 (species)

SI-ID 4216

LMG 2232, ATCC 8209, CCM 1977, CCUG 555, DSM 1693, IAM 12355, IFO 3738, NCIB 8296, NRRL B-1595, CCM 380, NCIMB 8296, VKM B-899, CCRC 11903, CIP 59.17, CIP 63.50, KCTC 2349, NBRC 3738, BCRC 11903, NBIMCC 1133, NCIM 2101, CGMCC 1.2309, CFBP 3141, JCM 20511

Special Collections

Other strains from Pseudomonas putida

Section

Pseudomonas putida 77 is an obligate aerobe, Gram-negative, motile human pathogen that was isolated from Unknown source.

Gram-negative
motile
rod-shaped
human pathogen
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (LPSN)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on morphological and physiological properties Morphology

[Ref.: #122069]	Gram stain	negative

[Ref.: #122069]	Cell shape	rod-shaped

[Ref.: #122069]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #863]

Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

[Ref.: #863]

Culture medium growth

[Ref.: #863]

Culture medium link

Medium recipe at MediaDive

[Ref.: #863]

Culture medium composition

expand / minimize

[Ref.: #36969]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #36969]

Culture medium growth

[Ref.: #36969]

Culture medium composition

expand / minimize

[Ref.: #122069]

Culture medium

CIP Medium 3

[Ref.: #122069]

Culture medium growth

[Ref.: #122069]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122069]

Culture medium

CIP Medium 72

[Ref.: #122069]

Culture medium growth

[Ref.: #122069]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #863]	growth	26 °C
[Ref.: #36969]	growth	30 °C
[Ref.: #67770]	growth	25 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122069]

Oxygen tolerance

obligate aerobe

[Ref.: #863]

Name of produced compound

ketogluconic acid

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #122069]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #122069]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #122069]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122069]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122069]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #122069]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44230]

Fatty acid	Percentage	ECL
C16:0	27.1	16
C16:1 ω7c	25.9	15.819
C18:1 ω7c /12t/9t	16.7	17.824
C17:0 CYCLO	9.2	16.888
C12:0	6	12
C12:0 3OH	4.4	13.455
C12:0 2OH	3.7	13.178
C10:0 3OH	3.6	11.423
C15:0	0.9	15
C14:0	0.8	14
C17:0	0.7	17
C17:1 ω8c	0.5	16.792
C19:0 CYCLO ω8c	0.5	18.9

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #863]	22810	-	-	-	+	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122069]	12950	-	+	+	+	+	+	+	-	+	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #122069]	Sample type/isolated from	Unknown source
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence AB680123 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	2210
Soil Samples	1775
Animal Samples	2599
Plant Samples	866
Total Samples	7450

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #863]	Pathogenicity (human)	yes
[Ref.: #863]	Pathogenicity (animal)	yes
[Ref.: #863]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #122069]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Pseudomonas putida strain ATCC 8209 clone 1 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	DQ095205	754	GenBank ENA	303 tax ID	*
[Ref.: #20218]	Pseudomonas putida strain ATCC 8209 clone 2 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	DQ095206	754	GenBank ENA	303 tax ID	*
[Ref.: #20218]	Pseudomonas putida strain ATCC 8209 clone 3 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer and tRNA-Glu gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	DQ095207	668	GenBank ENA	303 tax ID	*
[Ref.: #20218]	Pseudomonas putida 16S ribosomal RNA gene, strain: IFO 3738, partial sequence	D85999	1329	GenBank ENA	303 tax ID	*
[Ref.: #20218]	Pseudomonas putida gene for 16S rRNA, partial sequence, strain: NBRC 3738	AB680123	1462	GenBank ENA	303 tax ID	*

Availability in culture collections External links

[Ref.: #863]

Culture collection no.

DSM 1693, ATCC 8209, CCM 380, IFO 3738, NBRC 3738, NCIB 8296, CCUG 555, JCM 20511, BUCSAV 290, CCEB 380, CCM 1977, CIP 59.17, IAM 12355, LMG 2232, NCIMB 8296, NRRL B-1595, CIP 63.50

[Ref.: #82086]

SI-ID 4216

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Microbial asymmetric oxidation of 2-butyl-1,3-propanediol.	Mitsukura K, Uno T, Yoshida T, Nagasawa T	Appl Microbiol Biotechnol	10.1007/s00253-007-0974-0	2007	*
Enzymology	Cloning of a gene for D-sorbitol dehydrogenase from Gluconobacter oxydans G624 and expression of the gene in Pseudomonas putida IFO3738.	Shibata T, Ichikawa C, Matsuura M, Takata Y, Noguchi Y, Saito Y, Yamashita M	J Biosci Bioeng	10.1016/s1389-1723(00)89097-0	2000	*
Biotechnology	Metabolic engineering study on the direct fermentation of 2-keto-L-gulonic acid, a key intermediate of L-ascorbic acid in Pseudomonas putida IFO3738.	Shibata T, Ichikawa C, Matsuura M, Takata Y, Noguchi Y, Saito Y, Yamashita M	J Biosci Bioeng	S1389-1723(00)80116-4	2000	*
Enzymology	Purification of bacterial L-methionine gamma-lyase.	Nakayama T, Esaki N, Sugie K, Beresov TT, Tanaka H, Soda K	Anal Biochem	10.1016/0003-2697(84)90832-7	1984	*

References

#863	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1693

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36969 ; Curators of the CIP;
#44230 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 555
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82086

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID4216.1 )

#122069 Collection of Institut Pasteur ; Curators of the CIP; CIP 59.17
* These data were automatically processed and therefore are not curated

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