Pseudomonas putida (DSM 1693, ATCC 8209, CCM 380)

Name: Pseudomonas putida (DSM 1693, ATCC 8209, CCM 380)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12874

Type strain

Strain Designation 77

Culture col. no. DSM 1693 ATCC 8209 CCM 380 IFO 3738 NBRC 3738 13 more

NCBI tax ID(s) 303

Special Collections DSMZ CCUG JCM CIP

History <- NCIB <- ATCC <- N.R. Smith; 77 (Pseudomonas ovalis) IAM 12355 <-- IFO 3738 <-- BUCSAV 290 <-- NCIB 8296 <-- ATCC 8209 <-- N. R. Smith NRS 77. CIP <- 1959, NCIB <- N.R. Smith: strain 77, Pseudomonas ovalis

Links

Pseudomonas putida 77 is an obligate aerobe, Gram-negative, motile human pathogen of the family Pseudomonadaceae.

Gram-negative motile rod-shaped obligate aerobe human pathogen 16S sequence Bacteria

Name and taxonomic classification

SI-ID 4216

LMG 2232,ATCC 8209,CCM 1977,CCUG 555,DSM 1693,IAM 12355,IFO 3738,NCIB 8296,NRRL B-1595,CCM 380,NCIMB 8296,VKM B-899,CCRC 11903,CIP 59.17,CIP 63.50,KCTC 2349,NBRC 3738,BCRC 11903,NBIMCC 1133,NCIM 2101,CGMCC 1.2309,CFBP 3141,JCM 20511

@ref 20215

Last LPSN update 2026-02-09 11:20:57

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas putida

Full scientific name Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

Synonyms (2)

Arthrobacter siderocapsulatus
"Bacillus putidus"

BacDive ID	Other strains from **Pseudomonas putida** (130)	Type strain
12871	P. putida 90, A 3.12, DSM 291, ATCC 12633, DSM 50202, ... (type strain)
12872	P. putida 1593, DSM 295
12873	P. putida C-1, DSM 437, ATCC 23974, DSM 50253
12875	P. putida 12/5, DSM 1868
12876	P. putida PaW 340, DSM 2112
12877	P. putida DSM 3226
12880	P. putida mt-2 PaW1, pWWO, DSM 3931, ATCC 33015, NCIB ...
12881	P. putida MT20, pWW20, DSM 3932, NCIB 12186
12882	P. putida MT15, pWW15, DSM 3936, NCIB 12184
12883	P. putida PaW611, pWW53-4, DSM 3937
12884	P. putida PaW701, pWW60-1, DSM 3938, NCIB 12198
12885	P. putida KF439, pKF439, DSM 4011
12886	P. putida PaW736, pWW60-22, DSM 4302, NCIB 12199
12887	P. putida MT53, pWW53, DSM 4303, NCIB 12187
12888	P. putida CAM, PpG1, DSM 4475
12889	P. putida NAH, PpG7, DSM 4476
12890	P. putida NIC, PpG25, DSM 4477
12891	P. putida PpG2100, SAL, DSM 4478
12892	P. putida PaW 130, DSM 5169
12893	P. putida PaW 95, DSM 5170
12894	P. putida CLB250, DSM 5232
12895	P. putida DSM 84, IMG 1513
12896	P. putida mt-2 KT2440, DSM 6125, ATCC 47054, CCUG 56301, ...
12898	P. putida S-832, DSM 6883
12899	P. putida S-313, DSM 6884
12900	P. putida BG1, DSM 6413
12901	P. putida DSM 6521, ATCC 11172, NCIB 8251, JCM 13061, ...
12902	P. putida pUTK9, DSM 6701, RB 1351
12903	P. putida US2, DSM 6755
12904	P. putida F1, PpF1, DSM 6899, ATCC 700007, JCM 31910, ...
12906	P. putida PS, DSM 7189
12907	P. putida Strain G, BKM-BN1123, DSM 7286, BKM BN-1123, ...
12908	P. putida Strain A, DSM 7314, NCIMB 11286, VKM B-1122, ...
12909	P. putida MM1, DSM 9278
12910	P. putida RW20-3b, DSM 11738
12911	P. putida GS1, DSM 12264
12912	P. putida 2-2L, DSM 12585
12913	P. putida PNP1, DSM 12448
12914	P. putida U, DSM 12735, ATCC 17514, NCIMB 10015, CCUG ...
12915	P. putida DSM 13624, IMI 358533
12918	P. putida TM1, DSM 16845
12919	P. putida 50-3-3, IBPM B-20, DSM 46608, CCEB 520, CIP ...
12920	P. putida Berlin 33/2, DSM 50026, CIP 103281
12921	P. putida cl-B, DSM 50198, ATCC 17453, ICPB 2563-77, ...
12922	P. putida 53-2, DSM 50208, ATCC 17485, ICPB 2789-111, ...
12924	P. putida DSM 50257, ATCC 6876, DSM 60118, NCPPB 244, ...
12925	P. putida MT-I1, DSM 21680
12926	P. putida WB, DSM 23849
100159	P. putida ST033293(HKI),
24382	P. putida 160, DSM 50194, ATCC 17529, ICPB 2440-133
24383	P. putida MS of E.E. Snell, DSM 50204, ATCC 17457, ...
24384	P. putida S16, DSM 28022
24385	P. putida B6-2, DSM 28064
130567	P. putida SQ1, DSM 100120
130999	P. putida Z2, DSM 16527
131000	P. putida TEC1, DSM 26234
131001	P. putida TEC1 407.1-delta 2, DSM 26250
131002	P. putida TEC1 407.3-delta2, DSM 26251
131003	P. putida TEC1 407-delta1, DSM 26252
131888	P. putida S13.1.2, DSM 102034
132784	P. putida TEC1 91-delta 1, DSM 26253
134429	P. putida AmMs 106, CIP 104063, ATCC 49128
134979	P. putida CIP 55.5, ATCC 795, IAM 1505, NCDO 754, NCIMB ...
135611	P. putida CIP 59.25, ATCC 12842, NCIMB 8865, JCM 13060, ...
135612	P. putida A3/9, CIP 59.26, NCIMB 8248
135753	P. putida CIP 108916, LMG 1246, ATCC 17430, NCIMB 10534, ...
135983	P. putida CIP 52.195
135984	P. putida CIP 52.196
136153	P. putida CIP 52.193
136154	P. putida CIP 52.197
136155	P. putida CIP 52.198
137196	P. putida CIP 52.194
137217	P. putida CIP 52.190
137776	P. putida 25300, 642-85, CIP 101794
137973	P. putida 340-86, CIP 102341
138643	P. putida 776.85, CIP 101936
138858	P. putida MD 7.3, CIP 59.19, NCIMB 8729
139223	P. putida CIP 52.189
141228	P. putida CCUG 264
141276	P. putida CCUG 553, LMG 2189
141504	P. putida CCUG 2471
141507	P. putida CCUG 2479
141521	P. putida CCUG 2525, NRRL B-2022
142893	P. putida CCUG 15619
143393	P. putida CCUG 18875
144913	P. putida CCUG 27052
145592	P. putida CCUG 28920
145805	P. putida CCUG 29643
146210	P. putida CCUG 30612
146426	P. putida CCUG 31110
146710	P. putida CCUG 32007
147231	P. putida CCUG 33113
147438	P. putida CCUG 33582
147515	P. putida CCUG 33721
147793	P. putida CCUG 34242
148510	P. putida CCUG 35570
149198	P. putida CCUG 37253
150737	P. putida CCUG 42126
153432	P. putida CCUG 49576
153483	P. putida CCUG 49716
153616	P. putida CCUG 50315
153618	P. putida CCUG 50317
154720	P. putida CCUG 54971
155478	P. putida CCUG 57833
156241	P. putida CCUG 60837
156461	P. putida CCUG 61918
161309	P. putida JCM 13062, ATCC 11250, CCM 3656, KCTC 1645, ...
161475	P. putida JCM 14351, ATCC 598, NRRL B-251
162298	P. putida JCM 18798
162685	P. putida JCM 20004, IAM 1002
162686	P. putida JCM 20005, IAM 1003
162700	P. putida JCM 20028, IAM 1049
162701	P. putida JCM 20029, IAM 1050
162713	P. putida JCM 20048, IAM 1094
162723	P. putida JCM 20066, IAM 1128
162741	P. putida JCM 20089, IAM 1177
162752	P. putida JCM 20102, IAM 1200
162760	P. putida JCM 20111, IAM 1218
162761	P. putida JCM 20112, ATCC 17464, IAM 1219
162768	P. putida JCM 20120, IAM 1235
162775	P. putida JCM 20129, IAM 1250
162804	P. putida JCM 20188, ATCC 4359, IAM 1506
162815	P. putida JCM 20228, IAM 1629
165987	P. putida JCM 9045
166071	P. putida JCM 9800
166073	P. putida JCM 9802
174606	P. putida Thor-Nr. 1021, DSM 115323
174607	P. putida 116234, DSM 116234
174608	P. putida 116510, DSM 116510
176535	P. putida NiuAA7, DSM 114486

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
122069	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
863	REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)	Medium recipe at MediaDive	Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36969	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
122069	CIP Medium 3	Medium recipe at CIP
122069	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
863	positive	growth	26
36969	positive	growth	30
67770	positive	growth	25

Physiology and metabolism

Oxygen tolerance

122069

Oxygen toleranceobligate aerobe

Compound production

863

Compoundketogluconic acid

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
122069	17632 ChEBI	nitrate	-	reduction
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
122069	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
122069	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122069	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122069	oxidase	+
68382	trypsin	+	3.4.21.4	from API zym
122069	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	+		from API zym

Fatty acid profile

Metadata FA analysis

@ref

44230

Fatty acid	Percentage	ECL
C16:0	27.1	16
C16:1 ω7c	25.9	15.819
C18:1 ω7c /12t/9t	16.7	17.824
C17:0 CYCLO	9.2	16.888
C12:0	6	12
C12:0 3OH	4.4	13.455
C12:0 2OH	3.7	13.178
C10:0 3OH	3.6	11.423
C15:0	0.9	15
C14:0	0.8	14
C17:0	0.7	17
C17:1 ω8c	0.5	16.792
C19:0 CYCLO ω8c	0.5	18.9

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122069	-	+	+	+	+	+	+	-	+	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
863	-	-	-	+	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	+	+

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence AB680123 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	2210
Soil Samples	1775
Animal Samples	2599
Plant Samples	866
Total Samples	7450

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
863			2	Risk group (German classification) According to TRBA 466
122069			1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Pseudomonas putida gene for 16S rRNA, partial sequence, strain: NBRC 3738	AB680123	1462	303
20218	Pseudomonas putida 16S ribosomal RNA gene, strain: IFO 3738, partial sequence	D85999	1329	303
124043	Pseudomonas putida strain ATCC 8209 16S ribosomal RNA gene, partial sequence.	PQ130041	918	303

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Herellea (Acinetobacter) and Pseudomonas ovalis (P. putida) from frozen foods.	Eller C.	Appl Microbiol	10.1128/am.17.1.26-30.1969	1969
	The oxazolomycin family: a review of current knowledge.	Oleksak P, Gonda J, Nepovimova E, Kuca K, Musilek K.	RSC Adv	10.1039/d0ra08396h	2020
Metabolism	L-sorbose reductase and its transcriptional regulator involved in L-sorbose utilization of Gluconobacter frateurii.	Soemphol W, Toyama H, Moonmangmee D, Adachi O, Matsushita K.	J Bacteriol	10.1128/jb.01895-06	2007
Metabolism	Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcohols.	Toyama H, Fujii A, Matsushita K, Shinagawa E, Ameyama M, Adachi O.	J Bacteriol	10.1128/jb.177.9.2442-2450.1995	1995
Enzymology	NADP(+)-dependent D-threonine dehydrogenase from Pseudomonas cruciviae IFO 12047.	Misono H, Kato I, Packdibamrung K, Nagata S, Nagasaki S.	Appl Environ Microbiol	10.1128/aem.59.9.2963-2968.1993	1993
Genetics	Genomic Reclassification and Phenotypic Characterization of Pseudomonas putida Strains Deposited in Japanese Culture Collections.	Morohoshi T, Yaguchi N, Someya N.	Microbes Environ	10.1264/jsme2.me23019	2023
Enzymology	Investigation of persistent colonization by Pseudomonas aeruginosa-like strains in a spring water bottling plant.	Morais PV, Mesquita C, Andrade JL, da Costa MS.	Appl Environ Microbiol	10.1128/aem.63.3.851-856.1997	1997
Metabolism	Microbial asymmetric oxidation of 2-butyl-1,3-propanediol.	Mitsukura K, Uno T, Yoshida T, Nagasawa T	Appl Microbiol Biotechnol	10.1007/s00253-007-0974-0	2007
Enzymology	Cloning of a gene for D-sorbitol dehydrogenase from Gluconobacter oxydans G624 and expression of the gene in Pseudomonas putida IFO3738.	Shibata T, Ichikawa C, Matsuura M, Takata Y, Noguchi Y, Saito Y, Yamashita M	J Biosci Bioeng	10.1016/s1389-1723(00)89097-0	2000
Biotechnology	Metabolic engineering study on the direct fermentation of 2-keto-L-gulonic acid, a key intermediate of L-ascorbic acid in Pseudomonas putida IFO3738.	Shibata T, Ichikawa C, Matsuura M, Takata Y, Noguchi Y, Saito Y, Yamashita M	J Biosci Bioeng	S1389-1723(00)80116-4	2000
Enzymology	Purification of bacterial L-methionine gamma-lyase.	Nakayama T, Esaki N, Sugie K, Beresov TT, Tanaka H, Soda K	Anal Biochem	10.1016/0003-2697(84)90832-7	1984

References

#863	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1693
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#36969	; Curators of the CIP;
#44230	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 555
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122069	Collection of Institut Pasteur ; Curators of the CIP; CIP 59.17
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas putida (DSM 1693, ATCC 8209, CCM 380)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

API zym

API 20NE

Isolation, sampling and environmental information

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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