Strain identifier

BacDive ID: 12874

Type strain: No

Species: Pseudomonas putida

Strain Designation: 77

Strain history: CIP <- 1959, NCIB <- N.R. Smith: strain 77, Pseudomonas ovalis

NCBI tax ID(s): 303 (species)

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General

@ref: 863

BacDive-ID: 12874

DSM-Number: 1693

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Pseudomonas putida 77 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from Unknown source.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

@refhistory
863<- NCIB <- ATCC <- N.R. Smith; 77 (Pseudomonas ovalis)
67770IAM 12355 <-- IFO 3738 <-- BUCSAV 290 <-- NCIB 8296 <-- ATCC 8209 <-- N. R. Smith NRS 77.
122069CIP <- 1959, NCIB <- N.R. Smith: strain 77, Pseudomonas ovalis

doi: 10.13145/bacdive12874.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 863

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

strain designation: 77

type strain: no

Morphology

cell morphology

  • @ref: 122069
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
863REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36969MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
122069CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
122069CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
863positivegrowth26mesophilic
36969positivegrowth30mesophilic
67770positivegrowth25mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122069
  • oxygen tolerance: obligate aerobe

compound production

  • @ref: 863
  • compound: ketogluconic acid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12206917632nitrate-reduction
12206916301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12206935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
122069oxidase+
122069catalase+1.11.1.6
122069urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44230C12:0612
    44230C14:00.814
    44230C15:00.915
    44230C16:027.116
    44230C17:00.717
    44230C10:0 3OH3.611.423
    44230C12:0 2OH3.713.178
    44230C12:0 3OH4.413.455
    44230C16:1 ω7c25.915.819
    44230C17:0 CYCLO9.216.888
    44230C17:1 ω8c0.516.792
    44230C18:1 ω7c /12t/9t16.717.824
    44230C19:0 CYCLO ω8c0.518.9
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122069-++++++-+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
863---+----+-----++-++++

Isolation, sampling and environmental information

isolation

  • @ref: 122069
  • sample type: Unknown source

taxonmaps

  • @ref: 69479
  • File name: preview.99_953.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_642;99_953&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AB680123
  • Sequence Identity:
  • Total samples: 7450
  • soil counts: 1775
  • aquatic counts: 2210
  • animal counts: 2599
  • plant counts: 866

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
863yesyes2Risk group (German classification)
1220691Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas putida gene for 16S rRNA, partial sequence, strain: NBRC 3738AB6801231462ena303
20218Pseudomonas putida 16S ribosomal RNA gene, strain: IFO 3738, partial sequenceD859991329ena303
20218Pseudomonas putida strain ATCC 8209 clone 1 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceDQ095205754ena303
20218Pseudomonas putida strain ATCC 8209 clone 2 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceDQ095206754ena303
20218Pseudomonas putida strain ATCC 8209 clone 3 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer and tRNA-Glu gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceDQ095207668ena303

External links

@ref: 863

culture collection no.: DSM 1693, ATCC 8209, CCM 380, IFO 3738, NBRC 3738, NCIB 8296, CCUG 555, JCM 20511, BUCSAV 290, CCEB 380, CCM 1977, CIP 59.17, IAM 12355, LMG 2232, NCIMB 8296, NRRL B-1595, CIP 63.50

straininfo link

  • @ref: 82086
  • straininfo: 4216

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology6742420Purification of bacterial L-methionine gamma-lyase.Nakayama T, Esaki N, Sugie K, Beresov TT, Tanaka H, Soda KAnal Biochem10.1016/0003-2697(84)90832-71984Carbon-Sulfur Lyases/*isolation & purification/metabolism, Chromatography, Ion Exchange, Crystallization, Kinetics, Lyases/*isolation & purification, Molecular Weight, Pseudomonas/*enzymology, Pyridoxal Phosphate/analysis, Spectrophotometry, Substrate SpecificityPhylogeny
Enzymology16232778Cloning of a gene for D-sorbitol dehydrogenase from Gluconobacter oxydans G624 and expression of the gene in Pseudomonas putida IFO3738.Shibata T, Ichikawa C, Matsuura M, Takata Y, Noguchi Y, Saito Y, Yamashita MJ Biosci Bioeng10.1016/s1389-1723(00)89097-02000
Biotechnology16232848Metabolic engineering study on the direct fermentation of 2-keto-L-gulonic acid, a key intermediate of L-ascorbic acid in Pseudomonas putida IFO3738.Shibata T, Ichikawa C, Matsuura M, Takata Y, Noguchi Y, Saito Y, Yamashita MJ Biosci BioengS1389-1723(00)80116-42000
Metabolism17453192Microbial asymmetric oxidation of 2-butyl-1,3-propanediol.Mitsukura K, Uno T, Yoshida T, Nagasawa TAppl Microbiol Biotechnol10.1007/s00253-007-0974-02007Acetobacter/*metabolism, Biotransformation, Oxidation-Reduction, Propylene Glycol/*metabolism, Pseudomonas putida/*metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
863Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1693)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1693
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36969Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9757
44230Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 555)https://www.ccug.se/strain?id=555
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82086Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4216.1StrainInfo: A central database for resolving microbial strain identifiers
122069Curators of the CIPCollection of Institut Pasteur (CIP 59.17)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2059.17