Pseudomonas putida (DSM 6521, ATCC 11172, NCIB 8251)

Name: Pseudomonas putida (DSM 6521, ATCC 11172, NCIB 8251)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12901

Type strain

Culture col. no. DSM 6521 ATCC 11172 NCIB 8251 NCIMB 8251 NCCB 92006 3 more

NCBI tax ID(s) 303

Special Collections DSMZ JCM

History <- ATCC <- NCIB (Pseudomonas fluorescens) <- W. Evans K. Komagata <-- R. Hugh 821 <-- ATCC 11172 <-- NCIMB 8251 <-- W. C. Evans.

Links

Pseudomonas putida DSM 6521 is a human pathogen of the family Pseudomonadaceae.

human pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 35334

ATCC 11172,NCIB 8251,DSM 6521,NCIMB 8251,NCCB 92006,LMD 92.6,BCRC 14349,CCRC 14349,NCAIM B.01522,JCM 13061,ATCC 11772

@ref 20215

Last LPSN update 2026-02-09 11:20:58

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas putida

Full scientific name Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

Synonyms (2)

Arthrobacter siderocapsulatus
"Bacillus putidus"

BacDive ID	Other strains from **Pseudomonas putida** (130)	Type strain
12871	P. putida 90, A 3.12, DSM 291, ATCC 12633, DSM 50202, ... (type strain)
12872	P. putida 1593, DSM 295
12873	P. putida C-1, DSM 437, ATCC 23974, DSM 50253
12874	P. putida 77, DSM 1693, ATCC 8209, CCM 380, IFO 3738, ...
12875	P. putida 12/5, DSM 1868
12876	P. putida PaW 340, DSM 2112
12877	P. putida DSM 3226
12880	P. putida mt-2 PaW1, pWWO, DSM 3931, ATCC 33015, NCIB ...
12881	P. putida MT20, pWW20, DSM 3932, NCIB 12186
12882	P. putida MT15, pWW15, DSM 3936, NCIB 12184
12883	P. putida PaW611, pWW53-4, DSM 3937
12884	P. putida PaW701, pWW60-1, DSM 3938, NCIB 12198
12885	P. putida KF439, pKF439, DSM 4011
12886	P. putida PaW736, pWW60-22, DSM 4302, NCIB 12199
12887	P. putida MT53, pWW53, DSM 4303, NCIB 12187
12888	P. putida CAM, PpG1, DSM 4475
12889	P. putida NAH, PpG7, DSM 4476
12890	P. putida NIC, PpG25, DSM 4477
12891	P. putida PpG2100, SAL, DSM 4478
12892	P. putida PaW 130, DSM 5169
12893	P. putida PaW 95, DSM 5170
12894	P. putida CLB250, DSM 5232
12895	P. putida DSM 84, IMG 1513
12896	P. putida mt-2 KT2440, DSM 6125, ATCC 47054, CCUG 56301, ...
12898	P. putida S-832, DSM 6883
12899	P. putida S-313, DSM 6884
12900	P. putida BG1, DSM 6413
12902	P. putida pUTK9, DSM 6701, RB 1351
12903	P. putida US2, DSM 6755
12904	P. putida F1, PpF1, DSM 6899, ATCC 700007, JCM 31910, ...
12906	P. putida PS, DSM 7189
12907	P. putida Strain G, BKM-BN1123, DSM 7286, BKM BN-1123, ...
12908	P. putida Strain A, DSM 7314, NCIMB 11286, VKM B-1122, ...
12909	P. putida MM1, DSM 9278
12910	P. putida RW20-3b, DSM 11738
12911	P. putida GS1, DSM 12264
12912	P. putida 2-2L, DSM 12585
12913	P. putida PNP1, DSM 12448
12914	P. putida U, DSM 12735, ATCC 17514, NCIMB 10015, CCUG ...
12915	P. putida DSM 13624, IMI 358533
12918	P. putida TM1, DSM 16845
12919	P. putida 50-3-3, IBPM B-20, DSM 46608, CCEB 520, CIP ...
12920	P. putida Berlin 33/2, DSM 50026, CIP 103281
12921	P. putida cl-B, DSM 50198, ATCC 17453, ICPB 2563-77, ...
12922	P. putida 53-2, DSM 50208, ATCC 17485, ICPB 2789-111, ...
12924	P. putida DSM 50257, ATCC 6876, DSM 60118, NCPPB 244, ...
12925	P. putida MT-I1, DSM 21680
12926	P. putida WB, DSM 23849
100159	P. putida ST033293(HKI),
24382	P. putida 160, DSM 50194, ATCC 17529, ICPB 2440-133
24383	P. putida MS of E.E. Snell, DSM 50204, ATCC 17457, ...
24384	P. putida S16, DSM 28022
24385	P. putida B6-2, DSM 28064
130567	P. putida SQ1, DSM 100120
130999	P. putida Z2, DSM 16527
131000	P. putida TEC1, DSM 26234
131001	P. putida TEC1 407.1-delta 2, DSM 26250
131002	P. putida TEC1 407.3-delta2, DSM 26251
131003	P. putida TEC1 407-delta1, DSM 26252
131888	P. putida S13.1.2, DSM 102034
132784	P. putida TEC1 91-delta 1, DSM 26253
134429	P. putida AmMs 106, CIP 104063, ATCC 49128
134979	P. putida CIP 55.5, ATCC 795, IAM 1505, NCDO 754, NCIMB ...
135611	P. putida CIP 59.25, ATCC 12842, NCIMB 8865, JCM 13060, ...
135612	P. putida A3/9, CIP 59.26, NCIMB 8248
135753	P. putida CIP 108916, LMG 1246, ATCC 17430, NCIMB 10534, ...
135983	P. putida CIP 52.195
135984	P. putida CIP 52.196
136153	P. putida CIP 52.193
136154	P. putida CIP 52.197
136155	P. putida CIP 52.198
137196	P. putida CIP 52.194
137217	P. putida CIP 52.190
137776	P. putida 25300, 642-85, CIP 101794
137973	P. putida 340-86, CIP 102341
138643	P. putida 776.85, CIP 101936
138858	P. putida MD 7.3, CIP 59.19, NCIMB 8729
139223	P. putida CIP 52.189
141228	P. putida CCUG 264
141276	P. putida CCUG 553, LMG 2189
141504	P. putida CCUG 2471
141507	P. putida CCUG 2479
141521	P. putida CCUG 2525, NRRL B-2022
142893	P. putida CCUG 15619
143393	P. putida CCUG 18875
144913	P. putida CCUG 27052
145592	P. putida CCUG 28920
145805	P. putida CCUG 29643
146210	P. putida CCUG 30612
146426	P. putida CCUG 31110
146710	P. putida CCUG 32007
147231	P. putida CCUG 33113
147438	P. putida CCUG 33582
147515	P. putida CCUG 33721
147793	P. putida CCUG 34242
148510	P. putida CCUG 35570
149198	P. putida CCUG 37253
150737	P. putida CCUG 42126
153432	P. putida CCUG 49576
153483	P. putida CCUG 49716
153616	P. putida CCUG 50315
153618	P. putida CCUG 50317
154720	P. putida CCUG 54971
155478	P. putida CCUG 57833
156241	P. putida CCUG 60837
156461	P. putida CCUG 61918
161309	P. putida JCM 13062, ATCC 11250, CCM 3656, KCTC 1645, ...
161475	P. putida JCM 14351, ATCC 598, NRRL B-251
162298	P. putida JCM 18798
162685	P. putida JCM 20004, IAM 1002
162686	P. putida JCM 20005, IAM 1003
162700	P. putida JCM 20028, IAM 1049
162701	P. putida JCM 20029, IAM 1050
162713	P. putida JCM 20048, IAM 1094
162723	P. putida JCM 20066, IAM 1128
162741	P. putida JCM 20089, IAM 1177
162752	P. putida JCM 20102, IAM 1200
162760	P. putida JCM 20111, IAM 1218
162761	P. putida JCM 20112, ATCC 17464, IAM 1219
162768	P. putida JCM 20120, IAM 1235
162775	P. putida JCM 20129, IAM 1250
162804	P. putida JCM 20188, ATCC 4359, IAM 1506
162815	P. putida JCM 20228, IAM 1629
165987	P. putida JCM 9045
166071	P. putida JCM 9800
166073	P. putida JCM 9802
174606	P. putida Thor-Nr. 1021, DSM 115323
174607	P. putida 116234, DSM 116234
174608	P. putida 116510, DSM 116510
176535	P. putida NiuAA7, DSM 114486

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
2713	CASO AGAR (MERCK 105458) (DSMZ Medium 220)		Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
2713	MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)		Medium recipe at MediaDive	Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
2713	positive	growth	26
67770	positive	growth	30

Physiology and metabolism

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
2713	-	-	-	+	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	+	+
2713	-	-	-	+	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	+	+

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence LC507958 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	421
Soil Samples	444
Animal Samples	802
Plant Samples	305
Total Samples	1972

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
2713			2	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
124043	ASM3015947v1 assembly for Pseudomonas putida JCM 13061	scaffold	GCA_030159475	303.1155	303	51.5
124043	PDT001638952.1 assembly for Pseudomonas putida	contig	GCA_029749555		303

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Pseudomonas putida strain ATCC 11172 16S ribosomal RNA gene, partial sequence	AF094745	1476	303
20218	Pseudomonas putida 16S ribosomal RNA gene, strain: ATCC 11172, partial sequence	D85992	1329	303
67770	Pseudomonas putida JCM 13061 gene for 16S rRNA, partial sequence	LC507958	1459	303

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Dual-Temperature Microbiological Control of Cellular Products: A Potential Impact for Bacterial Screening of Platelet Concentrates?	Vollmer T, Knabbe C, Dreier J.	Microorganisms	10.3390/microorganisms11092350	2023
Pathogenicity	Probiotic Bifidobacteria Mitigate the Deleterious Effects of para-Cresol in a Drosophila melanogaster Toxicity Model.	Stuivenberg GA, Chmiel JA, Akouris PP, Burton JP, Reid G.	mSphere	10.1128/msphere.00446-22	2022
Biotechnology	Wastewater Treatment Using Membrane Bioreactor Technologies: Removal of Phenolic Contaminants from Oil and Coal Refineries and Pharmaceutical Industries.	Khan MJ, Wibowo A, Karim Z, Posoknistakul P, Matsagar BM, Wu KC, Sakdaronnarong C.	Polymers (Basel)	10.3390/polym16030443	2024
Pathogenicity	Mechanisms of resistance to chloramphenicol in Pseudomonas putida KT2440.	Fernandez M, Conde S, de la Torre J, Molina-Santiago C, Ramos JL, Duque E.	Antimicrob Agents Chemother	10.1128/aac.05398-11	2012
Metabolism	Polymerase chain reaction and gene probe detection of the 2,4-dichlorophenoxyacetic acid degradation plasmid, pJP4.	Neilson JW, Josephson KL, Pillai SD, Pepper IL.	Appl Environ Microbiol	10.1128/aem.58.4.1271-1275.1992	1992
	Bacterial species dominance within a binary culture biofilm.	Banks MK, Bryers JD.	Appl Environ Microbiol	10.1128/aem.57.7.1974-1979.1991	1991
Metabolism	Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates.	Folsom BR, Chapman PJ, Pritchard PH.	Appl Environ Microbiol	10.1128/aem.56.5.1279-1285.1990	1990
	A highly selective PCR protocol for detecting 16S rRNA genes of the genus Pseudomonas (sensu stricto) in environmental samples.	Widmer F, Seidler RJ, Gillevet PM, Watrud LS, Di Giovanni GD.	Appl Environ Microbiol	10.1128/aem.64.7.2545-2553.1998	1998
	Toluene induction and uptake kinetics and their inclusion in the specific-affinity relationship for describing rates of hydrocarbon metabolism.	Robertson BR, Button DK.	Appl Environ Microbiol	10.1128/aem.53.9.2193-2205.1987	1987
	Development of steady-state diffusion gradients for the cultivation of degradative microbial consortia.	Wolfaardt GM, Lawrence JR, Hendry MJ, Robarts RD, Caldwell DE.	Appl Environ Microbiol	10.1128/aem.59.8.2388-2396.1993	1993
Cultivation	A simple diagnostic milk medium for Pseudomonas aeruginosa.	Brown MR, Foster JH.	J Clin Pathol	10.1136/jcp.23.2.172	1970
	Recent advances and trends of trichloroethylene biodegradation: A critical review.	Wu Z, Man Q, Niu H, Lyu H, Song H, Li R, Ren G, Zhu F, Peng C, Li B, Ma X.	Front Microbiol	10.3389/fmicb.2022.1053169	2022
Genetics	Genomic Reclassification and Phenotypic Characterization of Pseudomonas putida Strains Deposited in Japanese Culture Collections.	Morohoshi T, Yaguchi N, Someya N.	Microbes Environ	10.1264/jsme2.me23019	2023
	Isolation and Characterization of Multi-Trait Plant Growth-Promoting Endophytic Bacteria from Scots Pine Tissues.	Shalovylo YI, Yusypovych YM, Kit OY, Kovaleva VA.	J Microbiol Biotechnol	10.4014/jmb.2408.08056	2024
Genetics	Identification of novel non-metal haloperoxidases from the marine metagenome.	Gwon HJ, Teruhiko I, Shigeaki H, Baik SH	J Microbiol Biotechnol	10.4014/jmb.1310.10070	2014
Metabolism	Degradation of phenol and TCE using suspended and chitosan-bead immobilized Pseudomonas putida.	Chen YM, Lin TF, Huang C, Lin JC, Hsieh FM	J Hazard Mater	10.1016/j.jhazmat.2007.03.030	2007
Metabolism	Electrical wiring of Pseudomonas putida and Pseudomonas fluorescens with osmium redox polymers.	Timur S, Haghighi B, Tkac J, Pazarlioglu N, Telefoncu A, Gorton L	Bioelectrochemistry	10.1016/j.bioelechem.2006.08.001	2006
Metabolism	Enhanced biodegradation of phenol by a microbial consortium in a solid-liquid two phase partitioning bioreactor.	Prpich GP, Daugulis AJ	Biodegradation	10.1007/s10532-004-2036-y	2005
Metabolism	Degradation of xenobiotics in a partitioning bioreactor in which the partitioning phase is a polymer.	Amsden BG, Bochanysz J, Daugulis AJ	Biotechnol Bioeng	10.1002/bit.10804	2003
Metabolism	Enhancement of a two-phase partitioning bioreactor system by modification of the microbial catalyst: demonstration of concept.	Vrionis HA, Kropinski AM, Daugulis AJ	Biotechnol Bioeng	10.1002/bit.10313	2002
Enzymology	Expanded application of a two-phase partitioning bioreactor through strain development and new feeding strategies.	Vrionis HA, Kropinski AM, Daugulis AJ	Biotechnol Prog	10.1021/bp020295f	2002
Enzymology	Identification and characterization of the AgmR regulator of Pseudomonas putida: role in alcohol utilization.	Vrionis HA, Daugulis AJ, Kropinski AM	Appl Microbiol Biotechnol	10.1007/s00253-001-0912-5	2002
Metabolism	Biodegradation of phenol at high initial concentrations in two-phase partitioning batch and fed-batch bioreactors.	Collins LD, Daugulis AJ	Biotechnol Bioeng	10.1002/(SICI)1097-0290(19970705)55:1<155::AID-BIT16>3.0.CO;2-L	1997
Metabolism	Characterization and optimization of a two-phase partitioning bioreactor for the biodegradation of phenol.	Collins LD, Daugulis AJ	Appl Microbiol Biotechnol	10.1007/s002530051008	1997
Metabolism	Application of reverse transcriptase PCR for monitoring expression of the catabolic dmpN gene in a phenol-degrading sequencing batch reactor.	Selvaratnam S, Schoedel BA, McFarland BL, Kulpa CF	Appl Environ Microbiol	10.1128/aem.61.11.3981-3985.1995	1995
	Effects of carbon and oxygen limitations and calcium concentrations on biofilm removal processes.	Applegate DH, Bryers JD	Biotechnol Bioeng	10.1002/bit.260370105	1991
Metabolism	Degradation of phenol by Pseudomonas putida ATCC 11172 in continuous culture at different ratios of biofilm surface to culture volume.	Molin G, Nilsson I	Appl Environ Microbiol	10.1128/aem.50.4.946-950.1985	1985
Metabolism	Effect of carbon dioxide on growth of Pseudomonas putida ATCC 11172 on asparagine, citrate, glucose, and lactate in batch and continuous culture.	Molin G	Can J Microbiol	10.1139/m85-143	1985
	Sand administration as an instrument for biofilm control of Pseudomonas putida ATCC 11172 in chemostat cultures.	Molin G, Nilsson I	Biotechnol Bioeng	10.1002/bit.260270114	1985
	Utilization of phenylpropanoids by pseudomonas putida soil isolates and its probable taxonomic significance.	Nonaka K, Ohta H, Sato Y, Hosokawa K	Microbes Environ	10.1264/jsme2.me08545	2008

References

#2713	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6521
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas putida (DSM 6521, ATCC 11172, NCIB 8251)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_030159475

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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