Name: Pseudomonas putida MT53
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12887

Type strain:

Species: Pseudomonas putida

Strain Designation: MT53, pWW53

Culture col. no.: DSM 4303, NCIB 12187

NCBI tax ID(s): 303 (species)

Other strains from Pseudomonas putida

P. putida 90, DSM 291, ATCC 12633, DSM 50202, ... (type strain)
P. putida 1593, DSM 295
P. putida C-1, DSM 437, ATCC 23974, DSM 50253
P. putida 77, DSM 1693, ATCC 8209, CCM 380, ...
P. putida 12/5, DSM 1868
P. putida PaW 340, DSM 2112
P. putida DSM 3226
P. putida mt-2 PaW1, pWWO, DSM 3931, ATCC ...
P. putida MT20, pWW20, DSM 3932, NCIB 12186
P. putida MT15, pWW15, DSM 3936, NCIB 12184
P. putida PaW611, pWW53-4, DSM 3937
P. putida PaW701, pWW60-1, DSM 3938, NCIB ...
P. putida KF439, pKF439, DSM 4011
P. putida PaW736, pWW60-22, DSM 4302, NCIB ...
P. putida CAM, PpG1, DSM 4475
P. putida NAH, PpG7, DSM 4476
P. putida NIC, PpG25, DSM 4477
P. putida PpG2100, SAL, DSM 4478
P. putida PaW 130, DSM 5169
P. putida PaW 95, DSM 5170
P. putida CLB250, DSM 5232
P. putida DSM 84, IMG 1513
P. putida mt-2 KT2440, DSM 6125, ATCC 47054, ...
P. putida S-832, DSM 6883
P. putida S-313, DSM 6884
P. putida BG1, DSM 6413
P. putida DSM 6521, ATCC 11172, NCIB 8251, ...
P. putida pUTK9, DSM 6701, RB 1351
P. putida US2, DSM 6755
P. putida F1, PpF1, DSM 6899, ATCC 700007, ...
P. putida PS, DSM 7189
P. putida Strain G, DSM 7286, BKM BN-1123, ...
P. putida Strain A, DSM 7314, NCIMB 11286, ...
P. putida MM1, DSM 9278
P. putida RW20-3b, DSM 11738
P. putida GS1, DSM 12264
P. putida 2-2L, DSM 12585
P. putida PNP1, DSM 12448
P. putida U, DSM 12735, ATCC 17514, NCIMB ...
P. putida DSM 13624, IMI 358533
P. putida TM1, DSM 16845
P. putida 50-3-3, IBPM B-20, DSM 46608, CCEB ...
P. putida Berlin 33/2, DSM 50026
P. putida cl-B, DSM 50198, ATCC 17453, ICPB ...
P. putida 53-2, DSM 50208, ATCC 17485, ICPB ...
P. putida DSM 50257, ATCC 6876, DSM 60118, ...
P. putida MT-I1, DSM 21680
P. putida WB, DSM 23849
P. putida ST033293(HKI),
P. putida 160, DSM 50194, ATCC 17529, ICPB ...
P. putida MS of E.E. Snell, DSM 50204, ATCC ...
P. putida S16, DSM 28022
P. putida B6-2, DSM 28064
P. putida SQ1, DSM 100120
P. putida Z2, DSM 16527
P. putida TEC1, DSM 26234
P. putida TEC1 407.1-delta 2, DSM 26250
P. putida TEC1 407.3-delta2, DSM 26251
P. putida TEC1 407-delta1, DSM 26252
P. putida S13.1.2, DSM 102034
P. putida TEC1 91-delta 1, DSM 26253
P. putida CIP 104063, ATCC 49128
P. putida CIP 55.5, ATCC 795, IAM 1505, NCDO ...
P. putida CIP 59.25, ATCC 12842, NCIMB 8865, ...
P. putida CIP 59.26, NCIMB 8248
P. putida CIP 108916, LMG 1246, ATCC 17430, ...
P. putida CIP 52.195
P. putida CIP 52.196
P. putida CIP 52.193
P. putida CIP 52.197
P. putida CIP 52.198
P. putida CIP 52.194
P. putida CIP 52.190
P. putida CIP 101794
P. putida CIP 102341
P. putida CIP 101936
P. putida CIP 59.19, NCIMB 8729
P. putida CIP 52.189
P. putida CCUG 264
P. putida CCUG 553, LMG 2189
P. putida CCUG 2471
P. putida CCUG 2479
P. putida CCUG 2525, NRRL B-2022
P. putida CCUG 15619
P. putida CCUG 18875
P. putida CCUG 27052
P. putida CCUG 28920
P. putida CCUG 29643
P. putida CCUG 30612
P. putida CCUG 31110
P. putida CCUG 32007
P. putida CCUG 33113
P. putida CCUG 33582
P. putida CCUG 33721
P. putida CCUG 34242
P. putida CCUG 35570
P. putida CCUG 37253
P. putida CCUG 42126
P. putida CCUG 49576
P. putida CCUG 49716
P. putida CCUG 50315
P. putida CCUG 50317
P. putida CCUG 54971
P. putida CCUG 57833
P. putida CCUG 60837
P. putida CCUG 61918
P. putida JCM 13062, ATCC 11250, CCM 3656, ...
P. putida JCM 14351, ATCC 598, NRRL B-251
P. putida JCM 18798
P. putida JCM 20004, IAM 1002
P. putida JCM 20005, IAM 1003
P. putida JCM 20028, IAM 1049
P. putida JCM 20029, IAM 1050
P. putida JCM 20048, IAM 1094
P. putida JCM 20066, IAM 1128
P. putida JCM 20089, IAM 1177
P. putida JCM 20102, IAM 1200
P. putida JCM 20111, IAM 1218
P. putida JCM 20112, ATCC 17464, IAM 1219
P. putida JCM 20120, IAM 1235
P. putida JCM 20129, IAM 1250
P. putida JCM 20188, ATCC 4359, IAM 1506
P. putida JCM 20228, IAM 1629
P. putida JCM 9045
P. putida JCM 9800
P. putida JCM 9802

Pseudomonas putida MT53 is a bacterium of the family Pseudomonadaceae.

Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (PNU)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #1647]	21741	-	-	-	-	-	-	-	-	+	+	+	-	-	-	+	+	-	+	+	+	+

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1647]

Biosafety level

Risk group (German classification)
According to TRBA 466

Availability in culture collections External links

[Ref.: #1647]

Culture collection no.

DSM 4303, NCIB 12187

[Ref.: #20218]

Associated Passport(s) in StrainInfo

339248

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid compositions and immunological cross-reactions.	Chalmers RM, Keen JN, Fewson CA	Biochem J	10.1042/bj2730099	1991	*
Enzymology	Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53.	Keil H, Keil S, Pickup RW, Williams PA	J Bacteriol	10.1128/jb.164.2.887-895.1985	1985	*
Enzymology	Gene organization of the first catabolic operon of TOL plasmid pWW53: production of indigo by the xylA gene product.	Keil H, Saint CM, Williams PA	J Bacteriol	10.1128/jb.169.2.764-770.1987	1987	*
Metabolism	Physical and functional mapping of two cointegrate plasmids derived from RP4 and TOL plasmid pDK1.	Shaw LE, Williams PA	J Gen Microbiol	10.1099/00221287-134-9-2463	1988	*
Enzymology	Molecular analysis of regulatory and structural xyl genes of the TOL plasmid pWW53-4.	Keil H, Keil S, Williams PA	J Gen Microbiol	10.1099/00221287-133-5-1149	1987	*
Metabolism	Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53.	Gallegos MT, Williams PA, Ramos JL	J Bacteriol	10.1128/jb.179.16.5024-5029.1997	1997	*
Enzymology	Implications of the xylQ gene of TOL plasmid pWW102 for the evolution of aromatic catabolic pathways.	Aemprapa S, Williams PA	Microbiology (Reading)	10.1099/00221287-144-5-1387	1998	*
Metabolism	Aromatic hydrocarbon degradation patterns and catechol 2,3-dioxygenase genes in microbial cultures from deep anoxic hypersaline lakes in the eastern Mediterranean sea.	Brusa T, Borin S, Ferrari F, Sorlini C, Corselli C, Daffonchio D	Microbiol Res	10.1078/0944-5013-00075	2001	*
Genetics	Identification and characterization of Tn4656, a novel class II transposon carrying a set of toluene-degrading genes from TOL plasmid pWW53.	Tsuda M, Genka H	J Bacteriol	10.1128/JB.183.21.6215-6224.2001	2001	*
Genetics	Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements.	Yano H, Garruto CE, Sota M, Ohtsubo Y, Nagata Y, Zylstra GJ, Williams PA, Tsuda M	J Mol Biol	10.1016/j.jmb.2007.02.098	2007	*
Genetics	Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids.	Yano H, Miyakoshi M, Ohshima K, Tabata M, Nagata Y, Hattori M, Tsuda M	J Bacteriol	10.1128/JB.00359-10	2010	*
Metabolism	ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island, interferes with the partition system of IncP-7 plasmids.	Miyakoshi M, Shintani M, Inoue K, Terabayashi T, Sai F, Ohkuma M, Nojiri H, Nagata Y, Tsuda M	Environ Microbiol	10.1111/j.1462-2920.2012.02861.x	2012	*
Metabolism	Complete nucleotide sequence of the self-transmissible TOL plasmid pD2RT provides new insight into arrangement of toluene catabolic plasmids.	Jutkina J, Hansen LH, Li L, Heinaru E, Vedler E, Joesaar M, Heinaru A	Plasmid	10.1016/j.plasmid.2013.09.003	2013	*

References

#1647

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4303

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#68369

Automatically annotated from API 20NE .

* These data were automatically processed and therefore are not curated

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