Name: Pseudomonas putida MT53
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12887

Type strain:

Species: Pseudomonas putida

Strain Designation: MT53, pWW53

Culture col. no.: DSM 4303, NCIB 12187

NCBI tax ID(s): 303 (species)

SI-ID 112668

DSM 4303

Special Collections

DSMZ

Other strains from Pseudomonas putida

Pseudomonas putida MT53 is a bacterium of the family Pseudomonadaceae.

Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (LPSN)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #1647]	24349	-	-	-	-	-	-	-	-	+	+	+	-	-	-	+	+	-	+	+	+	+

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1647]

Biosafety level

Risk group (German classification)
According to TRBA 466

Availability in culture collections External links

[Ref.: #1647]

Culture collection no.

DSM 4303, NCIB 12187

[Ref.: #82100]

SI-ID 112668

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Complete nucleotide sequence of the self-transmissible TOL plasmid pD2RT provides new insight into arrangement of toluene catabolic plasmids.	Jutkina J, Hansen LH, Li L, Heinaru E, Vedler E, Joesaar M, Heinaru A	Plasmid	10.1016/j.plasmid.2013.09.003	2013	*
Metabolism	ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island, interferes with the partition system of IncP-7 plasmids.	Miyakoshi M, Shintani M, Inoue K, Terabayashi T, Sai F, Ohkuma M, Nojiri H, Nagata Y, Tsuda M	Environ Microbiol	10.1111/j.1462-2920.2012.02861.x	2012	*
Genetics	Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids.	Yano H, Miyakoshi M, Ohshima K, Tabata M, Nagata Y, Hattori M, Tsuda M	J Bacteriol	10.1128/JB.00359-10	2010	*
Genetics	Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements.	Yano H, Garruto CE, Sota M, Ohtsubo Y, Nagata Y, Zylstra GJ, Williams PA, Tsuda M	J Mol Biol	10.1016/j.jmb.2007.02.098	2007	*
Metabolism	Aromatic hydrocarbon degradation patterns and catechol 2,3-dioxygenase genes in microbial cultures from deep anoxic hypersaline lakes in the eastern Mediterranean sea.	Brusa T, Borin S, Ferrari F, Sorlini C, Corselli C, Daffonchio D	Microbiol Res	10.1078/0944-5013-00075	2001	*
Enzymology	Implications of the xylQ gene of TOL plasmid pWW102 for the evolution of aromatic catabolic pathways.	Aemprapa S, Williams PA	Microbiology (Reading)	10.1099/00221287-144-5-1387	1998	*
Metabolism	Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53.	Gallegos MT, Williams PA, Ramos JL	J Bacteriol	10.1128/jb.179.16.5024-5029.1997	1997	*
Enzymology	Comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid compositions and immunological cross-reactions.	Chalmers RM, Keen JN, Fewson CA	Biochem J	10.1042/bj2730099	1991	*
Enzymology	Gene organization of the first catabolic operon of TOL plasmid pWW53: production of indigo by the xylA gene product.	Keil H, Saint CM, Williams PA	J Bacteriol	10.1128/jb.169.2.764-770.1987	1987	*
Genetics	Identification and characterization of Tn4656, a novel class II transposon carrying a set of toluene-degrading genes from TOL plasmid pWW53.	Tsuda M, Genka H	J Bacteriol	10.1128/JB.183.21.6215-6224.2001	2001	*
Metabolism	Physical and functional mapping of two cointegrate plasmids derived from RP4 and TOL plasmid pDK1.	Shaw LE, Williams PA	J Gen Microbiol	10.1099/00221287-134-9-2463	1988	*
Enzymology	Molecular analysis of regulatory and structural xyl genes of the TOL plasmid pWW53-4.	Keil H, Keil S, Williams PA	J Gen Microbiol	10.1099/00221287-133-5-1149	1987	*
Enzymology	Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53.	Keil H, Keil S, Pickup RW, Williams PA	J Bacteriol	10.1128/jb.164.2.887-895.1985	1985	*

References

#1647

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4303

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#68369

Automatically annotated from API 20NE .

#82100

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID112668.1 )

* These data were automatically processed and therefore are not curated

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