Strain identifier
BacDive ID: 12887
Type strain: 
Species: Pseudomonas putida
Strain Designation: MT53, pWW53
NCBI tax ID(s): 303 (species)
version 8.1 (current version)
General
@ref: 1647
BacDive-ID: 12887
DSM-Number: 4303
keywords: Bacteria
description: Pseudomonas putida MT53 is a bacterium of the family Pseudomonadaceae.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
doi: 10.13145/bacdive12887.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 1647
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Pseudomonadales
family: Micrococcaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: MT53, pWW53
type strain: no
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1647 | - | - | - | - | - | - | - | - | + | + | + | - | - | - | + | + | - | + | + | + | + |
Safety information
risk assessment
- @ref: 1647
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 1647
culture collection no.: DSM 4303, NCIB 12187
straininfo link
- @ref: 82100
- straininfo: 112668
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 1989592 | Comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid compositions and immunological cross-reactions. | Chalmers RM, Keen JN, Fewson CA | Biochem J | 10.1042/bj2730099 | 1991 | Acinetobacter/*enzymology, Alcohol Oxidoreductases/*chemistry/immunology, Aldehyde Oxidoreductases/*chemistry/immunology, Amino Acid Sequence, Amino Acids/analysis, Benzaldehyde Dehydrogenase (NADP+), Benzyl Alcohol, Benzyl Alcohols/*metabolism, Blotting, Western, Cross Reactions, Genes, Bacterial, Immune Sera, Mandelic Acids/*metabolism, Molecular Sequence Data, Plasmids, Precipitin Tests, Pseudomonas/*enzymology/genetics, Toluene/*metabolism | Metabolism |
| Enzymology | 2997136 | Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53. | Keil H, Keil S, Pickup RW, Williams PA | J Bacteriol | 10.1128/jb.164.2.887-895.1985 | 1985 | Alcohol Oxidoreductases/biosynthesis/genetics, *Aldehyde Oxidoreductases, *Benzoates/*metabolism, Biological Evolution, Catechol 2,3-Dioxygenase, Cloning, Molecular, DNA Restriction Enzymes, DNA, Recombinant, *Dioxygenases, Enzyme Induction, Genes, Regulator, Hydrolases/biosynthesis/genetics, Operon, Oxygenases/biosynthesis/genetics, *Plasmids, *Proteins, Pseudomonas/enzymology/*genetics/metabolism, Xylenes/*metabolism | Metabolism |
| Enzymology | 3027047 | Gene organization of the first catabolic operon of TOL plasmid pWW53: production of indigo by the xylA gene product. | Keil H, Saint CM, Williams PA | J Bacteriol | 10.1128/jb.169.2.764-770.1987 | 1987 | Bacterial Proteins/*genetics, Cloning, Molecular, Escherichia coli/genetics, *Genes, Bacterial, Indigo Carmine, Indoles/*metabolism, Mutation, Nucleic Acid Hybridization, *Operon, *Plasmids, Promoter Regions, Genetic, Pseudomonas/*genetics/metabolism | Metabolism |
| Metabolism | 3076182 | Physical and functional mapping of two cointegrate plasmids derived from RP4 and TOL plasmid pDK1. | Shaw LE, Williams PA | J Gen Microbiol | 10.1099/00221287-134-9-2463 | 1988 | Chromosome Mapping, DNA, Recombinant, Escherichia coli/genetics, Genes, Bacterial, Genes, Regulator, Operon, *Plasmids, Pseudomonas/*genetics, R Factors/*genetics, Restriction Mapping, Toluene/metabolism, Xylenes/metabolism | |
| Enzymology | 3309179 | Molecular analysis of regulatory and structural xyl genes of the TOL plasmid pWW53-4. | Keil H, Keil S, Williams PA | J Gen Microbiol | 10.1099/00221287-133-5-1149 | 1987 | Chromosome Mapping, DNA, Bacterial, Enzyme Induction, Escherichia coli/enzymology, *Genes, *Genes, Bacterial, *Genes, Regulator, Promoter Regions, Genetic, Pseudomonas/enzymology/genetics, Toluene/*metabolism | Metabolism |
| Metabolism | 9260942 | Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53. | Gallegos MT, Williams PA, Ramos JL | J Bacteriol | 10.1128/jb.179.16.5024-5029.1997 | 1997 | *Bacterial Proteins, Base Sequence, Benzoates/metabolism, Benzyl Alcohols/metabolism, Culture Media, DNA-Binding Proteins/*genetics/metabolism, Gene Expression Regulation, Bacterial, Genes, Regulator, Molecular Sequence Data, *Operon, *Plasmids, Promoter Regions, Genetic, Pseudomonas putida/*genetics/metabolism, RNA, Bacterial/genetics/metabolism, RNA, Messenger/genetics/metabolism, Toluene/metabolism, Trans-Activators/*genetics/metabolism, Transcription Factors/*genetics/metabolism, *Transcription, Genetic, Xylenes/metabolism | Cultivation |
| Enzymology | 9611813 | Implications of the xylQ gene of TOL plasmid pWW102 for the evolution of aromatic catabolic pathways. | Aemprapa S, Williams PA | Microbiology (Reading) | 10.1099/00221287-144-5-1387 | 1998 | Aldehyde Oxidoreductases/*genetics/metabolism, Amino Acid Sequence, Base Sequence, Blotting, Southern, Cloning, Molecular, DNA, Bacterial, Gene Expression Regulation, Bacterial, Genes, Bacterial, Genes, Regulator, Genetic Linkage, Molecular Sequence Data, *Operon, *Plasmids, Pseudomonas putida/enzymology/*genetics/growth & development/*metabolism, Restriction Mapping, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Structure-Activity Relationship, Toluene/metabolism, Xylenes/*metabolism | Metabolism |
| Metabolism | 11372653 | Aromatic hydrocarbon degradation patterns and catechol 2,3-dioxygenase genes in microbial cultures from deep anoxic hypersaline lakes in the eastern Mediterranean sea. | Brusa T, Borin S, Ferrari F, Sorlini C, Corselli C, Daffonchio D | Microbiol Res | 10.1078/0944-5013-00075 | 2001 | Catechol 2,3-Dioxygenase, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics/isolation & purification, DNA, Ribosomal/chemistry/genetics/isolation & purification, *Dioxygenases, Genes, Bacterial, Genetic Variation, Hydrocarbons, Aromatic/*metabolism, Mediterranean Sea, Oxygenases/chemistry/*genetics/metabolism, Phylogeny, Polymerase Chain Reaction, Polymorphism, Single-Stranded Conformational, Pseudomonas/enzymology/*genetics/*metabolism, Seawater/*microbiology, Sequence Alignment, Sequence Analysis, DNA | Phylogeny |
| Genetics | 11591664 | Identification and characterization of Tn4656, a novel class II transposon carrying a set of toluene-degrading genes from TOL plasmid pWW53. | Tsuda M, Genka H | J Bacteriol | 10.1128/JB.183.21.6215-6224.2001 | 2001 | *Bacterial Proteins, DNA Transposable Elements/*genetics, DNA-Binding Proteins/*genetics, *Genes, Bacterial, Genetic Complementation Test, Molecular Sequence Data, *Plasmids, Pseudomonas putida/genetics/metabolism, Sequence Homology, Nucleic Acid, Toluene/*metabolism, Trans-Activators/*genetics, Transcription Factors/*genetics | Metabolism |
| Genetics | 17408691 | Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements. | Yano H, Garruto CE, Sota M, Ohtsubo Y, Nagata Y, Zylstra GJ, Williams PA, Tsuda M | J Mol Biol | 10.1016/j.jmb.2007.02.098 | 2007 | Base Sequence, DNA Transposable Elements, DNA, Bacterial/genetics, Genes, Bacterial, Interspersed Repetitive Sequences/*genetics, Molecular Sequence Data, Phylogeny, Plasmids/*genetics, Pseudomonas putida/*genetics, Recombination, Genetic/*genetics, *Sequence Analysis, DNA, Substrate Specificity, Toluene/*metabolism, Transposases/metabolism, Transposon Resolvases/metabolism | Metabolism |
| Genetics | 20581207 | Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids. | Yano H, Miyakoshi M, Ohshima K, Tabata M, Nagata Y, Hattori M, Tsuda M | J Bacteriol | 10.1128/JB.00359-10 | 2010 | Bacterial Proteins/genetics, Conjugation, Genetic, DNA Transposable Elements/genetics, *Evolution, Molecular, Molecular Sequence Data, Multigene Family, Plasmids/*genetics, Pseudomonas putida/*genetics/metabolism, Recombination, Genetic, Sequence Analysis, DNA, Toluene/*metabolism | Metabolism |
| Metabolism | 22925377 | ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island, interferes with the partition system of IncP-7 plasmids. | Miyakoshi M, Shintani M, Inoue K, Terabayashi T, Sai F, Ohkuma M, Nojiri H, Nagata Y, Tsuda M | Environ Microbiol | 10.1111/j.1462-2920.2012.02861.x | 2012 | Bacterial Proteins/*genetics/*metabolism, DNA Replication, Genomic Islands/*genetics, Plasmids/*genetics, Pseudomonas putida/*genetics/*metabolism | Genetics |
| Metabolism | 24095800 | Complete nucleotide sequence of the self-transmissible TOL plasmid pD2RT provides new insight into arrangement of toluene catabolic plasmids. | Jutkina J, Hansen LH, Li L, Heinaru E, Vedler E, Joesaar M, Heinaru A | Plasmid | 10.1016/j.plasmid.2013.09.003 | 2013 | Aquatic Organisms/*genetics/growth & development/metabolism, Base Sequence, Biodegradation, Environmental, Burkholderia/genetics, DNA Transposable Elements, Molecular Sequence Data, *Open Reading Frames, *Plasmids, Pseudomonas/*genetics/growth & development/metabolism, Pseudomonas putida/genetics, Pseudomonas syringae/genetics, Sequence Alignment, Sequence Homology, Nucleic Acid, Toluene/*metabolism, Water Pollutants, Chemical/*metabolism | Genetics |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 1647 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4303) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4303 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 82100 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID112668.1 | StrainInfo: A central database for resolving microbial strain identifiers |