Name: Pseudomonas putida mt-2 KT2440
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12896

Type strain:

Species: Pseudomonas putida

Strain Designation: mt-2 KT2440

Culture col. no.: DSM 6125, ATCC 47054, CCUG 56301, CFBP 8728

Strain history: <- CSIC <- K.N. Timmis

NCBI tax ID(s): 303 (species)

SI-ID 42206

ATCC 47054, DSM 6125, NCIMB 11950

Special Collections

DSMZ
CCUG

Other strains from Pseudomonas putida

Section

Pseudomonas putida mt-2 KT2440 is a bacterium that was isolated from Safety host for recombinant DNA experiments.

Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (LPSN)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2385]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2385]

Culture medium growth

[Ref.: #2385]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2385]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #2385]			growth	28 °C

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2385]	24373	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+
[Ref.: #2385]	24372	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #60908]	Sample type/isolated from	Safety host for recombinant DNA experiments(r-,m+)
* marker position based on {}

Isolation sources categories

#Engineered

#Laboratory

What are isolation sources categories?

Availability in culture collections External links

[Ref.: #2385]

Culture collection no.

DSM 6125, ATCC 47054, CCUG 56301, CFBP 8728

[Ref.: #82109]

SI-ID 42206

Literature:

	Title	Authors	Journal	DOI	Year
Metabolism	Valorization of Gelidium amansii for dual production of D-galactonic acid and 5-hydroxymethyl-2-furancarboxylic acid by chemo-biological approach.	Liu P, Xie J, Tan H, Zhou F, Zou L, Ouyang J	Microb Cell Fact	10.1186/s12934-020-01357-6	2020	*
Enzymology	A process for production of trehalose by recombinant trehalose synthase and its purification.	Liu H, Yang S, Liu Q, Wang R, Wang T	Enzyme Microb Technol	10.1016/j.enzmictec.2017.11.008	2017	*
Metabolism	Biodegradation of Low Density Polythene (LDPE) by Pseudomonas Species.	Kyaw BM, Champakalakshmi R, Sakharkar MK, Lim CS, Sakharkar KR	Indian J Microbiol	10.1007/s12088-012-0250-6	2012	*
	Evaluation of 3D-Printing Scaffold Fabrication on Biosynthetic Medium-Chain-Length Polyhydroxyalkanoate Terpolyester as Biomaterial-Ink.	Panaksri A, Tanadchangsaeng N	Polymers (Basel)	10.3390/polym13142222	2021	*
	Cell-Surface Displayed Expression of Trehalose Synthase from Pseudomonas putida ATCC 47054 in Pichia Pastoris Using Pir1p as an Anchor Protein.	Yang S, Lv X, Wang X, Wang J, Wang R, Wang T	Front Microbiol	10.3389/fmicb.2017.02583	2017	*

References

#2385

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6125

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#60908 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56301

#68369

Automatically annotated from API 20NE .

#82109

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42206.1 )

* These data were automatically processed and therefore are not curated

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