Name: Pseudomonas putida mt-2 KT2440
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12896

Type strain:

Species: Pseudomonas putida

Strain Designation: mt-2 KT2440

Culture col. no.: DSM 6125, ATCC 47054, CCUG 56301, CFBP 8728

Strain history: <- CSIC <- K.N. Timmis

NCBI tax ID(s): 303 (species)

SI-ID 42206

ATCC 47054, DSM 6125, NCIMB 11950

Other strains from Pseudomonas putida

P. putida 90, A 3.12, DSM 291, ATCC 12633, ... (type strain)
P. putida 1593, DSM 295
P. putida C-1, DSM 437, ATCC 23974, DSM 50253
P. putida 77, DSM 1693, ATCC 8209, CCM 380, ...
P. putida 12/5, DSM 1868
P. putida PaW 340, DSM 2112
P. putida DSM 3226
P. putida mt-2 PaW1, pWWO, DSM 3931, ATCC ...
P. putida MT20, pWW20, DSM 3932, NCIB 12186
P. putida MT15, pWW15, DSM 3936, NCIB 12184
P. putida PaW611, pWW53-4, DSM 3937
P. putida PaW701, pWW60-1, DSM 3938, NCIB ...
P. putida KF439, pKF439, DSM 4011
P. putida PaW736, pWW60-22, DSM 4302, NCIB ...
P. putida MT53, pWW53, DSM 4303, NCIB 12187
P. putida CAM, PpG1, DSM 4475
P. putida NAH, PpG7, DSM 4476
P. putida NIC, PpG25, DSM 4477
P. putida PpG2100, SAL, DSM 4478
P. putida PaW 130, DSM 5169
P. putida PaW 95, DSM 5170
P. putida CLB250, DSM 5232
P. putida DSM 84, IMG 1513
P. putida S-832, DSM 6883
P. putida S-313, DSM 6884
P. putida BG1, DSM 6413
P. putida DSM 6521, ATCC 11172, NCIB 8251, ...
P. putida pUTK9, DSM 6701, RB 1351
P. putida US2, DSM 6755
P. putida F1, PpF1, DSM 6899, ATCC 700007, ...
P. putida PS, DSM 7189
P. putida Strain G, DSM 7286, BKM BN-1123, ...
P. putida Strain A, DSM 7314, NCIMB 11286, ...
P. putida MM1, DSM 9278
P. putida RW20-3b, DSM 11738
P. putida GS1, DSM 12264
P. putida 2-2L, DSM 12585
P. putida PNP1, DSM 12448
P. putida U, DSM 12735, ATCC 17514, NCIMB ...
P. putida DSM 13624, IMI 358533
P. putida TM1, DSM 16845
P. putida 50-3-3, IBPM B-20, DSM 46608, CCEB ...
P. putida Berlin 33/2, DSM 50026, CIP 103281
P. putida cl-B, DSM 50198, ATCC 17453, ICPB ...
P. putida 53-2, DSM 50208, ATCC 17485, ICPB ...
P. putida DSM 50257, ATCC 6876, DSM 60118, ...
P. putida MT-I1, DSM 21680
P. putida WB, DSM 23849
P. putida ST033293(HKI),
P. putida 160, DSM 50194, ATCC 17529, ICPB ...
P. putida MS of E.E. Snell, DSM 50204, ATCC ...
P. putida S16, DSM 28022
P. putida B6-2, DSM 28064
P. putida SQ1, DSM 100120
P. putida Z2, DSM 16527
P. putida TEC1, DSM 26234
P. putida TEC1 407.1-delta 2, DSM 26250
P. putida TEC1 407.3-delta2, DSM 26251
P. putida TEC1 407-delta1, DSM 26252
P. putida S13.1.2, DSM 102034
P. putida TEC1 91-delta 1, DSM 26253
P. putida AmMs 106, CIP 104063, ATCC 49128
P. putida CIP 55.5, ATCC 795, IAM 1505, NCDO ...
P. putida CIP 59.25, ATCC 12842, NCIMB 8865, ...
P. putida A3/9, CIP 59.26, NCIMB 8248
P. putida CIP 108916, LMG 1246, ATCC 17430, ...
P. putida CIP 52.195
P. putida CIP 52.196
P. putida CIP 52.193
P. putida CIP 52.197
P. putida CIP 52.198
P. putida CIP 52.194
P. putida CIP 52.190
P. putida 25300, 642-85, CIP 101794
P. putida 340-86, CIP 102341
P. putida 776.85, CIP 101936
P. putida MD 7.3, CIP 59.19, NCIMB 8729
P. putida CIP 52.189
P. putida CCUG 264
P. putida CCUG 553, LMG 2189
P. putida CCUG 2471
P. putida CCUG 2479
P. putida CCUG 2525, NRRL B-2022
P. putida CCUG 15619
P. putida CCUG 18875
P. putida CCUG 27052
P. putida CCUG 28920
P. putida CCUG 29643
P. putida CCUG 30612
P. putida CCUG 31110
P. putida CCUG 32007
P. putida CCUG 33113
P. putida CCUG 33582
P. putida CCUG 33721
P. putida CCUG 34242
P. putida CCUG 35570
P. putida CCUG 37253
P. putida CCUG 42126
P. putida CCUG 49576
P. putida CCUG 49716
P. putida CCUG 50315
P. putida CCUG 50317
P. putida CCUG 54971
P. putida CCUG 57833
P. putida CCUG 60837
P. putida CCUG 61918
P. putida JCM 13062, ATCC 11250, CCM 3656, ...
P. putida JCM 14351, ATCC 598, NRRL B-251
P. putida JCM 18798
P. putida JCM 20004, IAM 1002
P. putida JCM 20005, IAM 1003
P. putida JCM 20028, IAM 1049
P. putida JCM 20029, IAM 1050
P. putida JCM 20048, IAM 1094
P. putida JCM 20066, IAM 1128
P. putida JCM 20089, IAM 1177
P. putida JCM 20102, IAM 1200
P. putida JCM 20111, IAM 1218
P. putida JCM 20112, ATCC 17464, IAM 1219
P. putida JCM 20120, IAM 1235
P. putida JCM 20129, IAM 1250
P. putida JCM 20188, ATCC 4359, IAM 1506
P. putida JCM 20228, IAM 1629
P. putida JCM 9045
P. putida JCM 9800
P. putida JCM 9802

Section

Pseudomonas putida mt-2 KT2440 is a mesophilic bacterium that was isolated from Safety host for recombinant DNA experiments.

mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (LPSN)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2385]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2385]

Culture medium growth

[Ref.: #2385]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2385]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #2385]			growth	28 °C

[Ref.: #2385]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2385]	24373	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+
[Ref.: #2385]	24372	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #60908]	Sample type/isolated from	Safety host for recombinant DNA experiments(r-,m+)
* marker position based on {}

Isolation sources categories

#Engineered

#Laboratory

What are isolation sources categories?

Availability in culture collections External links

[Ref.: #2385]

Culture collection no.

DSM 6125, ATCC 47054, CCUG 56301, CFBP 8728

[Ref.: #82109]

SI-ID 42206

Literature:

	Title	Authors	Journal	DOI	Year
Metabolism	Valorization of Gelidium amansii for dual production of D-galactonic acid and 5-hydroxymethyl-2-furancarboxylic acid by chemo-biological approach.	Liu P, Xie J, Tan H, Zhou F, Zou L, Ouyang J	Microb Cell Fact	10.1186/s12934-020-01357-6	2020	*
Enzymology	A process for production of trehalose by recombinant trehalose synthase and its purification.	Liu H, Yang S, Liu Q, Wang R, Wang T	Enzyme Microb Technol	10.1016/j.enzmictec.2017.11.008	2017	*
Metabolism	Biodegradation of Low Density Polythene (LDPE) by Pseudomonas Species.	Kyaw BM, Champakalakshmi R, Sakharkar MK, Lim CS, Sakharkar KR	Indian J Microbiol	10.1007/s12088-012-0250-6	2012	*
	Evaluation of 3D-Printing Scaffold Fabrication on Biosynthetic Medium-Chain-Length Polyhydroxyalkanoate Terpolyester as Biomaterial-Ink.	Panaksri A, Tanadchangsaeng N	Polymers (Basel)	10.3390/polym13142222	2021	*
	Cell-Surface Displayed Expression of Trehalose Synthase from Pseudomonas putida ATCC 47054 in Pichia Pastoris Using Pir1p as an Anchor Protein.	Yang S, Lv X, Wang X, Wang J, Wang R, Wang T	Front Microbiol	10.3389/fmicb.2017.02583	2017	*

References

#2385

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6125

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#60908 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56301

#68369

Automatically annotated from API 20NE .

#82109

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42206.1 )

* These data were automatically processed and therefore are not curated

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