Pseudomonas putida mt-2 KT2440 is a mesophilic prokaryote that was isolated from Safety host for recombinant DNA experiments.
mesophilic genome sequence| @ref 20215 |
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Last LPSN update
2025-12-16 09:49:57 |
| Domain Pseudomonadati |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Pseudomonadales |
| Family Pseudomonadaceae |
| Genus Pseudomonas |
| Species Pseudomonas putida |
| Full scientific name Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980) |
| Synonyms (2) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 2385 | NUTRIENT AGAR (DSMZ Medium 1) | Medium recipe at MediaDive  | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 2385 | positive | growth | 28 | mesophilic |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68369 | 17128 ChEBI | adipate | - | assimilation | from API 20NE |
| 68369 | 29016 ChEBI | arginine | + | hydrolysis | from API 20NE |
| 68369 | 17634 ChEBI | D-glucose | + | assimilation | from API 20NE |
| 68369 | 17634 ChEBI | D-glucose | - | fermentation | from API 20NE |
| 68369 | 16899 ChEBI | D-mannitol | - | assimilation | from API 20NE |
| 68369 | 16024 ChEBI | D-mannose | + | assimilation | from API 20NE |
| 68369 | 27689 ChEBI | decanoate | + | assimilation | from API 20NE |
| 68369 | 4853 ChEBI | esculin | - | hydrolysis | from API 20NE |
| 68369 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20NE |
| 68369 | 24265 ChEBI | gluconate | + | assimilation | from API 20NE |
| 68369 | 30849 ChEBI | L-arabinose | - | assimilation | from API 20NE |
| 68369 | 25115 ChEBI | malate | + | assimilation | from API 20NE |
| 68369 | 17306 ChEBI | maltose | - | assimilation | from API 20NE |
| 68369 | 59640 ChEBI | N-acetylglucosamine | - | assimilation | from API 20NE |
| 68369 | 17632 ChEBI | nitrate | - | reduction | from API 20NE |
| 68369 | 27897 ChEBI | tryptophan | - | energy source | from API 20NE |
| 68369 | 16199 ChEBI | urea | - | hydrolysis | from API 20NE |
| @ref | Description | Assembly level | INSDC accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|
| 124043 | Engineered_P.putida_DSM6125 assembly for Pseudomonas putida | complete | GCA_038095575   | 303 | 98.93 | |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Enzymology | Analysis of Benzoate 1,2-Dioxygenase Identifies Shared Electron Transfer Components With DxnA1A2 in Rhizorhabdus wittichii RW1. | Ivanovski I, Eleya S, Zylstra GJ. | J Basic Microbiol | 10.1002/jobm.70061 | 2025 | |
| Effect of microplastic on sorption, toxicity, and mineralization of 2,4-dichlorophenoxyacetic acid ionic liquids. | Lisiecka N, Wozniak-Karczewska M, Parus A, Simpson M, Frankowski R, Zgola-Grzeskowiak A, Siwinska-Ciesielczyk K, Niemczak M, Eberlein C, Heipieper HJ, Chrzanowski L. | Appl Microbiol Biotechnol | 10.1007/s00253-024-13353-6 | 2024 | | |
| Conversion and upgrading of syringate by Acinetobacter baylyi ADP1. | Tuomela H, Koivisto J, Efimova E, Santala S. | Microb Cell Fact | 10.1186/s12934-025-02839-1 | 2025 | | |
| Genetics | Unraveling the genomic diversity of the Pseudomonas putida group: exploring taxonomy, core pangenome, and antibiotic resistance mechanisms. | Udaondo Z, Ramos JL, Abram K. | FEMS Microbiol Rev | 10.1093/femsre/fuae025 | 2024 | |
| Revealing taxonomy, activity, and substrate assimilation in mixed bacterial communities by GroEL-proteotyping-based stable isotope probing. | Klaes S, Madan S, Deobald D, Cooper M, Adrian L. | iScience | 10.1016/j.isci.2024.111249 | 2024 | | |
| Physiology, fast and slow: bacterial response to variable resource stoichiometry and dilution rate. | Peoples LM, Isanta-Navarro J, Bras B, Hand BK, Rosenzweig F, Elser JJ, Church MJ. | mSystems | 10.1128/msystems.00770-24 | 2024 | | |
| Metabolism | Systems biology of electrogenic Pseudomonas putida - multi-omics insights and metabolic engineering for enhanced 2-ketogluconate production. | Weimer A, Pause L, Ries F, Kohlstedt M, Adrian L, Kromer J, Lai B, Wittmann C. | Microb Cell Fact | 10.1186/s12934-024-02509-8 | 2024 | |
| Enzymology | Seamless and orthogonal expression of genetic parts in polyhydroxyalkanoate (PHA)-producing bacterial chassis for plastic bio-upcycling applications. | Minggu MM, Naseron NAH, Shaberi HSA, Muhammad NAN, Baharum SN, Ramzi AB. | MethodsX | 10.1016/j.mex.2023.102434 | 2023 | |
| From degrader to producer: reversing the gallic acid metabolism of Pseudomonas putida KT2440. | Dias FMS, Pantoja RK, Gomez JGC, Silva LF. | Int Microbiol | 10.1007/s10123-022-00282-5 | 2023 | | |
| An efficient and regioselective biocatalytic synthesis of aromatic N-oxides by using a soluble di-iron monooxygenase PmlABCDEF produced in the Pseudomonas species. | Petkevicius V, Vaitekunas J, Gasparaviciute R, Tauraite D, Meskys R. | Microb Biotechnol | 10.1111/1751-7915.13849 | 2021 | | |
| Genetics | Dataset on phenotypic characterization, on protein and genome analysis of three fluorescent Pseudomonas strains from mid-mountain water. | Dussert E, Tourret M, Deracinois B, Duban M, Leclere V, Cudennec B, Ravallec R, Behra-Miellet J. | Data Brief | 10.1016/j.dib.2020.105466 | 2020 | |
| Metabolism | Morphological and Metabolite Responses of Potatoes under Various Phosphorus Levels and Their Amelioration by Plant Growth-Promoting Rhizobacteria. | Chea L, Pfeiffer B, Schneider D, Daniel R, Pawelzik E, Naumann M. | Int J Mol Sci | 10.3390/ijms22105162 | 2021 | |
| Engineering Pseudomonas putida KT2440 for the production of isobutanol. | Nitschel R, Ankenbauer A, Welsch I, Wirth NT, Massner C, Ahmad N, McColm S, Borges F, Fotheringham I, Takors R, Blombach B. | Eng Life Sci | 10.1002/elsc.201900151 | 2020 | | |
| Metabolism | Vanillin Production in Pseudomonas: Whole-Genome Sequencing of Pseudomonas sp. Strain 9.1 and Reannotation of Pseudomonas putida CalA as a Vanillin Reductase. | Garcia-Hidalgo J, Brink DP, Ravi K, Paul CJ, Liden G, Gorwa-Grauslund MF. | Appl Environ Microbiol | 10.1128/aem.02442-19 | 2020 | |
| Metabolic engineering of Pseudomonas putida for production of vanillylamine from lignin-derived substrates. | Manfrao-Netto JHC, Lund F, Muratovska N, Larsson EM, Parachin NS, Carlquist M. | Microb Biotechnol | 10.1111/1751-7915.13764 | 2021 | | |
| Biotechnology | Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production. | Askitosari TD, Berger C, Tiso T, Harnisch F, Blank LM, Rosenbaum MA. | Microorganisms | 10.3390/microorganisms8121959 | 2020 | |
| Changes in growth, lanthanide binding, and gene expression in Pseudomonas alloputida KT2440 in response to light and heavy lanthanides. | Gorniak L, Bucka SL, Nasr B, Cao J, Hellmann S, Schafer T, Westermann M, Bechwar J, Wegner C-E. | mSphere | 10.1128/msphere.00685-24 | 2024 | | |
| Pseudomonas putida KT2440 is naturally endowed to withstand industrial-scale stress conditions. | Ankenbauer A, Schafer RA, Viegas SC, Pobre V, Voss B, Arraiano CM, Takors R. | Microb Biotechnol | 10.1111/1751-7915.13571 | 2020 | | |
| Osmotic stress in colony and planktonic cells of Pseudomonas putida mt-2 revealed significant differences in adaptive response mechanisms. | Hachicho N, Birnbaum A, Heipieper HJ. | AMB Express | 10.1186/s13568-017-0371-8 | 2017 | | |
| Development and Characterization of Bioactive Polypropylene Films for Food Packaging Applications. | Antosik AK, Kowalska U, Stobinska M, Dzieciol P, Pieczykolan M, Kozlowska K, Bartkowiak A. | Polymers (Basel) | 10.3390/polym13203478 | 2021 | | |
| Quantitation and Comparison of Phenotypic Heterogeneity Among Single Cells of Monoclonal Microbial Populations. | Calabrese F, Voloshynovska I, Musat F, Thullner M, Schlomann M, Richnow HH, Lambrecht J, Muller S, Wick LY, Musat N, Stryhanyuk H. | Front Microbiol | 10.3389/fmicb.2019.02814 | 2019 | | |
| Boosting Heterologous Phenazine Production in Pseudomonas putida KT2440 Through the Exploration of the Natural Sequence Space. | Askitosari TD, Boto ST, Blank LM, Rosenbaum MA. | Front Microbiol | 10.3389/fmicb.2019.01990 | 2019 | | |
| Metabolic Engineering of Pseudomonas putida KT2440 for the Production of para-Hydroxy Benzoic Acid. | Yu S, Plan MR, Winter G, Kromer JO. | Front Bioeng Biotechnol | 10.3389/fbioe.2016.00090 | 2016 | | |
| Metabolism | Characterization of highly active 2-keto-3-deoxy-L-arabinonate and 2-keto-3-deoxy-D-xylonate dehydratases in terms of the biotransformation of hemicellulose sugars to chemicals. | Sutiono S, Siebers B, Sieber V. | Appl Microbiol Biotechnol | 10.1007/s00253-020-10742-5 | 2020 | |
| Metabolism | Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost. | Ravi K, Garcia-Hidalgo J, Gorwa-Grauslund MF, Liden G. | Appl Microbiol Biotechnol | 10.1007/s00253-017-8211-y | 2017 | |
| Metabolism | A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production. | Borrero-de Acuna JM, Hidalgo-Dumont C, Pacheco N, Cabrera A, Poblete-Castro I. | Sci Rep | 10.1038/s41598-017-04741-2 | 2017 | |
| Genome-wide identification of tolerance mechanisms toward p-coumaric acid in Pseudomonas putida. | Calero P, Jensen SI, Bojanovic K, Lennen RM, Koza A, Nielsen AT. | Biotechnol Bioeng | 10.1002/bit.26495 | 2018 | | |
| Metabolism | Nitrogen regulation of the xyl genes of Pseudomonas putida mt-2 propagates into a significant effect of nitrate on m-xylene mineralization in soil. | Svenningsen NB, Nicolaisen MH, Hansen HC, de Lorenzo V, Nybroe O. | Microb Biotechnol | 10.1111/1751-7915.12404 | 2016 | |
| Genetics | Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids. | Volke DC, Wirth NT, Nikel PI. | Bio Protoc | 10.21769/bioprotoc.3917 | 2021 | |
| Enhancing curcumin's solubility and antibiofilm activity via silica surface modification. | Barros CHN, Devlin H, Hiebner DW, Vitale S, Quinn L, Casey E. | Nanoscale Adv | 10.1039/d0na00041h | 2020 | | |
| Metabolism | Comparison of mcl-Poly(3-hydroxyalkanoates) synthesis by different Pseudomonas putida strains from crude glycerol: citrate accumulates at high titer under PHA-producing conditions. | Poblete-Castro I, Binger D, Oehlert R, Rohde M. | BMC Biotechnol | 10.1186/s12896-014-0110-z | 2014 | |
| Genetics | Genomotyping of Pseudomonas putida strains using P. putida KT2440-based high-density DNA microarrays: implications for transcriptomics studies. | Ballerstedt H, Volkers RJ, Mars AE, Hallsworth JE, dos Santos VA, Puchalka J, van Duuren J, Eggink G, Timmis KN, de Bont JA, Wery J. | Appl Microbiol Biotechnol | 10.1007/s00253-007-0914-z | 2007 | |
| Metabolism | Pseudomonas putida mt-2 tolerates reactive oxygen species generated during matric stress by inducing a major oxidative defense response. | Svenningsen NB, Perez-Pantoja D, Nikel PI, Nicolaisen MH, de Lorenzo V, Nybroe O. | BMC Microbiol | 10.1186/s12866-015-0542-1 | 2015 | |
| Knockout of extracytoplasmic function sigma factor ECF-10 affects stress resistance and biofilm formation in Pseudomonas putida KT2440. | Tettmann B, Dotsch A, Armant O, Fjell CD, Overhage J. | Appl Environ Microbiol | 10.1128/aem.01291-14 | 2014 | | |
| Bacterial conversion of depolymerized Kraft lignin. | Ravi K, Abdelaziz OY, Nobel M, Garcia-Hidalgo J, Gorwa-Grauslund MF, Hulteberg CP, Liden G. | Biotechnol Biofuels | 10.1186/s13068-019-1397-8 | 2019 | | |
| Metabolism | Novel insights into biosynthesis and uptake of rhamnolipids and their precursors. | Wittgens A, Kovacic F, Muller MM, Gerlitzki M, Santiago-Schubel B, Hofmann D, Tiso T, Blank LM, Henkel M, Hausmann R, Syldatk C, Wilhelm S, Rosenau F. | Appl Microbiol Biotechnol | 10.1007/s00253-016-8041-3 | 2017 | |
| Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. | Dammeyer T, Steinwand M, Kruger SC, Dubel S, Hust M, Timmis KN. | Microb Cell Fact | 10.1186/1475-2859-10-11 | 2011 | | |
| Metabolism | Characterizing a thermostable Cas9 for bacterial genome editing and silencing. | Mougiakos I, Mohanraju P, Bosma EF, Vrouwe V, Finger Bou M, Naduthodi MIS, Gussak A, Brinkman RBL, van Kranenburg R, van der Oost J. | Nat Commun | 10.1038/s41467-017-01591-4 | 2017 | |
| Engineering mediator-based electroactivity in the obligate aerobic bacterium Pseudomonas putida KT2440. | Schmitz S, Nies S, Wierckx N, Blank LM, Rosenbaum MA. | Front Microbiol | 10.3389/fmicb.2015.00284 | 2015 | | |
| Genetics | In-Depth Genomic and Phenotypic Characterization of the Antarctic Psychrotolerant Strain Pseudomonas sp. MPC6 Reveals Unique Metabolic Features, Plasticity, and Biotechnological Potential. | Orellana-Saez M, Pacheco N, Costa JI, Mendez KN, Miossec MJ, Meneses C, Castro-Nallar E, Marcoleta AE, Poblete-Castro I. | Front Microbiol | 10.3389/fmicb.2019.01154 | 2019 | |
| Metabolism | Broad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein. | Dammeyer T, Timmis KN, Tinnefeld P. | Microb Cell Fact | 10.1186/1475-2859-12-49 | 2013 | |
| Metabolism | Growth of Pseudomonas chloritidismutans AW-1(T) on n-alkanes with chlorate as electron acceptor. | Mehboob F, Junca H, Schraa G, Stams AJ. | Appl Microbiol Biotechnol | 10.1007/s00253-009-1985-9 | 2009 | |
| Genetics | Functional genomics of stress response in Pseudomonas putida KT2440. | Reva ON, Weinel C, Weinel M, Bohm K, Stjepandic D, Hoheisel JD, Tummler B. | J Bacteriol | 10.1128/jb.00101-06 | 2006 | |
| Metabolism | Large-Scale 13C flux profiling reveals conservation of the Entner-Doudoroff pathway as a glycolytic strategy among marine bacteria that use glucose. | Klingner A, Bartsch A, Dogs M, Wagner-Dobler I, Jahn D, Simon M, Brinkhoff T, Becker J, Wittmann C. | Appl Environ Microbiol | 10.1128/aem.03157-14 | 2015 | |
| Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery. | Ruhl IA, Woodworth SP, Haugen SJ, Alt HM, Beckham GT, Johnson CW. | Microb Biotechnol | 10.1111/1751-7915.70152 | 2025 | | |
| A distinct subpopulation of membrane vesicles in Pseudomonas putida is enriched in enzymes for lignin catabolism. | Werner AZ, Giannone RJ, Keller MJ, Plavchak C, Carper DL, Abraham PE, Wilkes RA, Aristilde L, Salvachua D, Ratanathanawongs Williams SK, Hettich RL, Beckham GT. | Appl Environ Microbiol | 10.1128/aem.01617-25 | 2025 | | |
| Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida. | Wilkes RA, Miller TE, Waldbauer J, Zhou N, Zhang L, DiBiase BN, Kamat NP, Aristilde L, Beckham GT, Werner AZ. | ACS Synth Biol | 10.1021/acssynbio.5c00171 | 2025 | | |
| A metabolic engineering strategy for producing poly-(3-hydroxyoctanoic acid) in Escherichia coli from glycerol. | Mishra S, Xu K, Kuckuk MK, Cordell WT, Hernandez-Lozada NJ, Pfleger BF. | Metab Eng | 10.1016/j.ymben.2025.08.009 | 2025 | | |
| Isolation, characterization, and application of bacteriophage cocktails for the biocontrol of Pseudomonas fluorescens group strains in whole and skimmed milk. | Tayyarcan EK, Boyaci IH. | Braz J Microbiol | 10.1007/s42770-023-01164-2 | 2023 | | |
| Five tree species contained antibiotic-producing bacteria within their bark. | Hoffman C, Blanke K. | MicroPubl Biol | 10.17912/micropub.biology.001227 | 2024 | | |
| Alternate routes to acetate tolerance lead to varied isoprenol production from mixed carbon sources in Pseudomonas putida. | de Siqueira GMV, Srinivasan A, Chen Y, Gin JW, Petzold CJ, Lee TS, Guazzaroni M-E, Eng T, Mukhopadhyay A. | Appl Environ Microbiol | 10.1128/aem.02123-24 | 2025 | | |
| Conjugative type IV secretion systems enable bacterial antagonism that operates independently of plasmid transfer. | Gordils-Valentin L, Ouyang H, Qian L, Hong J, Zhu X. | Commun Biol | 10.1038/s42003-024-06192-8 | 2024 | | |
| Cultivation | Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida. | Zournas A, Incha MR, Radivojevic T, Blay V, Marti JM, Costello Z, Schmidt M, Chung T, Thompson MG, Pearson A, Kinnunen PC, Eng T, Lawson CE, Tan S, Ogorzalek T, Kaplan N, Forrer M, Backman T, Mukhopadhyay A, Hillson NJ, Keasling JD, Garcia Martin H. | Commun Biol | 10.1038/s42003-025-08039-2 | 2025 | |
| Upcycling waste polystyrene to adipic acid through a hybrid chemical and biological process. | Moon H, DesVeaux JS, Van Roijen EC, Singer CA, Alt HM, Konev MO, Lincoln C, Avina YC, Meyer NR, Haugen SJ, Lee H, An K, Miscall J, Ramirez KJ, Johnson CW, Salvachua D, Klein BC, Uekert T, Werner AZ, Stahl SS, Beckham GT. | Nat Commun | 10.1038/s41467-025-64561-1 | 2025 | | |
| Biomimetic layered, ecological, advanced, multi-functional film for sustainable packaging. | Dhatt PS, Hu A, Hu C, Huynh V, Dai SY, Yuan JS. | Nat Commun | 10.1038/s41467-025-61693-2 | 2025 | | |
| A Genetically Encoded Biosensor for the Detection of Levulinic Acid. | Kim TH, Woo SG, Kim SK, Yoo BH, Shin J, Rha E, Kim SJ, Kwon KK, Lee H, Kim H, Kim HT, Sung BH, Lee SG, Lee DH. | J Microbiol Biotechnol | 10.4014/jmb.2301.01021 | 2023 | | |
| Lignin conversion to beta-ketoadipic acid by Pseudomonas putida via metabolic engineering and bioprocess development. | Werner AZ, Cordell WT, Lahive CW, Klein BC, Singer CA, Tan ECD, Ingraham MA, Ramirez KJ, Kim DH, Pedersen JN, Johnson CW, Pfleger BF, Beckham GT, Salvachua D. | Sci Adv | 10.1126/sciadv.adj0053 | 2023 | | |
| Genetic Code Expansion in Pseudomonas putida KT2440. | He X, Gao T, Chen Y, Liu K, Guo J, Niu W. | ACS Synth Biol | 10.1021/acssynbio.2c00325 | 2022 | | |
| Engineering Pseudomonas putida for improved utilization of syringyl aromatics. | Mueller J, Willett H, Feist AM, Niu W. | Biotechnol Bioeng | 10.1002/bit.28131 | 2022 | | |
| The pGinger Family of Expression Plasmids. | Pearson AN, Thompson MG, Kirkpatrick LD, Ho C, Vuu KM, Waldburger LM, Keasling JD, Shih PM. | Microbiol Spectr | 10.1128/spectrum.00373-23 | 2023 | | |
| Biotechnology | Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria. | Keating KW, Young EM. | ACS Synth Biol | 10.1021/acssynbio.3c00104 | 2023 | |
| Evolution of the Tn4371 ICE family: traR-mediated coordination of cargo gene upregulation and horizontal transfer. | Matsumoto S, Kishida K, Nonoyama S, Sakai K, Tsuda M, Nagata Y, Ohtsubo Y. | Microbiol Spectr | 10.1128/spectrum.00607-24 | 2024 | | |
| Understanding of bacterial lignin extracellular degradation mechanisms by Pseudomonas putida KT2440 via secretomic analysis. | Xu Z, Peng B, Kitata RB, Nicora CD, Weitz KK, Pu Y, Shi T, Cort JR, Ragauskas AJ, Yang B. | Biotechnol Biofuels Bioprod | 10.1186/s13068-022-02214-x | 2022 | | |
| Environmental DNA transformation resulted in an active phage in Escherichia coli. | Karaynir A, Bozdogan B, Salih Dogan H. | PLoS One | 10.1371/journal.pone.0292933 | 2023 | | |
| A new understanding of Acanthamoeba castellanii: dispelling the role of bacterial pore-forming toxins in cyst formation and amoebicidal actions. | Yabrag A, Ullah N, Baryalai P, Ahmad I, Zlatkov N, Toh E, Lindback T, Uhlin BE, Wai SN, Nadeem A. | Cell Death Discov | 10.1038/s41420-025-02345-8 | 2025 | | |
| Synthetic microbe-to-plant communication channels. | Boo A, Toth T, Yu Q, Pfotenhauer A, Fields BD, Lenaghan SC, Stewart CN, Voigt CA. | Nat Commun | 10.1038/s41467-024-45897-6 | 2024 | | |
| Enzymology | Blocking bacterial appendage attachment to wastewater treatment membranes using anti-adhesins. | Zamora R, McEvoy J, Colbert C, Chacana Olivares J, Kaewlom P, Khan E. | Chemosphere | 10.1016/j.chemosphere.2023.138246 | 2023 | |
| Precise Genomic Riboregulator Control of Metabolic Flux in Microbial Systems. | Pandey N, Davison SA, Krishnamurthy M, Trettel DS, Lo CC, Starkenburg S, Wozniak KL, Kern TL, Reardon SD, Unkefer CJ, Hennelly SP, Dale T. | ACS Synth Biol | 10.1021/acssynbio.1c00638 | 2022 | | |
| Metabolism | Creatine utilization as a sole nitrogen source in Pseudomonas putida KT2440 is transcriptionally regulated by CahR. | Hinkel LA, Willsey GG, Lenahan SM, Eckstrom K, Schutz KC, Wargo MJ. | Microbiology (Reading) | 10.1099/mic.0.001145 | 2022 | |
| Metabolism | Lactic acid containing polymers produced in engineered Sinorhizobium meliloti and Pseudomonas putida. | Tran TT, Charles TC. | PLoS One | 10.1371/journal.pone.0218302 | 2020 | |
| Reconstruction and optimization of a Pseudomonas putida-Escherichia coli microbial consortium for mcl-PHA production from lignocellulosic biomass. | Qin R, Zhu Y, Ai M, Jia X. | Front Bioeng Biotechnol | 10.3389/fbioe.2022.1023325 | 2022 | | |
| Enzymology | The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440. | Wehrmann M, Berthelot C, Billard P, Klebensberger J. | mSphere | 10.1128/msphere.00376-18 | 2018 | |
| Pathogenicity | Genomic Background Governs Opposing Responses to Nalidixic Acid upon Megaplasmid Acquisition in Pseudomonas. | Baltrus DA, Smith C, Derrick M, Leligdon C, Rosenthal Z, Mollico M, Moore A, Clark M. | mSphere | 10.1128/msphere.00008-21 | 2021 | |
| Pathogenicity | Effect of rhamnolipid on the physicochemical properties and interaction of bacteria and fungi. | Hamzah N, Kasmuri N, Tao W, Singhal N, Padhye L, Swift S. | Braz J Microbiol | 10.1007/s42770-020-00295-0 | 2020 | |
| Metabolism | Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440. | Salvachua D, Werner AZ, Pardo I, Michalska M, Black BA, Donohoe BS, Haugen SJ, Katahira R, Notonier S, Ramirez KJ, Amore A, Purvine SO, Zink EM, Abraham PE, Giannone RJ, Poudel S, Laible PD, Hettich RL, Beckham GT. | Proc Natl Acad Sci U S A | 10.1073/pnas.1921073117 | 2020 | |
| Leveraging host metabolism for bisdemethoxycurcumin production in Pseudomonas putida. | Incha MR, Thompson MG, Blake-Hedges JM, Liu Y, Pearson AN, Schmidt M, Gin JW, Petzold CJ, Deutschbauer AM, Keasling JD. | Metab Eng Commun | 10.1016/j.mec.2019.e00119 | 2020 | | |
| Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440. | Xu Z, Pan C, Li X, Hao N, Zhang T, Gaffrey MJ, Pu Y, Cort JR, Ragauskas AJ, Qian WJ, Yang B. | Biotechnol Biofuels | 10.1186/s13068-020-01861-2 | 2021 | | |
| Metabolism | Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing. | Schmidt M, Pearson AN, Incha MR, Thompson MG, Baidoo EEK, Kakumanu R, Mukhopadhyay A, Shih PM, Deutschbauer AM, Blank LM, Keasling JD. | Appl Environ Microbiol | 10.1128/aem.02430-21 | 2022 | |
| Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer. | Thompson MG, Valencia LE, Blake-Hedges JM, Cruz-Morales P, Velasquez AE, Pearson AN, Sermeno LN, Sharpless WA, Benites VT, Chen Y, Baidoo EEK, Petzold CJ, Deutschbauer AM, Keasling JD. | Metab Eng Commun | 10.1016/j.mec.2019.e00098 | 2019 | | |
| Microbial and Enzymatic Degradation of Synthetic Plastics. | Mohanan N, Montazer Z, Sharma PK, Levin DB. | Front Microbiol | 10.3389/fmicb.2020.580709 | 2020 | | |
| Optimization of a Two-Species Microbial Consortium for Improved Mcl-PHA Production From Glucose-Xylose Mixtures. | Zhu Y, Ai M, Jia X. | Front Bioeng Biotechnol | 10.3389/fbioe.2021.794331 | 2021 | | |
| A protocatechuate biosensor for Pseudomonas putida KT2440 via promoter and protein evolution. | Jha RK, Bingen JM, Johnson CW, Kern TL, Khanna P, Trettel DS, Strauss CEM, Beckham GT, Dale T. | Metab Eng Commun | 10.1016/j.meteno.2018.03.001 | 2018 | | |
| Metabolism | Hierarchical routing in carbon metabolism favors iron-scavenging strategy in iron-deficient soil Pseudomonas species. | Mendonca CM, Yoshitake S, Wei H, Werner A, Sasnow SS, Thannhauser TW, Aristilde L. | Proc Natl Acad Sci U S A | 10.1073/pnas.2016380117 | 2020 | |
| Metabolism | Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos. | Gong T, Liu R, Che Y, Xu X, Zhao F, Yu H, Song C, Liu Y, Yang C. | Microb Biotechnol | 10.1111/1751-7915.12381 | 2016 | |
| Metabolism | Fatty Acid and Alcohol Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing. | Thompson MG, Incha MR, Pearson AN, Schmidt M, Sharpless WA, Eiben CB, Cruz-Morales P, Blake-Hedges JM, Liu Y, Adams CA, Haushalter RW, Krishna RN, Lichtner P, Blank LM, Mukhopadhyay A, Deutschbauer AM, Shih PM, Keasling JD. | Appl Environ Microbiol | 10.1128/aem.01665-20 | 2020 | |
| Determining the Different Mechanisms Used by Pseudomonas Species to Cope With Minimal Inhibitory Concentrations of Zinc via Comparative Transcriptomic Analyses. | Lei L, Chen J, Liao W, Liu P. | Front Microbiol | 10.3389/fmicb.2020.573857 | 2020 | | |
| The Bistable Behaviour of Pseudomonas putida KT2440 during PHA Depolymerization under Carbon Limitation. | Karmann S, Panke S, Zinn M. | Bioengineering (Basel) | 10.3390/bioengineering4020058 | 2017 | | |
| Reproducible growth of Brachypodium in EcoFAB 2.0 reveals that nitrogen form and starvation modulate root exudation. | Novak V, Andeer PF, Bowen BP, Ding Y, Zhalnina K, Hofmockel KS, Tomaka C, Harwood TV, van Winden MCM, Golini AN, Kosina SM, Northen TR. | Sci Adv | 10.1126/sciadv.adg7888 | 2024 | | |
| Metabolism | Development of a CRISPR/Cas9n-based tool for metabolic engineering of Pseudomonas putida for ferulic acid-to-polyhydroxyalkanoate bioconversion. | Zhou Y, Lin L, Wang H, Zhang Z, Zhou J, Jiao N. | Commun Biol | 10.1038/s42003-020-0824-5 | 2020 | |
| Chronic gamma radiation resistance in fungi correlates with resistance to chromium and elevated temperatures, but not with resistance to acute irradiation. | Shuryak I, Tkavc R, Matrosova VY, Volpe RP, Grichenko O, Klimenkova P, Conze IH, Balygina IA, Gaidamakova EK, Daly MJ. | Sci Rep | 10.1038/s41598-019-47007-9 | 2019 | | |
| Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440. | Guarnieri MT, Ann Franden M, Johnson CW, Beckham GT. | Metab Eng Commun | 10.1016/j.meteno.2017.02.001 | 2017 | | |
| Metabolism | Combinatorial metabolic engineering of Pseudomonas putida KT2440 for efficient mineralization of 1,2,3-trichloropropane. | Gong T, Xu X, Che Y, Liu R, Gao W, Zhao F, Yu H, Liang J, Xu P, Song C, Yang C. | Sci Rep | 10.1038/s41598-017-07435-x | 2017 | |
| Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs. | Cho JS, Yang D, Prabowo CPS, Ghiffary MR, Han T, Choi KR, Moon CW, Zhou H, Ryu JY, Kim HU, Lee SY. | Nat Commun | 10.1038/s41467-023-38119-y | 2023 | | |
| Bacterial survival in microscopic surface wetness. | Grinberg M, Orevi T, Steinberg S, Kashtan N. | Elife | 10.7554/elife.48508 | 2019 | | |
| Biological Control of Verticillium Wilt on Olive Trees by the Salt-Tolerant Strain Bacillus velezensis XT1. | Castro D, Torres M, Sampedro I, Martinez-Checa F, Torres B, Bejar V. | Microorganisms | 10.3390/microorganisms8071080 | 2020 | | |
| Metabolism | Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in Pseudomonas putida Lysine Metabolism. | Thompson MG, Blake-Hedges JM, Cruz-Morales P, Barajas JF, Curran SC, Eiben CB, Harris NC, Benites VT, Gin JW, Sharpless WA, Twigg FF, Skyrud W, Krishna RN, Pereira JH, Baidoo EEK, Petzold CJ, Adams PD, Arkin AP, Deutschbauer AM, Keasling JD. | mBio | 10.1128/mbio.02577-18 | 2019 | |
| Metabolism | Identification and Characterization of a Novel pic Gene Cluster Responsible for Picolinic Acid Degradation in Alcaligenes faecalis JQ135. | Qiu J, Zhao L, Xu S, Chen Q, Chen L, Liu B, Hong Q, Lu Z, He J. | J Bacteriol | 10.1128/jb.00077-19 | 2019 | |
| Accelerating pathway evolution by increasing the gene dosage of chromosomal segments. | Tumen-Velasquez M, Johnson CW, Ahmed A, Dominick G, Fulk EM, Khanna P, Lee SA, Schmidt AL, Linger JG, Eiteman MA, Beckham GT, Neidle EL. | Proc Natl Acad Sci U S A | 10.1073/pnas.1803745115 | 2018 | | |
| Metabolism | Anaerobic production of medium-chain fatty alcohols via a beta-reduction pathway. | Mehrer CR, Incha MR, Politz MC, Pfleger BF. | Metab Eng | 10.1016/j.ymben.2018.05.011 | 2018 | |
| Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production. | Bedade DK, Edson CB, Gross RA. | Molecules | 10.3390/molecules26113463 | 2021 | | |
| Metabolism | Measurement of soil bacterial colony temperatures and isolation of a high heat-producing bacterium. | Tabata K, Hida F, Kiriyama T, Ishizaki N, Kamachi T, Okura I. | BMC Microbiol | 10.1186/1471-2180-13-56 | 2013 | |
| Metabolism | Characterization of l-Carnitine Metabolism in Sinorhizobium meliloti. | Bazire P, Perchat N, Darii E, Lechaplais C, Salanoubat M, Perret A. | J Bacteriol | 10.1128/jb.00772-18 | 2019 | |
| Genetics | Subpopulation-proteomics reveal growth rate, but not cell cycling, as a major impact on protein composition in Pseudomonas putida KT2440. | Lieder S, Jahn M, Seifert J, von Bergen M, Muller S, Takors R. | AMB Express | 10.1186/s13568-014-0071-6 | 2014 | |
| Microbial Ecotoxicology of Marine Plastic Debris: A Review on Colonization and Biodegradation by the "Plastisphere". | Jacquin J, Cheng J, Odobel C, Pandin C, Conan P, Pujo-Pay M, Barbe V, Meistertzheim AL, Ghiglione JF. | Front Microbiol | 10.3389/fmicb.2019.00865 | 2019 | | |
| Development of a method for markerless gene deletion in Pseudomonas putida. | Graf N, Altenbuchner J. | Appl Environ Microbiol | 10.1128/aem.05055-11 | 2011 | | |
| Atomic force microscopy measurements of bacterial adhesion and biofilm formation onto clay-sized particles. | Huang Q, Wu H, Cai P, Fein JB, Chen W. | Sci Rep | 10.1038/srep16857 | 2015 | | |
| 2D motility tracking of Pseudomonas putida KT2440 in growth phases using video microscopy. | Davis ML, Mounteer LC, Stevens LK, Miller CD, Zhou A. | J Biosci Bioeng | 10.1016/j.jbiosc.2011.01.007 | 2011 | | |
| Metabolism | Ethylene glycol metabolism by Pseudomonas putida. | Muckschel B, Simon O, Klebensberger J, Graf N, Rosche B, Altenbuchner J, Pfannstiel J, Huber A, Hauer B. | Appl Environ Microbiol | 10.1128/aem.02062-12 | 2012 | |
| Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity. | Johnson CW, Salvachua D, Khanna P, Smith H, Peterson DJ, Beckham GT. | Metab Eng Commun | 10.1016/j.meteno.2016.04.002 | 2016 | | |
| Genetically Encoded Photosensitizers as Light-Triggered Antimicrobial Agents. | Hilgers F, Bitzenhofer NL, Ackermann Y, Burmeister A, Grunberger A, Jaeger KE, Drepper T. | Int J Mol Sci | 10.3390/ijms20184608 | 2019 | | |
| Fed-batch production of MCL-PHA with elevated 3-hydroxynonanoate content. | Jiang XJ, Sun Z, Ramsay JA, Ramsay BA. | AMB Express | 10.1186/2191-0855-3-50 | 2013 | | |
| Metabolism | Across the tree of life, radiation resistance is governed by antioxidant Mn2+, gauged by paramagnetic resonance. | Sharma A, Gaidamakova EK, Grichenko O, Matrosova VY, Hoeke V, Klimenkova P, Conze IH, Volpe RP, Tkavc R, Gostincar C, Gunde-Cimerman N, DiRuggiero J, Shuryak I, Ozarowski A, Hoffman BM, Daly MJ. | Proc Natl Acad Sci U S A | 10.1073/pnas.1713608114 | 2017 | |
| Genetics | Recent advances of Cas12a applications in bacteria. | Meliawati M, Schilling C, Schmid J. | Appl Microbiol Biotechnol | 10.1007/s00253-021-11243-9 | 2021 | |
| Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol. | Gascoyne JL, Bommareddy RR, Heeb S, Malys N. | Metab Eng | 10.1016/j.ymben.2021.06.010 | 2021 | | |
| Metabolism | The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis. | Lenz P, Hilgers F, Burmeister A, Zimmermann L, Volkenborn K, Grunberger A, Kohlheyer D, Drepper T, Jaeger KE, Knapp A. | Microb Cell Fact | 10.1186/s12934-021-01663-7 | 2021 | |
| Challenges with Verifying Microbial Degradation of Polyethylene. | Montazer Z, Habibi Najafi MB, Levin DB. | Polymers (Basel) | 10.3390/polym12010123 | 2020 | | |
| Metabolism | Further characterization of o-nitrobenzaldehyde degrading bacterium Pseudomonas sp. ONBA-17 and deduction on its metabolic pathway. | Yu FB, Li XD, Ali SW, Shan SD, Luo LP, Guan LB. | Braz J Microbiol | 10.1590/s1517-83822014000400021 | 2014 | |
| Enzymology | Improved Expression and Optimization of Trehalose Synthase by Regulation of Pglv in Bacillus subtilis. | Liu H, Liu H, Yang S, Wang R, Wang T. | Sci Rep | 10.1038/s41598-019-43172-z | 2019 | |
| A portable expression resource for engineering cross-species genetic circuits and pathways. | Kushwaha M, Salis HM. | Nat Commun | 10.1038/ncomms8832 | 2015 | | |
| Metabolism | Integrated bioprocessing for the pH-dependent production of 4-valerolactone from levulinate in Pseudomonas putida KT2440. | Martin CH, Wu D, Prather KL. | Appl Environ Microbiol | 10.1128/aem.01769-09 | 2010 | |
| Enzymology | Site-directed mutagenesis identifies a molecular switch involved in copper sensing by the histidine kinase CinS in Pseudomonas putida KT2440. | Quaranta D, McEvoy MM, Rensing C. | J Bacteriol | 10.1128/jb.00551-09 | 2009 | |
| Metabolism | Production of medium chain length fatty alcohols from glucose in Escherichia coli. | Youngquist JT, Schumacher MH, Rose JP, Raines TC, Politz MC, Copeland MF, Pfleger BF. | Metab Eng | 10.1016/j.ymben.2013.10.006 | 2013 | |
| Enzymology | Cloning and characterization of uronate dehydrogenases from two pseudomonads and Agrobacterium tumefaciens strain C58. | Yoon SH, Moon TS, Iranpour P, Lanza AM, Prather KJ. | J Bacteriol | 10.1128/jb.00586-08 | 2009 | |
| Enzymology | Bacterial ammeline metabolism via guanine deaminase. | Seffernick JL, Dodge AG, Sadowsky MJ, Bumpus JA, Wackett LP. | J Bacteriol | 10.1128/jb.01243-09 | 2010 | |
| Metabolism | Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids. | Yano H, Miyakoshi M, Ohshima K, Tabata M, Nagata Y, Hattori M, Tsuda M. | J Bacteriol | 10.1128/jb.00359-10 | 2010 | |
| Metabolism | The copper-inducible cin operon encodes an unusual methionine-rich azurin-like protein and a pre-Q0 reductase in Pseudomonas putida KT2440. | Quaranta D, McCarty R, Bandarian V, Rensing C. | J Bacteriol | 10.1128/jb.00377-07 | 2007 | |
| Metabolism | Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa. | Forster-Fromme K, Hoschle B, Mack C, Bott M, Armbruster W, Jendrossek D. | Appl Environ Microbiol | 10.1128/aem.00853-06 | 2006 | |
| Metabolism | Conjugal transfer of polychlorinated biphenyl/biphenyl degradation genes in Acidovorax sp. strain KKS102, which are located on an integrative and conjugative element. | Ohtsubo Y, Ishibashi Y, Naganawa H, Hirokawa S, Atobe S, Nagata Y, Tsuda M. | J Bacteriol | 10.1128/jb.00352-12 | 2012 | |
| Phylogeny | Direct detection of nasal Staphylococcus aureus carriage via helicase-dependent isothermal amplification and chip hybridization. | Frech GC, Munns D, Jenison RD, Hicke BJ. | BMC Res Notes | 10.1186/1756-0500-5-430 | 2012 | |
| Evaluation of shotgun metagenomics sequence classification methods using in silico and in vitro simulated communities. | Peabody MA, Van Rossum T, Lo R, Brinkman FS. | BMC Bioinformatics | 10.1186/s12859-015-0788-5 | 2015 | | |
| Pathogenicity | Host range diversification within the IncP-1 plasmid group. | Yano H, Rogers LM, Knox MG, Heuer H, Smalla K, Brown CJ, Top EM. | Microbiology (Reading) | 10.1099/mic.0.068387-0 | 2013 | |
| Metabolism | Roles of long and short replication initiation proteins in the fate of IncP-1 plasmids. | Yano H, Deckert GE, Rogers LM, Top EM. | J Bacteriol | 10.1128/jb.06395-11 | 2012 | |
| Metabolism | Small-molecule antioxidant proteome-shields in Deinococcus radiodurans. | Daly MJ, Gaidamakova EK, Matrosova VY, Kiang JG, Fukumoto R, Lee DY, Wehr NB, Viteri GA, Berlett BS, Levine RL. | PLoS One | 10.1371/journal.pone.0012570 | 2010 | |
| Metabolism | Protein oxidation implicated as the primary determinant of bacterial radioresistance. | Daly MJ, Gaidamakova EK, Matrosova VY, Vasilenko A, Zhai M, Leapman RD, Lai B, Ravel B, Li SM, Kemner KM, Fredrickson JK. | PLoS Biol | 10.1371/journal.pbio.0050092 | 2007 | |
| Conjugative plasmids isolated from bacteria in marine environments show various degrees of homology to each other and are not closely related to well-characterized plasmids. | Dahlberg C, Linberg C, Torsvik VL, Hermansson M. | Appl Environ Microbiol | 10.1128/aem.63.12.4692-4697.1997 | 1997 | | |
| Evaluation of 3D-Printing Scaffold Fabrication on Biosynthetic Medium-Chain-Length Polyhydroxyalkanoate Terpolyester as Biomaterial-Ink. | Panaksri A, Tanadchangsaeng N | Polymers (Basel) | 10.3390/polym13142222 | 2021 | | |
| Metabolism | Valorization of Gelidium amansii for dual production of D-galactonic acid and 5-hydroxymethyl-2-furancarboxylic acid by chemo-biological approach. | Liu P, Xie J, Tan H, Zhou F, Zou L, Ouyang J | Microb Cell Fact | 10.1186/s12934-020-01357-6 | 2020 | |
| Enzymology | A process for production of trehalose by recombinant trehalose synthase and its purification. | Liu H, Yang S, Liu Q, Wang R, Wang T | Enzyme Microb Technol | 10.1016/j.enzmictec.2017.11.008 | 2017 | |
| Cell-Surface Displayed Expression of Trehalose Synthase from Pseudomonas putida ATCC 47054 in Pichia Pastoris Using Pir1p as an Anchor Protein. | Yang S, Lv X, Wang X, Wang J, Wang R, Wang T | Front Microbiol | 10.3389/fmicb.2017.02583 | 2017 | | |
| Metabolism | Biodegradation of Low Density Polythene (LDPE) by Pseudomonas Species. | Kyaw BM, Champakalakshmi R, Sakharkar MK, Lim CS, Sakharkar KR | Indian J Microbiol | 10.1007/s12088-012-0250-6 | 2012 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive12896.20251217.10
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BacDive in 2025: the core database for prokaryotic strain data
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