Strain identifier

BacDive ID: 12896

Type strain: No

Species: Pseudomonas putida

Strain Designation: mt-2 KT2440

Strain history: <- CSIC <- K.N. Timmis

NCBI tax ID(s): 303 (species)

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General

@ref: 2385

BacDive-ID: 12896

DSM-Number: 6125

keywords: Bacteria, mesophilic

description: Pseudomonas putida mt-2 KT2440 is a mesophilic bacterium that was isolated from Safety host for recombinant DNA experiments.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

  • @ref: 2385
  • history: <- CSIC <- K.N. Timmis

doi: 10.13145/bacdive12896.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 2385

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

strain designation: mt-2 KT2440

type strain: no

Culture and growth conditions

culture medium

  • @ref: 2385
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 2385
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
2385---+----+-+---++-++++
2385---+----+-+---++-++++

Isolation, sampling and environmental information

isolation

  • @ref: 60908
  • sample type: Safety host for recombinant DNA experiments(r-,m+)

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Laboratory

External links

@ref: 2385

culture collection no.: DSM 6125, ATCC 47054, CCUG 56301, CFBP 8728

straininfo link

  • @ref: 82109
  • straininfo: 42206

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism23997333Biodegradation of Low Density Polythene (LDPE) by Pseudomonas Species.Kyaw BM, Champakalakshmi R, Sakharkar MK, Lim CS, Sakharkar KRIndian J Microbiol10.1007/s12088-012-0250-62012
29312257Cell-Surface Displayed Expression of Trehalose Synthase from Pseudomonas putida ATCC 47054 in Pichia Pastoris Using Pir1p as an Anchor Protein.Yang S, Lv X, Wang X, Wang J, Wang R, Wang TFront Microbiol10.3389/fmicb.2017.025832017
Enzymology29602391A process for production of trehalose by recombinant trehalose synthase and its purification.Liu H, Yang S, Liu Q, Wang R, Wang TEnzyme Microb Technol10.1016/j.enzmictec.2017.11.0082017Biocatalysis, Escherichia coli/*enzymology/genetics/growth & development, Fermentation, Glucosyltransferases/genetics/*metabolism, Maltose/metabolism, Pseudomonas putida/*enzymology/genetics/growth & development, Recombinant Proteins/genetics/*metabolism, Trehalose/*isolation & purification/*metabolismMetabolism
Metabolism32410635Valorization of Gelidium amansii for dual production of D-galactonic acid and 5-hydroxymethyl-2-furancarboxylic acid by chemo-biological approach.Liu P, Xie J, Tan H, Zhou F, Zou L, Ouyang JMicrob Cell Fact10.1186/s12934-020-01357-62020Acids/metabolism, *Biomass, Fermentation, Furaldehyde/*analogs & derivatives/metabolism, Galactose/metabolism, Hydrolysis, Oxidation-Reduction, Pseudomonas putida/metabolism, Rhodophyta/*chemistry, Sugar Acids/*metabolismBiotechnology
34300981Evaluation of 3D-Printing Scaffold Fabrication on Biosynthetic Medium-Chain-Length Polyhydroxyalkanoate Terpolyester as Biomaterial-Ink.Panaksri A, Tanadchangsaeng NPolymers (Basel)10.3390/polym131422222021

Reference

@idauthorscataloguedoi/urltitle
2385Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6125)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6125
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
60908Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56301)https://www.ccug.se/strain?id=56301
68369Automatically annotated from API 20NE
82109Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42206.1StrainInfo: A central database for resolving microbial strain identifiers