Name: Pseudomonas putida DSM 84
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12895

Type strain:

Species: Pseudomonas putida

Culture col. no.: DSM 84, IMG 1513

Strain history: <- IMG 1513 (Pseudomonas fluorescens) <- D. Claus

NCBI tax ID(s): 303 (species)

SI-ID 47054

CECT 4584, BCRC 15537, CCRC 15537, DSM 84

Special Collections

DSMZ

Other strains from Pseudomonas putida

Section

Pseudomonas putida DSM 84 is a human pathogen that was isolated from sewage.

human pathogen
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas putida**
	Full Scientific Name (LPSN)	Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

	Synonym	"Bacillus* putidus"*
	Synonym	Arthrobacter siderocapsulatus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2060]

Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

[Ref.: #2060]

Culture medium growth

[Ref.: #2060]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2060]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #2060]			growth	26 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #2060]

Name of produced compound

hydantoinase

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2060]	22800	-	-	-	+	-	-	-	-	+	-	+	-	-	-	+	+	-	+	+	+	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2060]	Sample type/isolated from	sewage
[Ref.: #2060]	Geographic location (country and/or sea, region)	Göttingen
[Ref.: #2060]	Country	Germany
[Ref.: #2060]	Country ISO 3 Code	DEU
[Ref.: #2060]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Wastewater

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2060]	Pathogenicity (human)	yes
[Ref.: #2060]	Pathogenicity (animal)	yes
[Ref.: #2060]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Availability in culture collections External links

[Ref.: #2060]

Culture collection no.

DSM 84, IMG 1513

[Ref.: #82108]

SI-ID 47054

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Crystal structures of lysine-preferred racemases, the non-antibiotic selectable markers for transgenic plants.	Wu HM, Kuan YC, Chu CH, Hsu WH, Wang WC	PLoS One	10.1371/journal.pone.0048301	2012	*
Transcriptome	Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli.	Chien HR, Jih YL, Yang WY, Hsu WH	Biochim Biophys Acta	10.1016/s0167-4781(97)00097-3	1998	*
Enzymology	Use of a polymerase-chain-reaction-amplified DNA probe from Pseudomonas putida to detect D-hydantoinase-producing microorganisms by direct colony hybridization.	LaPointe G, Leblanc D, Morin A	Appl Microbiol Biotechnol	10.1007/BF00191188	1995	*
Enzymology	Cloning, sequencing, and expression in Escherichia coli of the D-hydantoinase gene from Pseudomonas putida and distribution of homologous genes in other microorganisms.	LaPointe G, Viau S, LeBlanc D, Robert N, Morin A	Appl Environ Microbiol	10.1128/aem.60.3.888-895.1994	1994	*

References

#2060

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 84

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#68369

Automatically annotated from API 20NE .

#82108

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47054.1 )

* These data were automatically processed and therefore are not curated

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