Strain identifier

BacDive ID: 12895

Type strain: No

Species: Pseudomonas putida

Strain history: <- IMG 1513 (Pseudomonas fluorescens) <- D. Claus

NCBI tax ID(s): 303 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2060

BacDive-ID: 12895

DSM-Number: 84

keywords: Bacteria, mesophilic, human pathogen

description: Pseudomonas putida DSM 84 is a mesophilic human pathogen that was isolated from sewage.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

  • @ref: 2060
  • history: <- IMG 1513 (Pseudomonas fluorescens) <- D. Claus

doi: 10.13145/bacdive12895.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 2060

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

type strain: no

Culture and growth conditions

culture medium

  • @ref: 2060
  • name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1a
  • composition: Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 2060
  • growth: positive
  • type: growth
  • temperature: 26
  • range: mesophilic

Physiology and metabolism

compound production

  • @ref: 2060
  • compound: hydantoinase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
2060---+----+-+---++-++++/-

Isolation, sampling and environmental information

isolation

  • @ref: 2060
  • sample type: sewage
  • geographic location: Göttingen
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Wastewater

Safety information

risk assessment

  • @ref: 2060
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 2060

culture collection no.: DSM 84, IMG 1513

straininfo link

  • @ref: 82108
  • straininfo: 47054

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology7766091Use of a polymerase-chain-reaction-amplified DNA probe from Pseudomonas putida to detect D-hydantoinase-producing microorganisms by direct colony hybridization.LaPointe G, Leblanc D, Morin AAppl Microbiol Biotechnol10.1007/BF001911881995Amidohydrolases/*biosynthesis/*genetics, Bacteria/enzymology/genetics, Base Sequence, Biodegradation, Environmental, DNA Probes/*genetics, DNA, Bacterial/genetics, Genes, Bacterial, Hydantoins/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, Pseudomonadaceae/enzymology/genetics, Pseudomonas putida/*enzymology/*genetics, Rhizobium/enzymology/genetics, Sequence Homology, Nucleic AcidGenetics
Enzymology8161181Cloning, sequencing, and expression in Escherichia coli of the D-hydantoinase gene from Pseudomonas putida and distribution of homologous genes in other microorganisms.LaPointe G, Viau S, LeBlanc D, Robert N, Morin AAppl Environ Microbiol10.1128/aem.60.3.888-895.19941994Amidohydrolases/biosynthesis/*genetics, Amino Acid Sequence, Base Sequence, DNA Probes, Escherichia coli/*enzymology/genetics, Gene Expression Regulation, Enzymologic/genetics, Molecular Sequence Data, Open Reading Frames/*genetics, Pseudomonas putida/*enzymology/genetics, Sequence HomologyGenetics
Transcriptome9434154Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli.Chien HR, Jih YL, Yang WY, Hsu WHBiochim Biophys Acta10.1016/s0167-4781(97)00097-31998Amidohydrolases/chemistry/*genetics/metabolism, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli/genetics, Gene Expression Regulation, Bacterial/drug effects/*physiology, Genes, Bacterial/genetics, Lactose/pharmacology, Molecular Sequence Data, Molecular Weight, Open Reading Frames/*genetics, Pseudomonas putida/enzymology/*genetics/growth & development, Recombinant Fusion Proteins, Sequence Analysis, Sequence Analysis, DNA, Sequence Deletion, TemperatureEnzymology
Enzymology23118975Crystal structures of lysine-preferred racemases, the non-antibiotic selectable markers for transgenic plants.Wu HM, Kuan YC, Chu CH, Hsu WH, Wang WCPLoS One10.1371/journal.pone.00483012012Amino Acid Isomerases/*chemistry/genetics/*metabolism, Amino Acid Sequence, Biomarkers/chemistry/metabolism, Catalytic Domain, Crystallography, X-Ray, Kinetics, Lysine/*metabolism, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Plants, Genetically Modified, Proteus/*enzymology, Pseudomonas putida/*enzymology, Substrate SpecificityMetabolism

Reference

@idauthorscataloguedoi/urltitle
2060Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 84)https://www.dsmz.de/collection/catalogue/details/culture/DSM-84
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE
82108Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47054.1StrainInfo: A central database for resolving microbial strain identifiers