Strain identifier
BacDive ID: 12895
Type strain:
Species: Pseudomonas putida
Strain history: <- IMG 1513 (Pseudomonas fluorescens) <- D. Claus
NCBI tax ID(s): 303 (species)
version 8.1 (current version)
General
@ref: 2060
BacDive-ID: 12895
DSM-Number: 84
keywords: Bacteria, mesophilic, human pathogen
description: Pseudomonas putida DSM 84 is a mesophilic human pathogen that was isolated from sewage.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
- @ref: 2060
- history: <- IMG 1513 (Pseudomonas fluorescens) <- D. Claus
doi: 10.13145/bacdive12895.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 2060
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
type strain: no
Culture and growth conditions
culture medium
- @ref: 2060
- name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1a
- composition: Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
- @ref: 2060
- growth: positive
- type: growth
- temperature: 26
- range: mesophilic
Physiology and metabolism
compound production
- @ref: 2060
- compound: hydantoinase
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2060 | - | - | - | + | - | - | - | - | + | - | + | - | - | - | + | + | - | + | + | + | +/- |
Isolation, sampling and environmental information
isolation
- @ref: 2060
- sample type: sewage
- geographic location: Göttingen
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Wastewater
Safety information
risk assessment
- @ref: 2060
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 2060
culture collection no.: DSM 84, IMG 1513
straininfo link
- @ref: 82108
- straininfo: 47054
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Enzymology | 7766091 | Use of a polymerase-chain-reaction-amplified DNA probe from Pseudomonas putida to detect D-hydantoinase-producing microorganisms by direct colony hybridization. | LaPointe G, Leblanc D, Morin A | Appl Microbiol Biotechnol | 10.1007/BF00191188 | 1995 | Amidohydrolases/*biosynthesis/*genetics, Bacteria/enzymology/genetics, Base Sequence, Biodegradation, Environmental, DNA Probes/*genetics, DNA, Bacterial/genetics, Genes, Bacterial, Hydantoins/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, Pseudomonadaceae/enzymology/genetics, Pseudomonas putida/*enzymology/*genetics, Rhizobium/enzymology/genetics, Sequence Homology, Nucleic Acid | Genetics |
Enzymology | 8161181 | Cloning, sequencing, and expression in Escherichia coli of the D-hydantoinase gene from Pseudomonas putida and distribution of homologous genes in other microorganisms. | LaPointe G, Viau S, LeBlanc D, Robert N, Morin A | Appl Environ Microbiol | 10.1128/aem.60.3.888-895.1994 | 1994 | Amidohydrolases/biosynthesis/*genetics, Amino Acid Sequence, Base Sequence, DNA Probes, Escherichia coli/*enzymology/genetics, Gene Expression Regulation, Enzymologic/genetics, Molecular Sequence Data, Open Reading Frames/*genetics, Pseudomonas putida/*enzymology/genetics, Sequence Homology | Genetics |
Transcriptome | 9434154 | Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli. | Chien HR, Jih YL, Yang WY, Hsu WH | Biochim Biophys Acta | 10.1016/s0167-4781(97)00097-3 | 1998 | Amidohydrolases/chemistry/*genetics/metabolism, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli/genetics, Gene Expression Regulation, Bacterial/drug effects/*physiology, Genes, Bacterial/genetics, Lactose/pharmacology, Molecular Sequence Data, Molecular Weight, Open Reading Frames/*genetics, Pseudomonas putida/enzymology/*genetics/growth & development, Recombinant Fusion Proteins, Sequence Analysis, Sequence Analysis, DNA, Sequence Deletion, Temperature | Enzymology |
Enzymology | 23118975 | Crystal structures of lysine-preferred racemases, the non-antibiotic selectable markers for transgenic plants. | Wu HM, Kuan YC, Chu CH, Hsu WH, Wang WC | PLoS One | 10.1371/journal.pone.0048301 | 2012 | Amino Acid Isomerases/*chemistry/genetics/*metabolism, Amino Acid Sequence, Biomarkers/chemistry/metabolism, Catalytic Domain, Crystallography, X-Ray, Kinetics, Lysine/*metabolism, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Plants, Genetically Modified, Proteus/*enzymology, Pseudomonas putida/*enzymology, Substrate Specificity | Metabolism |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
2060 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 84) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-84 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
68369 | Automatically annotated from API 20NE | |||
82108 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47054.1 | StrainInfo: A central database for resolving microbial strain identifiers |