Strain identifier

BacDive ID: 135611

Type strain: No

Species: Pseudomonas putida

Strain history: CIP <- 1959, NCIB, Pseudomonas fluorescens <- J. Peel, Sheffield Univ., UK

NCBI tax ID(s): 303 (species)

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General

@ref: 34785

BacDive-ID: 135611

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas putida CIP 59.25 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from Soil.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

@refhistory
67770K. Komagata <-- R. Hugh 1189 <-- ATCC 12842 <-- NCIMB 8865 <-- J. L. Peel CO1.
34785CIP <- 1959, NCIB, Pseudomonas fluorescens <- J. Peel, Sheffield Univ., UK

doi: 10.13145/bacdive135611.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 34785

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

type strain: no

Morphology

cell morphology

  • @ref: 34785
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 34785
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34785MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
34785CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
34785CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
34785positivegrowth30mesophilic
67770positivegrowth30mesophilic
34785positivegrowth5-37
34785nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 34785
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
34785NaClpositivegrowth0-8 %
34785NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3478516947citrate+carbon source
347854853esculin-hydrolysis
3478517632nitrate-reduction
3478516301nitrite-reduction
3478517632nitrate-respiration

antibiotic resistance

  • @ref: 34785
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 34785
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
34785oxidase+
34785beta-galactosidase-3.2.1.23
34785alcohol dehydrogenase+1.1.1.1
34785gelatinase-
34785amylase-
34785DNase-
34785caseinase-3.4.21.50
34785catalase+1.11.1.6
34785tween esterase+
34785lecithinase-
34785lipase-
34785lysine decarboxylase-4.1.1.18
34785ornithine decarboxylase-4.1.1.17
34785protease-
34785tryptophan deaminase-
34785urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34785-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
34785+++-+--------------++----------+-----------++++++++++++++---+++++--+-+++++++++++++++++-++++++++++++

Isolation, sampling and environmental information

isolation

@refsample type
67770Soil
34785Environment, Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_239.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_203;99_239&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: LC507957
  • Sequence Identity:
  • Total samples: 2133
  • soil counts: 368
  • aquatic counts: 543
  • animal counts: 799
  • plant counts: 423

Safety information

risk assessment

  • @ref: 34785
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Pseudomonas fluorescens JCM 13060 gene for 16S rRNA, partial sequence
  • accession: LC507957
  • length: 1459
  • database: ena
  • NCBI tax ID: 294

External links

@ref: 34785

culture collection no.: CIP 59.25, ATCC 12842, NCIMB 8865, JCM 13060, CECT 385

straininfo link

  • @ref: 92935
  • straininfo: 35675

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34785Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2059.25Collection of Institut Pasteur (CIP 59.25)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
92935Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID35675.1