Name: Pseudomonas aeruginosa DSM 3227
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12764

Type strain:

Species: Pseudomonas aeruginosa

Culture col. no.: DSM 3227, ATCC 19429, IFO 3755, NBRC 3755, NCIB 6750, NCTC 6750

Strain history: <- NCTC <- H.L. Schutze

NCBI tax ID(s): 287 (species)

Other strains from Pseudomonas aeruginosa

Section

Pseudomonas aeruginosa DSM 3227 is a mesophilic human pathogen that has multiple antibiotic resistances and was isolated from urine.

antibiotic resistance
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas aeruginosa**
	Full Scientific Name (LPSN)	Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

Synonym

"Bacterium aeruginosum"

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.99

[Ref.: #69480]	Motility	yes Confidence in %97.291

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1322]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #1322]

Culture medium growth

[Ref.: #1322]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1322]

Culture medium composition

expand / minimize

[Ref.: #37963]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #37963]

Culture medium growth

[Ref.: #37963]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1322]			growth	37 °C
[Ref.: #37963]			growth	30 °C

[Ref.: #1322]	Temperature range	mesophilic
[Ref.: #37963]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Reference

[Ref.: #1322]
Mueller-Hinton Agar
37
1
aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
resistant0
24-26
14
resistant0
resistant0
18
24
28
12
10-12
20
24
resistant0
10
resistant0
20-22
30
14
16
resistant0
resistant0
16
12
16-18
10
22
resistant0
34
20
resistant0
resistant0
resistant0
resistant0
24

Only first 15 entries are displayed. Click here to see all.

[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.951

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #1322]	20360	+	-	-	+	+	-	+	-	+	-	-	+	+	+	+	+	+	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1322]	Sample type/isolated from	urine
* marker position based on {}

Isolation sources categories

#Host Body Product

#Fluids

#Urine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB680129 (>99% sequence identity) for Pseudomonas aeruginosa from Microbeatlas

Aquatic Samples	5046
Soil Samples	1669
Animal Samples	14748
Plant Samples	1294
Total Samples	22757

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1322]	Pathogenicity (human)	yes
[Ref.: #1322]	Pathogenicity (animal)	yes
[Ref.: #1322]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Pseudomonas aeruginosa gene for 16S rRNA, partial sequence, strain: NBRC 3755	AB680129	1461	ENA	287 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas aeruginosa NCTC6750	GCA_900461635	contig	GenBank	287 tax ID	*
[Ref.: #66792]	Pseudomonas aeruginosa strain NCTC6750	287.6523	wgs	PATRIC	287 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas aeruginosa strain NCTC6750 PATRIC: 287.6523
[Ref.: #69480]	anaerobic	no	98.246	no
[Ref.: #69480]	gram-positive	no	99.99	no
[Ref.: #69480]	spore-forming	no	99.951	no
[Ref.: #69480]	thermophile	no	99.898	yes
[Ref.: #69480]	motile	yes	97.291	yes

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudomonas aeruginosa strain NCTC6750 PATRIC: 287.6523
[Ref.: #69481]	spore-forming	no	99	no

Availability in culture collections External links

[Ref.: #1322]

Culture collection no.

DSM 3227, ATCC 19429, IFO 3755, NBRC 3755, NCIB 6750, NCTC 6750

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Antimicrobial activity of Mallotus oppositifolium extractives.	Ogundipe OO, Moody JO, Fakeye TO, Ladipo OB	Afr J Med Med Sci		2000	*
Enzymology	15N NMR spectroscopy of Pseudomonas cytochrome c-551.	Timkovich R	Biochemistry	10.1021/bi00485a029	1990	*
Enzymology	NMR comparison of prokaryotic and eukaryotic cytochromes c.	Chau MH, Cai ML, Timkovich R	Biochemistry	10.1021/bi00473a012	1990	*
Enzymology	Inactivation of cytochrome cd1 by hydrazines.	Yap-Bondoc F, Timkovich R	J Biol Chem	S0021-9258(19)39554-7	1990	*
Enzymology	Outer membrane proteins of gentamicin induced small colony variants of Pseudomonas aeruginosa.	Langford PR, Anwar H, Gonda I, Brown MR	FEMS Microbiol Lett	10.1016/0378-1097(89)90165-1	1989	*
Enzymology	1H NMR spectroscopy of cytochrome cd1 derivatives.	Timkovich R, Cork MS, Taylor PV	Arch Biochem Biophys	10.1016/0003-9861(85)90077-3	1985	*
Enzymology	Patterns of product inhibition for bacterial nitrite reductase.	Dhesi R, Timkovich R	Biochem Biophys Res Commun	10.1016/s0006-291x(84)80228-4	1984	*
Enzymology	Proposed structure for the noncovalently associated heme prosthetic group of dissimilatory nitrite reductases. Identification of substituents.	Timkovich R, Cork MS, Taylor PV	J Biol Chem	S0021-9258(17)43448-X	1984	*
Pathogenicity	Additivity of action between polysorbate 80 and polymyxin B towards spheroplasts of Pseudomonas aeruginosa NCTC 6750.	Brown MR, Geaton EM, Gilbert P	J Pharm Pharmacol	10.1111/j.2042-7158.1979.tb13463.x	1979	*
Pathogenicity	Influence of suspending media upon the susceptibility of Pseudomonas aeruginosa NCTC 6750 and its spheroplasts to polymyxin B.	Klemperer RM, Gilbert P, Meier AM, Cozens RM, Brown MR	Antimicrob Agents Chemother	10.1128/AAC.15.2.147	1979	*
Pathogenicity	Effect of ethylenediaminetetraacetic acid and related chelating agents on whole cells of gram-negative bacteria.	Haque H, Russell AD	Antimicrob Agents Chemother	10.1128/AAC.5.5.447	1974	*

References

#1322

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3227

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37963 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Pseudomonas aeruginosa strain NCTC6750

Pseudomonas aeruginosa strain NCTC6750

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