Pseudomonas aeruginosa (DSM 19882, PA14)
Pseudomonas aeruginosa PA14 is a human pathogen that has multiple antibiotic resistances.
antibiotic resistance human pathogen genome sequence BacteriaName and taxonomic classification
| @ref 20215 |
Last LPSN update
2026-05-29 08:27:53 |
| Domain Bacteria |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Pseudomonadales |
| Family Pseudomonadaceae |
| Genus Pseudomonas |
| Species Pseudomonas aeruginosa |
| Full scientific name Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980) |
| Synonyms (2) |
Culture and growth conditions
Culture medium
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 8365 | NUTRIENT AGAR (DSMZ Medium 1) | Medium recipe at MediaDive  | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
Culture temp
| @ref | Growth | Type | Temperature (°C) | |
|---|---|---|---|---|
| 8365 | positive | growth | 37 |
Physiology and metabolism
Antibiotic susceptibility testing
| Test 1 | |
|---|---|
| @ref | 8365 |
| Medium | Mueller-Hinton Agar |
| Manual annotation | 1 |
| Inhibition zone diameter in mm | |
| Amikacin 30µg (disc) | 30-32 |
| Ampicillin 10µg (disc) | 0 |
| Aztreonam 30µg (disc) | 30 |
| Bacitracin 10Unit | 0 |
| Cefalotin 30µg (disc) | 0 |
| Cefazolin 30µg (disc) | 0 |
| Cefotaxime 30µg (disc) | 22 |
| Ceftriaxone 30µg (disc) | 24 |
| Chloramphenicol 30µg (disc) | 14-16 |
| Clindamycin 10µg (disc) | 0 |
| Colistin 10µg (disc) | 14-16 |
| Doxycycline 30µg (disc) | 16 |
| Erythromycin 15µg (disc) | 10 |
| Fosfomycin 50µg (disc) | 28 |
| Gentamicin 10µg (disc) | 22 |
| Imipenem 10µg (disc) | 38 |
| Kanamycin 30µg (disc) | 12 |
| Lincomycin 15µg (disc) | 0 |
| Linezolid 10µg (disc) | 0 |
| Mezlocillin 30µg (disc) | 20 |
| Moxifloxacin 5µg (disc) | 26 |
| Neomycin 30µg (disc) | 18 |
| Nitrofurantoin 100µg (disc) | 0 |
| Norfloxacin 10µg (disc) | 34 |
| Nystatin 100Unit | 0 |
| Ofloxacin 5µg (disc) | 26-28 |
| Oxacillin 5µg (disc) | 0 |
| Penicillin G 6µg (disc) | 0 |
| Pipemidic acid 20µg (disc) | 16-18 |
| Piperacillin/Tazobactam 40µg (disc) | 30 |
| Polymyxin B 300Unit | 16 |
| Quinupristin/Dalfopristin 15µg (disc) | 0 |
| Teicoplanin 30µg (disc) | 0 |
| Tetracycline 30µg (disc) | 20 |
| Ticarcillin 75µg (disc) | 28 |
| Vancomycin 30µg (disc) | 0 |
Spore formation
| @ref | Spore formation | Confidence | |
|---|---|---|---|
| 125439 | 98.579 |
Metabolite utilization
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68369 | 17128 ChEBI | adipate | + | assimilation | from API 20NE |
| 68369 | 29016 ChEBI | arginine | + | hydrolysis | from API 20NE |
| 68369 | 17634 ChEBI | D-glucose | + | assimilation | from API 20NE |
| 68369 | 17634 ChEBI | D-glucose | - | fermentation | from API 20NE |
| 68369 | 16899 ChEBI | D-mannitol | + | assimilation | from API 20NE |
| 68369 | 16024 ChEBI | D-mannose | - | assimilation | from API 20NE |
| 68369 | 27689 ChEBI | decanoate | + | assimilation | from API 20NE |
| 68369 | 4853 ChEBI | esculin | - | hydrolysis | from API 20NE |
| 68369 | 5291 ChEBI | gelatin | + | hydrolysis | from API 20NE |
| 68369 | 24265 ChEBI | gluconate | + | assimilation | from API 20NE |
| 68369 | 30849 ChEBI | L-arabinose | - | assimilation | from API 20NE |
| 68369 | 25115 ChEBI | malate | + | assimilation | from API 20NE |
| 68369 | 17306 ChEBI | maltose | - | assimilation | from API 20NE |
| 68369 | 59640 ChEBI | N-acetylglucosamine | + | assimilation | from API 20NE |
| 68369 | 17632 ChEBI | nitrate | - | reduction | from API 20NE |
| 68369 | 27897 ChEBI | tryptophan | - | energy source | from API 20NE |
| 68369 | 16199 ChEBI | urea | - | hydrolysis | from API 20NE |
Antibiotic resistance
| @ref | ChEBI | Metabolite | Is resistant | Resistance conc. | |
|---|---|---|---|---|---|
| 8365 | 28971 | Ampicillin | 10 µg (disc) | from Antibiotic test | |
| 8365 | 28669 | Bacitracin | 10 Unit | from Antibiotic test | |
| 8365 | 124991 | Cefalotin | 30 µg (disc) | from Antibiotic test | |
| 8365 | 474053 | Cefazolin | 30 µg (disc) | from Antibiotic test | |
| 8365 | 3745 | Clindamycin | 10 µg (disc) | from Antibiotic test | |
| 8365 | 6472 | Lincomycin | 15 µg (disc) | from Antibiotic test | |
| 8365 | 63607 | Linezolid | 10 µg (disc) | from Antibiotic test | |
| 8365 | 71415 | Nitrofurantoin | 100 µg (disc) | from Antibiotic test | |
| 8365 | 7660 | Nystatin | 100 Unit | from Antibiotic test | |
| 8365 | 7809 | Oxacillin | 5 µg (disc) | from Antibiotic test | |
| 8365 | 18208 | Penicillin G | 6 µg (disc) | from Antibiotic test | |
| 8365 | Quinupristin/Dalfopristin | 15 µg (disc) | from Antibiotic test | ||
| 8365 | 29687 | Teicoplanin | 30 µg (disc) | from Antibiotic test | |
| 8365 | 28001 | Vancomycin | 30 µg (disc) | from Antibiotic test |
Enzymes
Interaction and safety
Risk assessment
Phages
| @ref | Name | Strain number | |
|---|---|---|---|
| 124042 | Pseudomonas phage JG012 (Unassigned) | DSM 19882 | |
| 124042 | Pseudomonas phage JG014 (Unassigned) | DSM 19882 | |
| 124042 | Pseudomonas phage JG017 (Unassigned) | DSM 19882 | |
| 124042 | Pseudomonas phage JG018 (Unassigned) | DSM 19882 | |
| 124042 | Pseudomonas phage JG024 (Unassigned) | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-1 | DSM 19882 | |
| 124042 | Pseudomonas phage BD-19882-4 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-7 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-14 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-20 | DSM 19882 | |
| 124042 | Pseudomonas phage BD-19882-21 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-25 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-28 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-42 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-47 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-56 | DSM 19882 | |
| 124042 | Pseudomonas phage BD 19882-57 | DSM 19882 | |
| 124042 | Pseudomonas phage vB_PaeS-PT-MAS03 | DSM 19882 | |
| 124042 | Pseudomonas phage vB_PaeS-PT-MAS04 | DSM 19882 | |
| 124042 | Pseudomonas phage vB_PaeP-PT-MAS05 | DSM 19882 |
Sequence information
Genome sequences
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 124043 | 934 assembly for Pseudomonas aeruginosa DSM 19882 | complete | GCA_050569945   | 287 | 98.87 | | ||
| 66792 | ASM2549047v1 assembly for Pseudomonas aeruginosa PA14 | chromosome | GCA_025490475 | 652611.54  | 652611 | 87.47 | | |
| 66792 | ASM2549041v1 assembly for Pseudomonas aeruginosa PA14 | chromosome | GCA_025490415 | 652611.51 | 652611 | 87.32 | | |
| 66792 | ASM2549045v1 assembly for Pseudomonas aeruginosa PA14 | chromosome | GCA_025490455 | 652611.53 | 652611 | 87.17 | | |
| 66792 | ASM2549037v1 assembly for Pseudomonas aeruginosa PA14 | chromosome | GCA_025490375 | 652611.49 | 652611 | 87.07 | | |
| 66792 | ASM2549039v1 assembly for Pseudomonas aeruginosa PA14 | chromosome | GCA_025490395 | 652611.50 | 652611 | 86.87 | | |
| 66792 | ASM2549043v1 assembly for Pseudomonas aeruginosa PA14 | chromosome | GCA_025490435 | 652611.52 | 652611 | 86.57 | | |
| 66792 | Pseu_aeru_PA14_V1 assembly for Pseudomonas aeruginosa PA14 | scaffold | GCA_000404265 | 652611.13 | 2541047093  | 652611 | 74.53 | |
| 66792 | 201_Pa14_contigs_500 assembly for Pseudomonas aeruginosa | contig | GCA_900005845 | 2734482529 | 287 | 64.17 | |
Genome browser: GCA_050569945
Literature
Literature 
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Antimicrobial Potential of Bacteriophages JG005 and JG024 Against Pseudomonas aeruginosa Isolates from Canine Otitis. | Lourenco MR, Cunha E, Tavares L, Oliveira M. | Vet Sci | 10.3390/vetsci12070646 | 2025 | | |
| Bio-electrosynthesis of polyhydroxybutyrate and surfactants in microbial fuel cells: a preliminary study. | Nastro RA, Kuppam C, Toscanesi M, Trifuoggi M, Pietrelli A, Pasquale V, Avignone-Rossa C. | Front Microbiol | 10.3389/fmicb.2025.1372302 | 2025 | | |
| Systematic bacteriophage selection for the lysis of multiple Pseudomonas aeruginosa strains. | Rieper F, Wittmann J, Bunk B, Sproer C, Hafner M, Willy C, Musken M, Ziehr H, Korf IHE, Jahn D. | Front Cell Infect Microbiol | 10.3389/fcimb.2025.1597009 | 2025 | | |
| Relevance of the bacteriophage adherence to mucus model for Pseudomonas aeruginosa phages. | Almeida GMdF, Ravantti J, Grdzelishvili N, Kakabadze E, Bakuradze N, Javakhishvili E, Megremis S, Chanishvili N, Papadopoulos N, Sundberg L-R. | Microbiol Spectr | 10.1128/spectrum.03520-23 | 2024 | | |
| Inhibition of Pseudomonas aeruginosa quorum sensing by methyl gallate from Mangifera indica. | Naga NG, Zaki AA, El-Badan DE, Rateb HS, Ghanem KM, Shaaban MI. | Sci Rep | 10.1038/s41598-023-44063-0 | 2023 | | |
| Laser-assisted microbial culturomics. | Qu T, Koch L, Mukherjee R, Tu Y, Seidel AL, Puttmann LD, Winkel A, Yang I, Grischke J, Liu D, Wolkers WF, Kittler S, Chichkov B, Stiesch M, Szafranski SP. | Nat Commun | 10.1038/s41467-025-66804-7 | 2025 | | |
| Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4. | Nastro RA, Salvian A, Kuppam C, Pasquale V, Pietrelli A, Rossa CA. | Microorganisms | 10.3390/microorganisms11030735 | 2023 | | |
| New Genetically Engineered Derivatives of Antibacterial Darobactins Underpin Their Potential for Antibiotic Development. | Seyfert CE, Muller AV, Walsh DJ, Birkelbach J, Kany AM, Porten C, Yuan B, Krug D, Herrmann J, Marlovits TC, Hirsch AKH, Muller R. | J Med Chem | 10.1021/acs.jmedchem.3c01660 | 2023 | | |
| Bioactive Compounds from an Endophytic Pezicula sp. Showing Antagonistic Effects against the Ash Dieback Pathogen | Demir O, Zeng H, Schulz B, Schrey H, Steinert M, Stadler M, Surup F. | Biomolecules | 2023 | | ||
| Repetitive Exposure to Bacteriophage Cocktails against Pseudomonas aeruginosa or Escherichia coli Provokes Marginal Humoral Immunity in Naïve Mice. | Weissfuss C, Wienhold SM, Burkle M, Gaborieau B, Bushe J, Behrendt U, Bischoff R, Korf IHE, Wienecke S, Dannheim A, Ziehr H, Rohde C, Gruber AD, Ricard JD, Debarbieux L, Witzenrath M, Nouailles G. | Viruses | 10.3390/v15020387 | 2023 | | |
| Metabolism | Genome-Guided Discovery of the First Myxobacterial Biarylitide Myxarylin Reveals Distinct C-N Biaryl Crosslinking in RiPP Biosynthesis. | Hug JJ, Frank NA, Walt C, Senica P, Panter F, Muller R. | Molecules | 10.3390/molecules26247483 | 2021 | |
| Phenotypical Characteristics of the Black Yeast Exophiala dermatitidis Are Affected by Pseudomonas aeruginosa in an Artificial Sputum Medium Mimicking Cystic Fibrosis-Like Conditions. | Kirchhoff L, Weisner AK, Schrepffer M, Hain A, Scharmann U, Buer J, Rath PM, Steinmann J. | Front Microbiol | 10.3389/fmicb.2020.00471 | 2020 | | |
| ent-Clavilactone J and Its Quinone Derivative, Meroterpenoids from the Fungus Resupinatus sp. | Harms K, Paomephan P, Boonpratuang T, Choeyklin R, Boonchird C, Surup F. | J Nat Prod | 10.1021/acs.jnatprod.3c00174 | 2023 | | |
| Pathogenicity | Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains. | Gonzalez-Garcia M, Morales-Vicente F, Pico ED, Garay H, Rivera DG, Grieshober M, Raluca Olari L, Gross R, Conzelmann C, Kruger F, Zech F, Prelli Bozzo C, Muller JA, Zelikin A, Raber H, Kubiczek D, Rosenau F, Munch J, Stenger S, Spellerberg B, Franco OL, Rodriguez Alfonso AA, Standker L, Otero-Gonzalez AJ. | Biomolecules | 10.3390/biom11050745 | 2021 | |
| Targeting Bacterial Gyrase with Cystobactamid, Fluoroquinolone, and Aminocoumarin Antibiotics Induces Distinct Molecular Signatures in Pseudomonas aeruginosa. | Franke R, Overwin H, Haussler S, Bronstrup M. | mSystems | 10.1128/msystems.00610-21 | 2021 | | |
| Structure Elucidation and Biosynthesis of Nannosterols A and B, Myxobacterial Sterols from Nannocystis sp. MNa10993. | Akone SH, Hug JJ, Kaur A, Garcia R, Muller R. | J Nat Prod | 10.1021/acs.jnatprod.2c01143 | 2023 | | |
| Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence. | Pira H, Risdian C, Musken M, Schupp PJ, Wink J. | Curr Microbiol | 10.1007/s00284-022-02909-2 | 2022 | | |
| Three New Stigmatellin Derivatives Reveal Biosynthetic Insights of Its Side Chain Decoration. | Okoth DA, Hug JJ, Garcia R, Muller R. | Molecules | 10.3390/molecules27144656 | 2022 | | |
| Pathogenicity | Synthesis and Bioactivity of Ancorinoside B, a Marine Diglycosyl Tetramic Acid. | Soliga KJ, Bar SI, Oberhuber N, Zeng H, Schrey H, Schobert R. | Mar Drugs | 10.3390/md19100583 | 2021 | |
| Lectin-Targeted Prodrugs Activated by Pseudomonas aeruginosa for Self-Destructive Antibiotic Release. | Meiers J, Rox K, Titz A. | J Med Chem | 10.1021/acs.jmedchem.2c01214 | 2022 | | |
| Pathogenicity | Directing Drugs to Bugs: Antibiotic-Carbohydrate Conjugates Targeting Biofilm-Associated Lectins of Pseudomonas aeruginosa. | Meiers J, Zahorska E, Rohrig T, Hauck D, Wagner S, Titz A. | J Med Chem | 10.1021/acs.jmedchem.0c00856 | 2020 | |
| Corallococcus soli sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary Metabolites. | Babadi ZK, Garcia R, Ebrahimipour GH, Risdian C, Kampfer P, Jarek M, Muller R, Wink J. | Microorganisms | 10.3390/microorganisms10071262 | 2022 | | |
| Morinagadepsin, a Depsipeptide from the Fungus Morinagamyces vermicularis gen. et comb. nov. | Harms K, Surup F, Stadler M, Stchigel AM, Marin-Felix Y. | Microorganisms | 10.3390/microorganisms9061191 | 2021 | | |
| Streptomonospora litoralis sp. nov., a halophilic thiopeptides producer isolated from sand collected at Cuxhaven beach. | Khodamoradi S, Hahnke RL, Mast Y, Schumann P, Kampfer P, Steinert M, Ruckert C, Surup F, Rohde M, Wink J. | Antonie Van Leeuwenhoek | 10.1007/s10482-021-01609-4 | 2021 | |
How to cite
If you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive12796.20260601.11
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain dataIsabel Schober, Julia Koblitz, Joaquim Sardà Carbasse, Christian Ebeling, Marvin Leon Schmidt, Adam Podstawka, Rohit Gupta, Vinodh Ilangovan, Javad Chamanara, Jörg Overmann, Lorenz Christian Reimer
Nucleic Acids Research; database issue 2025.
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