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Strain identifier

BacDive ID: 12796

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: PA14

Strain history: <- M. Schobert, Technical University Braunschweig < F. M. Ausubel, Massachusetts General Hospital, Boston

NCBI tax ID(s): 287 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8365

BacDive-ID: 12796

DSM-Number: 19882

keywords: genome sequence, Bacteria, mesophilic, antibiotic resistance, human pathogen

description: Pseudomonas aeruginosa PA14 is a mesophilic human pathogen that has multiple antibiotic resistances.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history: <- M. Schobert, Technical University Braunschweig < F. M. Ausubel, Massachusetts General Hospital, Boston

doi: 10.13145/bacdive12796.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 8365

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

strain designation: PA14

type strain: no

Culture and growth conditions

culture medium

  • @ref: 8365
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 8365
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

antibiogram

  • @ref: 8365
  • medium: Mueller-Hinton Agar
  • incubation temperature: 37
  • oxygen condition: aerob
  • Penicillin G: 0
  • oxacillin: 0
  • ampicillin: 0
  • ticarcillin: 28
  • mezlocillin: 20
  • cefalotin: 0
  • cefazolin: 0
  • cefotaxime: 22
  • aztreonam: 30
  • imipenem: 38
  • tetracycline: 20
  • chloramphenicol: 14-16
  • gentamycin: 22
  • amikacin: 30-32
  • vancomycin: 0
  • erythromycin: 10
  • lincomycin: 0
  • ofloxacin: 26-28
  • norfloxacin: 34
  • colistin: 14-16
  • pipemidic acid: 16-18
  • nitrofurantoin: 0
  • bacitracin: 0
  • polymyxin b: 16
  • kanamycin: 12
  • neomycin: 18
  • doxycycline: 16
  • ceftriaxone: 24
  • clindamycin: 0
  • fosfomycin: 28
  • moxifloxacin: 26
  • linezolid: 0
  • nystatin: 0
  • quinupristin/dalfopristin: 0
  • teicoplanin: 0
  • piperacillin/tazobactam: 30

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
8365---+--+-+--++-+++++-+

Safety information

risk assessment

  • @ref: 8365
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas aeruginosa PA14GCA_900005845contigncbi287
66792Pseudomonas aeruginosa PA14 PA14GCA_000404265scaffoldncbi652611
66792Pseudomonas aeruginosa PA14652611.13wgspatric652611
66792Pseudomonas aeruginosa PA142541047093draftimg652611
66792Pseudomonas aeruginosa PA14 PA14GCA_025490375chromosomencbi652611
66792Pseudomonas aeruginosa PA14 PA14GCA_025490395chromosomencbi652611
66792Pseudomonas aeruginosa PA14 PA14GCA_025490415chromosomencbi652611
66792Pseudomonas aeruginosa PA14 PA14GCA_025490435chromosomencbi652611
66792Pseudomonas aeruginosa PA14 PA14GCA_025490455chromosomencbi652611
66792Pseudomonas aeruginosa PA14 PA14GCA_025490475chromosomencbi652611
66792Mycobacterium tuberculosis A70136GCA_001155985scaffoldpatric1773
66792Staphylococcus aureus CFSA113GCA_002124985scaffoldpatric1280
66792Salmonella enterica subsp. enterica serovar Enteritidis BCMOA-D1-TMB-34GCA_014121475contigpatric149539
66792Salmonella enterica subsp. enterica serovar Enteritidis BCMOA-D1-TMB-39GCA_014235885contigpatric149539
66792Staphylococcus aureus FMV16-5587GCA_019426145contigpatric1280
66792Klebsiella pneumoniae Kp178GCA_021395515contigpatric573
66792Klebsiella pneumoniae HK1027GCA_025754785contigpatric573

External links

@ref: 8365

culture collection no.: DSM 19882

Reference

@idauthorscataloguedoi/urltitle
8365Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19882)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19882
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE