Strain identifier

BacDive ID: 12796

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: PA14

Strain history: <- M. Schobert, Technical University Braunschweig < F. M. Ausubel, Massachusetts General Hospital, Boston

NCBI tax ID(s): 287 (species)

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General

@ref: 8365

BacDive-ID: 12796

DSM-Number: 19882

keywords: genome sequence, Bacteria, mesophilic, antibiotic resistance, human pathogen

description: Pseudomonas aeruginosa PA14 is a mesophilic human pathogen that has multiple antibiotic resistances.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 8365
  • history: <- M. Schobert, Technical University Braunschweig < F. M. Ausubel, Massachusetts General Hospital, Boston

doi: 10.13145/bacdive12796.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 8365

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

strain designation: PA14

type strain: no

Culture and growth conditions

culture medium

  • @ref: 8365
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 8365
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

antibiogram

  • @ref: 8365
  • medium: Mueller-Hinton Agar
  • incubation temperature: 37
  • oxygen condition: aerob
  • Penicillin G: 0
  • oxacillin: 0
  • ampicillin: 0
  • ticarcillin: 28
  • mezlocillin: 20
  • cefalotin: 0
  • cefazolin: 0
  • cefotaxime: 22
  • aztreonam: 30
  • imipenem: 38
  • tetracycline: 20
  • chloramphenicol: 14-16
  • gentamycin: 22
  • amikacin: 30-32
  • vancomycin: 0
  • erythromycin: 10
  • lincomycin: 0
  • ofloxacin: 26-28
  • norfloxacin: 34
  • colistin: 14-16
  • pipemidic acid: 16-18
  • nitrofurantoin: 0
  • bacitracin: 0
  • polymyxin b: 16
  • kanamycin: 12
  • neomycin: 18
  • doxycycline: 16
  • ceftriaxone: 24
  • clindamycin: 0
  • fosfomycin: 28
  • moxifloxacin: 26
  • linezolid: 0
  • nystatin: 0
  • quinupristin/dalfopristin: 0
  • teicoplanin: 0
  • piperacillin/tazobactam: 30

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
8365---+--+-+--++-+++++-+

Safety information

risk assessment

  • @ref: 8365
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas aeruginosa PA14GCA_900005845contigncbi287
66792Pseudomonas aeruginosa PA14GCA_000404265scaffoldncbi652611
66792Pseudomonas aeruginosa PA14652611.13wgspatric652611
66792Pseudomonas aeruginosa PA142541047093draftimg652611
66792Pseudomonas aeruginosa PA14GCA_025490375chromosomencbi652611
66792Pseudomonas aeruginosa PA14GCA_025490395chromosomencbi652611
66792Pseudomonas aeruginosa PA14GCA_025490415chromosomencbi652611
66792Pseudomonas aeruginosa PA14GCA_025490435chromosomencbi652611
66792Pseudomonas aeruginosa PA14GCA_025490455chromosomencbi652611
66792Pseudomonas aeruginosa PA14GCA_025490475chromosomencbi652611

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.177no
flagellatedyes86.246no
gram-positiveno98.511no
anaerobicno98.581no
aerobicyes93.8no
halophileno91.477no
spore-formingno93.257no
glucose-fermentno90.256no
thermophileno99.811no
glucose-utilyes89.371yes

External links

@ref: 8365

culture collection no.: DSM 19882

straininfo link

  • @ref: 82006
  • straininfo: 404498

Reference

@idauthorscataloguedoi/urltitle
8365Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19882)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19882
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
82006Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404498.1StrainInfo: A central database for resolving microbial strain identifiers