Pseudomonas aeruginosa (DSM 1707, PAO)

Multimedia

• @ref:	864
  multimedia content:	DSM_1707.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_1707.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
864	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

Compound production

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	urease	+	3.5.1.5	from API 20NE

API 20NE

Risk assessment

Literature

Topic	Title	Authors	Journal	DOI	Year
	High-Affinity Lectin Ligands Enable the Detection of Pathogenic Pseudomonas aeruginosa Biofilms: Implications for Diagnostics and Therapy.	Zahorska E, Denig LM, Lienenklaus S, Kuhaudomlarp S, Tschernig T, Lipp P, Munder A, Gillon E, Minervini S, Verkhova V, Imberty A, Wagner S, Titz A.	JACS Au	10.1021/jacsau.4c00670	2024
	Mucin adhesion of serial cystic fibrosis airways Pseudomonas aeruginosa isolates.	Herrmann C, Lingner M, Herrmann S, Brockhausen I, Tummler B.	Front Cell Infect Microbiol	10.3389/fcimb.2024.1448104	2024
	Antimicrobial Peptides Originating from Expression Libraries of Aurelia aurita and Mnemiopsis leidyi Prevent Biofilm Formation of Opportunistic Pathogens.	Ladewig L, Gloy L, Langfeldt D, Pinnow N, Weiland-Brauer N, Schmitz RA.	Microorganisms	10.3390/microorganisms11092184	2023
	Beyond Intracellular Accumulation of Polyhydroxyalkanoates: Chiral Hydroxyalkanoic Acids and Polymer Secretion.	Yanez L, Conejeros R, Vergara-Fernandez A, Scott F.	Front Bioeng Biotechnol	10.3389/fbioe.2020.00248	2020
	Intestinal tissues induce an SNP mutation in Pseudomonas aeruginosa that enhances its virulence: possible role in anastomotic leak.	Olivas AD, Shogan BD, Valuckaite V, Zaborin A, Belogortseva N, Musch M, Meyer F, Trimble WL, An G, Gilbert J, Zaborina O, Alverdy JC.	PLoS One	10.1371/journal.pone.0044326	2012
Metabolism	Isolation and characterization of Sphingomonadaceae from fouled membranes.	de Vries HJ, Beyer F, Jarzembowska M, Lipinska J, van den Brink P, Zwijnenburg A, Timmers PHA, Stams AJM, Plugge CM.	NPJ Biofilms Microbiomes	10.1038/s41522-018-0074-1	2019
Metabolism	High-frequency flp recombinase-mediated inversions of the oriC-containing region of the Pseudomonas aeruginosa genome.	Barekzi N, Beinlich K, Hoang TT, Pham XQ, Karkhoff-Schweizer R, Schweizer HP.	J Bacteriol	10.1128/jb.182.24.7070-7074.2000	2000
Genetics	Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome.	Dotsch A, Pommerenke C, Bredenbruch F, Geffers R, Haussler S.	BMC Genomics	10.1186/1471-2164-10-29	2009
Phenotype	Visualization and curve-parameter estimation strategies for efficient exploration of phenotype microarray kinetics.	Vaas LA, Sikorski J, Michael V, Goker M, Klenk HP.	PLoS One	10.1371/journal.pone.0034846	2012
	Post-Synthetic Enzymatic and Chemical Modifications for Novel Sustainable Polyesters.	El-Malek FA, Steinbuchel A.	Front Bioeng Biotechnol	10.3389/fbioe.2021.817023	2021
	Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains.	Klockgether J, Munder A, Neugebauer J, Davenport CF, Stanke F, Larbig KD, Heeb S, Schock U, Pohl TM, Wiehlmann L, Tummler B.	J Bacteriol	10.1128/jb.01515-09	2010
	Epidemiology of chronic Pseudomonas aeruginosa infections in the airways of lung transplant recipients with cystic fibrosis.	Walter S, Gudowius P, Bosshammer J, Romling U, Weissbrodt H, Schurmann W, von der Hardt H, Tummler B.	Thorax	10.1136/thx.52.4.318	1997
	Localization of alg, opr, phn, pho, 4.5S RNA, 6S RNA, tox, trp, and xcp genes, rrn operons, and the chromosomal origin on the physical genome map of Pseudomonas aeruginosa PAO.	Romling U, Duchene M, Essar DW, Galloway D, Guidi-Rontani C, Hill D, Lazdunski A, Miller RV, Schleifer KH, Smith DW.	J Bacteriol	10.1128/jb.174.1.327-330.1992	1992
Metabolism	Novel reporter for identification of interference with acyl homoserine lactone and autoinducer-2 quorum sensing.	Weiland-Brauer N, Pinnow N, Schmitz RA.	Appl Environ Microbiol	10.1128/aem.03290-14	2015
Pathogenicity	Quorum-sensing antagonistic activities of azithromycin in Pseudomonas aeruginosa PAO1: a global approach.	Nalca Y, Jansch L, Bredenbruch F, Geffers R, Buer J, Haussler S.	Antimicrob Agents Chemother	10.1128/aac.50.5.1680-1688.2006	2006
Phylogeny	Inter- and intraclonal diversity of the Pseudomonas aeruginosa proteome manifests within the secretome.	Wehmhoner D, Haussler S, Tummler B, Jansch L, Bredenbruch F, Wehland J, Steinmetz I.	J Bacteriol	10.1128/jb.185.19.5807-5814.2003	2003
Pathogenicity	Effects of antibiotics on quorum sensing in Pseudomonas aeruginosa.	Skindersoe ME, Alhede M, Phipps R, Yang L, Jensen PO, Rasmussen TB, Bjarnsholt T, Tolker-Nielsen T, Hoiby N, Givskov M.	Antimicrob Agents Chemother	10.1128/aac.01230-07	2008
Genetics	Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species.	Farrelly V, Rainey FA, Stackebrandt E.	Appl Environ Microbiol	10.1128/aem.61.7.2798-2801.1995	1995
Genetics	Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats.	Schmidt KD, Tummler B, Romling U.	J Bacteriol	10.1128/jb.178.1.85-93.1996	1996
Enzymology	Highly Effective Inhibition of Biofilm Formation by the First Metagenome-Derived AI-2 Quenching Enzyme.	Weiland-Brauer N, Kisch MJ, Pinnow N, Liese A, Schmitz RA.	Front Microbiol	10.3389/fmicb.2016.01098	2016
Phylogeny	Diversity of the abundant pKLC102/PAGI-2 family of genomic islands in Pseudomonas aeruginosa.	Klockgether J, Wurdemann D, Reva O, Wiehlmann L, Tummler B.	J Bacteriol	10.1128/jb.01688-06	2007
	Cloning and sequencing of the genes coding for the 10- and 60-kDa heat shock proteins from Pseudomonas aeruginosa and mapping of a species-specific epitope.	Sipos A, Klocke M, Frosch M.	Infect Immun	10.1128/iai.59.9.3219-3226.1991	1991
Metabolism	Formation of polyesters consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and other fluorescent pseudomonads.	Timm A, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.56.11.3360-3367.1990	1990
Metabolism	Role of the Tat ransport system in nitrous oxide reductase translocation and cytochrome cd1 biosynthesis in Pseudomonas stutzeri.	Heikkila MP, Honisch U, Wunsch P, Zumft WG.	J Bacteriol	10.1128/jb.183.5.1663-1671.2001	2001
Enzymology	Comparative mapping of the Pseudomonas aeruginosa PAO genome with rare-cutter linking clones or two-dimensional pulsed-field gel electrophoresis protocols.	Romling U, Tummler B	Electrophoresis	10.1002/elps.1150140150	1993
Enzymology	A PacI/SwaI map of the Pseudomonas aeruginosa PAO chromosome.	Romling U, Tummler B	Electrophoresis	10.1002/elps.11501301135	1992
Enzymology	The impact of two-dimensional pulsed-field gel electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO.	Romling U, Tummler B	Nucleic Acids Res	10.1093/nar/19.12.3199	1991
Enzymology	A physical genome map of Pseudomonas aeruginosa PAO.	Romling U, Grothues D, Bautsch W, Tummler B	EMBO J	10.1002/j.1460-2075.1989.tb08592.x	1989