Strain identifier
BacDive ID: 12764
Type strain:
Species: Pseudomonas aeruginosa
Strain history: CIP <- 1997, NCIMB <- NCTC <- H. Schutze
NCBI tax ID(s): 287 (species)
General
@ref: 1322
BacDive-ID: 12764
DSM-Number: 3227
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, motile, antibiotic resistance, human pathogen
description: Pseudomonas aeruginosa DSM 3227 is an obligate aerobe, mesophilic, motile human pathogen that has multiple antibiotic resistances and was isolated from urine.
NCBI tax id
- NCBI tax id: 287
- Matching level: species
strain history
@ref | history |
---|---|
1322 | <- NCTC <- H.L. Schutze |
116085 | CIP <- 1997, NCIMB <- NCTC <- H. Schutze |
doi: 10.13145/bacdive12764.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas aeruginosa
- full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacterium aeruginosum
@ref: 1322
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas aeruginosa
full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900
type strain: no
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | yes | 97.291 | ||
69480 | 99.99 | negative | ||
116085 | yes | negative | rod-shaped |
pigmentation
- @ref: 116085
- production: yes
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
1322 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
37963 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
116085 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1322 | positive | growth | 37 | mesophilic |
37963 | positive | growth | 30 | mesophilic |
116085 | positive | growth | 25-41 | |
116085 | no | growth | 5 | psychrophilic |
116085 | no | growth | 10 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 116085
- oxygen tolerance: obligate aerobe
antibiogram
- @ref: 1322
- medium: Mueller-Hinton Agar
- incubation temperature: 37
- incubation time: 1
- oxygen condition: aerob
- Penicillin G: 0
- oxacillin: 0
- ampicillin: 0
- ticarcillin: 24-26
- mezlocillin: 14
- cefalotin: 0
- cefazolin: 0
- cefotaxime: 18
- aztreonam: 24
- imipenem: 28
- tetracycline: 12
- chloramphenicol: 10-12
- gentamycin: 20
- amikacin: 24
- vancomycin: 0
- erythromycin: 10
- lincomycin: 0
- ofloxacin: 20-22
- norfloxacin: 30
- colistin: 14
- pipemidic acid: 16
- nitrofurantoin: 0
- bacitracin: 0
- polymyxin b: 16
- kanamycin: 12
- neomycin: 16-18
- doxycycline: 10
- ceftriaxone: 22
- clindamycin: 0
- fosfomycin: 34
- moxifloxacin: 20
- linezolid: 0
- nystatin: 0
- quinupristin/dalfopristin: 0
- teicoplanin: 0
- piperacillin/tazobactam: 24
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 99 |
69480 | no | 99.951 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
116085 | NaCl | positive | growth | 0-8 % |
116085 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | + | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | + | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | + | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | + | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
116085 | 16947 | citrate | + | carbon source |
116085 | 4853 | esculin | - | hydrolysis |
116085 | 17632 | nitrate | + | reduction |
116085 | 16301 | nitrite | + | reduction |
116085 | 15882 | phenol | + | degradation |
116085 | 17632 | nitrate | + | respiration |
antibiotic resistance
- @ref: 116085
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
116085 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | + | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | + | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
116085 | oxidase | + | |
116085 | beta-galactosidase | - | 3.2.1.23 |
116085 | alcohol dehydrogenase | + | 1.1.1.1 |
116085 | gelatinase | +/- | |
116085 | amylase | - | |
116085 | DNase | - | |
116085 | caseinase | + | 3.4.21.50 |
116085 | catalase | + | 1.11.1.6 |
116085 | tween esterase | - | |
116085 | lecithinase | - | |
116085 | lipase | - | |
116085 | lysine decarboxylase | - | 4.1.1.18 |
116085 | ornithine decarboxylase | - | 4.1.1.17 |
116085 | protease | + | |
116085 | tryptophan deaminase | - | |
116085 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116085 | - | - | + | + | + | + | - | - | + | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1322 | + | - | - | + | + | - | + | - | + | - | - | + | + | + | + | + | + | + | + | - | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116085 | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | - | - | - | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | + | - | - | + | - | - | + | + | - | + | + | + | + | - | + | - | - | - | + | + | + | + | + | + | + | + | + | + | + | + | + | + | - | - | + | + | + | + | + | + | + | + | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
1322 | urine |
116085 | Urine |
isolation source categories
- Cat1: #Host Body Product
- Cat2: #Fluids
- Cat3: #Urine
taxonmaps
- @ref: 69479
- File name: preview.99_42.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_36;97_37;98_40;99_42&stattab=map
- Last taxonomy: Pseudomonas aeruginosa
- 16S sequence: AB680129
- Sequence Identity:
- Total samples: 22757
- soil counts: 1669
- aquatic counts: 5046
- animal counts: 14748
- plant counts: 1294
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
1322 | yes | yes | 2 | Risk group (German classification) |
116085 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 20218
- description: Pseudomonas aeruginosa gene for 16S rRNA, partial sequence, strain: NBRC 3755
- accession: AB680129
- length: 1461
- database: ena
- NCBI tax ID: 287
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pseudomonas aeruginosa NCTC6750 | GCA_900461635 | contig | ncbi | 287 |
66792 | Pseudomonas aeruginosa strain NCTC6750 | 287.6523 | wgs | patric | 287 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 99 | no |
gram-positive | no | 98.4 | no |
anaerobic | no | 98.373 | no |
halophile | no | 90.888 | no |
spore-forming | no | 93.185 | no |
glucose-util | yes | 89.726 | no |
motile | yes | 88.741 | no |
flagellated | yes | 84.39 | no |
aerobic | yes | 93.275 | no |
thermophile | no | 99.792 | yes |
glucose-ferment | no | 90.21 | no |
External links
@ref: 1322
culture collection no.: DSM 3227, ATCC 19429, IFO 3755, NBRC 3755, NCIB 6750, NCTC 6750, CIP 105094, CCM 1959, IMET 10906, NCIMB 6750, CCEB 478
straininfo link
- @ref: 81973
- straininfo: 45626
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Pathogenicity | 34692 | Additivity of action between polysorbate 80 and polymyxin B towards spheroplasts of Pseudomonas aeruginosa NCTC 6750. | Brown MR, Geaton EM, Gilbert P | J Pharm Pharmacol | 10.1111/j.2042-7158.1979.tb13463.x | 1979 | Polyethylene Glycols/*pharmacology, Polymyxin B/*pharmacology, Polymyxins/*pharmacology, Polysorbates/*pharmacology, Pseudomonas aeruginosa/*drug effects/growth & development/ultrastructure, Spheroplasts/*drug effects, Time Factors | |
Pathogenicity | 218501 | Influence of suspending media upon the susceptibility of Pseudomonas aeruginosa NCTC 6750 and its spheroplasts to polymyxin B. | Klemperer RM, Gilbert P, Meier AM, Cozens RM, Brown MR | Antimicrob Agents Chemother | 10.1128/AAC.15.2.147 | 1979 | Bacillus megaterium/drug effects/growth & development, Culture Media, Polymyxin B/*pharmacology, Polymyxins/*pharmacology, Protoplasts/drug effects, Pseudomonas aeruginosa/*drug effects/growth & development, Spheroplasts/drug effects, Time Factors | Cultivation |
Enzymology | 2155216 | Inactivation of cytochrome cd1 by hydrazines. | Yap-Bondoc F, Timkovich R | J Biol Chem | S0021-9258(19)39554-7 | 1990 | Chromatography, Gel, Chromatography, High Pressure Liquid, Cytochromes, Electron Transport Complex IV/*antagonists & inhibitors/metabolism, Hydrazines/*pharmacology, Hydroquinones/pharmacology, Kinetics, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Structure, Monomethylhydrazine/pharmacology, *Nitrite Reductases, Oxygen Consumption, Phenylhydrazines/pharmacology, Pseudomonas/*analysis, Spectrophotometry | Metabolism |
Enzymology | 2165802 | NMR comparison of prokaryotic and eukaryotic cytochromes c. | Chau MH, Cai ML, Timkovich R | Biochemistry | 10.1021/bi00473a012 | 1990 | Amino Acid Sequence, Animals, *Bacterial Proteins, Cytochrome c Group/*analysis, Eukaryotic Cells/enzymology, Horses, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Pseudomonas aeruginosa/*enzymology, X-Ray Diffraction | Genetics |
Enzymology | 2176826 | 15N NMR spectroscopy of Pseudomonas cytochrome c-551. | Timkovich R | Biochemistry | 10.1021/bi00485a029 | 1990 | *Bacterial Proteins, Cytochrome c Group/*chemistry, Hydrogen, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Nitrogen Isotopes, Protein Conformation, Pseudomonas aeruginosa/*enzymology, Temperature | |
Enzymology | 2513250 | Outer membrane proteins of gentamicin induced small colony variants of Pseudomonas aeruginosa. | Langford PR, Anwar H, Gonda I, Brown MR | FEMS Microbiol Lett | 10.1016/0378-1097(89)90165-1 | 1989 | Bacterial Outer Membrane Proteins/*analysis, Genetic Variation, Gentamicins/*pharmacokinetics, Microbial Sensitivity Tests, Pseudomonas aeruginosa/*drug effects/genetics | Pathogenicity |
Enzymology | 2992381 | 1H NMR spectroscopy of cytochrome cd1 derivatives. | Timkovich R, Cork MS, Taylor PV | Arch Biochem Biophys | 10.1016/0003-9861(85)90077-3 | 1985 | Azides, Cyanides, Cytochromes, *Electron Transport Complex IV, Fluorides, Magnetic Resonance Spectroscopy, *Nitrite Reductases, Pseudomonas aeruginosa/enzymology | |
Pathogenicity | 4218945 | Effect of ethylenediaminetetraacetic acid and related chelating agents on whole cells of gram-negative bacteria. | Haque H, Russell AD | Antimicrob Agents Chemother | 10.1128/AAC.5.5.447 | 1974 | Bacteria/cytology/*drug effects, Chelating Agents/*pharmacology, Edetic Acid/*pharmacology | |
Enzymology | 6091654 | Patterns of product inhibition for bacterial nitrite reductase. | Dhesi R, Timkovich R | Biochem Biophys Res Commun | 10.1016/s0006-291x(84)80228-4 | 1984 | Aerobiosis, Anaerobiosis, Cytochromes, Electron Transport Complex IV/isolation & purification/*metabolism, Feedback, Kinetics, NADH, NADPH Oxidoreductases/*antagonists & inhibitors, Nitrite Reductases/*antagonists & inhibitors, Oxygen Consumption, Paracoccus denitrificans/*enzymology, Pseudomonas/*enzymology, Spectrophotometry | Phylogeny |
Enzymology | 6420411 | Proposed structure for the noncovalently associated heme prosthetic group of dissimilatory nitrite reductases. Identification of substituents. | Timkovich R, Cork MS, Taylor PV | J Biol Chem | S0021-9258(17)43448-X | 1984 | Heme/*analysis, Magnetic Resonance Spectroscopy, Molecular Conformation, NADH, NADPH Oxidoreductases/*metabolism, Nitrite Reductases/*metabolism, Paracoccus denitrificans/*enzymology, Pseudomonas aeruginosa/*enzymology, Spectrophotometry, Structure-Activity Relationship | Metabolism |
Pathogenicity | 11714006 | Antimicrobial activity of Mallotus oppositifolium extractives. | Ogundipe OO, Moody JO, Fakeye TO, Ladipo OB | Afr J Med Med Sci | 2000 | Biological Assay, Chemical Fractionation/methods, Chromatography, Thin Layer, Drug Evaluation, Preclinical, Fungi/*drug effects, Gram-Negative Bacteria/*drug effects, Gram-Positive Bacteria/*drug effects, Humans, *Medicine, African Traditional, Microbial Sensitivity Tests, Nigeria, Plant Extracts/chemistry, Plants, Medicinal/*chemistry/*physiology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1322 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3227) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3227 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
37963 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17074 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68369 | Automatically annotated from API 20NE | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
81973 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45626.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116085 | Curators of the CIP | Collection of Institut Pasteur (CIP 105094) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105094 |