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Strain identifier

BacDive ID: 12764

Type strain: No

Species: Pseudomonas aeruginosa

Strain history: <- NCTC <- H.L. Schutze

NCBI tax ID(s): 287 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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@ref: 1322

BacDive-ID: 12764

DSM-Number: 3227

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, antibiotic resistance, human pathogen

description: Pseudomonas aeruginosa DSM 3227 is a mesophilic human pathogen that has multiple antibiotic resistances and was isolated from urine.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history: <- NCTC <- H.L. Schutze

doi: 10.13145/bacdive12764.20221219.7.1

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 1322

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

type strain: no


cell morphology

@refmotilityconfidencegram stain

Culture and growth conditions

culture medium

1322NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
37963MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp


Physiology and metabolism


  • @ref: 1322
  • medium: Mueller-Hinton Agar
  • incubation temperature: 37
  • incubation time: 1
  • oxygen condition: aerob
  • Penicillin G: 0
  • oxacillin: 0
  • ampicillin: 0
  • ticarcillin: 24-26
  • mezlocillin: 14
  • cefalotin: 0
  • cefazolin: 0
  • cefotaxime: 18
  • aztreonam: 24
  • imipenem: 28
  • tetracycline: 12
  • chloramphenicol: 10-12
  • gentamycin: 20
  • amikacin: 24
  • vancomycin: 0
  • erythromycin: 10
  • lincomycin: 0
  • ofloxacin: 20-22
  • norfloxacin: 30
  • colistin: 14
  • pipemidic acid: 16
  • nitrofurantoin: 0
  • bacitracin: 0
  • polymyxin b: 16
  • kanamycin: 12
  • neomycin: 16-18
  • doxycycline: 10
  • ceftriaxone: 22
  • clindamycin: 0
  • fosfomycin: 34
  • moxifloxacin: 20
  • linezolid: 0
  • nystatin: 0
  • quinupristin/dalfopristin: 0
  • teicoplanin: 0
  • piperacillin/tazobactam: 24

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 95.918


  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 91.13

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836927897tryptophan-energy source

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -


68369cytochrome oxidase+
68369arginine dihydrolase+



Isolation, sampling and environmental information


  • @ref: 1322
  • sample type: urine

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids
  • Cat3: #Urine


  • @ref: 69479
  • File name: preview.99_42.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_36;97_37;98_40;99_42&stattab=map
  • Last taxonomy: Pseudomonas aeruginosa
  • 16S sequence: AB680129
  • Sequence Identity:
  • Total samples: 22757
  • soil counts: 1669
  • aquatic counts: 5046
  • animal counts: 14748
  • plant counts: 1294

Safety information

risk assessment

  • @ref: 1322
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Pseudomonas aeruginosa gene for 16S rRNA, partial sequence, strain: NBRC 3755
  • accession: AB680129
  • length: 1461
  • database: ena
  • NCBI tax ID: 287

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas aeruginosa NCTC6750GCA_900461635contigncbi287
66792Pseudomonas aeruginosa strain NCTC6750287.6523wgspatric287
66792Escherichia coli MGH207GCA_014903235scaffoldpatric562

External links

@ref: 1322

culture collection no.: DSM 3227, ATCC 19429, IFO 3755, NBRC 3755, NCIB 6750, NCTC 6750

straininfo link



Pathogenicity34692Additivity of action between polysorbate 80 and polymyxin B towards spheroplasts of Pseudomonas aeruginosa NCTC 6750.Brown MR, Geaton EM, Gilbert PJ Pharm Pharmacol10.1111/j.2042-7158.1979.tb13463.x1979Polyethylene Glycols/*pharmacology, Polymyxin B/*pharmacology, Polymyxins/*pharmacology, Polysorbates/*pharmacology, Pseudomonas aeruginosa/*drug effects/growth & development/ultrastructure, Spheroplasts/*drug effects, Time Factors
Pathogenicity218501Influence of suspending media upon the susceptibility of Pseudomonas aeruginosa NCTC 6750 and its spheroplasts to polymyxin B.Klemperer RM, Gilbert P, Meier AM, Cozens RM, Brown MRAntimicrob Agents Chemother10.1128/AAC.15.2.1471979Bacillus megaterium/drug effects/growth & development, Culture Media, Polymyxin B/*pharmacology, Polymyxins/*pharmacology, Protoplasts/drug effects, Pseudomonas aeruginosa/*drug effects/growth & development, Spheroplasts/drug effects, Time FactorsCultivation
Enzymology2155216Inactivation of cytochrome cd1 by hydrazines.Yap-Bondoc F, Timkovich RJ Biol ChemS0021-9258(19)39554-71990Chromatography, Gel, Chromatography, High Pressure Liquid, Cytochromes, Electron Transport Complex IV/*antagonists & inhibitors/metabolism, Hydrazines/*pharmacology, Hydroquinones/pharmacology, Kinetics, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Structure, Monomethylhydrazine/pharmacology, *Nitrite Reductases, Oxygen Consumption, Phenylhydrazines/pharmacology, Pseudomonas/*analysis, SpectrophotometryMetabolism
Enzymology2165802NMR comparison of prokaryotic and eukaryotic cytochromes c.Chau MH, Cai ML, Timkovich RBiochemistry10.1021/bi00473a0121990Amino Acid Sequence, Animals, *Bacterial Proteins, Cytochrome c Group/*analysis, Eukaryotic Cells/enzymology, Horses, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Pseudomonas aeruginosa/*enzymology, X-Ray DiffractionGenetics
Enzymology217682615N NMR spectroscopy of Pseudomonas cytochrome c-551.Timkovich RBiochemistry10.1021/bi00485a0291990*Bacterial Proteins, Cytochrome c Group/*chemistry, Hydrogen, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Nitrogen Isotopes, Protein Conformation, Pseudomonas aeruginosa/*enzymology, Temperature
Enzymology2513250Outer membrane proteins of gentamicin induced small colony variants of Pseudomonas aeruginosa.Langford PR, Anwar H, Gonda I, Brown MRFEMS Microbiol Lett10.1016/0378-1097(89)90165-11989Bacterial Outer Membrane Proteins/*analysis, Genetic Variation, Gentamicins/*pharmacokinetics, Microbial Sensitivity Tests, Pseudomonas aeruginosa/*drug effects/geneticsPathogenicity
Enzymology29923811H NMR spectroscopy of cytochrome cd1 derivatives.Timkovich R, Cork MS, Taylor PVArch Biochem Biophys10.1016/0003-9861(85)90077-31985Azides, Cyanides, Cytochromes, *Electron Transport Complex IV, Fluorides, Magnetic Resonance Spectroscopy, *Nitrite Reductases, Pseudomonas aeruginosa/enzymology
Pathogenicity4218945Effect of ethylenediaminetetraacetic acid and related chelating agents on whole cells of gram-negative bacteria.Haque H, Russell ADAntimicrob Agents Chemother10.1128/AAC.5.5.4471974Bacteria/cytology/*drug effects, Chelating Agents/*pharmacology, Edetic Acid/*pharmacology
Enzymology6091654Patterns of product inhibition for bacterial nitrite reductase.Dhesi R, Timkovich RBiochem Biophys Res Commun10.1016/s0006-291x(84)80228-41984Aerobiosis, Anaerobiosis, Cytochromes, Electron Transport Complex IV/isolation & purification/*metabolism, Feedback, Kinetics, NADH, NADPH Oxidoreductases/*antagonists & inhibitors, Nitrite Reductases/*antagonists & inhibitors, Oxygen Consumption, Paracoccus denitrificans/*enzymology, Pseudomonas/*enzymology, SpectrophotometryPhylogeny
Enzymology6420411Proposed structure for the noncovalently associated heme prosthetic group of dissimilatory nitrite reductases. Identification of substituents.Timkovich R, Cork MS, Taylor PVJ Biol ChemS0021-9258(17)43448-X1984Heme/*analysis, Magnetic Resonance Spectroscopy, Molecular Conformation, NADH, NADPH Oxidoreductases/*metabolism, Nitrite Reductases/*metabolism, Paracoccus denitrificans/*enzymology, Pseudomonas aeruginosa/*enzymology, Spectrophotometry, Structure-Activity RelationshipMetabolism
Pathogenicity11714006Antimicrobial activity of Mallotus oppositifolium extractives.Ogundipe OO, Moody JO, Fakeye TO, Ladipo OBAfr J Med Med Sci2000Biological Assay, Chemical Fractionation/methods, Chromatography, Thin Layer, Drug Evaluation, Preclinical, Fungi/*drug effects, Gram-Negative Bacteria/*drug effects, Gram-Positive Bacteria/*drug effects, Humans, *Medicine, African Traditional, Microbial Sensitivity Tests, Nigeria, Plant Extracts/chemistry, Plants, Medicinal/*chemistry/*physiology


1322Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3227)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3227
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37963Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17074
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)