Name: Geobacillus stearothermophilus C953
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1448

Type strain:

Species: Geobacillus stearothermophilus

Strain Designation: C953

Culture col. no.: CCUG 34144, HNCMB 101025, LMG 11163, DSM 1550, JCM 12216, CIP 67.5, IAM 12043, JCM 14450, NBIMCC 2330, NBRC 100862, NCDO 1780, NCIMB 11780

Strain history: CIP <- 1967, J.O. Clair, CNRS, Jouy en Josas, France: strain C 953, Bacillus stearothermophilus var. calidolactis

NCBI tax ID(s): 1422 (species)

SI-ID 12230

ATCC 10149, DSM 6790, LMG 11163, CCM 5965, CECT 47, DSM 1550, IAM 12043, LMG 17903, NCFB 1780, NCIMB 11780, JCM 11297, CCUG 620, CCUG 34144, NCDO 1780, NBIMCC 2330, CIP 105453, NCIMB 13693, VKM B-2101, CIP 67.5, LMD 74.1, CCRC 10610, CCRC 12309, CECT 4517, JCM 12216, NBRC 100862, BCRC 10610, BCRC 12309, IAM 11004, CBS 706.83, LMG 17962, JCM 14450, CNCTC 6228

Special Collections

Other strains from Geobacillus stearothermophilus

Section

Geobacillus stearothermophilus C953 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from evaporated milk.

Gram-positive
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Geobacillus*
	Species	**Geobacillus stearothermophilus**
	Full Scientific Name (LPSN)	Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

Synonym

Bacillus stearothermophilus

Information on morphological and physiological properties Morphology

[Ref.: #123296]	Gram stain	positive

[Ref.: #123296]	Cell shape	rod-shaped

[Ref.: #123296]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #817]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #817]

Culture medium growth

[Ref.: #817]

Culture medium link

Medium recipe at MediaDive

[Ref.: #817]

Culture medium composition

expand / minimize

[Ref.: #35015]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #35015]

Culture medium growth

[Ref.: #35015]

Culture medium composition

expand / minimize

[Ref.: #123296]

Culture medium

CIP Medium 72

[Ref.: #123296]

Culture medium growth

[Ref.: #123296]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123296]

Culture medium

CIP Medium 29

[Ref.: #123296]

Culture medium growth

[Ref.: #123296]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123296]

Culture medium

CIP Medium 3

[Ref.: #123296]

Culture medium growth

[Ref.: #123296]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #817]		growth	55 °C
[Ref.: #35015]		growth	55 °C
[Ref.: #51955]		growth	55 °C
[Ref.: #67770]		growth	55 °C
[Ref.: #123296]		growth	45-55 °C
[Ref.: #123296]	no	growth	10 °C
[Ref.: #123296]	no	growth	22 °C
[Ref.: #123296]	no	growth	30 °C
[Ref.: #123296]	no	growth	37 °C

	pH	Kind of pH		pH
[Ref.: #123296]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #51955]	Oxygen tolerance	aerobe
[Ref.: #123296]	Oxygen tolerance	facultative anaerobe

[Ref.: #123296]

Ability of spore formation

yes

	Salt	Tested relation		Salt conc.
[Ref.: #123296]	NaCl		growth	0-2	%
[Ref.: #123296]	NaCl	no	growth	4	%
[Ref.: #123296]	NaCl	no	growth	6	%
[Ref.: #123296]	NaCl	no	growth	8	%
[Ref.: #123296]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

Assay of Benzylpenicillin

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123296]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123296]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #123296]	hippurate ChEBI	+	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123296]	nitrate ChEBI	-	reduction
[Ref.: #123296]	nitrate ChEBI	+	respiration
[Ref.: #123296]	nitrite ChEBI	+	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123296]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123296]		acetoin ChEBI	-
[Ref.: #123296]		glucose ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #123296]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123296]	amylase	+
[Ref.: #123296]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123296]	caseinase	+	3.4.21.50
[Ref.: #123296]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #123296]	DNase	+
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123296]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #123296]	gelatinase	+/-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123296]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #123296]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123296]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123296]	tween esterase	+
[Ref.: #123296]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #123296]	12070	not determinedn.d.	+/-	-	-	-	+/-	-	-	-	-	+	+	+	+	+/-	-	-	-	-	-	-	+/-	-	-	-	+/-	+	+/-	+	+	+	+	+/-	-	+	+	+	+	-	-	+	-	+	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123296]	13127	-	-	-	+	-	-	-	-	-	+	-	-	+	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #817]	Sample type/isolated from	evaporated milk

[Ref.: #51955]	Sample type/isolated from	Milk,evaporated / coffee cream (NIZO)
[Ref.: #51955]	Geographic location (country and/or sea, region)	probably in Ede or Hoorn
[Ref.: #51955]	Country	Netherlands
[Ref.: #51955]	Country ISO 3 Code	NLD
[Ref.: #51955]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Evaporated milk

[Ref.: #123296]	Sample type/isolated from	Food, Milk
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Dairy product

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB598739 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	603
Soil Samples	387
Animal Samples	2094
Plant Samples	132
Total Samples	3216

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #817]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123296]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Geobacillus stearothermophilus gene for 16S rRNA, partial sequence, strain: JCM 12216	AB598739	1481	GenBank ENA	1422 tax ID
[Ref.: #124043]	Geobacillus stearothermophilus gene for 16S rRNA, partial sequence, strain: NBRC 100862.	AB681268	1484	GenBank ENA	1422 tax ID	*

Availability in culture collections External links

[Ref.: #817]

Culture collection no.

CCUG 34144, HNCMB 101025, LMG 11163, DSM 1550, JCM 12216, CIP 67.5, IAM 12043, JCM 14450, NBIMCC 2330, NBRC 100862, NCDO 1780, NCIMB 11780

[Ref.: #71098]

SI-ID 12230

References

#817	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1550

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#35015 ; Curators of the CIP;
#51955 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 34144
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71098

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12230.1 )

#123296 Collection of Institut Pasteur ; Curators of the CIP; CIP 67.5

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

Change proposal

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