Geobacillus stearothermophilus (CCUG 34144, HNCMB 101025, LMG 11163)

Name: Geobacillus stearothermophilus (CCUG 34144, HNCMB 101025, LMG 11163)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1448

Type strain

Strain Designation C953

Culture col. no. CCUG 34144 HNCMB 101025 LMG 11163 DSM 1550 C953 8 more

NCBI tax ID(s) 1422

Special Collections DSMZ CCUG JCM CIP

Links

Geobacillus stearothermophilus C953 is an aerobe, spore-forming, thermophilic prokaryote that was isolated from evaporated milk.

spore-forming Gram-positive rod-shaped aerobe thermophilic 16S sequence

Name and taxonomic classification

SI-ID 12230

ATCC 10149,DSM 6790,LMG 11163,CCM 5965,CECT 47,DSM 1550,IAM 12043,LMG 17903,NCFB 1780,NCIMB 11780,JCM 11297,CCUG 620,CCUG 34144,NCDO 1780,NBIMCC 2330,CIP 105453,NCIMB 13693,VKM B-2101,CIP 67.5,LMD 74.1,CCRC 10610,CCRC 12309,CECT 4517,JCM 12216,NBRC 100862,BCRC 10610,BCRC 12309,IAM 11004,CBS 706.83,LMG 17962,JCM 14450,CNCTC 6228

@ref 20215

Last LPSN update 2025-12-16 09:47:06

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus stearothermophilus

Full scientific name Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

Synonyms (1)

Bacillus stearothermophilus

BacDive ID	Other strains from **Geobacillus stearothermophilus** (22)	Type strain
1419	G. stearothermophilus R-35646, 26, DSM 22, ATCC 12980, CCM 2062, ... (type strain)
1410	G. stearothermophilus DSM 297, ATCC 12976, CCM 2183, NCIB 8919
1411	G. stearothermophilus L 32-65, DSM 456
1412	G. stearothermophilus L 33-65, R-32640, DSM 457
1413	G. stearothermophilus R-32765, S 65-67, DSM 458
1414	G. stearothermophilus DSM 494, NCTC 10003, CCUG 11734
1415	G. stearothermophilus R-32607, alpha 1, DSM 2357, NCIMB 10279
1416	G. stearothermophilus alpha 7, DSM 2358, NCIB 10280
1417	G. stearothermophilus NRS-19, 1503, DSM 2027, ATCC 7954, NCIB 8924, ...
1418	G. stearothermophilus 186, DSM 2313, NCIB 10278
1420	G. stearothermophilus N.R. Smith T17, 1518, DSM 5934, ATCC 7953, ...
1421	G. stearothermophilus T15, DSM 6790, ATCC 10149, WDCM 00069, CCUG ...
1444	G. stearothermophilus 10, DSM 13240, CIP 106956
100611	G. stearothermophilus STH00420(ZIMET), CMI 98373, ACC 1829, HKI ...
134733	G. stearothermophilus CIP 57.16, NBIMCC 2329
142398	G. stearothermophilus CCUG 12317
149136	G. stearothermophilus CCUG 36912
151704	G. stearothermophilus CCUG 44677
154550	G. stearothermophilus CCUG 54374
156003	G. stearothermophilus CCUG 59982
161282	G. stearothermophilus JCM 12894
163708	G. stearothermophilus JCM 30104

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123296	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
817	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
35015	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
123296	CIP Medium 72	Medium recipe at CIP
123296	CIP Medium 29	Medium recipe at CIP
123296	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
817	positive	growth	55	thermophilic
35015	positive	growth	55	thermophilic
51955	positive	growth	55	thermophilic
67770	positive	growth	55	thermophilic
123296	positive	growth	45-55	thermophilic
123296	negative	growth	10
123296	negative	growth	22
123296	negative	growth	30
123296	negative	growth	37

Culture pH

@ref	Ability	Type	PH
123296	positive	growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
51955	aerobe
123296	facultative anaerobe

Spore formation

123296

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123296	NaCl	positive	growth	0-2 %
123296	NaCl		growth	4 %
123296	NaCl		growth	6 %
123296	NaCl		growth	8 %
123296	NaCl		growth	10 %

Observation

67770

ObservationAssay of Benzylpenicillin

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
123296	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
123296	4853 ChEBI	esculin	+	hydrolysis
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
123296	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
123296	17632 ChEBI	nitrate	-	reduction
123296	17632 ChEBI	nitrate	+	respiration
123296	16301 ChEBI	nitrite	+	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123296	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123296	15688 ChEBI	acetoin	-
123296	17234 ChEBI	glucose		+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
123296	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123296	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
123296	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123296	caseinase	+	3.4.21.50
123296	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123296	DNase	+
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
123296	gamma-glutamyltransferase	-	2.3.2.2
123296	gelatinase	+/-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123296	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
123296	ornithine decarboxylase	-	4.1.1.17
123296	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
123296	tween esterase	+
123296	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123296	-	-	-	+	-	-	-	-	-	+	-	-	+	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123296	not determinedn.d.	+/-	-	-	-	+/-	-	-	-	-	+	+	+	+	+/-	-	-	-	-	-	-	+/-	-	-	-	+/-	+	+/-	+	+	+	+	+/-	-	+	+	+	+	-	-	+	-	+	-	-	-	-	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Dairy product

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
817	evaporated milk
51955	Milk,evaporated / coffee cream (NIZO)	probably in Ede or Hoorn	Netherlands	NLD	Europe
67770	Evaporated milk
123296	Food, Milk

Taxonmaps

69479

Global distribution of 16S sequence AB598739 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	603
Soil Samples	387
Animal Samples	2094
Plant Samples	132
Total Samples	3216

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
817	1	Risk group (German classification) According to TRBA 466
123296	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Geobacillus stearothermophilus gene for 16S rRNA, partial sequence, strain: JCM 12216	AB598739	1481		1422
124043	Geobacillus stearothermophilus gene for 16S rRNA, partial sequence, strain: NBRC 100862.	AB681268	1484		1422

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Cyclic lipodepsipeptides produced by Pseudomonas spp. naturally present in raw milk induce inhibitory effects on microbiological inhibitor assays for antibiotic residue screening.	Reybroeck W, De Vleeschouwer M, Marchand S, Sinnaeve D, Heylen K, De Block J, Madder A, Martins JC, Heyndrickx M.	PLoS One	10.1371/journal.pone.0098266	2014
	Effective ultraviolet C light disinfection of respirators demonstrated in challenges with Geobacillus stearothermophilus spores and SARS-CoV-2 virus.	van der Vossen JMBM, Fawzy A, Ouwens AMT, van Doornmalen JPCM, de Samber M, Driessens R, Heerikhuisen M, Montijn RC.	J Hosp Infect	10.1016/j.jhin.2022.01.021	2022
	Direct fermentation of potato starch and potato residues to lactic acid by Geobacillus stearothermophilus under non-sterile conditions.	Smerilli M, Neureiter M, Wurz S, Haas C, Fruhauf S, Fuchs W.	J Chem Technol Biotechnol	10.1002/jctb.4627	2015
	Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells.	Blecha A, Zarschler K, Sjollema KA, Veenhuis M, Rodel G.	Microb Cell Fact	10.1186/1475-2859-4-28	2005

References

#817	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1550
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#35015	; Curators of the CIP;
#51955	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 34144
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123296	Collection of Institut Pasteur ; Curators of the CIP; CIP 67.5
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Geobacillus stearothermophilus (CCUG 34144, HNCMB 101025, LMG 11163)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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