Geobacillus stearothermophilus (DSM 2027, ATCC 7954, NCIB 8924)

Name: Geobacillus stearothermophilus (DSM 2027, ATCC 7954, NCIB 8924)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1417

Type strain

Strain Designation NRS-19, 1503

Culture col. no. DSM 2027 ATCC 7954 NCIB 8924 CIP 52.80 ATCC 29609 4 more

NCBI tax ID(s) 1422

Special Collections DSMZ CIP

History <- NCIB <- ATCC <- C.P. Hegarty <- NCA 1503 CIP <- 1952? R.E. Gordon, New Jersey Agri. Exper. Stat., New Brunswick, USA: strain NRS-19 <- 1944, Curran <- NCA: strain 1503

Links

Geobacillus stearothermophilus NRS-19 is a facultative anaerobe, Gram-positive, motile bacterium that was isolated from canned peas.

Gram-positive motile rod-shaped facultative anaerobe Bacteria

Name and taxonomic classification

SI-ID 39613

ATCC 29609,ATCC 7954,DSM 2027,IAM 11003,NCIB 8924,NCIMB 8924,CIP 52.80,BCRC 11732,CCRC 11732,VTT E-81129

@ref 20215

Last LPSN update 2026-02-09 11:18:06

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus stearothermophilus

Full scientific name Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

Synonyms (1)

Bacillus stearothermophilus

BacDive ID	Other strains from **Geobacillus stearothermophilus** (22)	Type strain
1419	G. stearothermophilus R-35646, 26, DSM 22, ATCC 12980, CCM 2062, ... (type strain)
1410	G. stearothermophilus DSM 297, ATCC 12976, CCM 2183, NCIB 8919
1411	G. stearothermophilus L 32-65, DSM 456
1412	G. stearothermophilus L 33-65, R-32640, DSM 457
1413	G. stearothermophilus R-32765, S 65-67, DSM 458
1414	G. stearothermophilus DSM 494, NCTC 10003, CCUG 11734
1415	G. stearothermophilus R-32607, alpha 1, DSM 2357, NCIMB 10279
1416	G. stearothermophilus alpha 7, DSM 2358, NCIB 10280
1418	G. stearothermophilus 186, DSM 2313, NCIB 10278
1420	G. stearothermophilus N.R. Smith T17, 1518, DSM 5934, ATCC 7953, ...
1421	G. stearothermophilus T15, DSM 6790, ATCC 10149, WDCM 00069, CCUG ...
1444	G. stearothermophilus 10, DSM 13240, CIP 106956
1448	G. stearothermophilus C953, CCUG 34144, HNCMB 101025, LMG 11163, ...
100611	G. stearothermophilus STH00420(ZIMET), CMI 98373, ACC 1829, HKI ...
134733	G. stearothermophilus CIP 57.16, NBIMCC 2329
142398	G. stearothermophilus CCUG 12317
149136	G. stearothermophilus CCUG 36912
151704	G. stearothermophilus CCUG 44677
154550	G. stearothermophilus CCUG 54374
156003	G. stearothermophilus CCUG 59982
161282	G. stearothermophilus JCM 12894
163708	G. stearothermophilus JCM 30104

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123297	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
960	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
38723	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
123297	CIP Medium 72	Medium recipe at CIP
123297	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
960	positive	growth	55
38723	positive	growth	50

Physiology and metabolism

Oxygen tolerance

123297

Oxygen tolerancefacultative anaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
123297	17632 ChEBI	nitrate	+	reduction
123297	16301 ChEBI	nitrite	+	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123297	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123297	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123297	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
123297	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123297	-	-	+	+	-	+	-	-	-	+	+	+	+	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123297	not determinedn.d.	+	-	-	-	+/-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	+	+	+	-	+	+	+	+	-	-	+	-	+/-	-	-	-	-	-	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Bottled, canned, packed food
#Engineered	#Food production	#Food
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type
960	canned peas
123297	Canned peas

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
960	1	Risk group (German classification) According to TRBA 466
123297	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system.	Cai X, Lin L, Shen Y, Wei W, Wei DZ.	BMC Biotechnol	10.1186/s12896-020-00622-1	2020
	Screening Methods for Antimicrobial Residues in the Dairy Chain-The Past and the Present.	Navratilova P, Vorlova L, Dluhosova S, Bartakova K, Hanus O, Samkova E.	Antibiotics (Basel)	10.3390/antibiotics13111098	2024
	Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production.	Irla M, Heggeset TM, Naerdal I, Paul L, Haugen T, Le SB, Brautaset T, Wendisch VF.	Front Microbiol	10.3389/fmicb.2016.01481	2016
Metabolism	Deciphering the Regulation of the Mannitol Operon Paves the Way for Efficient Production of Mannitol in Lactococcus lactis.	Xiao H, Bang-Berthelsen CH, Jensen PR, Solem C.	Appl Environ Microbiol	10.1128/aem.00779-21	2021
Metabolism	Nonclassical protein secretion by Bacillus subtilis in the stationary phase is not due to cell lysis.	Yang CK, Ewis HE, Zhang X, Lu CD, Hu HJ, Pan Y, Abdelal AT, Tai PC.	J Bacteriol	10.1128/jb.05897-11	2011
Enzymology	Cloning and characterization of acetohydroxyacid synthase from Bacillus stearothermophilus.	Porat I, Vinogradov M, Vyazmensky M, Lu CD, Chipman DM, Abdelal AT, Barak Z.	J Bacteriol	10.1128/jb.186.2.570-574.2004	2004
Enzymology	Characterization of a novel thermostable carboxylesterase from Geobacillus kaustophilus HTA426 shows the existence of a new carboxylesterase family.	Montoro-Garcia S, Martinez-Martinez I, Navarro-Fernandez J, Takami H, Garcia-Carmona F, Sanchez-Ferrer A.	J Bacteriol	10.1128/jb.01060-08	2009
	Bacillus stearothermophilus. II. Certain factors affecting amylase production on some undefined media.	HARTMAN PA, TETRAULT PA.	Appl Microbiol	10.1128/am.3.1.11-14.1955	1955
Metabolism	Cloning, expression, and isolation of the mannitol transport protein from the thermophilic bacterium Bacillus stearothermophilus.	Henstra SA, Tolner B, ten Hoeve Duurkens RH, Konings WN, Robillard GT.	J Bacteriol	10.1128/jb.178.19.5586-5591.1996	1996
	Regulation of groE expression in Bacillus subtilis: the involvement of the sigma A-like promoter and the roles of the inverted repeat sequence (CIRCE).	Yuan G, Wong SL.	J Bacteriol	10.1128/jb.177.19.5427-5433.1995	1995
	Bacillus stearothermophilus. I. Thermal and pH stability of the amylase.	HARTMAN PA, WELLERSON R, TETRAULT PA.	Appl Microbiol	10.1128/am.3.1.7-10.1955	1955
Cultivation	Development of defined and minimal media for the growth of Bacillus stearothermophilus.	Rowe JJ, Goldberg ID, Amelunxen RE.	J Bacteriol	10.1128/jb.124.1.279-284.1975	1975
Metabolism	Comparative study of energy-transducing properties of cytoplasmic membranes from mesophilic and thermophilic Bacillus species.	De Vrij W, Bulthuis RA, Konings WN.	J Bacteriol	10.1128/jb.170.5.2359-2366.1988	1988
Metabolism	Mechanism of L-glutamate transport in membrane vesicles from Bacillus stearothermophilus.	de Vrij W, Bulthuis RA, van Iwaarden PR, Konings WN.	J Bacteriol	10.1128/jb.171.2.1118-1125.1989	1989
Metabolism	Sodium ion-dependent amino acid transport in membrane vesicles of Bacillus stearothermophilus.	Heyne RI, de Vrij W, Crielaard W, Konings WN.	J Bacteriol	10.1128/jb.173.2.791-800.1991	1991
Enzymology	Thermostable polynucleotide phosphorylases from Bacillus stearothermophilus and Thermus aquaticus.	Wood JN, Hutchinson DW.	Nucleic Acids Res	10.1093/nar/3.1.219	1976
	The nucleoside sequence of tyrosine tRNA from Bacillus stearothermophilus.	Brown RS, Rubin JR, Rhodes D, Guilley H, Simoncsits A, Brownlee GG.	Nucleic Acids Res	10.1093/nar/5.1.23	1978
	Isolation of a Bacillus stearothermophilus mutant exhibiting increased thermostability in its restriction endonuclease.	Hendrix JD, Welker NE.	J Bacteriol	10.1128/jb.162.2.682-692.1985	1985
Phylogeny	Nature of polymorphisms in 16S-23S rRNA gene intergenic transcribed spacer fingerprinting of Bacillus and related genera.	Daffonchio D, Cherif A, Brusetti L, Rizzi A, Mora D, Boudabous A, Borin S.	Appl Environ Microbiol	10.1128/aem.69.9.5128-5137.2003	2003
Enzymology	Clamp-loader-helicase interaction in Bacillus. Leucine 381 is critical for pentamerization and helicase binding of the Bacillus tau protein.	Haroniti A, Till R, Smith MC, Soultanas P.	Biochemistry	10.1021/bi034955g	2003
Enzymology	Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus.	Ewis HE, Abdelal AT, Lu CD	Gene	10.1016/j.gene.2003.12.029	2004
Enzymology	Overexpression, purification, and characterization of the recombinant leucine aminopeptidase II of Bacillus stearothermophilus.	Kuo LY, Hwang GY, Lai YJ, Yang SL, Lin LL	Curr Microbiol	10.1007/s00284-002-3950-z	2003
Enzymology	Cloning of genes of the aminopeptidase T family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924: apparent similarity to the leucyl aminopeptidase family.	Motoshima H, Minagawa E, Tsukasaki F, Kaminogawa S	Biosci Biotechnol Biochem	10.1271/bbb.61.1710	1997

References

#960	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2027
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#38723	; Curators of the CIP;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#123297	Collection of Institut Pasteur ; Curators of the CIP; CIP 52.80
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Geobacillus stearothermophilus (DSM 2027, ATCC 7954, NCIB 8924)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

BacDive

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