Geobacillus stearothermophilus (DSM 13240, CIP 106956, 10)

Name: Geobacillus stearothermophilus (DSM 13240, CIP 106956, 10)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1444

Type strain

Strain Designation 10

Culture col. no. DSM 13240 CIP 106956 10

NCBI tax ID(s) 1422

Special Collections DSMZ CIP

Links

Geobacillus stearothermophilus 10 is an obligate aerobe, spore-forming, thermophilic prokaryote that was isolated from hot springs.

spore-forming Gram-positive rod-shaped obligate aerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 307563

NBIMCC 2282,CIP 106956,DSM 13240,BCRC 17458,CCRC 17458

@ref 20215

Last LPSN update 2025-12-16 09:47:06

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus stearothermophilus

Full scientific name Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

Synonyms (1)

Bacillus stearothermophilus

BacDive ID	Other strains from **Geobacillus stearothermophilus** (22)	Type strain
1419	G. stearothermophilus R-35646, 26, DSM 22, ATCC 12980, CCM 2062, ... (type strain)
1410	G. stearothermophilus DSM 297, ATCC 12976, CCM 2183, NCIB 8919
1411	G. stearothermophilus L 32-65, DSM 456
1412	G. stearothermophilus L 33-65, R-32640, DSM 457
1413	G. stearothermophilus R-32765, S 65-67, DSM 458
1414	G. stearothermophilus DSM 494, NCTC 10003, CCUG 11734
1415	G. stearothermophilus R-32607, alpha 1, DSM 2357, NCIMB 10279
1416	G. stearothermophilus alpha 7, DSM 2358, NCIB 10280
1417	G. stearothermophilus NRS-19, 1503, DSM 2027, ATCC 7954, NCIB 8924, ...
1418	G. stearothermophilus 186, DSM 2313, NCIB 10278
1420	G. stearothermophilus N.R. Smith T17, 1518, DSM 5934, ATCC 7953, ...
1421	G. stearothermophilus T15, DSM 6790, ATCC 10149, WDCM 00069, CCUG ...
1448	G. stearothermophilus C953, CCUG 34144, HNCMB 101025, LMG 11163, ...
100611	G. stearothermophilus STH00420(ZIMET), CMI 98373, ACC 1829, HKI ...
134733	G. stearothermophilus CIP 57.16, NBIMCC 2329
142398	G. stearothermophilus CCUG 12317
149136	G. stearothermophilus CCUG 36912
151704	G. stearothermophilus CCUG 44677
154550	G. stearothermophilus CCUG 54374
156003	G. stearothermophilus CCUG 59982
161282	G. stearothermophilus JCM 12894
163708	G. stearothermophilus JCM 30104

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123298	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4979	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Yeast extract 50.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
4979	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34094	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123298	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4979	positive	growth	55	thermophilic
34094	positive	growth	55	thermophilic
123298	positive	growth	45-55	thermophilic
123298	negative	growth	10
123298	negative	growth	22
123298	negative	growth	30
123298	negative	growth	37

Culture pH

@ref	Ability	Type	PH
123298		growth	6

Physiology and metabolism

Oxygen tolerance

123298

Oxygen toleranceobligate aerobe

Spore formation

@ref	Spore formation	Confidence
123298
125438		92.983

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123298	NaCl	positive	growth	0-2 %
123298	NaCl		growth	4 %
123298	NaCl		growth	6 %
123298	NaCl		growth	8 %
123298	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
123298	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
123298	4853 ChEBI	esculin	+	hydrolysis
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
123298	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
123298	17632 ChEBI	nitrate	-	reduction
123298	17632 ChEBI	nitrate	-	respiration
123298	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123298	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123298	15688 ChEBI	acetoin	-
123298	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123298	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123298	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
123298	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123298	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123298	gamma-glutamyltransferase	+	2.3.2.2
123298	gelatinase	+/-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123298	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123298	ornithine decarboxylase	-	4.1.1.17
123298	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
123298	tween esterase	-
123298	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123298	-	-	+	+	-	+	-	-	-	+	+	+	+	+	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123298	not determinedn.d.	-	-	-	-	+/-	+/-	-	-	-	+/-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	+/-	+/-	+/-	-	+/-	+/-	+/-	+/-	+/-	-	+/-	+/-	+/-	+/-	-	-	+/-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4979	hot springs	Montana, Yellow Stone National Park	USA	USA	North America
123298	Environment, Hot springs	Yellowstone, Montana	United States of America	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
123298	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score
66792	ASM127457v1 assembly for Geobacillus stearothermophilus 10	complete	GCA_001274575	2927205132	272567	72

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Geobacillus stearothermophilus strain BGSC 9A21 16S ribosomal RNA gene, complete sequence.	AY297092	1561		1422

GC content

4979

GC-content (mol%)53

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Geobacillus stearothermophilus 10
PATRIC: 272567.8

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	84.90	no
125439	motility	BacteriaNetⓘ	yes	79.30	no
125439	gram_stain	BacteriaNetⓘ	variable	83.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	69.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Geobacillus stearothermophilus 10
PATRIC: 272567.8

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	76.90	no
125438	anaerobic	anaerobicⓘ	no	94.80	no
125438	spore-forming	spore-formingⓘ	yes	92.98	no
125438	aerobic	aerobicⓘ	yes	78.64	yes
125438	thermophilic	thermophileⓘ	yes	67.47	yes
125438	flagellated	motile2+ⓘ	yes	83.59	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Novel transaminases from thermophiles: from discovery to application.	Cardenas-Fernandez M, Sinclair O, Ward JM.	Microb Biotechnol	10.1111/1751-7915.13940	2022
	Nested Formation of Calcium Carbonate Polymorphs in a Bacterial Surface Membrane with a Graded Nanoconfinement: An Evolutionary Strategy to Ensure Bacterial Survival.	Simon P, Pompe W, Gruner D, Sturm E, Ostermann K, Matys S, Vogel M, Rodel G.	ACS Biomater Sci Eng	10.1021/acsbiomaterials.1c01280	2022
	Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius.	Koniuchovaite A, Petkeviciute A, Bernotaite E, Gricajeva A, Gegeckas A, Kalediene L, Kaunietis A.	Front Microbiol	10.3389/fmicb.2023.1207367	2023
Enzymology	Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons.	Sherrill-Mix S, Hwang Y, Roche AM, Glascock A, Weiss SR, Li Y, Haddad L, Deraska P, Monahan C, Kromer A, Graham-Wooten J, Taylor LJ, Abella BS, Ganguly A, Collman RG, Van Duyne GD, Bushman FD.	Genome Biol	10.1186/s13059-021-02387-y	2021
Metabolism	Cadmium ion biosorption by the thermophilic bacteria Geobacillus stearothermophilus and G. thermocatenulatus.	Hetzer A, Daughney CJ, Morgan HW.	Appl Environ Microbiol	10.1128/aem.00295-06	2006
	Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells.	Blecha A, Zarschler K, Sjollema KA, Veenhuis M, Rodel G.	Microb Cell Fact	10.1186/1475-2859-4-28	2005

References

#4979	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13240
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34094	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#123298	Collection of Institut Pasteur ; Curators of the CIP; CIP 106956
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Geobacillus stearothermophilus (DSM 13240, CIP 106956, 10)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001274575

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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