Geobacillus stearothermophilus (DSM 458, R-32765, S 65-67)

Name: Geobacillus stearothermophilus (DSM 458, R-32765, S 65-67)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1413

Type strain

Strain Designation R-32765, S 65-67

Culture col. no. DSM 458 R-32765 S 65-67

NCBI tax ID(s) 1422

Special Collections DSMZ

History <- F. Hollaus, S 65-67

Links

Geobacillus stearothermophilus R-32765 is a bacterium that was isolated from sugar beet juice from extraction installations.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 47188

DSM 458

@ref 20215

Last LPSN update 2026-02-09 11:18:06

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus stearothermophilus

Full scientific name Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

Synonyms (1)

Bacillus stearothermophilus

BacDive ID	Other strains from **Geobacillus stearothermophilus** (22)	Type strain
1419	G. stearothermophilus R-35646, 26, DSM 22, ATCC 12980, CCM 2062, ... (type strain)
1410	G. stearothermophilus DSM 297, ATCC 12976, CCM 2183, NCIB 8919
1411	G. stearothermophilus L 32-65, DSM 456
1412	G. stearothermophilus L 33-65, R-32640, DSM 457
1414	G. stearothermophilus DSM 494, NCTC 10003, CCUG 11734
1415	G. stearothermophilus R-32607, alpha 1, DSM 2357, NCIMB 10279
1416	G. stearothermophilus alpha 7, DSM 2358, NCIB 10280
1417	G. stearothermophilus NRS-19, 1503, DSM 2027, ATCC 7954, NCIB 8924, ...
1418	G. stearothermophilus 186, DSM 2313, NCIB 10278
1420	G. stearothermophilus N.R. Smith T17, 1518, DSM 5934, ATCC 7953, ...
1421	G. stearothermophilus T15, DSM 6790, ATCC 10149, WDCM 00069, CCUG ...
1444	G. stearothermophilus 10, DSM 13240, CIP 106956
1448	G. stearothermophilus C953, CCUG 34144, HNCMB 101025, LMG 11163, ...
100611	G. stearothermophilus STH00420(ZIMET), CMI 98373, ACC 1829, HKI ...
134733	G. stearothermophilus CIP 57.16, NBIMCC 2329
142398	G. stearothermophilus CCUG 12317
149136	G. stearothermophilus CCUG 36912
151704	G. stearothermophilus CCUG 44677
154550	G. stearothermophilus CCUG 54374
156003	G. stearothermophilus CCUG 59982
161282	G. stearothermophilus JCM 12894
163708	G. stearothermophilus JCM 30104

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
221	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
221	positive	growth	55

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125438		92.89

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body Product	#Plant	#Juice (natural)

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
221	sugar beet juice from extraction installations	Austria	AUT	Europe

Taxonmaps

69479

Global distribution of 16S sequence FN428681 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	603
Soil Samples	387
Animal Samples	2094
Plant Samples	132
Total Samples	3216

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
221	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM230013v1 assembly for Geobacillus stearothermophilus DSM 458	complete	GCA_002300135	1422.37	2842059332	1422	73.41

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
221	Geobacillus stearothermophilus partial 16S rRNA gene, strain R-32765	FN428681	1511		1422

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Geobacillus stearothermophilus strain DSM 458
PATRIC: 1422.37

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	87.10	no
125439	motility	BacteriaNetⓘ	yes	80.80	no
125439	gram_stain	BacteriaNetⓘ	variable	84.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	58.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Geobacillus stearothermophilus DSM 458
NCBI: GCA_002300135.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	77.25	no
125438	anaerobic	anaerobicⓘ	no	94.81	no
125438	aerobic	aerobicⓘ	yes	77.84	no
125438	spore-forming	spore-formingⓘ	yes	92.89	no
125438	thermophilic	thermophileⓘ	yes	70.25	yes
125438	flagellated	motile2+ⓘ	yes	82.22	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain Geobacillus stearothermophilus H6.	Lai R, Lin M, Yan Y, Jiang S, Zhou Z, Wang J.	Genes (Basel)	10.3390/genes14020466	2023
	Identification of a New Heavy-Metal-Resistant Strain of Geobacillus stearothermophilus Isolated from a Hydrothermally Active Volcanic Area in Southern Italy.	Puopolo R, Gallo G, Mormone A, Limauro D, Contursi P, Piochi M, Bartolucci S, Fiorentino G.	Int J Environ Res Public Health	10.3390/ijerph17082678	2020
Enzymology	Thermophilic Geobacillus WSUCF1 Secretome for Saccharification of Ammonia Fiber Expansion and Extractive Ammonia Pretreated Corn Stover.	Bhalla A, Arce J, Ubanwa B, Singh G, Sani RK, Balan V.	Front Microbiol	10.3389/fmicb.2022.844287	2022
Transcriptome	Genomic and Transcriptome Analyses of a Thermophilic Bacterium Geobacillus stearothermophilus B5 Isolated from Compost Reveal Its Enzymatic Basis for Lignocellulose Degradation.	Wang M, Miao J, Wang X, Li T, Zhu H, Liu D, Shen Q.	Microorganisms	10.3390/microorganisms8091357	2020
	Genomic identification of germinant receptors and phenotypic response to germinants in Parageobacillus and Geobacillus spp. strains.	Salvador M, Yruela I, Condon S, Gayan E.	Curr Res Microb Sci	10.1016/j.crmicr.2025.100461	2025
	Transposon-encoded nucleases use guide RNAs to promote their selfish spread.	Meers C, Le HC, Pesari SR, Hoffmann FT, Walker MWG, Gezelle J, Tang S, Sternberg SH.	Nature	10.1038/s41586-023-06597-1	2023
	Microbiome-based environmental monitoring of a dairy processing facility highlights the challenges associated with low microbial-load samples.	McHugh AJ, Yap M, Crispie F, Feehily C, Hill C, Cotter PD.	NPJ Sci Food	10.1038/s41538-021-00087-2	2021
Phylogeny	Bioinformatic prospecting and phylogenetic analysis reveals 94 undescribed circular bacteriocins and key motifs.	Vezina B, Rehm BHA, Smith AT.	BMC Microbiol	10.1186/s12866-020-01772-0	2020
Phylogeny	Discovery of thermophilic Bacillales using reduced-representation genotyping for identification.	Talamantes-Becerra B, Carling J, Kilian A, Georges A.	BMC Microbiol	10.1186/s12866-020-01800-z	2020
Phylogeny	Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment.	Schultz J, Parise MTD, Parise D, Medeiros LG, Sousa TJ, Kato RB, Uetanabaro APT, Araujo F, Ramos RTJ, de Castro Soares S, Brenig B, de Carvalho Azevedo VA, Goes-Neto A, Rosado AS.	Microorganisms	10.3390/microorganisms10081673	2022
Genetics	Pan-Genome Analyses of Geobacillus spp. Reveal Genetic Characteristics and Composting Potential.	Wang M, Zhu H, Kong Z, Li T, Ma L, Liu D, Shen Q.	Int J Mol Sci	10.3390/ijms21093393	2020
Metabolism	Cadmium ion biosorption by the thermophilic bacteria Geobacillus stearothermophilus and G. thermocatenulatus.	Hetzer A, Daughney CJ, Morgan HW.	Appl Environ Microbiol	10.1128/aem.00295-06	2006
Enzymology	Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression.	Heydari M, Ohshima T, Nunoura-Kominato N, Sakuraba H.	Appl Environ Microbiol	10.1128/aem.70.2.937-942.2004	2004
Genetics	Genome Sequence of Geobacillus stearothermophilus DSM 458, an Antimicrobial-Producing Thermophilic Bacterium, Isolated from a Sugar Beet Factory.	Egan K, Kelleher P, Field D, Rea MC, Ross RP, Cotter PD, Hill C	Genome Announc	10.1128/genomeA.01172-17	2017

References

#221	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 458
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Geobacillus stearothermophilus (DSM 458, R-32765, S 65-67)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002300135

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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