Geobacillus stearothermophilus T15 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from Food, Milk.
spore-forming Gram-positive rod-shaped aerobe 16S sequence Bacteria
SI-ID 12230
| @ref 20215 |
|
Last LPSN update
2026-02-09 11:18:06 |
| Domain Bacteria |
| Phylum Bacillota |
| Class Bacilli |
| Order Caryophanales |
| Family Bacillaceae |
| Genus Geobacillus |
| Species Geobacillus stearothermophilus |
| Full scientific name Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001 |
| Synonyms (1) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 2818 | NUTRIENT AGAR (DSMZ Medium 1) | Medium recipe at MediaDive  | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water | ||
| 41829 | TCS-IP-Boites | ||||
| 123300 | CIP Medium 3 | Medium recipe at CIP | |||
| 123300 | CIP Medium 72 | Medium recipe at CIP |
| @ref | Ability | Type | PH | |
|---|---|---|---|---|
| 123300 | positive | growth | 6 |
| Test 1 | Test 2 | Test 3 | |
|---|---|---|---|
| @ref | 2818 | 2818 | 2818 |
| Medium | Mueller-Hinton Agar | Mueller-Hinton Agar | Mueller-Hinton Agar |
| Manual annotation | 1 | 1 | 1 |
| Inhibition zone diameter in mm | Inhibition zone diameter in mm | Inhibition zone diameter in mm | |
| Amikacin 30µg (disc) | 32 | 32 | 40 |
| Ampicillin 10µg (disc) | 50 | 50 | >50 |
| Aztreonam 30µg (disc) | 26-28 | 26-28 | 40 |
| Bacitracin 10Unit | 32 | 32 | 42-44 |
| Cefalotin 30µg (disc) | >50 | >50 | >50 |
| Cefazolin 30µg (disc) | 50 | 50 | >50 |
| Cefotaxime 30µg (disc) | 48 | 48 | >50 |
| Ceftriaxone 30µg (disc) | 42 | 42 | >50 |
| Chloramphenicol 30µg (disc) | 30 | 30 | 34-36 |
| Clindamycin 10µg (disc) | 38 | 38 | >50 |
| Colistin 10µg (disc) | 14 | 14 | 24 |
| Doxycycline 30µg (disc) | 38-40 | 38-40 | 46 |
| Erythromycin 15µg (disc) | 30 | 30 | 40 |
| Fosfomycin 50µg (disc) | 34-36 | 34-36 | 40 |
| Gentamycin 10µg (disc) | |||
| Imipenem 10µg (disc) | >50 | >50 | >50 |
| Kanamycin 30µg (disc) | 28 | 28 | 34 |
| Lincomycin 15µg (disc) | 32 | 32 | 50 |
| Linezolid 10µg (disc) | 32 | 32 | 38 |
| Mezlocillin 30µg (disc) | 50 | 50 | >50 |
| Moxifloxacin 5µg (disc) | 32 | 32 | 40 |
| Neomycin 30µg (disc) | 28 | 28 | 32-34 |
| Nitrofurantoin 100µg (disc) | 30 | 30 | 40 |
| Norfloxacin 10µg (disc) | 28-30 | 28-30 | 38 |
| Nystatin 100Unit | 6-8 | 6-8 | 0 |
| Ofloxacin 5µg (disc) | 30 | 30 | 36-38 |
| Oxacillin 5µg (disc) | 44-46 | 44-46 | >50 |
| Penicillin G 6µg (disc) | >50 | >50 | >50 |
| Pipemidic acid 20µg (disc) | 10-12 | 10-12 | 16 |
| Piperacillin/Tazobactam 40µg (disc) | 48-50 | 48-50 | >50 |
| Polymyxin B 300Unit | 18 | 18 | 22 |
| Quinupristin/Dalfopristin 15µg (disc) | 30 | 30 | 40 |
| Teicoplanin 30µg (disc) | 30 | 30 | 30 |
| Tetracycline 30µg (disc) | 40 | 40 | 42-44 |
| Ticarcillin 75µg (disc) | >50 | >50 | >50 |
| Vancomycin 30µg (disc) | 30 | 30 | 28-30 |
| 123300 | Spore formationyes |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 123300 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | - | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | + | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 123300 | amylase | + | ||
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 123300 | beta-galactosidase | - | 3.2.1.23 | |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 123300 | caseinase | - | 3.4.21.50 | |
| 123300 | catalase | - | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 123300 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 123300 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 123300 | gelatinase | +/- | ||
| 123300 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 123300 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 123300 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 123300 | ornithine decarboxylase | - | 4.1.1.17 | |
| 123300 | oxidase | + | ||
| 123300 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 123300 | tryptophan deaminase | - | ||
| 123300 | tween esterase | - | ||
| 123300 | urease | - | 3.5.1.5 | |
| 68382 | valine arylamidase | - | from API zym |
Metadata FA analysis  | |||||||||||||||||||||||||||||||
| @ref | 44239 | ||||||||||||||||||||||||||||||
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| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 124043 | Geobacillus stearothermophilus strain ATCC 10149 16S ribosomal RNA gene, partial sequence. | MH173795 | 928 |  | 1422 | |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Direct fermentation of potato starch and potato residues to lactic acid by Geobacillus stearothermophilus under non-sterile conditions. | Smerilli M, Neureiter M, Wurz S, Haas C, Fruhauf S, Fuchs W. | J Chem Technol Biotechnol | 10.1002/jctb.4627 | 2015 | | |
| Metabolism | Cadmium ion biosorption by the thermophilic bacteria Geobacillus stearothermophilus and G. thermocatenulatus. | Hetzer A, Daughney CJ, Morgan HW. | Appl Environ Microbiol | 10.1128/aem.00295-06 | 2006 | |
| Characterization of Clostridium perfringens Phage Endolysin PlyDolk21. | Seo S, Son B, Kong M. | Antibiotics (Basel) | 10.3390/antibiotics14010081 | 2025 | | |
| LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1. | Choi D, Kong M. | Front Microbiol | 10.3389/fmicb.2023.1178748 | 2023 | | |
| Genomic identification of germinant receptors and phenotypic response to germinants in Parageobacillus and Geobacillus spp. strains. | Salvador M, Yruela I, Condon S, Gayan E. | Curr Res Microb Sci | 10.1016/j.crmicr.2025.100461 | 2025 | | |
| Isolation and Characterization of a Bacillus amyloliquefaciens Bacteriophage JBA6 and Its Endolysin PlyJBA6. | Kim J, Kong M. | J Microbiol Biotechnol | 10.4014/jmb.2502.02026 | 2025 | | |
| Enzymology | Comparison of pulsed light inactivation kinetics and modeling of Escherichia coli (ATCC-29055), Clostridium sporogenes (ATCC-7955) and Geobacillus stearothermophilus (ATCC-10149). | John D, Ramaswamy HS. | Curr Res Food Sci | 10.1016/j.crfs.2020.03.005 | 2020 | |
| Investigating Bio-Inspired Degradation of Toxic Dyes Using Potential Multi-Enzyme Producing Extremophiles. | Pham VHT, Kim J, Chang S, Bang D. | Microorganisms | 10.3390/microorganisms11051273 | 2023 | | |
| Isolation and Characterization of a Weizmannia coagulans Bacteriophage Youna2 and Its Endolysin PlyYouna2. | Son B, Kim Y, Yu B, Kong M. | J Microbiol Biotechnol | 10.4014/jmb.2303.03021 | 2023 | | |
| Enzymology | Adaptive Response of Thermophiles to Redox Stress and Their Role in the Process of dye Degradation From Textile Industry Wastewater. | Aragaw TA, Bogale FM, Gessesse A. | Front Physiol | 10.3389/fphys.2022.908370 | 2022 | |
| Bacteriophages of Thermophilic 'Bacillus Group' Bacteria-A Systematic Review, 2023 Update. | Skowron PM, Lubkowska B, Sobolewski I, Zylicz-Stachula A, Simoliuniene M, Simoliunas E. | Int J Mol Sci | 10.3390/ijms25063125 | 2024 | | |
| Rice Husk-Cellulose-Based Agricultural Waste Enhances the Degradation of Synthetic Dyes Using Multiple Enzyme-Producing Extremophiles. | Pham VHT, Kim J, Chang S, Shim J, Chung W, Bang D. | Microorganisms | 10.3390/microorganisms11081974 | 2023 | | |
| Screening Methods for Antimicrobial Residues in the Dairy Chain-The Past and the Present. | Navratilova P, Vorlova L, Dluhosova S, Bartakova K, Hanus O, Samkova E. | Antibiotics (Basel) | 10.3390/antibiotics13111098 | 2024 | | |
| Inactivation kinetics of Geobacillus stearothermophilus spores in water using high-pressure processing at elevated temperatures | Patazca E, Koutchma T, Ramaswamy HS. | J Food Sci | 2006 | | ||
| Stress | Strong and consistently synergistic inactivation of spores of spoilage-associated Bacillus and Geobacillus spp. by high pressure and heat compared with inactivation by heat alone. | Olivier SA, Bull MK, Stone G, van Diepenbeek RJ, Kormelink F, Jacops L, Chapman B. | Appl Environ Microbiol | 10.1128/aem.01957-10 | 2011 | |
| Enzymology | Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples. | Blake MR, Weimer BC. | Appl Environ Microbiol | 10.1128/aem.63.5.1643-1646.1997 | 1997 | |
| Gardimycin, a new antibiotic inhibiting peptidoglycan synthesis. | Somma S, Merati W, Parenti F. | Antimicrob Agents Chemother | 10.1128/aac.11.3.396 | 1977 | | |
| Metabolism | Purification and some properties of riboflavin synthetase from Bacillus stearothermophilus ATCC 8005. | Suzuki Y, Terai Y, Abe S. | Appl Environ Microbiol | 10.1128/aem.35.2.258-263.1978 | 1978 | |
| Identification of Homologous Polyprenols from Thermophilic Bacteria. | Gharwalova L, Palyzova A, Maresova H, Kolouchova I, Kyselova L, Rezanka T. | Microorganisms | 10.3390/microorganisms9061168 | 2021 | | |
| Analysis of Bacteriohopanoids from Thermophilic Bacteria by Liquid Chromatography-Mass Spectrometry. | Kolouchova I, Timkina E, Matatkova O, Kyselova L, Rezanka T. | Microorganisms | 10.3390/microorganisms9102062 | 2021 | | |
| Metabolism | Hyperstabilization of a thermophile bacterial laccase and its application for industrial dyes degradation. | Gianolini JE, Britos CN, Mulreedy CB, Trelles JA | 3 Biotech | 10.1007/s13205-020-02277-3 | 2020 | |
| Enzymology | Detection of maduramycin residues in the tissues of chickens and pheasants by the screening test for antibiotic residues (STAR). | Kozarova I, Macanga J, Goldova M, Major P, Tkacikova S | Food Addit Contam Part A Chem Anal Control Expo Risk Assess | 10.1080/19440049.2011.561879 | 2011 | |
| Biotechnology | Effects of superheated steam on Geobacillus stearothermophilus spore viability. | Head DS, Cenkowski S, Holley R, Blank G | J Appl Microbiol | 10.1111/j.1365-2672.2007.03647.x | 2007 | |
| Pathogenicity | Morpho-structural variations of bacterial spores after treatment in steam vacuum assisted autoclave. | Fonzi M, Montomoli E, Gasparini R, Devanna D, Fonzi L | Bull Group Int Rech Sci Stomatol Odontol | 10.3201/eid0801.010143 | 1999 | |
| Pathogenicity | Detection of penicillin G in milk using a conductimetric method. | Chen HC, Chang TC | J Dairy Sci | 10.3168/jds.S0022-0302(94)77091-0 | 1994 | |
| Comparison of Microbiological Methods for Detecting Penicillin and Penicillin-Like Antibiotic Residues in Milk. | Geleta JN, Bryant HH, Heavner JE, Colaianne JJ, Carter GG, Norcross MA | J Food Prot | 10.4315/0362-028X-47.8.604 | 1984 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive1421.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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