Name: Geobacillus stearothermophilus T15
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1421

Type strain:

Species: Geobacillus stearothermophilus

Strain Designation: T15

Culture col. no.: DSM 6790, ATCC 10149, WDCM 00069, CCUG 620, JCM 11297, CGMCC 1.6483, CIP 105453, IAM 11004, CCM 5965, LMG 17962, CECT 4517

Strain history: CIP <- 1998, ATCC <- N.R. Smith: strain T15 <- 1944, Curran, USDA <- Iowa State College, USA, Bacillus calidolactis.

NCBI tax ID(s): 1422 (species)

Other strains from Geobacillus stearothermophilus

Section

Geobacillus stearothermophilus T15 is an aerobe, spore-forming, thermophilic bacterium that was isolated from Food, Milk.

Gram-positive
rod-shaped
spore-forming
aerobe
thermophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Geobacillus*
	Species	**Geobacillus stearothermophilus**
	Full Scientific Name (LPSN)	Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

Synonym

Bacillus stearothermophilus

Information on morphological and physiological properties Morphology

[Ref.: #123300]	Gram stain	positive

[Ref.: #123300]	Cell shape	rod-shaped

[Ref.: #123300]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2818]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2818]

Culture medium growth

[Ref.: #2818]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2818]

Culture medium composition

expand / minimize

[Ref.: #41829]

Culture medium

TCS-IP-Boites

[Ref.: #41829]

Culture medium growth

[Ref.: #123300]

Culture medium

CIP Medium 3

[Ref.: #123300]

Culture medium growth

[Ref.: #123300]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123300]

Culture medium

CIP Medium 72

[Ref.: #123300]

Culture medium growth

[Ref.: #123300]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2818]		growth	55 °C
[Ref.: #41829]		growth	55 °C
[Ref.: #44239]	no	growth	42 °C
[Ref.: #44239]		growth	56 °C
[Ref.: #67770]		growth	55 °C
[Ref.: #123300]	no	growth	30 °C
[Ref.: #123300]	no	growth	25 °C
[Ref.: #123300]	no	growth	10 °C
[Ref.: #123300]		growth	41-55 °C
[Ref.: #123300]	no	growth	37 °C

[Ref.: #2818]	Temperature range	thermophilic
[Ref.: #41829]	Temperature range	thermophilic
[Ref.: #44239]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #123300]	Temperature range	mesophilic
[Ref.: #123300]	Temperature range	psychrophilic
[Ref.: #123300]	Temperature range	thermophilic
[Ref.: #123300]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #123300]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44239]	Oxygen tolerance	aerobe
[Ref.: #123300]	Oxygen tolerance	facultative anaerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Reference

[Ref.: #2818]
Mueller-Hinton Agar
55

aerob
yes
Inhibition zone diameter in mm
>50
44-46
50
>50
50
>50
50
48
26-28
>50
40
30
30
32
30
30
32
30
28-30
14
10-12
30
32
18
28
28
38-40
42
38
34-36
32
32
6-8
30
30
48-50

[Ref.: #2818]
Mueller-Hinton Agar
55

aerob
yes
Inhibition zone diameter in mm
>50
44-46
50
>50
50
>50
50
48
26-28
>50
40
30
30
32
30
30
32
30
28-30
14
10-12
30
32
18
28
28
38-40
42
38
34-36
32
32
6-8
30
30
48-50

Only first 15 entries are displayed. Click here to see all.

[Ref.: #123300]

Ability of spore formation

yes

	Salt	Tested relation		Salt conc.
[Ref.: #123300]	NaCl		growth	0-2	%
[Ref.: #123300]	NaCl	no	growth	4	%
[Ref.: #123300]	NaCl	no	growth	6	%
[Ref.: #123300]	NaCl	no	growth	8	%
[Ref.: #123300]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123300]	citrate ChEBI	-	carbon source
[Ref.: #123300]	esculin ChEBI	+	hydrolysis
[Ref.: #123300]	hippurate ChEBI	+	hydrolysis
[Ref.: #123300]	nitrate ChEBI	+	reduction
[Ref.: #123300]	nitrate ChEBI	+	respiration
[Ref.: #123300]	nitrite ChEBI	+	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123300]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123300]		acetoin ChEBI	-
[Ref.: #123300]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123300]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123300]	amylase	+
[Ref.: #123300]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123300]	caseinase	-	3.4.21.50
[Ref.: #123300]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #123300]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123300]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #123300]	gelatinase	+/-
[Ref.: #123300]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #123300]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123300]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123300]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123300]	oxidase	+
[Ref.: #123300]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123300]	tryptophan deaminase	-
[Ref.: #123300]	tween esterase	-
[Ref.: #123300]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44239]

Fatty acid	Percentage	ECL
C15:0 ISO	28	14.621
C16:0	21.8	16
C17:0 anteiso	18.3	16.722
C17:0 iso	14	16.629
C16:0 iso	8.2	15.626
C15:0 ANTEISO	6.4	14.711
C14:0	1.3	14
C17:0	1.1	17
C18:0	1	18

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123300]	8711	-	-	+	+	-	+	-	-	-	-	+	+	+	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #123300]	Sample type/isolated from	Food, Milk
[Ref.: #123300]	Geographic location (country and/or sea, region)	Collected before 1928
[Ref.: #123300]	Country	United States of America
[Ref.: #123300]	Country ISO 3 Code	USA
[Ref.: #123300]	Continent	North America
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2818]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123300]	Biosafety level	1	Risk group (French classification)

Availability in culture collections External links

[Ref.: #2818]

Culture collection no.

DSM 6790, ATCC 10149, WDCM 00069, CCUG 620, JCM 11297, CGMCC 1.6483, CIP 105453, IAM 11004, CCM 5965, LMG 17962, CECT 4517

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Hyperstabilization of a thermophile bacterial laccase and its application for industrial dyes degradation.	Gianolini JE, Britos CN, Mulreedy CB, Trelles JA	3 Biotech	10.1007/s13205-020-02277-3	2020	*
Enzymology	Detection of maduramycin residues in the tissues of chickens and pheasants by the screening test for antibiotic residues (STAR).	Kozarova I, Macanga J, Goldova M, Major P, Tkacikova S	Food Addit Contam Part A Chem Anal Control Expo Risk Assess	10.1080/19440049.2011.561879	2011	*
Biotechnology	Effects of superheated steam on Geobacillus stearothermophilus spore viability.	Head DS, Cenkowski S, Holley R, Blank G	J Appl Microbiol	10.1111/j.1365-2672.2007.03647.x	2007	*
Pathogenicity	Morpho-structural variations of bacterial spores after treatment in steam vacuum assisted autoclave.	Fonzi M, Montomoli E, Gasparini R, Devanna D, Fonzi L	Bull Group Int Rech Sci Stomatol Odontol	10.3201/eid0801.010143	1999	*
Pathogenicity	Detection of penicillin G in milk using a conductimetric method.	Chen HC, Chang TC	J Dairy Sci	10.3168/jds.S0022-0302(94)77091-0	1994	*
	Comparison of Microbiological Methods for Detecting Penicillin and Penicillin-Like Antibiotic Residues in Milk.	Geleta JN, Bryant HH, Heavner JE, Colaianne JJ, Carter GG, Norcross MA	J Food Prot	10.4315/0362-028X-47.8.604	1984	*

References

#2818

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6790

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41829 ; Curators of the CIP;
#44239 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 620
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#123300 Collection of Institut Pasteur ; Curators of the CIP; CIP 105453
* These data were automatically processed and therefore are not curated

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