Geobacillus stearothermophilus R-35646 is a facultative anaerobe, chemoorganotroph, spore-forming prokaryote that forms circular colonies and was isolated from deteriorated canned food.
spore-forming motile rod-shaped colony-forming facultative anaerobe chemoorganotroph thermophilic genome sequence 16S sequence| @ref 20215 |
|
|
| Domain Bacillati |
| Phylum Bacillota |
| Class Bacilli |
| Order Caryophanales |
| Family Bacillaceae |
| Genus Geobacillus |
| Species Geobacillus stearothermophilus |
| Full scientific name Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001 |
| Synonyms (1) |
| @ref | Colony size | Colony shape | Incubation period | Medium used | |
|---|---|---|---|---|---|
| 23042 | 0.5-2.0 mm | circular | 1-2 days | trypticase soy agar |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 23042 | trypticase soy agar | ||||
| 2023 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | Medium recipe at MediaDive | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water | ||
| 36276 | MEDIUM 29- Brain heart agar | Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g) | |||
| 123299 | CIP Medium 3 | Medium recipe at CIP | |||
| 123299 | CIP Medium 29 | Medium recipe at CIP |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 2023 | positive | growth | 55 | thermophilic | |
| 22918 | positive | maximum | 60 | thermophilic | |
| 22918 | positive | minimum | 40 | thermophilic | |
| 23042 | positive | growth | 40.0-60.0 | thermophilic | |
| 23042 | maximum | 60.0-70.0 | thermophilic | ||
| 23042 | minimum | 30.0-45.0 | |||
| 23042 | thermophilic | ||||
| 36276 | positive | growth | 55 | thermophilic | |
| 48397 | positive | growth | 55 | thermophilic | |
| 67770 | positive | growth | 55 | thermophilic | |
| 67772 | positive | optimum | 55-65 | thermophilic | |
| 67772 | positive | minimum | 37 | mesophilic | |
| 67772 | positive | maximum | 75 | thermophilic | |
| 123299 | positive | growth | 45-55 | thermophilic | |
| 123299 | negative | growth | 10 | ||
| 123299 | negative | growth | 22 | ||
| 123299 | negative | growth | 30 | ||
| 123299 | negative | growth | 37 |
| 123299 | Oxygen tolerancefacultative anaerobe |
| 23042 | Typechemoorganotroph |
| 67770 | ObservationBiological indicator for moist heat sterilization (Terminal sterilization and sterilization indicators, Japanese Pharmacopoeia, 16 ed.) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 23042 | 16808 ChEBI | 2-dehydro-D-gluconate | - | builds acid from | |
| 23042 | 17426 ChEBI | 5-dehydro-D-gluconate | - | builds acid from | |
| 22918 | 27613 ChEBI | amygdalin | - | builds acid from | |
| 23042 | 27613 ChEBI | amygdalin | - | builds acid from | |
| 22918 | 18305 ChEBI | arbutin | - | builds acid from | |
| 23042 | 18305 ChEBI | arbutin | - | builds acid from | |
| 23042 | casein | + | hydrolysis | ||
| 22918 | 17057 ChEBI | cellobiose | - | builds acid from | |
| 23042 | 17057 ChEBI | cellobiose | - | builds acid from | |
| 23042 | 16947 ChEBI | citrate | - | carbon source | |
| 123299 | 16947 ChEBI | citrate | - | carbon source | |
| 23042 | 17108 ChEBI | D-arabinose | - | builds acid from | |
| 23042 | 18333 ChEBI | D-arabitol | - | builds acid from | |
| 23042 | 15824 ChEBI | D-fructose | - | builds gas from | |
| 23042 | 15824 ChEBI | D-fructose | + | builds acid from | |
| 23042 | 28847 ChEBI | D-fucose | - | builds acid from | |
| 23042 | 17634 ChEBI | D-glucose | - | builds gas from | |
| 23042 | 17634 ChEBI | D-glucose | + | builds acid from | |
| 23042 | 62318 ChEBI | D-lyxose | - | builds acid from | |
| 23042 | 16024 ChEBI | D-mannose | - | builds gas from | |
| 23042 | 16024 ChEBI | D-mannose | + | builds acid from | |
| 23042 | 16443 ChEBI | D-tagatose | - | builds acid from | |
| 22918 | 65327 ChEBI | D-xylose | - | builds acid from | |
| 23042 | 65327 ChEBI | D-xylose | - | builds acid from | |
| 23042 | 17113 ChEBI | erythritol | - | builds acid from | |
| 23042 | 4853 ChEBI | esculin | + | hydrolysis | |
| 123299 | 4853 ChEBI | esculin | - | hydrolysis | |
| 23042 | 16813 ChEBI | galactitol | - | builds acid from | |
| 22918 | 28260 ChEBI | galactose | +/- | builds acid from | |
| 23042 | 28260 ChEBI | galactose | - | builds gas from | |
| 23042 | 28260 ChEBI | galactose | + | builds acid from | |
| 22918 | 5291 ChEBI | gelatin | + | hydrolysis | |
| 23042 | 5291 ChEBI | gelatin | + | hydrolysis | |
| 22918 | 28066 ChEBI | gentiobiose | - | builds acid from | |
| 23042 | 28066 ChEBI | gentiobiose | - | builds acid from | |
| 23042 | 24265 ChEBI | gluconate | - | builds acid from | |
| 22918 | 17754 ChEBI | glycerol | +/- | builds acid from | |
| 23042 | 17754 ChEBI | glycerol | - | builds gas from | |
| 23042 | 17754 ChEBI | glycerol | + | builds acid from | |
| 22918 | 28087 ChEBI | glycogen | +/- | builds acid from | |
| 23042 | 28087 ChEBI | glycogen | - | builds gas from | |
| 23042 | 28087 ChEBI | glycogen | + | builds acid from | |
| 123299 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 23042 | 15443 ChEBI | inulin | + | builds acid from | |
| 22918 | 30849 ChEBI | L-arabinose | - | builds acid from | |
| 23042 | 30849 ChEBI | L-arabinose | - | builds acid from | |
| 23042 | 18403 ChEBI | L-arabitol | - | builds acid from | |
| 23042 | 18287 ChEBI | L-fucose | - | builds acid from | |
| 23042 | 17266 ChEBI | L-sorbose | - | builds acid from | |
| 23042 | 65328 ChEBI | L-xylose | - | builds acid from | |
| 22918 | 17716 ChEBI | lactose | - | builds acid from | |
| 23042 | 17716 ChEBI | lactose | - | builds acid from | |
| 23042 | 17306 ChEBI | maltose | - | builds gas from | |
| 23042 | 17306 ChEBI | maltose | + | builds acid from | |
| 22918 | 29864 ChEBI | mannitol | - | builds acid from | |
| 23042 | 29864 ChEBI | mannitol | - | builds acid from | |
| 22918 | 6731 ChEBI | melezitose | + | builds acid from | |
| 23042 | 6731 ChEBI | melezitose | - | builds gas from | |
| 23042 | 6731 ChEBI | melezitose | + | builds acid from | |
| 22918 | 28053 ChEBI | melibiose | + | builds acid from | |
| 23042 | 28053 ChEBI | melibiose | - | builds gas from | |
| 23042 | 28053 ChEBI | melibiose | + | builds acid from | |
| 22918 | 37657 ChEBI | methyl D-glucoside | + | builds acid from | |
| 23042 | 37657 ChEBI | methyl D-glucoside | - | builds gas from | |
| 23042 | 37657 ChEBI | methyl D-glucoside | + | builds acid from | |
| 22918 | 17268 ChEBI | myo-inositol | - | builds acid from | |
| 23042 | 17268 ChEBI | myo-inositol | - | builds acid from | |
| 22918 | 506227 ChEBI | N-acetyl-D-glucosamine | - | builds acid from | |
| 23042 | 506227 ChEBI | N-acetylglucosamine | - | builds acid from | |
| 23042 | 17632 ChEBI | nitrate | + | reduction | |
| 123299 | 17632 ChEBI | nitrate | + | reduction | |
| 123299 | 17632 ChEBI | nitrate | + | respiration | |
| 123299 | 16301 ChEBI | nitrite | + | reduction | |
| 22918 | O-nitrophenyl-beta-D-galactopyranosid | - | hydrolysis | ||
| 23042 | O-nitrophenyl-beta-D-galactopyranosid | - | hydrolysis | ||
| 22918 | 16634 ChEBI | raffinose | + | builds acid from | |
| 23042 | 16634 ChEBI | raffinose | - | builds gas from | |
| 23042 | 16634 ChEBI | raffinose | + | builds acid from | |
| 23042 | 26546 ChEBI | rhamnose | - | builds acid from | |
| 23042 | 15963 ChEBI | ribitol | - | builds acid from | |
| 22918 | 33942 ChEBI | ribose | - | builds acid from | |
| 23042 | 33942 ChEBI | ribose | - | builds acid from | |
| 22918 | 17814 ChEBI | salicin | - | builds acid from | |
| 23042 | 17814 ChEBI | salicin | - | builds acid from | |
| 23042 | 30911 ChEBI | sorbitol | - | builds acid from | |
| 22918 | 28017 ChEBI | starch | + | hydrolysis | |
| 23042 | 28017 ChEBI | starch | - | builds gas from | |
| 23042 | 28017 ChEBI | starch | + | builds acid from | |
| 23042 | 28017 ChEBI | starch | + | hydrolysis | |
| 22918 | 17992 ChEBI | sucrose | + | builds acid from | |
| 23042 | 17992 ChEBI | sucrose | - | builds gas from | |
| 23042 | 17992 ChEBI | sucrose | + | builds acid from | |
| 22918 | 27082 ChEBI | trehalose | + | builds acid from | |
| 23042 | 27082 ChEBI | trehalose | - | builds gas from | |
| 23042 | 27082 ChEBI | trehalose | + | builds acid from | |
| 23042 | 27897 ChEBI | tryptophan | - | energy source | |
| 22918 | 32528 ChEBI | turanose | + | builds acid from | |
| 23042 | 32528 ChEBI | turanose | - | builds gas from | |
| 23042 | 32528 ChEBI | turanose | + | builds acid from | |
| 23042 | 17151 ChEBI | xylitol | - | builds acid from |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 123299 | alcohol dehydrogenase | + | 1.1.1.1 | |
| 68382 | alkaline phosphatase | - | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | + | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | + | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 123299 | amylase | + | ||
| 23042 | arginine dihydrolase | - | 3.5.3.6 | |
| 22918 | beta-galactosidase | - | 3.2.1.23 | |
| 23042 | beta-galactosidase | - | 3.2.1.23 | |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 123299 | beta-galactosidase | - | 3.2.1.23 | |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 123299 | caseinase | + | 3.4.21.50 | |
| 22918 | catalase | + | 1.11.1.6 | |
| 23042 | catalase | + | 1.11.1.6 | |
| 123299 | catalase | - | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 22918 | cytochrome oxidase | - | 1.9.3.1 | |
| 23042 | cytochrome oxidase | - | 1.9.3.1 | |
| 123299 | DNase | + | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 123299 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 22918 | gelatinase | + | ||
| 123299 | gelatinase | +/- | ||
| 123299 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 123299 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 23042 | lysine decarboxylase | - | 4.1.1.18 | |
| 123299 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | - | from API zym | |
| 23042 | ornithine decarboxylase | - | 4.1.1.17 | |
| 123299 | ornithine decarboxylase | - | 4.1.1.17 | |
| 123299 | oxidase | + | ||
| 123299 | protease | - | ||
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 23042 | tryptophan deaminase | - | 4.1.99.1 | |
| 123299 | tween esterase | + | ||
| 23042 | urease | - | 3.5.1.5 | |
| 123299 | urease | - | 3.5.1.5 | |
| 68382 | valine arylamidase | - | from API zym |
| Metadata FA analysis | ||||||||||||||||||||||
| type of FA analysis | whole cell analysis | |||||||||||||||||||||
| method/protocol | CCUG | |||||||||||||||||||||
| @ref | 48397 | |||||||||||||||||||||
|
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| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Engineered | #Biodegradation | #Composting | |
| #Engineered | #Food production | #Bottled, canned, packed food |
Global distribution of 16S sequence LC177207 (>99% sequence identity) for Geobacillus from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 124043 | ASM3036961v1 assembly for Geobacillus stearothermophilus ATCC 12980 | complete | 1366047 | 97.27 | ||||
| 67770 | ASM127780v1 assembly for Geobacillus stearothermophilus ATCC 12980 | scaffold | 1366047 | 52.03 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Geobacillus stearothermophilus gene for 16S rRNA, partial sequence | AB271757 | 1485 | 1422 | ||
| 20218 | Bacillus stearothermophilus 16S rRNA gene, strain DSM 22T | AJ294817 | 1505 | 1422 | ||
| 20218 | Geobacillus stearothermophilus strain BGSC 9A20 16S ribosomal RNA gene, complete sequence | AY608928 | 1562 | 1422 | ||
| 20218 | Geobacillus stearothermophilus strain DSM 22 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence | EU157949 | 284 | 1422 | ||
| 20218 | Geobacillus stearothermophilus isolate Gst2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723631 | 628 | 1422 | ||
| 20218 | Geobacillus stearothermophilus isolate Gst6 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723632 | 628 | 1422 | ||
| 20218 | Geobacillus stearothermophilus isolate Gst39 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723633 | 502 | 1422 | ||
| 2023 | Geobacillus stearothermophilus partial 16S rRNA gene, strain R-35646 | FN428694 | 1511 | 1422 | ||
| 67770 | Geobacillus stearothermophilus gene for 16S ribosomal RNA, partial sequence, strain: JCM 2501 | LC177207 | 1481 | 1422 | ||
| 67770 | B.stearothermophilus 16S ribosomal RNA | X60640 | 1434 | 1422 | ||
| 124043 | Geobacillus stearothermophilus gene for 16S rRNA, partial sequence. | AB006937 | 279 | 1422 | ||
| 124043 | Bacillus stearothermophilus gene for 16S ribosomal RNA. | AB021196 | 1523 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608927 | 1562 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608929 | 1562 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608930 | 1562 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608931 | 1562 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608932 | 1562 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608933 | 1562 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608941 | 1561 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608942 | 1561 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608948 | 1560 | 1422 | ||
| 124043 | Bacillus sp. BGSC W9A92 16S ribosomal RNA gene, complete sequence. | AY608989 | 1562 | 1422 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | yes | 88.30 | no |
| 125439 | motility | BacteriaNetⓘ | yes | 81.50 | no |
| 125439 | gram_stain | BacteriaNetⓘ | variable | 84.10 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | aerobe | 71.00 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 76.18 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 94.93 | yes |
| 125438 | spore-forming | spore-formingⓘ | yes | 91.37 | yes |
| 125438 | aerobic | aerobicⓘ | no | 50.47 | yes |
| 125438 | thermophilic | thermophileⓘ | yes | 67.41 | no |
| 125438 | flagellated | motile2+ⓘ | yes | 80.83 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Enzymology | Functional Characterization, Genome Assembly, and Annotation of Geobacillus sp. G4 Isolated from a Geothermal Field in Tacna, Peru. | Ticona ARP, Santos KCR, Ramirez-Arua HE, Castellanos R, Silva JP, Hamann PRV, Noronha EF, Lopes FAC. | Microorganisms | 10.3390/microorganisms13061374 | 2025 | |
| Temperature-Dependent tRNA Modifications in Bacillales. | Hoffmann A, Lorenz C, Fallmann J, Wolff P, Lechner A, Betat H, Morl M, Stadler PF. | Int J Mol Sci | 10.3390/ijms25168823 | 2024 | ||
| Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose. | Bawn M, Subrizi F, Lye GJ, Sheppard TD, Hailes HC, Ward JM. | Data Brief | 10.1016/j.dib.2018.05.140 | 2018 | ||
| Direct fermentation of potato starch and potato residues to lactic acid by Geobacillus stearothermophilus under non-sterile conditions. | Smerilli M, Neureiter M, Wurz S, Haas C, Fruhauf S, Fuchs W. | J Chem Technol Biotechnol | 10.1002/jctb.4627 | 2015 | ||
| Cold adaptation of tRNA nucleotidyltransferases: A tradeoff in activity, stability and fidelity. | Ernst FGM, Erber L, Sammler J, Juhling F, Betat H, Morl M. | RNA Biol | 10.1080/15476286.2017.1391445 | 2018 | ||
| Draft Genome Sequence of Geobacillus stearothermophilus Strain K4E3_SPR_NPP, Isolated from Kasol Hot Spring, Himachal Pradesh, India. | Rodge S, Patil N. | Microbiol Resour Announc | 10.1128/mra.00194-22 | 2022 | ||
| LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1. | Choi D, Kong M. | Front Microbiol | 10.3389/fmicb.2023.1178748 | 2023 | ||
| Investigation of relative humidity distribution and its impact on disinfection using a combination of robotic fogger and hydrogen peroxide. | Sistla PK, Raju PK. | Infect Prev Pract | 10.1016/j.infpip.2025.100452 | 2025 | ||
| A comparison of the spore heat resistance of dairy isolates of Geobacillus stearothermophilus obtained using a CDC biofilm reactor and a sporulating medium | Kumar M, Flint S, Palmer J, Plieger P. | Int Dairy J | 10.1016/j.idairyj.2021.105000 | 2021 | ||
| Use of Hydrogen Peroxide Vapour for Microbiological Disinfection in Hospital Environments: A Review. | Ayub A, Cheong YK, Castro JC, Cumberlege O, Chrysanthou A. | Bioengineering (Basel) | 10.3390/bioengineering11030205 | 2024 | ||
| Assessment of dry-fogged hydrogen peroxide as an "untact" room disinfection automation system for rapid terminal decontamination of a single isolation room in a healthcare institution. | Suh JW, Kim JY, Sohn JW, Lee SE, Kim HJ, Chi WJ, Lee MN, Yoon YK. | Antimicrob Resist Infect Control | 10.1186/s13756-025-01613-7 | 2025 | ||
| High-efficiency chromosomal integrative amplification strategy for overexpressing alpha-amylase in Bacillus licheniformis. | Shen P, Niu D, Liu X, Tian K, Permaul K, Singh S, Mchunu NP, Wang Z. | J Ind Microbiol Biotechnol | 10.1093/jimb/kuac009 | 2022 | ||
| Development of Bacterial Spore Pouches as a Tool to Evaluate the Sterilization Efficiency-A Case Study with Microwave Sterilization Using Clostridium sporogenes and Geobacillus stearothermophilus. | Soni A, Smith J, Archer R, Gardner A, Tong K, Brightwell G. | Foods | 10.3390/foods9101342 | 2020 | ||
| Phylogeny | Insights into the Geobacillus stearothermophilus species based on phylogenomic principles. | Burgess SA, Flint SH, Lindsay D, Cox MP, Biggs PJ. | BMC Microbiol | 10.1186/s12866-017-1047-x | 2017 | |
| Genomic identification of germinant receptors and phenotypic response to germinants in Parageobacillus and Geobacillus spp. strains. | Salvador M, Yruela I, Condon S, Gayan E. | Curr Res Microb Sci | 10.1016/j.crmicr.2025.100461 | 2025 | ||
| Pathogenicity | Airborne Disinfection by Dry Fogging Efficiently Inactivates Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Mycobacteria, and Bacterial Spores and Shows Limitations of Commercial Spore Carriers. | Schinkothe J, Scheinemann HA, Diederich S, Freese H, Eschbaumer M, Teifke JP, Reiche S. | Appl Environ Microbiol | 10.1128/aem.02019-20 | 2021 | |
| Pathogenicity | Correlations between available primary amines, endospore coat thickness, and alkaline glutaraldehyde sensitivity for spores of select Bacillus species. | Player JK, Despain JT, Robison RA. | Microbiologyopen | 10.1002/mbo3.1117 | 2020 | |
| Phylogeny | Discovery of thermophilic Bacillales using reduced-representation genotyping for identification. | Talamantes-Becerra B, Carling J, Kilian A, Georges A. | BMC Microbiol | 10.1186/s12866-020-01800-z | 2020 | |
| Risk of Contamination in Assembled vs Disassembled Instruments in Hip Arthroplasty Surgery. | Mayer RR, Bederman SS, Colin VM, Berger MM, Cesario TC, Schwarzkopf R. | J Arthroplasty | 10.1016/j.arth.2016.02.004 | 2016 | ||
| Metabolism | Levels and Characteristics of mRNAs in Spores of Firmicute Species. | Byrd B, Camilleri E, Korza G, Craft DL, Green J, Rocha Granados M, Mok WWK, Caimano MJ, Setlow P. | J Bacteriol | 10.1128/jb.00017-21 | 2021 | |
| Targeting at the Nanoscale: A Novel S-Layer Fusion Protein Enabling Controlled Immobilization of Biotinylated Molecules. | Varga M. | Nanomaterials (Basel) | 10.3390/nano6110199 | 2016 | ||
| Determining the Effectiveness of Decontamination with Ionized Hydrogen Peroxide. | Grimaldo MA, Bouyer DH, de Siqueira CLM. | Appl Biosaf | 10.1177/1535676020935405 | 2020 | ||
| Validation of N95 Filtering Facepiece Respirator Decontamination Methods Available at a Large University Hospital. | Wigginton KR, Arts PJ, Clack HL, Fitzsimmons WJ, Gamba M, Harrison KR, LeBar W, Lauring AS, Li L, Roberts WW, Rockey NC, Torreblanca J, Young C, Anderegg LG, Cohn AM, Doyle JM, Meisenhelder CM, Raskin L, Love NG, Kaye KS. | Open Forum Infect Dis | 10.1093/ofid/ofaa610 | 2021 | ||
| The Hitchhiker's Guide to Hydrogen Peroxide Fumigation, Part 2: Verifying and Validating Hydrogen Peroxide Fumigation Cycles. | Kumin D, Albert MG, Weber B, Summermatter K. | Appl Biosaf | 10.1089/apb.21.921099 | 2021 | ||
| Pathogenicity | Suitability of different construction materials for use in aseptic processing environments decontaminated with gaseous hydrogen peroxide. | Unger B, Rauschnabel U, Duthorn B, Kottke V, Hertel C, Rauschnabel J. | PDA J Pharm Sci Technol | 2007 | ||
| Metabolism | Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium. | Cordova LT, Long CP, Venkataramanan KP, Antoniewicz MR. | Metab Eng | 10.1016/j.ymben.2015.09.009 | 2015 | |
| Pathogenicity | Use of a mixture of surrogates for infectious bioagents in a standard approach to assessing disinfection of environmental surfaces. | Sabbah S, Springthorpe S, Sattar SA. | Appl Environ Microbiol | 10.1128/aem.00246-10 | 2010 | |
| Rational Engineering of the Substrate Specificity of a Thermostable D-Hydantoinase (Dihydropyrimidinase). | Aganyants H, Weigel P, Hovhannisyan Y, Lecocq M, Koloyan H, Hambardzumyan A, Hovsepyan A, Hallet JN, Sakanyan V. | High Throughput | 10.3390/ht9010005 | 2020 | ||
| Metabolism | Biocatalytic synthesis of lactosucrose using a recombinant thermostable beta-fructofuranosidase from Arthrobacter sp. 10138. | Chen C, Deng J, Lv X, Li J, Du G, Li H, Liu L. | Bioengineered | 10.1080/21655979.2020.1739404 | 2020 | |
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| Characterizing the S-layer structure and anti-S-layer antibody recognition on intact Tannerella forsythia cells by scanning probe microscopy and small angle X-ray scattering. | Oh YJ, Sekot G, Duman M, Chtcheglova L, Messner P, Peterlik H, Schaffer C, Hinterdorfer P. | J Mol Recognit | 10.1002/jmr.2298 | 2013 | ||
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| Enzymology | Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression. | Heydari M, Ohshima T, Nunoura-Kominato N, Sakuraba H. | Appl Environ Microbiol | 10.1128/aem.70.2.937-942.2004 | 2004 | |
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| Influence of the incubation temperature and total dissolved solids concentration on the biofilm and spore formation of dairy isolates of Geobacillus stearothermophilus. | Kumar M, Flint S, Palmer J, Chanapha S, Hall C | Appl Environ Microbiol | 10.1128/AEM.02311-20 | 2021 | ||
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| Pathogenicity | A microbiological inhibition method for the rapid, broad-spectrum, and high-throughput screening of 34 antibiotic residues in milk. | Wu Q, Zhu Q, Liu Y, Shabbir MAB, Sattar A, Peng D, Tao Y, Chen D, Wang Y, Yuan Z | J Dairy Sci | 10.3168/jds.2019-16480 | 2019 | |
| Stress | The effect of phosphate on the heat resistance of spores of dairy isolates of Geobacillus stearothermophilus. | Kumar M, Flint SH, Palmer J, Plieger PG, Waterland M | Int J Food Microbiol | 10.1016/j.ijfoodmicro.2019.108334 | 2019 | |
| Enzymology | Heat resistance of spores of 18 strains of Geobacillus stearothermophilus and impact of culturing conditions. | Wells-Bennik MHJ, Janssen PWM, Klaus V, Yang C, Zwietering MH, Den Besten HMW | Int J Food Microbiol | 10.1016/j.ijfoodmicro.2018.11.005 | 2018 | |
| Stress | Walking dead: Permeabilization of heat-treated Geobacillus stearothermophilus ATCC 12980 spores under growth-preventing conditions. | Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert O | Food Microbiol | 10.1016/j.fm.2016.12.013 | 2016 | |
| Biotechnology | Die another day: Fate of heat-treated Geobacillus stearothermophilus ATCC 12980 spores during storage under growth-preventing conditions. | Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert O | Food Microbiol | 10.1016/j.fm.2015.12.007 | 2015 | |
| Stress | Modeling the behavior of Geobacillus stearothermophilus ATCC 12980 throughout its life cycle as vegetative cells or spores using growth boundaries. | Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert O | Food Microbiol | 10.1016/j.fm.2014.10.013 | 2014 | |
| Small-angle X-ray scattering for imaging of surface layers on intact bacteria in the native environment. | Sekot G, Schuster D, Messner P, Pum D, Peterlik H, Schaffer C | J Bacteriol | 10.1128/JB.02164-12 | 2013 | ||
| Metabolism | Crystallization of domains involved in self-assembly of the S-layer protein SbsC. | Ethordic A, Egelseer EM, Tesarz M, Sleytr UB, Keller W, Pavkov-Keller T | Acta Crystallogr Sect F Struct Biol Cryst Commun | 10.1107/S1744309112042650 | 2012 | |
| Towards the structure of the C-terminal part of the S-layer protein SbsC. | Kroutil M, Pavkov T, Birner-Gruenberger R, Tesarz M, Sleytr UB, Egelseer EM, Keller W | Acta Crystallogr Sect F Struct Biol Cryst Commun | 10.1107/S1744309109035386 | 2009 | ||
| Metabolism | The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions. | Ferner-Ortner-Bleckmann J, Huber-Gries C, Pavkov T, Keller W, Mader C, Ilk N, Sleytr UB, Egelseer EM | Mol Microbiol | 10.1111/j.1365-2958.2009.06734.x | 2009 | |
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| Pathogenicity | A novel approach to specific allergy treatment: the recombinant allergen-S-layer fusion protein rSbsC-Bet v 1 matures dendritic cells that prime Th0/Th1 and IL-10-producing regulatory T cells. | Gerstmayr M, Ilk N, Schabussova I, Jahn-Schmid B, Egelseer EM, Sleytr UB, Ebner C, Bohle B | J Immunol | 10.4049/jimmunol.179.11.7270 | 2007 | |
| Metabolism | High-affinity interaction between the S-layer protein SbsC and the secondary cell wall polymer of Geobacillus stearothermophilus ATCC 12980 determined by surface plasmon resonance technology. | Ferner-Ortner J, Mader C, Ilk N, Sleytr UB, Egelseer EM | J Bacteriol | 10.1128/JB.00294-07 | 2007 | |
| Metabolism | Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides. | Trelles JA, Valino AL, Runza V, Lewkowicz ES, Iribarren AM | Biotechnol Lett | 10.1007/s10529-005-5628-7 | 2005 | |
| Stress | A method of increasing test range and accuracy of bioindicators: Geobacillus stearothermophilus spores. | Lundahl G | PDA J Pharm Sci Technol | 2003 | ||
| Phylogeny | Genetic polymorphism by RAPD-PCR and phenotypic characteristics of isolated thermotolerant Bacillus strains from hot spring sources. | Hazem A, Manar A | New Microbiol | 2003 | ||
| Biotechnology | A RAPD-based comparison of thermophilic bacilli from milk powders. | Ronimus RS, Parker LE, Turner N, Poudel S, Ruckert A, Morgan HW | Int J Food Microbiol | 10.1016/s0168-1605(02)00480-4 | 2003 | |
| Enzymology | The purification and characterization of a Bacillus stearothermophilus methionine aminopeptidase (MetAP). | Chung JM, Chung IY, Lee YS | J Biochem Mol Biol | 10.5483/bmbrep.2002.35.2.228 | 2002 | |
| A recombinant bacterial cell surface (S-layer)-major birch pollen allergen-fusion protein (rSbsC/Bet v1) maintains the ability to self-assemble into regularly structured monomolecular lattices and the functionality of the allergen. | Breitwieser A, Egelseer EM, Moll D, Ilk N, Hotzy C, Bohle B, Ebner C, Sleytr UB, Sara M | Protein Eng | 10.1093/protein/15.3.243 | 2002 | ||
| Metabolism | Characterization of an S-layer glycoprotein produced in the course of S-layer variation of Bacillus stearothermophilus ATCC 12980 and sequencing and cloning of the sbsD gene encoding the protein moiety. | Egelseer EM, Danhorn T, Pleschberger M, Hotzy C, Sleytr UB, Sara M | Arch Microbiol | 10.1007/s00203-001-0363-5 | 2001 | |
| Metabolism | Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms. | Jarosch M, Egelseer EM, Huber C, Moll D, Mattanovich D, Sleytr UB, Sara M | Microbiology (Reading) | 10.1099/00221287-147-5-1353 | 2001 | |
| Metabolism | ISBst12, a novel type of insertion-sequence element causing loss of S-layer-gene expression in Bacillus stearothermophilus ATCC 12980. | Egelseer EM, Idris R, Jarosch M, Danhorn T, Sleytr UB, Sara M | Microbiology (Reading) | 10.1099/00221287-146-9-2175 | 2000 | |
| Metabolism | S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli. | Jarosch M, Egelseer EM, Mattanovich D, Sleytr UB, Sara M | Microbiology (Reading) | 10.1099/00221287-146-2-273 | 2000 | |
| Stress | Predictive model to describe the combined effect of pH and NaCl on apparent heat resistance of Bacillus stearothermophilus. | Periago PM, Fernandez PS, Salmeron MC, Martinez A | Int J Food Microbiol | 10.1016/s0168-1605(98)00111-1 | 1998 | |
| Metabolism | The S-layer proteins of two Bacillus stearothermophilus wild-type strains are bound via their N-terminal region to a secondary cell wall polymer of identical chemical composition. | Egelseer EM, Leitner K, Jarosch M, Hotzy C, Zayni S, Sleytr UB, Sara M | J Bacteriol | 10.1128/JB.180.6.1488-1495.1998 | 1998 | |
| Enzymology | A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980. | Li Y, Triccas JA, Ferenci T | Biochim Biophys Acta | 10.1016/s0167-4781(97)00103-6 | 1997 | |
| Thermal Resistance of Bacillus stearothermophilus Heated at High Temperatures in Different Substrates. | Rodrigo F, Fernandez PS, Rodrigo M, Ocio MJ, Martinez A | J Food Prot | 10.4315/0362-028X-60.2.144 | 1997 | ||
| Metabolism | Evidence that an N-terminal S-layer protein fragment triggers the release of a cell-associated high-molecular-weight amylase in Bacillus stearothermophilus ATCC 12980. | Egelseer EM, Schocher I, Sleytr UB, Sara M | J Bacteriol | 10.1128/jb.178.19.5602-5609.1996 | 1996 | |
| D Values of Bacillus stearothermophilus Spores as a Function of pH and Recovery Medium Acidulant. | Fernandez PS, Gomez FJ, Ocio MJ, Rodrigo M, Sanchez T, Martinez A | J Food Prot | 10.4315/0362-028X-58.6.628 | 1995 | ||
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| Pathogenicity | Rapid assay for tetracycline in premixes and mixed feeds. | Katz JM, Katz SE | J Assoc Off Anal Chem | 1984 | ||
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| #2023 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #22918 | Anna E. Dinsdale, Gillian Halket, An Coorevits, Anita Van Landschoot, Hans-Jürgen Busse, Paul De Vos, Niall A. Logan: Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus. IJSEM 61: 1802 - 1810 2011 ( DOI 10.1099/ijs.0.025445-0 , PubMed 20817844 ) |
| #23042 | An Coorevits,Anna E. Dinsdale,Gillian Halket,Liesbeth Lebbe,Paul De Vos,Anita Van Landschoot,Niall A. Logan: Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.. IJSEM 62: 1470 - 1485 2012 ( DOI 10.1099/ijs.0.030346-0 , PubMed 21856988 ) |
| #36276 | ; Curators of the CIP; |
| #48397 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 26241 |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #67772 | The University of Coimbra Bacteria Culture Collection (UCCCB) ; Curators of the UCCCB; |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #123299 | Collection of Institut Pasteur ; Curators of the CIP; CIP 66.23 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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If you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive1419.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data