Name: Geobacillus stearothermophilus R-35646
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1419

Type strain:

Species: Geobacillus stearothermophilus

Strain Designation: R-35646

Culture col. no.: DSM 22, ATCC 12980, CCM 2062, CCUG 26241, IAM 11062, LMG 6939, NBRC 12550, NCIMB 8923, NCTC 10339, VKM B-2231, IFO 12550, NCA 26, JCM 2501, BCRC 11092, CIP 66.23, KCTC 1665, KCTC 1752, NCFB 1768, NRRL B-1172, VKM B-510, VTT E-81128, UCCCB118

Strain history: ATCC <- NCA, 26

NCBI tax ID(s): 1366047 (strain), 1422 (species)

Other strains from Geobacillus stearothermophilus

Section

Geobacillus stearothermophilus R-35646 is a chemoorganotroph, spore-forming, thermophilic bacterium that forms circular colonies and was isolated from deteriorated canned food.

colony-forming
motile
rod-shaped
spore-forming
chemoorganotroph
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Geobacillus*
	Species	**Geobacillus stearothermophilus**
	Full Scientific Name (PNU)	Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

Synonym

Bacillus stearothermophilus

Information on morphological and physiological properties Morphology

[Ref.: #23042]	Cell length	2.0-3.5 µm
[Ref.: #23042]	Cell width	0.6-1.0 µm
[Ref.: #23042]	Cell shape	rod-shaped
[Ref.: #23042]	Motility	yes
[Ref.: #23042]	Flagellum arrangement	peritrichous

[Ref.: #23042]	Colony size	0.5-2.0 mm
[Ref.: #23042]	Colony shape	circular
[Ref.: #23042]	Incubation period	1-2 days
[Ref.: #23042]	Cultivation medium used	trypticase soy agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23042]

Culture medium

trypticase soy agar

[Ref.: #23042]

Culture medium growth

[Ref.: #2023]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #2023]

Culture medium growth

[Ref.: #2023]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #2023]

Culture medium composition

expand / minimize

[Ref.: #36276]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #36276]

Culture medium growth

[Ref.: #36276]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #2023]	growth	55 °C
[Ref.: #22918]	maximum	60 °C
[Ref.: #22918]	minimum	40 °C
[Ref.: #23042]	growth	40.0-60.0 °C
[Ref.: #23042]	maximum	60.0-70.0 °C
[Ref.: #23042]	minimum	30.0-45.0 °C
[Ref.: #36276]	growth	55 °C
[Ref.: #48397]	growth	55 °C
[Ref.: #67770]	growth	55 °C
[Ref.: #67772]	optimum	55-65 °C
[Ref.: #67772]	minimum	37 °C
[Ref.: #67772]	maximum	75 °C

[Ref.: #2023]	Temperature range	thermophilic
[Ref.: #22918]	Temperature range	thermophilic
[Ref.: #23042]	Temperature range	thermophilic
[Ref.: #36276]	Temperature range	thermophilic
[Ref.: #48397]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #67772]	Temperature range	thermophilic
[Ref.: #67772]	Temperature range	mesophilic
[Ref.: #67772]	Temperature range	thermophilic

	Kind of pH		pH
[Ref.: #67772]		maximum	7.5
[Ref.: #67772]		minimum	6.2
[Ref.: #22918]		growth	9
[Ref.: #22918]	no	growth	5
[Ref.: #23042]		growth	6.0
[Ref.: #23042]		growth	9.0
[Ref.: #23042]	no	growth	5.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23042]

Nutrition type

chemoorganotroph

[Ref.: #23042]	Spore description	ellipsoidal,subterminal and/or terminal,in sporangia
[Ref.: #23042]	Type of spore	spore
[Ref.: #23042]	Ability of spore formation	yes
[Ref.: #22918]	Ability of spore formation	yes

[Ref.: #2023]	Name of produced compound	restriction endonuclease BstPI
[Ref.: #2023]	Name of produced compound	aspartate transaminase
[Ref.: #2023]	Name of produced compound	phosphinothricin
[Ref.: #22918]	Name of produced compound	Acetoin

	Salt	Tested relation		Salt conc.
[Ref.: #22918]	NaCl		growth	1	%(w/v)
[Ref.: #22918]	NaCl	no	growth	5	%(w/v)
[Ref.: #23042]	NaCl		growth	1.0	%(w/v)
[Ref.: #23042]	NaCl	no	growth	5.0	%(w/v)

[Ref.: #67770]

Observation

Biological indicator for moist heat sterilization (Terminal sterilization and sterilization indicators, Japanese Pharmacopoeia, 16 ed.)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23042]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23042]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #22918]	amygdalin ChEBI	-	builds acid from
[Ref.: #23042]	amygdalin ChEBI	-	builds acid from
[Ref.: #22918]	arbutin ChEBI	-	builds acid from
[Ref.: #23042]	arbutin ChEBI	-	builds acid from
[Ref.: #23042]	casein	+	hydrolysis
[Ref.: #22918]	cellobiose ChEBI	-	builds acid from
[Ref.: #23042]	cellobiose ChEBI	-	builds acid from
[Ref.: #23042]	citrate ChEBI	-	carbon source
[Ref.: #23042]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23042]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23042]	D-fructose ChEBI	-	builds gas from
[Ref.: #23042]	D-fructose ChEBI	+	builds acid from
[Ref.: #23042]	D-fucose ChEBI	-	builds acid from
[Ref.: #23042]	D-glucose ChEBI	-	builds gas from
[Ref.: #23042]	D-glucose ChEBI	+	builds acid from
[Ref.: #23042]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23042]	D-mannose ChEBI	-	builds gas from
[Ref.: #23042]	D-mannose ChEBI	+	builds acid from
[Ref.: #23042]	D-tagatose ChEBI	-	builds acid from
[Ref.: #22918]	D-xylose ChEBI	-	builds acid from
[Ref.: #23042]	D-xylose ChEBI	-	builds acid from
[Ref.: #23042]	erythritol ChEBI	-	builds acid from
[Ref.: #23042]	esculin ChEBI	+	hydrolysis
[Ref.: #23042]	galactitol ChEBI	-	builds acid from
[Ref.: #22918]	galactose ChEBI	+/-	builds acid from
[Ref.: #23042]	galactose ChEBI	-	builds gas from
[Ref.: #23042]	galactose ChEBI	+	builds acid from
[Ref.: #22918]	gelatin ChEBI	+	hydrolysis
[Ref.: #23042]	gelatin ChEBI	+	hydrolysis
[Ref.: #22918]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23042]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23042]	gluconate ChEBI	-	builds acid from
[Ref.: #22918]	glycerol ChEBI	+/-	builds acid from
[Ref.: #23042]	glycerol ChEBI	-	builds gas from
[Ref.: #23042]	glycerol ChEBI	+	builds acid from
[Ref.: #22918]	glycogen ChEBI	+/-	builds acid from
[Ref.: #23042]	glycogen ChEBI	-	builds gas from
[Ref.: #23042]	glycogen ChEBI	+	builds acid from
[Ref.: #23042]	inulin ChEBI	+	builds acid from
[Ref.: #22918]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23042]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23042]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23042]	L-fucose ChEBI	-	builds acid from
[Ref.: #23042]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23042]	L-xylose ChEBI	-	builds acid from
[Ref.: #22918]	lactose ChEBI	-	builds acid from
[Ref.: #23042]	lactose ChEBI	-	builds acid from
[Ref.: #23042]	maltose ChEBI	-	builds gas from
[Ref.: #23042]	maltose ChEBI	+	builds acid from
[Ref.: #22918]	mannitol ChEBI	-	builds acid from
[Ref.: #23042]	mannitol ChEBI	-	builds acid from
[Ref.: #22918]	melezitose ChEBI	+	builds acid from
[Ref.: #23042]	melezitose ChEBI	-	builds gas from
[Ref.: #23042]	melezitose ChEBI	+	builds acid from
[Ref.: #22918]	melibiose ChEBI	+	builds acid from
[Ref.: #23042]	melibiose ChEBI	-	builds gas from
[Ref.: #23042]	melibiose ChEBI	+	builds acid from
[Ref.: #22918]	methyl D-glucoside ChEBI	+	builds acid from
[Ref.: #23042]	methyl D-glucoside ChEBI	-	builds gas from
[Ref.: #23042]	methyl D-glucoside ChEBI	+	builds acid from
[Ref.: #22918]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23042]	myo-inositol ChEBI	-	builds acid from
[Ref.: #22918]	N-acetyl-D-glucosamine ChEBI	-	builds acid from
[Ref.: #23042]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #23042]	nitrate ChEBI	+	reduction
[Ref.: #22918]	O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
[Ref.: #23042]	O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
[Ref.: #22918]	raffinose ChEBI	+	builds acid from
[Ref.: #23042]	raffinose ChEBI	-	builds gas from
[Ref.: #23042]	raffinose ChEBI	+	builds acid from
[Ref.: #23042]	rhamnose ChEBI	-	builds acid from
[Ref.: #23042]	ribitol ChEBI	-	builds acid from
[Ref.: #22918]	ribose ChEBI	-	builds acid from
[Ref.: #23042]	ribose ChEBI	-	builds acid from
[Ref.: #22918]	salicin ChEBI	-	builds acid from
[Ref.: #23042]	salicin ChEBI	-	builds acid from
[Ref.: #23042]	sorbitol ChEBI	-	builds acid from
[Ref.: #22918]	starch ChEBI	+	hydrolysis
[Ref.: #23042]	starch ChEBI	-	builds gas from
[Ref.: #23042]	starch ChEBI	+	builds acid from
[Ref.: #23042]	starch ChEBI	+	hydrolysis
[Ref.: #22918]	sucrose ChEBI	+	builds acid from
[Ref.: #23042]	sucrose ChEBI	-	builds gas from
[Ref.: #23042]	sucrose ChEBI	+	builds acid from
[Ref.: #22918]	trehalose ChEBI	+	builds acid from
[Ref.: #23042]	trehalose ChEBI	-	builds gas from
[Ref.: #23042]	trehalose ChEBI	+	builds acid from
[Ref.: #23042]	tryptophan ChEBI	-	energy source
[Ref.: #22918]	turanose ChEBI	+	builds acid from
[Ref.: #23042]	turanose ChEBI	-	builds gas from
[Ref.: #23042]	turanose ChEBI	+	builds acid from
[Ref.: #23042]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #22918]		acetoin ChEBI
[Ref.: #23042]		acetoin ChEBI
[Ref.: #23042]		hydrogen sulfide ChEBI
[Ref.: #23042]		indole ChEBI

	Metabolite	Voges-Proskauer-test	Indole test	Citrate test
[Ref.: #22918]	acetoin ChEBI	+
[Ref.: #23042]	acetoin ChEBI	+
[Ref.: #23042]	citrate ChEBI			-
[Ref.: #23042]	indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #23042]	arginine dihydrolase	-	3.5.3.6
[Ref.: #22918]	beta-galactosidase	-	3.2.1.23
[Ref.: #23042]	beta-galactosidase	-	3.2.1.23
[Ref.: #22918]	catalase	+	1.11.1.6
[Ref.: #23042]	catalase	+	1.11.1.6
[Ref.: #22918]	cytochrome oxidase	-	1.9.3.1
[Ref.: #23042]	cytochrome oxidase	-	1.9.3.1
[Ref.: #22918]	gelatinase	+
[Ref.: #23042]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23042]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23042]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23042]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #48397]

Fatty acid	Percentage	ECL
C15:0 ISO	36.8	14.621
C17:0 anteiso	23.4	16.722
C17:0 iso	13.2	16.629
C15:0 ANTEISO	10.9	14.711
C16:0	10.7	16
C16:0 iso	5	15.626

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2023]	Sample type/isolated from	deteriorated canned food

[Ref.: #67772]	Sample type/isolated from	Deteriorated canned food
[Ref.: #67772]	Sampling date	1990
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Engineered	#Food production	#Bottled, canned, packed food

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC177207 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	603
Soil Samples	387
Animal Samples	2094
Plant Samples	132
Total Samples	3216

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2023]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #67772]	Biosafety level	1

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Geobacillus stearothermophilus gene for 16S ribosomal RNA, partial sequence, strain: JCM 2501	LC177207	1481	ENA	1422 tax ID
[Ref.: #2023]	Geobacillus stearothermophilus partial 16S rRNA gene, strain R-35646	FN428694	1511	ENA	1422 tax ID
[Ref.: #67770]	B.stearothermophilus 16S ribosomal RNA	X60640	1434	ENA	1422 tax ID
[Ref.: #20218]	Bacillus stearothermophilus 16S rRNA gene, strain DSM 22T	AJ294817	1505	ENA	1422 tax ID	*
[Ref.: #20218]	Geobacillus stearothermophilus strain DSM 22 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence	EU157949	284	ENA	1422 tax ID	*
[Ref.: #20218]	Geobacillus stearothermophilus strain BGSC 9A20 16S ribosomal RNA gene, complete sequence	AY608928	1562	ENA	1422 tax ID	*
[Ref.: #20218]	Geobacillus stearothermophilus gene for 16S rRNA, partial sequence	AB271757	1485	ENA	1422 tax ID	*
[Ref.: #20218]	Geobacillus stearothermophilus clone EM-2_F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF478064	628	ENA	1366047 tax ID	*
[Ref.: #20218]	Geobacillus stearothermophilus isolate Gst2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723631	628	ENA	1422 tax ID	*
[Ref.: #20218]	Geobacillus stearothermophilus isolate Gst6 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723632	628	ENA	1422 tax ID	*
[Ref.: #20218]	Geobacillus stearothermophilus isolate Gst39 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723633	502	ENA	1422 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Geobacillus stearothermophilus ATCC 12980 ATCC 12980	GCA_001277805	scaffold	GenBank	1366047 tax ID
[Ref.: #66792]	Geobacillus stearothermophilus ATCC 12980	1366047.4	wgs	PATRIC	1366047 tax ID	*
[Ref.: #66792]	Geobacillus stearothermophilus ATCC 12980	2654587565	draft	JGI IMG/M	1366047 tax ID	*

[Ref.: #2023]	GC-content	52.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #2023]	GC-content	51.9 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #2023]	GC-content	51.5 mol%	Buoyant density centrifugation (BD)
[Ref.: #67770]	GC-content	51.6 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #2023]

Culture collection no.

DSM 22, ATCC 12980, CCM 2062, CCUG 26241, IAM 11062, LMG 6939, NBRC 12550, NCIMB 8923, NCTC 10339, VKM B-2231, IFO 12550, NCA 26, JCM 2501, BCRC 11092, CIP 66.23, KCTC 1665, KCTC 1752, NCFB 1768, NRRL B-1172, VKM B-510, VTT E-81128, UCCCB118

[Ref.: #20218]

Associated Passport(s) in StrainInfo

5108, 5103, 5105, 122549, 5109, 5102, 333130, 333130, 174902, 5119, 337663

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Performance evaluation of bactericidal effect and endotoxin inactivation by low-temperature ozone/hydrogen peroxide mixed gas exposure.	Nomura Y, Yamamura J, Fukui C, Fujimaki H, Sakamoto K, Matsuo KI, Kuromatsu H, Kikuchi Y, Haishima Y	J Biomed Mater Res B Appl Biomater	10.1002/jbm.b.34840	2021	*
Enzymology	Simultaneous production of alpha and beta amylase enzymes using separate gene bearing recombinant vectors in the same Escherichia coli cells.	Ozcan D, SIpahIoGlu HM	Turk J Biol	10.3906/biy-2001-71	2020	*
Cultivation	The search for a microbiological inhibition method for the rapid, broad-spectrum and high-throughput screening of six kinds of antibiotic residues in swine urine.	Wu Q, Zhu Q, Shabbir MAB, Sattar A, Peng D, Tao Y, Chen D, Yuan Z, Wang Y	Food Chem	10.1016/j.foodchem.2020.127580	2020	*
Pathogenicity	A microbiological inhibition method for the rapid, broad-spectrum, and high-throughput screening of 34 antibiotic residues in milk.	Wu Q, Zhu Q, Liu Y, Shabbir MAB, Sattar A, Peng D, Tao Y, Chen D, Wang Y, Yuan Z	J Dairy Sci	10.3168/jds.2019-16480	2019	*
Stress	The effect of phosphate on the heat resistance of spores of dairy isolates of Geobacillus stearothermophilus.	Kumar M, Flint SH, Palmer J, Plieger PG, Waterland M	Int J Food Microbiol	10.1016/j.ijfoodmicro.2019.108334	2019	*
Enzymology	Heat resistance of spores of 18 strains of Geobacillus stearothermophilus and impact of culturing conditions.	Wells-Bennik MHJ, Janssen PWM, Klaus V, Yang C, Zwietering MH, Den Besten HMW	Int J Food Microbiol	10.1016/j.ijfoodmicro.2018.11.005	2018	*
Stress	Walking dead: Permeabilization of heat-treated Geobacillus stearothermophilus ATCC 12980 spores under growth-preventing conditions.	Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert O	Food Microbiol	10.1016/j.fm.2016.12.013	2016	*
Biotechnology	Die another day: Fate of heat-treated Geobacillus stearothermophilus ATCC 12980 spores during storage under growth-preventing conditions.	Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert O	Food Microbiol	10.1016/j.fm.2015.12.007	2015	*
Stress	Modeling the behavior of Geobacillus stearothermophilus ATCC 12980 throughout its life cycle as vegetative cells or spores using growth boundaries.	Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert O	Food Microbiol	10.1016/j.fm.2014.10.013	2014	*
Metabolism	Crystallization of domains involved in self-assembly of the S-layer protein SbsC.	Ethordic A, Egelseer EM, Tesarz M, Sleytr UB, Keller W, Pavkov-Keller T	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/S1744309112042650	2012	*
Metabolism	The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions.	Ferner-Ortner-Bleckmann J, Huber-Gries C, Pavkov T, Keller W, Mader C, Ilk N, Sleytr UB, Egelseer EM	Mol Microbiol	10.1111/j.1365-2958.2009.06734.x	2009	*
Enzymology	Menaquinone reduction by an HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus.	Shibata H, Suzuki K, Kobayashi S	Can J Microbiol	10.1139/W07-077	2007	*
Pathogenicity	A novel approach to specific allergy treatment: the recombinant allergen-S-layer fusion protein rSbsC-Bet v 1 matures dendritic cells that prime Th0/Th1 and IL-10-producing regulatory T cells.	Gerstmayr M, Ilk N, Schabussova I, Jahn-Schmid B, Egelseer EM, Sleytr UB, Ebner C, Bohle B	J Immunol	10.4049/jimmunol.179.11.7270	2007	*
Metabolism	High-affinity interaction between the S-layer protein SbsC and the secondary cell wall polymer of Geobacillus stearothermophilus ATCC 12980 determined by surface plasmon resonance technology.	Ferner-Ortner J, Mader C, Ilk N, Sleytr UB, Egelseer EM	J Bacteriol	10.1128/JB.00294-07	2007	*
Metabolism	Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides.	Trelles JA, Valino AL, Runza V, Lewkowicz ES, Iribarren AM	Biotechnol Lett	10.1007/s10529-005-5628-7	2005	*
Stress	A method of increasing test range and accuracy of bioindicators: Geobacillus stearothermophilus spores.	Lundahl G	PDA J Pharm Sci Technol		2003	*
Phylogeny	Genetic polymorphism by RAPD-PCR and phenotypic characteristics of isolated thermotolerant Bacillus strains from hot spring sources.	Hazem A, Manar A	New Microbiol		2003	*
Biotechnology	A RAPD-based comparison of thermophilic bacilli from milk powders.	Ronimus RS, Parker LE, Turner N, Poudel S, Ruckert A, Morgan HW	Int J Food Microbiol	10.1016/s0168-1605(02)00480-4	2003	*
Enzymology	The purification and characterization of a Bacillus stearothermophilus methionine aminopeptidase (MetAP).	Chung JM, Chung IY, Lee YS	J Biochem Mol Biol	10.5483/bmbrep.2002.35.2.228	2002	*
Metabolism	Characterization of an S-layer glycoprotein produced in the course of S-layer variation of Bacillus stearothermophilus ATCC 12980 and sequencing and cloning of the sbsD gene encoding the protein moiety.	Egelseer EM, Danhorn T, Pleschberger M, Hotzy C, Sleytr UB, Sara M	Arch Microbiol	10.1007/s00203-001-0363-5	2001	*
Metabolism	Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms.	Jarosch M, Egelseer EM, Huber C, Moll D, Mattanovich D, Sleytr UB, Sara M	Microbiology (Reading)	10.1099/00221287-147-5-1353	2001	*
Metabolism	ISBst12, a novel type of insertion-sequence element causing loss of S-layer-gene expression in Bacillus stearothermophilus ATCC 12980.	Egelseer EM, Idris R, Jarosch M, Danhorn T, Sleytr UB, Sara M	Microbiology (Reading)	10.1099/00221287-146-9-2175	2000	*
Phylogeny	Bacillus thermodenitrificans sp. nov., nom. rev.	Manachini PL, Mora D, Nicastro G, Parini C, Stackebrandt E, Pukall R, Fortina MG	Int J Syst Evol Microbiol	10.1099/00207713-50-3-1331	2000	*
Metabolism	S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli.	Jarosch M, Egelseer EM, Mattanovich D, Sleytr UB, Sara M	Microbiology (Reading)	10.1099/00221287-146-2-273	2000	*
Stress	Predictive model to describe the combined effect of pH and NaCl on apparent heat resistance of Bacillus stearothermophilus.	Periago PM, Fernandez PS, Salmeron MC, Martinez A	Int J Food Microbiol	10.1016/s0168-1605(98)00111-1	1998	*
Metabolism	The S-layer proteins of two Bacillus stearothermophilus wild-type strains are bound via their N-terminal region to a secondary cell wall polymer of identical chemical composition.	Egelseer EM, Leitner K, Jarosch M, Hotzy C, Zayni S, Sleytr UB, Sara M	J Bacteriol	10.1128/JB.180.6.1488-1495.1998	1998	*
Enzymology	A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980.	Li Y, Triccas JA, Ferenci T	Biochim Biophys Acta	10.1016/s0167-4781(97)00103-6	1997	*
Metabolism	Evidence that an N-terminal S-layer protein fragment triggers the release of a cell-associated high-molecular-weight amylase in Bacillus stearothermophilus ATCC 12980.	Egelseer EM, Schocher I, Sleytr UB, Sara M	J Bacteriol	10.1128/jb.178.19.5602-5609.1996	1996	*
Pathogenicity	Effect of rubber stopper composition, preservative pretreatment and rinse water temperature on the moist heat resistance of Bacillus stearothermophilus ATCC 12980.	Rubio SL, Moldenhauer JE	PDA J Pharm Sci Technol		1995	*
Stress	Recovery of Heat-stressed Spores of Bacillus stearothermophilus on Solid Media Containing Calcium- and Magnesium-deficient Agar.	Sikes A, Whitfield S, Rosano DJ	J Food Prot	10.4315/0362-028X-56.8.706	1993	*
Enzymology	Construction of an Escherichia coli export-affinity vector for expression and purification of foreign proteins by fusion to cyclomaltodextrin glucanotransferase.	Hellman J, Mantsala P	J Biotechnol	10.1016/0168-1656(92)90097-s	1992	*
Enzymology	Localized insertion of new S-layer during growth of Bacillus stearothermophilus strains.	Gruber K, Sleytr UB	Arch Microbiol	10.1007/BF00446749	1988	*
Cultivation	Thermal inactivation and injury of Bacillus stearothermophilus spores.	Feeherry FE, Munsey DT, Rowley DB	Appl Environ Microbiol	10.1128/aem.53.2.365-370.1987	1987	*
Biotechnology	Production of PR Toxin and Roquefortine by Penicillium roqueforti Isolates from Cabrales Blue Cheese.	Medina M, Gaya P, Nunez M	J Food Prot	10.4315/0362-028X-48.2.118	1985	*
Pathogenicity	Rapid assay for tetracycline in premixes and mixed feeds.	Katz JM, Katz SE	J Assoc Off Anal Chem		1984	*
Enzymology	Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis.	Imanaka T, Fujii M, Aramori I, Aiba S	J Bacteriol	10.1128/jb.149.3.824-830.1982	1982	*
Pathogenicity	Rapid quantitative microbiological assay of antibiotics and chemical preservatives of a nonantibiotic nature.	Kabay A	Appl Microbiol	10.1128/am.22.5.752-755.1971	1971	*
Enzymology	Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus.	Ewis HE, Abdelal AT, Lu CD	Gene	10.1016/j.gene.2003.12.029	2004	*
	Influence of the incubation temperature and total dissolved solids concentration on the biofilm and spore formation of dairy isolates of Geobacillus stearothermophilus.	Kumar M, Flint S, Palmer J, Chanapha S, Hall C	Appl Environ Microbiol	10.1128/AEM.02311-20	2021	*
	Small-angle X-ray scattering for imaging of surface layers on intact bacteria in the native environment.	Sekot G, Schuster D, Messner P, Pum D, Peterlik H, Schaffer C	J Bacteriol	10.1128/JB.02164-12	2013	*
	Towards the structure of the C-terminal part of the S-layer protein SbsC.	Kroutil M, Pavkov T, Birner-Gruenberger R, Tesarz M, Sleytr UB, Egelseer EM, Keller W	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/S1744309109035386	2009	*
	A recombinant bacterial cell surface (S-layer)-major birch pollen allergen-fusion protein (rSbsC/Bet v1) maintains the ability to self-assemble into regularly structured monomolecular lattices and the functionality of the allergen.	Breitwieser A, Egelseer EM, Moll D, Ilk N, Hotzy C, Bohle B, Ebner C, Sleytr UB, Sara M	Protein Eng	10.1093/protein/15.3.243	2002	*
	Thermal Resistance of Bacillus stearothermophilus Heated at High Temperatures in Different Substrates.	Rodrigo F, Fernandez PS, Rodrigo M, Ocio MJ, Martinez A	J Food Prot	10.4315/0362-028X-60.2.144	1997	*
	D Values of Bacillus stearothermophilus Spores as a Function of pH and Recovery Medium Acidulant.	Fernandez PS, Gomez FJ, Ocio MJ, Rodrigo M, Sanchez T, Martinez A	J Food Prot	10.4315/0362-028X-58.6.628	1995	*

References

#2023

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22918

Anna E. Dinsdale, Gillian Halket, An Coorevits, Anita Van Landschoot, Hans-Jürgen Busse, Paul De Vos, Niall A. Logan: Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus. IJSEM 61: 1802 - 1810 2011 ( DOI 10.1099/ijs.0.025445-0 , PubMed 20817844 )

#23042

An Coorevits,Anna E. Dinsdale,Gillian Halket,Liesbeth Lebbe,Paul De Vos,Anita Van Landschoot,Niall A. Logan: Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.. IJSEM 62: 1470 - 1485 2012 ( DOI 10.1099/ijs.0.030346-0 , PubMed 21856988 )

#36276 ; Curators of the CIP;
#48397 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 26241

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67772 The University of Coimbra Bacteria Culture Collection (UCCCB) ; Curators of the UCCCB;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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