Strain identifier

BacDive ID: 1448

Type strain: No

Species: Geobacillus stearothermophilus

Strain Designation: C953

Strain history: CIP <- 1967, J.O. Clair, CNRS, Jouy en Josas, France: strain C 953, Bacillus stearothermophilus var. calidolactis

NCBI tax ID(s): 1422 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 817

BacDive-ID: 1448

DSM-Number: 1550

keywords: 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, rod-shaped

description: Geobacillus stearothermophilus C953 is an aerobe, spore-forming, thermophilic bacterium that was isolated from evaporated milk.

NCBI tax id

  • NCBI tax id: 1422
  • Matching level: species

strain history

@refhistory
817<- T.E. Galesloot, C953 (Bacillus stearothermophilus var. calidolactis)
350151967, J.O. Clair, CNRS, Jouy en Josas, France: strain C 953, Bacillus stearothermophilusvar. calidolactis
67770CIP 67.5 <-- J. O. Clair C 953.
123296CIP <- 1967, J.O. Clair, CNRS, Jouy en Josas, France: strain C 953, Bacillus stearothermophilus var. calidolactis

doi: 10.13145/bacdive1448.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Geobacillus
  • species: Geobacillus stearothermophilus
  • full scientific name: Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001

  • synonyms

    • @ref: 20215
    • synonym: Bacillus stearothermophilus

@ref: 817

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Geobacillus

species: Geobacillus stearothermophilus

full scientific name: Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001 emend. Coorevits et al. 2012

strain designation: C953

type strain: no

Morphology

cell morphology

  • @ref: 123296
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
817CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
35015MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123296CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
123296CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
123296CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
817positivegrowth55thermophilic
35015positivegrowth55thermophilic
51955positivegrowth55thermophilic
67770positivegrowth55thermophilic
123296positivegrowth45-55thermophilic
123296nogrowth10psychrophilic
123296nogrowth22psychrophilic
123296nogrowth30mesophilic
123296nogrowth37mesophilic

culture pH

  • @ref: 123296
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
51955aerobe
123296facultative anaerobe

spore formation

  • @ref: 123296
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
123296NaClpositivegrowth0-2 %
123296NaClnogrowth4 %
123296NaClnogrowth6 %
123296NaClnogrowth8 %
123296NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: Assay of Benzylpenicillin

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371salicin+builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
123296citrate-carbon source16947
123296esculin+hydrolysis4853
123296hippurate+hydrolysis606565
123296nitrate-reduction17632
123296nitrite+reduction16301
123296nitrate+respiration17632

metabolite production

  • @ref: 123296
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12329615688acetoin-
12329617234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
123296oxidase-
123296beta-galactosidase+3.2.1.23
123296alcohol dehydrogenase-1.1.1.1
123296gelatinase+/-
123296amylase+
123296DNase+
123296caseinase+3.4.21.50
123296catalase-1.11.1.6
123296tween esterase+
123296gamma-glutamyltransferase-2.3.2.2
123296lysine decarboxylase-4.1.1.18
123296ornithine decarboxylase-4.1.1.17
123296urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123296---+-----+--+--+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123296+/----+/-----+++++/-------+/----+/-++/-+++++/--++++--+-+------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
817evaporated milk
51955Milk,evaporated / coffee cream (NIZO)probably in Ede or HoornNetherlandsNLDEurope
67770Evaporated milk
123296Food, Milk

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_2201.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_191;97_207;98_231;99_2201&stattab=map
  • Last taxonomy: Geobacillus
  • 16S sequence: AB598739
  • Sequence Identity:
  • Total samples: 3216
  • soil counts: 387
  • aquatic counts: 603
  • animal counts: 2094
  • plant counts: 132

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
8171Risk group (German classification)
1232961Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Geobacillus stearothermophilus gene for 16S rRNA, partial sequence, strain: JCM 12216
  • accession: AB598739
  • length: 1481
  • database: ena
  • NCBI tax ID: 1422

External links

@ref: 817

culture collection no.: CCUG 34144, HNCMB 101025, LMG 11163, DSM 1550, JCM 12216, CIP 67.5, IAM 12043, JCM 14450, NBIMCC 2330, NBRC 100862, NCDO 1780, NCIMB 11780

straininfo link

  • @ref: 71098
  • straininfo: 12230

Reference

@idauthorscataloguedoi/urltitle
817Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1550)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1550
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35015Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10284
51955Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34144)https://www.ccug.se/strain?id=34144
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71098Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12230.1StrainInfo: A central database for resolving microbial strain identifiers
123296Curators of the CIPCollection of Institut Pasteur (CIP 67.5)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2067.5