Name: Bacillus subtilis 99-86
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 137923

Type strain:

Species: Bacillus subtilis

Strain Designation: 99-86

Culture col. no.: CIP 102199

Strain history: CIP <- 1986, Lab. Ident. Inst. Pasteur, Paris, France: strain 99-86 <- Saint Joseph Hosp., Paris, France

NCBI tax ID(s): 1423 (species)

Special Collections

Other strains from Bacillus subtilis

Section

Bacillus subtilis 99-86 is a facultative anaerobe, spore-forming, Gram-positive bacterium of the family Bacillaceae.

Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus subtilis**
	Full Scientific Name (LPSN)	Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

Synonym

"Vibrio subtilis"

Information on morphological and physiological properties Morphology

[Ref.: #38872]	Gram stain	positive

[Ref.: #38872]	Cell shape	rod-shaped

[Ref.: #38872]	Motility	yes

[Ref.: #38872]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #38872]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38872]

Culture medium growth

[Ref.: #38872]

Culture medium composition

expand / minimize

[Ref.: #38872]

Culture medium

CIP Medium 3

[Ref.: #38872]

Culture medium growth

[Ref.: #38872]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #38872]

Culture medium

CIP Medium 72

[Ref.: #38872]

Culture medium growth

[Ref.: #38872]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #38872]		growth	30 °C
[Ref.: #38872]		growth	22-37 °C
[Ref.: #38872]	no	growth	10 °C
[Ref.: #38872]	no	growth	45 °C
[Ref.: #38872]	no	growth	55 °C

	pH	Kind of pH		pH
[Ref.: #38872]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #38872]

Oxygen tolerance

facultative anaerobe

[Ref.: #38872]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #38872]		NaCl		growth	0-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #38872]	citrate ChEBI	+	carbon source
[Ref.: #38872]	esculin ChEBI	+	hydrolysis
[Ref.: #38872]	hippurate ChEBI	-	hydrolysis
[Ref.: #38872]	nitrate ChEBI	+	reduction
[Ref.: #38872]	nitrate ChEBI	+	respiration
[Ref.: #38872]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #38872]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #38872]		acetoin ChEBI	+
[Ref.: #38872]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #38872]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #38872]	amylase	+
[Ref.: #38872]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #38872]	caseinase	+	3.4.21.50
[Ref.: #38872]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #38872]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #38872]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #38872]	gelatinase	+
[Ref.: #38872]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #38872]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #38872]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #38872]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #38872]	oxidase	+
[Ref.: #38872]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #38872]	tween esterase	+
[Ref.: #38872]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #38872]	182	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	-	+	-	+	+	-	-	-	-	+	+	+	-	+	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	-	-	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #38872]	6944	-	+	+	+	-	-	-	-	-	-	+	-	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #38872]	Sample type/isolated from	Human, Sinus
[Ref.: #38872]	Geographic location (country and/or sea, region)	Paris
[Ref.: #38872]	Country	France
[Ref.: #38872]	Country ISO 3 Code	FRA
[Ref.: #38872]	Continent	Europe
[Ref.: #38872]	Isolation date	1986

[Ref.: #38872]	Geographic location (country and/or sea, region)	Paris
[Ref.: #38872]	Country	France
[Ref.: #38872]	Country ISO 3 Code	FRA
[Ref.: #38872]	Continent	Europe
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #38872]

Biosafety level

Risk group (French classification)

Availability in culture collections External links

[Ref.: #38872]

Culture collection no.

CIP 102199

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#38872 Collection of Institut Pasteur ; Curators of the CIP; CIP 102199

#68382

Automatically annotated from API zym .

* These data were automatically processed and therefore are not curated

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